6篇
您的检索式：作者名="Clark Matthew C"
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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | MRI segmentation using fuzzy clustering techniques显示文摘 | Clark Matthew C Hall Lawrence O | 1994 | IEEE Engineering in Medicine and Biology1994，13，5： | 1 |
| 3 | Automatic Tumor Segmentation Using knowledge-Based Technique显示文摘 | Clark Matthew C Hall Lawrence O | 1998 | IEEE Transactions on Medical Imaging1998，17，2： | 1 |
| 4 | Intratumor heterogeneity and branched evolution revealed by multiregion sequencing显示文摘 | Gerlinger M Rowan AJ Horswell S Larkin J Endesfelder D Gronroos E Martinez P Matthews N Stewart A Tarpey P Varela I Phillimore B Begum S McDonald NQ Butler A Jones D Raine K Latimer C Santos CR Nohadani M Eklund AC Spencer-Dene B Clark G Pickering L Stamp | | 0，，10： | 1 |
| 5 | Automatic tumor segmentation using nowledge-based technique显示文摘 | Matthew C Clark Lawrence O Hall Dmirty B Goldgof | 2006 | IEEE Trans Medical Imaging2006，19，2： | 1 |
| 6 | Automatic Tumor Segmentation Using knowledge - Based Technique 显示文摘 | Clark Matthew C Hall Lawrence O | 2001 | IEEE Transactions on Medical Imaging2001，17，2： | 1 |
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