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| 1 | Subacute ruminal acidosis in dairy herds:Microbiological and nutritional causes,consequences,and prevention strategies显示文摘Dairy cattle are frequently fed high-concentrate(HC)diets in modern intensive feeding systems,especially in the transition period.During this period,cows face many alterations that include hormonal changes and shifting to a lactating state.Switching to a HC diet that may disrupt the ruminal microbiota balance can lead to subacute ruminal acidosis(SARA).Moreover,the main factor shaping the rumen microbiota is dietary composition,especially the ratio of starch to fibrous carbohydrates.Feeding highly fermentable carbohydrate diets after adaptation to forage diets leads to a rumen fermentation rate that exceeds rumen absorption and buffering rates,resulting in a reduction in ruminal pH.As a result of Gram-negative bacterial cell lysis,an increase in harmful ruminal bacterial metabolites,including lipopolysaccharide,lactic acid,and histamine,is observed.The interactions between the host immune system and the ruminal microbiota play an essential role in many physiological processes and the development of the disorder.Progress in DNA sequencing and bioinformatics platforms provides new opportunities to investigate the composition of ruminal microbes and yields unique advances in understanding ecology of the rumen.Subacute ruminal acidosis is linked with a change in the ruminal microbiota structure and richness and with other metabolic disorders;such as rumenitis,milk fat depression,laminitis,and liver abscesses.Therefore,this review aims to explore a better understanding of the crosstalk between diet and microbiota in the prevalence of rumen acidosis and its consequences,which is crucial for control strategies such as feeding management,and supplementation with thiamine,prebiotics,and probiotics. | Mawda E.Elmhadi Darien K.Ali Mawahib K.Khogali Hongrong Wang | 2022 | Animal Nutrition2022，，3： | 4 |
| 2 | 蓝舌病毒VP5蛋白抗体的制备及鉴定显示文摘本研究参考GenBank中蓝舌病毒16型(BTV-16)标准株RSAvvvv的序列,首先人工合成编码BTV-16 VP5蛋白的S6全长基因,并克隆于pUC-57载体。设计表达引物,利用PCR方法扩增N末端缺少编码第1~41位氨基酸的截短基因VP5Δ41aa,将其亚克隆到表达载体pET-32a(+)中,构建重组表达质粒pET-VP5Δ41aa。将表达质粒转化大肠杆菌BL21(DE3)后,利用IPTG进行诱导表达,SDS-PAGE结果显示,重组蛋白VP5Δ41aa在大肠杆菌中大量表达。利用组氨酸标签纯化树脂获得了高纯度的VP5Δ41aa蛋白,用其3次免疫新西兰大白兔制备相应的抗血清,Western-blot检测显示,抗血清不仅可与重组表达的VP5Δ41aa蛋白反应,而且可与BTV感染细胞中的VP5蛋白反应。免疫荧光试验表明,该抗体也可与BTV感染C6/36细胞中具有天然构象的VP5蛋白反应。上述结果表明,本试验制备的VP5抗体将为深入研究VP5蛋白的生物学功能奠定基础。 | 康棣 独军政 高闪电 田占成 Darien Kheder ALI MOHAMED 郭艳妮 刘光远 罗建勋 殷宏 | 2018 | 中国兽医科学2018，48，6： | 3 |
| 3 | 蓝舌病毒NS2蛋白的抗体制备及鉴定显示文摘本研究参考GenBank中蓝舌病毒16型(Bluetongue virus serotype 16,BTV-16)标准株RSAvvvv的序列,人工合成编码BTV-16 NS2蛋白的S8基因,将其亚克隆至原核表达载体pPRoEx-HTb中,构建重组表达质粒pPro-NS2,并转化大肠杆菌BL21进行IPTG诱导表达,并进行可溶性分析。SDS-PAGE结果显示,表达的rNS2蛋白主要以包涵体的形式存在于菌体中。利用组氨酸标签纯化树脂获得了高纯度的NS2蛋白,免疫新西兰白兔制备抗NS2蛋白的多克隆抗体。Western blot结果显示,制备的抗NS2蛋白多克隆抗体不仅可与重组表达的rNS2蛋白反应,而且可与BTV感染细胞后的天然NS2蛋白反应。间接免疫荧光结果显示,该抗体也可与BTV感染C6/36细胞中的天然NS2蛋白反应,且发现NS2大量分布于C6/36细胞的胞质中。本研究所制备的NS2蛋白的多克隆抗体具有很好的反应性和特异性,为进一步研究NS2蛋白的功能奠定了基础。 | 张国芮 独军政 高闪电 田占成 Darien Kheder Ali Mohamed 康棣 郭艳妮 薛慧文 殷宏 | 2019 | 中国动物传染病学报2019，27，2： | 3 |
| 4 | Piezoelectric windmill: a novel solution to remote sensing显示文摘 | Pfiya S Chen Chih-ta Fye Darien | 2005 | Japanese Journal of Applied Physics2005，44，3： | 1 |
| 5 | Adhesion signaling-crosstalk between integrins, Src and Rho显示文摘 | Huveneers S Darien EH | 2009 | J Cell Sci2009，122，8： | 1 |
