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您的检索式：作者名="Chenpei Lu"
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| 1 | Satb2 expression in Foxci-promoted osteogenic differentiation of MC3T3-E1 cells is negatively regulated by microRNA-103-3p显示文摘The forkhead tran scripti on factor C1 (Foxci) is a cell-fate-determining factor that controls cranial bone development and osteogenic differentiation. Previously, it was demonstrated that various microRNAs (miRNAs) play important roles in osteogenesis and regulate the complex process of osteogenic differentiation. However, it remains unclear how miRNA expression changes during Foxci-promoted osteogenic differentiation. In this study, we successfully overexpressed the Foxci gene in MC3T3-E1 cells and investigated the alterations in the miRNA expression profile on day 3 after osteogenic induction by using a miRNA microarray. Nine downregulated miRNAs and eight upregulated miRNAs were found to be differentially expressed. Among these miRNAs, miR-103-3p was consistently downregulated in the Foxc7-overexpressing MC3T3-E1 cells and was identified as a negative regulator of osteogenic differentiation by using a gain-and Iose-of-funotion assay. The special AT-rich sequence-binding protein 2 (Satb2), a pivotal osteogenic transcription factor, was identified as the miR-103-3p targeting gene and was verified by real-time polymerase chain reaction, western blot analysis, and luciferase assay. Overexpression of miR-103-3p markedly inhibited the expression of Satb2 and attenuated Foxci-promoted osteogenic differentiation. Taken together, our results elucidated the miRNA expression profiles of MC3T3-E1 cells in the early stage of Foxc 1-promoted osteogenic differentiation and suggested that miR-103-3p acts as a negative regulator of the osteogenic differentiation of MC3T3-E1 cells by directly targeting Satb2. | Hongzhou Shen Chenpei Lu Jun Shi Hongliang Li Jiawen Si Guofang Shen | 2019 | Acta Biochimica et Biophysica Sinica2019，51，6： | 3 |
| 2 | Multi-physics analysis of the galvanic corrosion of Mg-steel couple under the influence of time-dependent anisotropic deposition film显示文摘The anisotropic deposit film formed during the galvanic corrosion can impede the mass transfer of the involved species,thereby affecting the electro-chemical behavior and the evolution of galvanic corrosion.The limitations of experimental studies in the spatial-temporal scales restrict a deeper understanding of the corrosion mechanism,which can be complemented by numerical simulation.A multi-physics coupled model is proposed in this work to systematically investigate the temporal and spatial evolution of galvanic corrosion of the Mg-steel couple with the growing anisotropic deposition layer.By utilizing the multi-physics field coupled technique,various coupled physical-chemical processes underlying the corrosion behavior are built into the model,including chemical reactions,ionic mass transfer in the bulk solution and the deposition layer,interfacial reaction,deposition of corrosion products as well as the morphological transitions caused by metal dissolution and deposition.In particular,the anisotropic deposit film is considered to be a porous layer with a porosity varying in time and space as the corrosion evolves.The predicted corrosion morphology by this model is better than the previous models.The coupled relationship between the electrochemical behavior(e.g.,electrode reaction kinetics,current density,surface potential)and the physical processes(e.g.,ionic transport,geometric evolution of metal surface and film interface)is revealed.The results indicate that a porous deposition layer with a denser inner layer and a loose outer layer is generated,leading to more significant inhibition of mass transfer in the inner layer than the outer layer.The anisotropism of the deposition layer results in a non-uniform conductivity distribution and a discontinuous current density distribution in the electrolyte.The current density on the electrode surface is inhibited by the deposition layer and the variation in the cathode/anode area ratio during the corrosion process.The competition between the transport process and the electrochemical reaction determines the spatial-temporal evolution of the ion concentration. | Kai Wang Chenpei Li Yanhui Li Jinling Lu Yueshe Wang Xingqi Luo | 2021 | Journal of Magnesium and Alloys2021，9，3： | 1 |
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