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| 1 | Formation of uniform reduced graphene oxide films on modified PET substrates using drop-casting method显示文摘In this work,uniform reduced graphene oxide(RGO) films were formed on poly-(ethylene terephthalate)(PET) substrates using a simple drop-casting method.We investigated four types of substrates:unmodified PET,polydopamine-coated PET.carboxyl-group-modified PET,and alkyl-group-modified PET.Upon water evaporation,the surface of the polydopamine-modified PET substrates can interact with the reduced graphene oxide sheets to form flattened and continuous RGO films,which exhibit a sheet resistance of 21.75 kΩ/sq at 82%transmittance.The result indicates that the properties of the surface groups determined whether uniform and flattened RGO films could be formed on the substrates.Hence,we proposed a simple and effective way to produce transparent and conductive films in which the catechol unit exhibits a great effect on the deposition of uniform RGO films on PET substrates. | Chunlin Zhao Li Xing Junhui Xiang Lijie Cui JianbinJiao Huazheng Sai Zhenyou Li Fei Li | 2014 | Particuology2014，12，6： | 4 |
| 2 | Comprehensive identification and analyses of the Hsf gene family in the whole-genome of three Apiaceae species显示文摘Apiaceae is a major family from Apiales and includes many important vegetable and medicinal crops.Heat shock transcription factors(Hsf)play important roles in heat tolerance during plant development.Here,we conducted systematic analyses of the Hsf gene family in three Apiaceae species,including 17 Apium graveolens(celery),32 Coriandrum sativum(coriander),and 14 Daucus carota(carrot).A total of 73 Hsf genes were identified in three representative species,including Arabidopsis thaliana,Vitis vinifera,and Lactuca sativa.Whole-genome duplication played important roles in the Hsf gene family’s expansion within Apiaceae.Interestingly,we found that coriander had more Hsf genes than celery and carrot due to greater expansion and fewer losses.Twenty-seven branches of the phylogenetic tree underwent considerable positive selection in these Apiaceae species.We also explored the expression patterns of Hsf genes in three plant organs.Collectively,this study will serve as a rich gene resource for exploring the molecular mechanisms of heat tolerance.Additionally,this is the first study to report on the Hsf gene family in Apiaceae;thus,our research will provide guidance for future comparative and functional genomic studies on the Hsf gene family and others in Apiaceae. | Qiaoying Pei Tong Yu TongWu Qihang Yang Ke Gong Rong Zhou Chunlin Cui Ying Yu Wei Zhao Xi Kang Rui Cao Xiaoming Song | 2021 | Horticultural Plant Journal2021，7，5： | 3 |
| 3 | Glutamate synthase 1 is involved in iron-deficiency response and long-distance transportation in Arabidopsis显示文摘Iron is an essential microelement for plant growth.After uptake from the soil,iron is chelated by ligands and translocated from roots to shoots for sub-sequent utilization.However,the number of ligands in-volved in iron chelation is unclear.In this study,we identified and demonstrated that GLU1,which encodes a ferredoxin-dependent glutamate synthase,was involved in iron homeostasis.First,the expression of GLU1 was strongly induced by iron deficiency condition.Second,lesion of GLU1 results in reduced transcription of many iron-deficiency-responsive genes in roots and shoots.The mutant plants revealed a decreased iron concentration in the shoots,and displayed severe leaf chlorosis under the condition of Fe limitation,compared to wild-type.Third,the product of GLU1,glutamate,could chelate iron in vivo and promote iron transportation.Last,we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants.Overall,our results provide evidence that GLU1 is involved in iron homeo-stasis through affecting glutamate synthesis under iron deficiency conditions in Arabidopsis. | Man Cui Mengjun Gu Yaru Lu Yue Zhang Chunlin Chen Hong‐Qing Ling Huilan Wu | 2020 | Journal of Integrative Plant Biology2020，62，12： | 2 |
