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| 1 | Comparative studies of versatile extracellular proteolytic activities of lactic acid bacteria and their potential for extracellular amino acid productions as feed supplements显示文摘Background:Increasing understanding on the functions of amino acids (AA) has led to new commercial applications and expansion of the worldwide markets.However,the current technologies rely heavily on non-food grade microorganism and chemical synthesis for the production of AA.Several studies reported that lactic acid bacteria (LAB) have the capability of producing AA owing to their well-established proteolytic system and amino acid biosynthesis genes.Hence,the objectives of this study were to explore the extracellular proteolytic activity of LAB isolated from various Malaysian fermented foods and their potential to produce AA extracellularly as feed supplements.Results:All the studied LAB isolates were versatile extracellular protease producers,whereby extracellular protease activities were detected from acidic to alkaline pH (pH 5,pH 6.5,pH 8) using qualitative and quantitative proteolytic assays.The highest proteolytic activity at pH 5 (15.76 U/mg) and pH 8 (19.42 U/mg) was achieved by Lactobacillus plantarum RG14,while Lactobacillus plantarum RS5 exhibited the highest proteolytic activity of 17.22 U/mg at pH 6.5.As for the results of AA production conducted in de Man,Rogosa and Sharpe medium and analysed by high pressure liquid chromatography system,all LAB isolates were capable of producing an array of AA.Generally,Pediococcus sp.showed greater ability for AA production as compared to Lactobacillus sp.Moreover,the studied LAB were able to produce a few major feed supplement AA such as methionine,lysine,threonine and tryptophan.P.pentosaceus TL-3 recorded the highest methionine and threonine productivity of 3.72 mg/L/h and 5.58 mg/L/h respectively.However,L.plantarum I-UL4 demonstrated a lysine productivity of 1.24 mg/L/h,while P.acidilactici TP-6 achieved up to 1.73 mg/L/h of tryptophan productivity.Conclusion:All the 17 studied LAB isolates possessed versatile extracellular proteolytic system and have vast capability of producing various amino acids including a few major feed supplement AA such as methionine,lysine,threonine and tryptophan.Despite AA production was strain dependent,the studied LAB isolates possessed vast potential and can be exploited further as a bio-agent or an alternative amino acids and bioactive peptide producers. | Ye Heng Lim Hooi Ling Foo Teck Chwen Loh Rosfarizan Mohamad Norhani Abdullah | 2019 | Journal of Animal Science and Biotechnology2019，10，3： | 5 |
| 2 | Frequency acqusition and tracking in high dynamic environments 显示文摘 | Su Yu T Wu Ru Chwen | 2000 | IEEE Transaction on Vehicular Technology2000，49，6： | 1 |
| 3 | Subcooled Flow Boiling Heat Transfer of R-134a and Bubble Characteristics in a Horizontal Annular Duct显示文摘 | Chih-Ping Yina Yi-Yie Yana Tsing-Fa Lina Bing- Chwen Yangb | 2000 | Int J of Heat Mass Transfer2000，43，： | 1 |
| 4 | Application of the Theory of Planned Behavior to Green Hotel Choice:Testing the Effect of Environmental Friendly Activities显示文摘 | Heesup Han Li-Tzang(Jane)Hsu Chwen Sheu | 2010 | Tourism Management2010，31，3： | 1 |
| 5 | A model for reverse logistics entry by third-party providers显示文摘 | Dennis W K Chwen S | 2002 | Omega2002，30，5： | 1 |
| 6 | A model for reverse logistics entry by third-party providers 显示文摘 | Dennis W Krumwiede Chwen Sheu | 2002 | Omega2002，，5： | 1 |
| 7 | A model for reverse logistics entry by third-party providers 显示文摘 | Krumwiede D W Shen Chwen | 2002 | Omega2002，30，5： | 1 |
| 8 | Liquid chromatographic method with amperometric detection to determine acteoside in rat blood and brain microdialysates and its application to pharmacokinetic study显示文摘 | Yu Tse Wu Tong Rang Tsai Lie Chwen Lin | | 0，，： | 1 |
| 9 | A model for reverse logistics entry by third-party providers 显示文摘 | Dennis W Krumwiede Chwen Sheu | 2002 | Omega2002，，30： | 1 |
| 10 | A Model for Reverse Logistics Entry by Third - Party providers 显示文摘 | Dennis W Krumwiede Chwen Sheu | 2002 | Omega2002，30，5： | 1 |
| 11 | Prevalence of antibiotic resistance in lactic acid bacteria isolated from the faeces of broiler chicken in Malaysia显示文摘 | Shazali Nurhazirah Foo Hooi Ling Loh Teck Chwen | 2014 | Gut patho- gens2014，6，1： | 1 |
| 12 | Psychiatric morbidity and posttraumatic symptoms among earthquake victims in primary care clinics显示文摘 | Yen Kuang Yang Tzung Lieh Yeh Chwen cheng chen | 2003 | General Hospital Psychiatry2003，25，： | 1 |
| 13 | A Model for Reverse Logistics Entry by Third-party Providers显示文摘 | Chwen Sheub | 2002 | The international Journal of Management Science2002，30，5： | 1 |
| 14 | A model for rever logistics entry by third-party providers显示文摘 | Dennis W Krumwiede Chwen Sheu | 2002 | International Journal of Management Science2002，30，： | 1 |
| 15 | Antibacterial activity of leaf essential oils and their constituents from Cinnamomum osmophloeum显示文摘 | S T Chang Pin Fun Chen Shan Chwen Chang | 2001 | Journal of Ethnopharmacology2001，77，： | 1 |
| 16 | A Model for Reverse Logistics Entryby Third-party Providers显示文摘 | Krumwiede DW Chwen Sheu | 2002 | Omega2002，30，： | 1 |
| 17 | Application of the Theory of Planned Behavior to green hotel choice : Testing the effect of en- vironmental friendly activities 显示文摘 | Heesup Han Li-Tzang (Jane) Hsu Chwen Sheu | 2010 | Tourism Management2010，31，： | 1 |
| 18 | Application of the Theory of Planned Behavior to green hotel choice : Testing the effect of environ-mental friendly aetivities显示文摘 | Heesup Han Li-Tzang Hsu Chwen Sheu | 2010 | Tourism Management2010，31，2： | 1 |
| 19 | A Model fo Reverse Logistics Entry by Third-party Providers显示文摘 | Krumwiede D W Chwen Sheu | 2002 | Omega2002，30，： | 1 |
| 20 | Integrating ABC and TOC for better manufacturing decision making 显示文摘 | Chwen Sheu Ming-Hsiang Chen Stacy Kovar | 2007 | Integrated Manufacturing systtms2007，14，5： | 1 |
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