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| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | Metabolic and Vascular Factors in the Parhogenesis of Diabetes Neuropathy显示文摘 | CAMREON N E COTTER M A | 1997 | Diabetes1997，46，2： | 1 |
| 3 | Prenatal diagnosis of a fetus with two balanced de nov{} chromosome rearrangements显示文摘 | Cotter PD Caggana M Willner JP | 1996 | Am J Med Genet1996，66，2： | 1 |
| 4 | Effects of diabetes and treatment with the antioxidant α-lipoic acid on endotheli-al and neurogenic responses of corpus cavernosum in rats显示文摘 | Keegan A Cotter M A Cameron N E | 1999 | Diabetologia1999，42，： | 1 |
| 5 | Melanocyte expression of survivin promotes development and metastasis of UV-induced melanoma in HGF-transgenic mice 显示文摘 | Thomas J Liu T Cotter M A | 2007 | Cancer Res2007，67，11： | 1 |
| 6 | Structure of Very High Thermal Conductance显示文摘 | Grover G M Cotter T P Erikson G F | | 0，，06： | 1 |
| 7 | Metabolic and vascular factors in the pathogenesis of diabetic neuropathy显示文摘 | Cameron N E Cotter M A | 1997 | Diabetes1997，46，2： | 1 |
| 8 | A randomized clinical tri- al of vision therapy/orthoptics versus pencil pushups for the treat- ment of convergence insufficiency in young adults 显示文摘 | Scheiman M Mitchell GL Cotter S et d | 2005 | Optom Vis Sci2005，82，7： | 1 |
| 9 | MCMC methods for functions:modifying old algorithms to make them faster显示文摘 | COTTER S L ROBERTS G O STUART A M | 2013 | Statistical Science2013，28，3： | 1 |
| 10 | Mechanism regulating reactive oxygen species in tumor-induced myeloid-derived suppressor cells显示文摘 | Corzo C A Cotter M J Cheng P | 2009 | J Immunol2009，182，9： | 1 |
| 11 | Disturbance of Notch-1 and Wnt signalling proteins in neuroglial balloon cells and abnormal large neurons in focal cortical dysplasia in human cortex 显示文摘 | Cotter D Honavar M Lovestone S | 1999 | Acta Neuropathol ( Berl)1999，98，5： | 1 |
| 12 | Treatment of persistent corneal ep- ithelial defect with extended wear of a fluid-ventilated gas-perme- able scleral contact lens 显示文摘 | Rosenthal P Cotter J M Baum J | 2000 | Am J Ophthalmol2000，130，1： | 1 |
| 13 | Vascular factors and metabolic interactions in the pathogenesis of diabetic neuropathy显示文摘 | Cameron N E Eaton S E Cotter M A | 2001 | Diabetologia2001，44，11： | 1 |
| 14 | Candida parapsilosis menin- gitis associated with Gliadel (BCNU) wafer implants 显示文摘 | OBrien D Cotter M Lim CH | 2011 | Br J Neurosurg2011，25，2： | 1 |
| 15 | The role of the kidney in heart failure显示文摘 | Metra M Cotter G Gheorghiade M | 2012 | Eur Heart J2012，33，17： | 1 |
| 16 | Effects of diabetes on reactivity of sciatic vasa nervo run in rats显示文摘 | Maxfield E K Cameron N E Cotter M A | 1997 | J Diabetes Comp l1997，11，1： | 1 |
| 17 | Cell death(apoptosis) in cell culture systems显示文摘 | Cotter TG AL Rubeai M | 1995 | Trends Biotechnol1995，13，： | 1 |
| 18 | Lack of aspirin effect : aspirin resistance or resistance to taking aspirin? 显示文摘 | Cotter G Shemesh E Zehavi M | 2004 | Am Heart J2004，147，2： | 1 |
| 19 | Effect of serelaxin on cardiac,renal,and hepatic biomarkers in the Relaxin in Acute Heart Failure(RELAX-AHF)development program:correlation with outcomes显示文摘 | Metra M Cotter G Davison BA | | 0，，02： | 1 |
| 20 | Towards understanding the cause of stroke in young adults utilising a new stroke classification system( A - S - C - O) 显示文摘 | Cotter PE Belham M Martin PJ | 2012 | Cerebrovasc Dis2012，33，2： | 1 |
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