| 4 | Flow cytometric analysis of DNA content for four commercially important crabs in China显示文摘The genome size(C-value) of an organism is referring to the DNA content of its non-replicated haploid chromosome complement,generally deduced from measuring somatic diploid nuclei.We presented genome size(C-value) data obtained by flow cytometry for four commercially important crabs(Portunus trituberculatus,Charybdis japonica,Scylla paramamosain,and Eriocheir sinensis) common in the coast of China.Gallus domesticus(2C=2.5 pg) was used as the internal standard.The results showed that the C-value for P.trituberculatus,C.japonica,S.paramamosain,and E.sinensis were(2.31±0.01) pg,(2.33±0.03) pg,(1.64±0.02) pg,and(2.29±0.03) pg,respectively.The C-value of P.trituberculatus,C.japonica and S.paramamosain were reported for the first time.The data represented by the four species indicated that they had lower DNA contents than average DNA values in crustaceans((4.99±0.48) pg),and three of the four values were very similar if not identical.The results provide useful data for future studies in the fields of biodiversity,species conservation,and phylogeny of these commercial crabs.They will also be helpful in instructing the hybridization breeding program and estimating the cost of the whole genome sequencing project. | LIU Lei CUI Zhaoxia SONG Chengwen LIU Yuan HUI Min WANG Chunlin | 2016 | Acta Oceanologica Sinica2016，35，6： | 1 |
| 5 | Reproductive performance and offspring quality of the first and the second brood of female swimming crab, Portunus trituberculatus显示文摘 | Xugan Wu Yongxu Cheng Chaoshu Zeng Chunlin Wang Zhaoxia Cui | 2010 | Aquaculture2010，，1： | 1 |
| 6 | VEGFR2 inhibition hampers breast cancer cell proliferation via enhanced mitochondrial biogenesis显示文摘Objective:Vascular endothelial growth factor(VEGF),apart from its predominant roles in angiogenesis,can enhance cancer cell proliferation,but its mechanisms remain elusive.The purpose of the present study was therefore to identify how VEGF regulates cancer cell proliferation.Methods:VEGF effects on cancer cell proliferation were investigated with the VEGF receptor 2 inhibitor,Ki8751,and the breast cancer cell lines,MCF-7 and MDA-MB-231,using flow cytometry,mass spectrometry,immunoblotting,and confocal microscopy.Data were analyzed using one-way analysis of variance followed by Tukey’s multiple comparison test.Results:VEGF blockade by Ki8751 significantly reduced cancer cell proliferation,and enhanced breast cancer cell apoptosis.Mass spectrometric analyses revealed that Ki8751 treatment significantly upregulated the expression of mitochondrial proteins,suggesting the involvement of mitochondrial biogenesis.Confocal microscopy and flow cytometric analyses showed that Ki8751 treatment robustly increased the mitochondrial masses of both cancer cells,induced endomitosis,and arrested cancer cells in the high aneuploid phase.VEGFR2 knockdown by sh RNAs showed similar effects to those of Ki8751,confirming the specificity of Ki8751 treatment.Enhanced mitochondrial biogenesis increased mitochondrial oxidative phosphorylation and stimulated reactive oxygen species(ROS)production,which induced cancer cell apoptosis.Furthermore,Ki8751 treatment downregulated the phosphorylation of Akt and PGC1α,and translocated PGC1αinto the nucleus.The PGC1αalterations increased mitochondrial transcription factor A(TFAM)expression and subsequently increased mitochondrial biogenesis.Conclusions:VEGF enhances cancer cell proliferation by decreasing Akt-PGC1α-TFAM signaling-mediated mitochondrial biogenesis,ROS production,and cell apoptosis.These findings suggested the anticancer potential of Ki8751 via increased mitochondrial biogenesis and ROS production. | Hao Ni Min Guo Xuepei Zhang Lei Jiang Shuai Tan Juan Yuan Huanhuan L Cui Yanan Min Junhao Zhang Susanne Schlisio Chunhong Ma Wangjun Liao Monica Nister Chunlin Chen Shuijie Li Nailin Li | 2021 | Cancer Biology & Medicine2021，18，1： | 0 |