| 6 | PTEN interactions with focal adhesion kinase and suppression of the extracellular matrix dependent phosphatidylinositol 3-kinade/Akt cell survival pathway 显示文摘 | TamuraM Gn J Darien EH | 1999 | J Biol Chem1999，274，20： | 1 |
| 7 | Paravertebral block: new bene-fits from an old procedure显示文摘 | Hector V Jinhong L Darien K | 2007 | Current Opinion in Anaesthesiology2007，20，4： | 1 |
| 8 | Immediate effects of positive end-expiratory pressure and low and high tidal volume venfilalion upon gas exehang and compliance in patients with acute lung injury 显示文摘 | Darien B Thomas S Kenneth H | 2001 | J Trauma2001，51，6： | 1 |
| 9 | 蓝舌病病毒VP7蛋白的可溶性纯化及多克隆抗体的制备显示文摘首先参照GenBank中已发表的基因序列人工合成蓝舌病病毒VP7基因,将其克隆到表达载体pSMK上。选取含有正确开放阅读框的重组质粒pSMK-VP7,转化大肠杆菌BL21感受态细胞,于16℃利用IPTG诱导剂进行诱导表达,优化表达条件,纯化重组VP7蛋白,免疫新西兰白兔制备VP7多克隆抗体,利用免疫印迹试验对制备的多克隆抗体进行特异性分析。结果显示,重组VP7蛋白在大肠杆菌中呈可溶性表达,且16 h表达量最大,利用镍离子树脂纯化获得了高纯度的VP7蛋白;原核表达的重组VP7以及天然的VP7蛋白均能够与VP7多克隆抗体发生特异性反应。本研究成功实现了重组BTV VP7蛋白的可溶性表达和纯化,并制备了特异性良好的多克隆抗体,为进一步开展BTV的VP7功能研究和建立BTV诊断方法奠定了基础。 | 丁晓攀 车永军 郭艳妮 高闪电 田占成 ALI MOHAMED Darien Kheder 关贵全 孙世琪 殷宏 独军政 | 2021 | 中国兽医科学2021，51，9： | 1 |
| 10 | Low molecular weight heparin prenvent the pulmonary hemodynamic and pathomorphologic effects of endotoxin in a porcine acute lung injury model显示文摘 | Darien BJ Fareed J Ccentgraf KS | 1998 | Shock1998，9，4： | 1 |
| 11 | Lipoprotein NMB0928 from Neisseria meningitidis serogroup B as a novel vaccine candidate显示文摘 | Maité Delgado Daniel Yero Olivia Niebla Sonia González Yanet Climent Yusleydis Pérez Karem Cobas Evelín Caballero Darien García Rolando Pajón | 2007 | Vaccine2007，，50： | 1 |
| 12 | Apoptin induces apoptosis in human transformed and malignant cells but not in normal cells显示文摘 | Darien - Van Oorschot AA Fischer DF Grimbergen JM | 1997 | Proc Natl Acad Sci U S A1997，94，11： | 1 |
| 13 | The effect of Bcl- 2 on Apoptin in normal'vs transformed human cells 显示文摘 | Darien - Van Oorschot AA Zhang Y Erkeland SI | 1999 | Leukemia1999，13，1： | 1 |
| 14 | Importance of nuclear localization of apoptin for tumor - specific Induction of apoptosis 显示文摘 | Darien - Van Oorschot AA Zhang YH Leliveld SR | 2003 | J Biol Chem2003，278，27： | 1 |
| 15 | Equine PSGL-1 modifications required for P-selectin binding显示文摘 | Jin Xu Jun Cai M. Suresh Simon F. Peek Benjamin J. Darien | 2009 | Veterinary Immunology and Immunopathology2009，，1： | 1 |
| 16 | Integrins: signaling, disease, and therapy显示文摘 | Huveneers S Truong H Darien HJ | 2007 | Int J Radiat Biol2007，83，： | 1 |
| 17 | The chicken anemia virus - derived protein apoptin requires activation of caspases for induction of apoptosis in human tumor cells显示文摘 | Darien - van Oorschot AA van Der Eb A J Noteborn MH | 2000 | J Virol2000，74，15： | 1 |
| 18 | Low molecular weight heparine prevents the pulmonary hemodynamic and pathomorphologic effects of endotoxin in a porcine acute lung injury model显示文摘 | Darien BJ Fareed J Centgraf KS | 1998 | Shock1998，9，： | 1 |
| 19 | Low molecular weight heparin prevents the pulmonary hemodynamic and pathomorphologic effects of endotoxin in a porcine acute lung injury model显示文摘 | FAREED J CENTGRAF K S | 1998 | Shock1998，9，4： | 1 |
| 20 | Method of Preparing A Polyurethane显示文摘 | Groszos S J Darien Drechsel E K | 1957 | US: P2802022(1957)1957，，28： | 1 |
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