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您的检索式：作者名="Dafeng Liang"
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| 1 | Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis显示文摘Extracellular polymeric substances(EPSs)are extracellular macromolecules in bacteria,which function in cell growth and show potential for mechanism study and biosynthesis application.However,the biosynthesis mechanism of EPS is still not clear.We herein chose Bacillus licheniformis CGMCC 2876 as a target strain to investigate the EPS biosynthesis.epsK,a member of eps cluster,the predicted polysaccharide synthesis cluster,was overexpressed and showed that the overexpression of epsK led to a 26.54%decrease in the production of EPS and resulted in slenderer cell shape.Transcriptome analysis combined with protein-protein interactions analysis and protein modeling revealed that epsK was likely responsible for the synthesis of teichuronic acid,a substitute cell wall component of teichoic acid when the strain was suffering phosphate limitation.Further cell cultivation showed that either phosphate limitation or the overexpression of teichuronic acid synthesis genes,tuaB and tuaE could similarly lead to EPS reduction.The enhanced production of teichuronic acid induced by epsK overexpression triggered the endogenous phosphate starvation,resulting in the decreased EPS synthesis and biomass,and the enhanced bacterial chemotaxis.This study presents an insight into the mechanism of EPS synthesis and offers the potential in controllable synthesis of target products. | Yiyuan Xu Lijie Yang Haiyan Wang Xiaoyu Wei Yanyan Shi Dafeng Liang Mingfeng Cao Ning He | 2022 | Synthetic and Systems Biotechnology2022，7，2： | 1 |
| 2 | Two Cases of COVID-19 with Persistently Positive SARS-CoV-2- Specific IgM During One-Year Follow-Up-Sichuan Province,China,February 2021显示文摘On January 22,2020,a 41-year-old male(Patient A)developed a fever with a maximum temperature of 38°C after returning to Chengdu City from Wuhan City.He was admitted to the Public Health Clinical Center of Chengdu on January 26,2020,after being confirmed to be positive for coronavirus disease 2019(COVID-19)by Chengdu CDC.Chest computed tomography(CT)revealed multiple plaque-like,ground-glass shadows in the upper,middle,and lower lobes of the right lung and upper and lower lobes of the left lung,as well as a small amount of pericardial effusion. | Bennan Zhao Yuezhu Chen Yong Yue Dafeng Liu Guihui Wu Yi Mao Liang Wang Zhu Liu | 2021 | China CDC weekly2021，3，46： | 0 |
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