| 7 | Microbial-driven ectopic uranium extraction with net electrical energy production显示文摘The extraction of uranium (U) from U-bearing wastewater is of paramount importance for mitigating negative environmental impacts and recovering U resources. Microbial reduction of soluble hexavalent uranium (U(VI)) to insoluble tetravalent uranium (U(IV)) holds immense potential for this purpose, but its practical application has been impeded by the challenges associated with managing U-bacterial mixtures and the biotoxicity of U. To address these challenges, we present a novel spontaneous microbial electrochemical (SMEC) method that spatially decoupled the microbial oxidation reaction and the U(VI) reduction reaction. Our results demonstrated stable and efficient U extraction with net electrical energy production, which was achieved with both synthetic and real wastewater. U(VI) removal occurred via diffusion-controlled U(VI)-to-U(IV) reduction-precipitation at the cathode, and the UIVO_(2) deposited on the surface of the cathode contributed to the stability and durability of the abiotic U(VI) reduction. Metagenomic sequencing revealed the formation of efficient electroactive communities on the anodic biofilm and enrichment of the key functional genes and metabolic pathways involved in electron transfer, energy metabolism, the TCA cycle, and acetate metabolism, which indicated the ectopic reduction of U(VI) at the cathode. Our study represents a significant advancement in the cost-effective recovery of U from U(VI)-bearing wastewater and may open a new avenue for sustainable uranium extraction. | Xin Tang Yin Ye Chunlin Wang Bingqian Wang Zemin Qin Cui Li Yanlong Chen Yuheng Wang Zhiling Li Miao Lv Aijie Wang Fan Chen | 2024 | Frontiers of Environmental Science & Engineering2024，18，1： | 0 |
| 8 | Comparative analysis of long noncoding RNAs in angiosperms and characterization of long noncoding RNAs in response to heat stress in Chinese cabbage显示文摘Long noncoding RNAs(lncRNAs)are widely present in different species and play critical roles in response to abiotic stresses.However,the functions of lncRNAs in Chinese cabbage under heat stress remain unknown.Here,we first conducted a global comparative analysis of 247,242 lncRNAs among 37 species.The results indicated that lncRNAs were poorly conserved among different species,and only 960 lncRNAs were homologous to 524 miRNA precursors.We then carried out lncRNA sequencing for a genome-wide analysis of lncRNAs and their target genes in Chinese cabbage at different stages of heat treatment.In total,18,253 lncRNAs were identified,of which 1229 differentially expressed(DE)lncRNAs were characterized as being heat-responsive.The ceRNA network revealed that 38 lncRNAs,16 miRNAs,and 167 mRNAs were involved in the heat response in Chinese cabbage.Combined analysis of the cis-and trans-regulated genes indicated that the targets of DE lncRNAs were significantly enriched in the“protein processing in endoplasmic reticulum”and“plant hormone signal transduction”pathways.Furthermore,the majority of HSP and PYL genes involved in these two pathways exhibited similar expression patterns and responded to heat stress rapidly.Based on the networks of DE lncRNA-mRNAs,29 and 22 lncRNAs were found to interact with HSP and PYL genes,respectively.Finally,the expression of several critical lncRNAs and their targets was verified by qRT-PCR.Overall,we conducted a comparative analysis of lncRNAs among 37 species and performed a comprehensive analysis of lncRNAs in Chinese cabbage.Our findings expand the knowledge of lncRNAs involved in the heat stress response in Chinese cabbage,and the identified lncRNAs provide an abundance of resources for future comparative and functional studies. | Xiaoming Song Jingjing Hu Tong Wu Qihang Yang Xuehuan Feng Hao Lin Shuyan Feng Chunlin Cui Ying Yu Rong Zhou Ke Gong Tong Yu Qiaoying Pei Nan Li | 2021 | Horticulture Research2021，8，1： | 0 |
