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		题名	作者	年代	出处	被引量
	1	MiR-181b suppresses proliferation of and reduces chemoresistance to temozolomide in U87 glioma stem cells显示文摘MicroRNAs regulate self renewal and differentiation of cancer stem cells.There,we sought to identify the expression of miR-181b in glioma stem cells and investigate the biological effect of miR-181b on glioma stem cells in this study.MiR-181b expression was measured by real-time PCR in glioma stem cells isolated from U87 cells by FACS sorting.After miR-181b was overexpressed in U87 glioma stem cells by miR-181b lentiviral expression vector and/or treatment of temozolomide,secondary neurosphere assay,soft agar colony assay and MTT assay were performed.Compared with U87 cells,the expression of miR-181b was significantly decreased in U87 glioma stem cells.Overexpression of miR-181b decreased neurosphere formation by U87 glioma stem cells in vitro and suppressed colony formation in soft agar,and the cell growth inhibition rates increased in a time-dependent manner in U87 glioma stem cells infected with miR-181b lentivirus.Furthermore,miR-181b had a synergistic effect on temozolomide-induced inhibition of secondary neurosphere and soft agar colony,and on cell growth inhibition rates.MiR-181b functions as a tumor suppressor that suppresses proliferation and reduces chemoresistance to temozolomide in glioma stem cells.	Ping Li Xiaoming Lu Yingyi Wang Lihua Sun Chunfa Qian Wei Yan Ning Liu Yongping You Zhen Fu	2010	The Journal of Biomedical Research2010，24，6：	14
	2	Functionalizing DNA nanostructures with natural cationic amino acids显示文摘Complexing self-assembled DNA nanostructures with various functional guest species is the key to unlocking new and exciting biomedical applications.Cationic guest species not only induce magnesium-free DNA to self-assemble into defined structures but also endow the final complex nanomaterials with new properties.Herein,we propose a novel strategy that employs naturally occurring cationic amino acids to induce DNA self-assembly into defined nanostructures.Natural L-arginine and L-lysine can readily induce the assembly of tile-based DNA nanotubes and DNA origami sheets in a magnesium-free manner.The self-assembly processes are demonstrated to be pH-and concentration-dependent and are achieved at constant temperatures.Moreover,the assembled DNA/amino acid complex nanomaterials are stable at a physiological temperature of 37◦C.Substituting L-arginine with its D form enhances its serum stability.Further preliminary examination of this complex nanomaterial platform for biomedical applications indicates that DNA/amino acids exhibit distinct cellular uptake behaviors compared with their magnesium-assembled counterparts.The nanomaterial mainly clusters around the cell membrane and might be utilized to manipulate molecular events on the membrane.Our study suggests that the properties of DNA nanostructures can be tuned by complexing them with customized guest molecules for a designed application.The strategy proposed herein might be promising to advance the biomedical applications of DNA nanostructures.	Dong Wang Chunfa Chen Qian Liu Qianwen Zhao Di Wu Yue Yuan Chaowang Huang Xiaorong Sun Chunji Huang David Tai Leong Guansong Wang Hang Qian	2021	Bioactive Materials2021，6，9：	0

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