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| 1 | A Probe into China's Near Term Finished Petroleum Products Market显示文摘自从 2008 ,这篇文章在中国在完成的石油产品的消费描述了变化,指颠倒了完成的油产品,和中国状况和油定价政策和主要因素与在工业 sectors.This 的变化联系了的国内经济的消费的国际经济环境文章,由在未来市场趋势上做设计,估计了在可预见的未来。 | Duan Yanting Yin Zhe (Beijing University of Technology,Beijing 100124) | 2009 | China Petroleum Processing & Petrochemical Technology2009，11，3： | 0 |
| 2 | Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength显示文摘The intrinsic terminator in prokaryotic forms secondary RNA structure and terminates the transcription.However,leaking transcription is common due to varied terminator strength.Besides of the representative hairpin and U-tract structure,detailed sequence and thermodynamic features of terminators were not completely clear,and the effect of terminator on the upstream gene expression was unclearly.Thus,it is still challenging to use terminator to control expression with higher precision.Here,in E.Coli,we firstly determined the effect of the 3′-end sequences including spacer sequences and terminator sequences on the expression of upstream and downstream genes.Secondly,terminator mutation library was constructed,and the thermodynamic and sequence features differing in the termination efficiency were analyzed using the FlowSeq technique.The result showed that under the regulation of terminators,a negative correlation was presented between the expression of upstream and downstream genes(r=0.60),and the terminators with lower free energy corelated with higher upstream gene expression.Meanwhile,the terminator with longer stem length,more compact loop and perfect U-tract structure was benefit to the transcription termination.Finally,a terminator strength classification model was established,and the verification experiment based on 20 synthetic terminators indicated that the model can distinguish strong and weak terminators to certain extent.The results help to elucidate the role of terminators in gene expression,and the key factors identified are crucial for rational design of terminators,and the model provided a method for terminator strength prediction. | Weiji Zhai Yanting Duan Xiaomei Zhang Guoqiang Xu Hui Li Jinsong Shi Zhenghong Xu Xiaojuan Zhang | 2022 | Synthetic and Systems Biotechnology2022，7，4： | 0 |
| 3 | Characterization and implications of prokaryotic ribosome-binding sites across species显示文摘The ribosome-binding site(RBS)in the 5′untranslated region is recognized by 16S rRNA to start translation and is an essential element of the gene expression system.RBSs have been widely applied in regulating gene expression in various scenarios,including Gram-negative or Gram-positive bacteria.Here,we first rationally designed and constructed an RBS mutant library containing 66 RBSs.The strength of these RBSs in E.coli and C.glutamicum was characterized individually.The RBS strength spanned about 200 and 15 times in the two species,respectively.The strength of RBSs in C.glutamicum was generally lower than that of in E.coli.A total of 18 RBSs showed similar strength(within twofold differences)between the species in our study,and the correlation analysis of the strength of RBSs between E.coli and C.glutamicum(R^(2)=0.7483)revealed that these RBSs can be used across species.The sequence analysis revealed that the RBS region with two Ts stated was beneficial for RBS to function cross-species.The RBS characterized here can be used to precisely regulate gene expression in both hosts,and the characteristics of cross-species RBSs provide basic information for RBS rational design. | Yanting Duan Xiaojuan Zhang Weiji Zhai Jinpeng Zhang Jiawei Ren Xiaomei Zhang Guoqiang Xu Jinsong Shi Zhenghong Xu | 2022 | Systems Microbiology and Biomanufacturing2022，2，4： | 0 |
| 4 | Discovery of a subtype-selective, covalent inhibitor against palmitoylation pocket of TEAD3显示文摘The TEA domain(TEAD)family proteins(TEAD1-4)are essential transcription factors that control cell differentiation and organ size in the Hippo pathway.Although the sequences and structures of TEAD family proteins are highly conserved,each TEAD isoform has unique physiological and pathological functions.Therefore,the development and discovery of subtype selective inhibitors for TEAD protein will provide important chemical probes for the TEAD-related function studies in development and diseases.Here,we identified a novel TEAD 1/3 covalent inhibitor(DC-TEADin1072)with biochemical IC50 values of 0.61±0.02 and 0.58±0.12μmol/L against TEAD1 and TEAD3,respectively.Further chemical optimization based on DC-TEAD in 1072 yielded a selective TEAD3 inhibitor DCTEAD3 in03 with the IC_(50) value of 0.16±0.03μmol/L,which shows 100-fold selectivity over other TEAD isoforms in activity-based protein profiling(ABPP)assays.In cells,DC-TEAD3 in03 showed selective inhibitory effect on TEAD3 in GAL4-TEAD(1-4)reporter assays with the IC50 value of1.15μmol/L.When administered to zebrafish juveniles,experiments showed that DC-TEAD3 in03 reduced the growth rate of zebrafish caudal fins,indicating the importance of TEAD3 activity in controlling proportional growth of vertebrate appendages. | Tian Lu Yong Li Wenchao Lu TWGM Spitters Xueyu Fang Jun Wang Simian Cai Jing Gao Yanting Zhou Zhe Duan Huan Xiong Liping Liu Qi Li Hualiang Jiang Kaixian Chen Hu Zhou Hua Lin Huijin Feng Bing Zhou Christopher L.Antos Cheng Luo | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 0 |
| 5 | Case report显示文摘1 History A 48-year-old male has suffered from epilepsy for 7 months.2 Image Findings Axial precontrast T2-weighted MR image shows multiple round cysts with well-defined margin in the right temporal and occipital lobe. | Duan Yanting | 2019 | 功能与分子医学影像学(电子版)2019，8，3： | 0 |
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