| 9 | Reading-related Brain Function Restored to Normal After Articulation Training in Patients with Cleft Lip and Palate:An fMRI Study显示文摘Cleft lip and/or palate(CLP)are the most common craniofacial malformations in humans.Speech problems often persist even after cleft repair,such that follow-up articulation training is usually required.However,the neural mechanism behind effective articulation training remains largely unknown.We used fMRI to investigate the differences in brain activation,functional connectivity,and effective connectivity across CLP patients with and without articulation training and matched normal participants.We found that training promoted task-related brain activation among the articulation-related brain networks,as well as the global attributes and nodal efficiency in the functional-connectivity-based graph of the network.Our results reveal the neural correlates of effective articulation training in CLP patients,and this could contribute to the future improvement of the post-repair articulation training program. | Liwei Sun Wenjing Zhang Mengyue Wang Songjian Wang Zhen Li Cui Zhao Meng Lin Qian Si Xia Li Ying Liang Jing Wei Xu Zhang Renji Chen Chunlin Li | 2022 | Neuroscience Bulletin2022，38，10： | 0 |
| 10 | miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1显示文摘Background:The egg production performance of chickens is affected by many factors,including genetics,nutrition and environmental conditions.These factors all play a role in egg production by affecting the development of follicles.MicroRNAs(miRNAs)are important non-coding RNAs that regulate biological processes by targeting genes or other non-coding RNAs after transcription.In the animal reproduction process,miRNA is known to affect the development and atresia of follicles by regulating apoptosis and autophagy of granulosa cells(GCs).Results:In this study,we identified potential miRNAs in the atretic follicles of broody chickens and unatretic follicles of healthy chickens.We identified gga-miR-30a-5p in 50 differentially expressed miRNAs and found that gga-miR-30a-5p played a regulatory role in the development of chicken follicles.The function of miR-30a-5p was explored through the transfection test of miR-30a-5p inhibitor and miR-30a-5p mimics.In the study,we used qPCR,western blot and flow cytometry to detect granulosa cell apoptosis,autophagy and steroid hormone synthesis.Confocal microscopy and transmission electron microscopy are used for the observation of autophagolysosomes.The levels of estradiol(E2),progesterone(P4),malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by ELISA.The results showed that miR-30a-5p showed a negative effect on autophagy and apoptosis of granulosa cells,and also contributed in steroid hormones and reactive oxygen species(ROS)production.In addition,the results obtained from the biosynthesis and dual luciferase experiments showed that Beclin1 was the target gene of miR-30a-5p.The rescue experiment conducted further confirmed that Beclin1 belongs to the miR-30a-5p regulatory pathway.Conclusions:In summary,after deep miRNA sequencing on healthy and atretic follicles,the results indicated that miR-30a-5p inhibits granulosa cell death by inhibiting Beclin1. | Haorong He Dongmei Li Yongtong Tian Qinyao Wei Felix Kwame Amevor Congjiao Sun Chunlin Yu Chaowu Yang Huarui Du Xiaosong Jiang Menggen Ma Can Cui Zhichao Zhang Kai Tian Yao Zhang Qing Zhu Huadong Yin | 2022 | Journal of Animal Science and Biotechnology2022，13，5： | 0 |
| 11 | HIGH T_c SUPERCONDUCTOR YBa_2 Cu_3 O_x SYNTHESIZED BY UNIDIRECTIONAL REACTION显示文摘A unidirectional reaction for synthesis of the high Tc superconductor YBa2Cu3Ox has beenproposed, Its Tc is 90-94.5 K and △T is 1 K. The phase relationships in the systemY2BaCuO5-Y2Cu2O5-CuO-YBa2Cu3Ox were studied and discussed. | FAN Zhanguo JI Chunlin WANG Kuihan CUI Chuanmeng LIU Sulan, Northeast University of Technology, Shenyang, China | 1989 | Acta Metallurgica Sinica(English Letters)1989，2，11： | 0 |
| 12 | Cryogenian and Ediacaran integrative stratigraphy,biotas,and paleogeographical evolution of the Qinghai-Tibetan Plateau and its surrounding areas显示文摘The complex evolutionary history of the Qinghai-Tibetan Plateau and its surrounding areas,including the continental blocks(Indian,Lhasa,South Qiangtang,Tarim,Olongbuluk,Central Qilian,Alxa,North China,Yangtze,Central Iran and Oman)and the orogenic belts between them,has long been the frontier in Earth science research.The Cryogenian and Ediacaran strata are extensively distributed in these blocks.Specifically,relatively complete Cryogenian and Ediacaran successions have been discovered in Oman,Indian,Yangtze,and Tarim blocks,while only the Ediacaran successions have been reported in Iran,the South Qiangtang,Central Qilian,Alxa,and North China blocks.Based on previous studies together with the integration of new materials and advancement obtained through the Second Tibetan Plateau Scientific Expedition and Research,this review aims to synthesize a correlative stratigraphic framework of the representative Cryogenian and Ediacaran sequences from the Qinghai-Tibetan Plateau and its surrounding areas.Furthermore,the Cryogenian and Ediacaran biotas and major geological events in these areas are comprehensively discussed in aspects of current research status.The results indicate that,in general,Ediacaran fossils of each area exhibit distinct features in preservation and assemblage composition,but the typical late Ediacaran fossils Cloudina and Shaanxilithes have been reported from most of these areas.In addition to the two global Cryogenian glaciations,late Ediacaran glaciogenic deposits are extensively recorded in the areas within and around the northern QinghaiTibetan Plateau(including the North China,Alxa,Central Qilian,Olongbuluk,and Tarim blocks,and the North Qilian Accretionary Belt),as well as central and southern Iran.However,further research is required to determine the age,distribution,and origin of these late Ediacaran glaciogenic deposits.Meanwhile,the middle Ediacaran DOUNCE/Shuram Excursion is widely documented in the Qinghai-Tibetan Plateau and its surrounding areas.The available data show that,after the break-up of the Rodinia supercontinent,most of the continental blocks in the areas were located along the northern margin of East Gondwana and a few(such as North China)were located between the Gondwana and Laurentia.In general,the paleogeographic evolution of most of these blocks during the Cryogenian and Ediacaran remains disputatious,necessitating further research to resolve the controversies surrounding their paleogeographic reconstruction models during this critical time interval. | Lang SUN Malik Muhammad Saud Sajid KHAN Chuan YANG Zhixin SUN Bing PAN Shehryar AHMED Lanyun MIAO Weichen SUN Chunlin HU Xiaojuan SUN Cui LUO Bo CHEN Zongjun YIN Fangchen ZHAO Guoxiang LI Maoyan ZHU | 2024 | Science China Earth Sciences2024，67，4： | 0 |
| 13 | 超选择子宫动脉与外周静脉灌注化疗宫颈癌组织内铂浓度的研究(英文)显示文摘Objective:The present study is to compare pharmacokinetics difference of carboplatin by using ultraselection uterine artery with by using peripheral vein in cervical cancer.Methods:Thirteen patients with locally advanced cervical cancer who had been proved by pathobiology were randomly divided into two groups:the ultraselection uterine artery group(group A, n = 6) and the peripheral vein(group B, n = 7).Carboplatin was administered by infusing into artery or vein in both groups at the dosage of 300 mg/m2.Tissues from the cervical tumor were collected at different times after infusion in both groups and then analyzed.Results:The peak concentration of platinum in tumor tissue was about 2.79 times higher in group A than that of group B(P < 0.05).The platinum concentrations in tumor in group A reached its peak levels immediately after infusion.But, group B had delayed time.While, for the time point of 0 min, when the administration finished immediately, the platinum concentration in tumor was significantly higher when compared with group B(P < 0.05).The tumor tissue area under the concentration(AUC) of carboplatin was about 2 times higher in group A than that of group B(P < 0.05).Conclusion:We observed the pharmacological advantages of chemotherapy by using ultraselection uterine artery administration of chemotherapeutic agent carboplatin in tumor tissue which provided theoretical basis and laboratory parameters of the intra-arterial chemotherapy for gynecologic malignancy. | Wei Wang Chunlin Chen Ping Liu Ben Ma Cui Liu Lani Chen | 2009 | The Chinese-German Journal of Clinical Oncology2009，8，5： | 0 |
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