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| 1 | CRISPy-web: An online resource to design sgRNAs for CRISPR applications显示文摘CRISPR/Cas9-based genome editing has been one of the major achievements of molecular biology,allowing the targeted engineering of a wide range of genomes.The system originally evolved in prokaryotes as an adaptive immune system against bacteriophage infections.It now sees widespread application in genome engineering workflows,especially using the Streptococcus pyogenes endonuclease Cas9.To utilize Cas9,so-called single guide RNAs(sgRNAs)need to be designed for each target gene.While there are many tools available to design sgRNAs for the popular model organisms,only few tools that allow designing sgRNAs for non-model organisms exist.Here,we present CRISPy-web(http://gffzz2b22c00a04674870hfuf596o5f0on6v9n.ffgz.tsg.suse.edu.cn/),an easy to use web tool based on CRISPy to design sgRNAs for any userprovided microbial genome.CRISPy-web allows researchers to interactively select a region of their genome of interest to scan for possible sgRNAs.After checks for potential off-target matches,the resulting sgRNA sequences are displayed graphically and can be exported to text files.All steps and information are accessible from a web browser without the requirement to install and use command line scripts. | Kai Blin Lasse Ebdrup Pedersen TilmannWeber Sang Yup Lee | 2016 | Synthetic and Systems Biotechnology2016，1，2： | 3 |
| 2 | Renal cytokine profile in an endoto xemic porine model 显示文摘 | GRANFEDT A EBDRUP ITNNESEN E | 2008 | Acta Anaesthesiol scand2008，52，5： | 1 |
| 3 | Synthesis and biological and structural characterization of the dual- acting peroxisome proliferator-activated receptor alpha/gamma agonist ragaglitazar显示文摘 | Ebdrup S Pettersson I Rasmussen H B | 2003 | Journal of Medicinal Chemistry2003，46，8： | 1 |
| 4 | Synthesis and biological and structural characterization of the dualacting peroxisome proliferator-activated receptor α/γ agonist ragaglitazar显示文摘 | Ebdrup S Pettersson I Rasmussen HB | 2003 | J Med Chem2003，46，8： | 1 |
| 5 | Renal cytokine profile in all endotoxemie porcine model 显示文摘 | Granfeidt A Ebdrup L Tnnesen E | 2008 | Acta Anaesthesiol Scand2008，52，5： | 1 |
| 6 | Renal cytokine profile in an endotoxemic porcine model显示文摘 | GRANFEIDT A EBDRUP L TNNESEN E | 2008 | Acta Anaesthesiol Scand2008，52，5： | 1 |
| 7 | Synthesis and biologi- cal and structural characterization of the dual-acting peroxisome proliferator-activated receptor alpha/gamma agonist ragaglitazar 显示文摘 | Ebdrup S Pettersson I Rasmussen HB el al | 2003 | J Med Chem2003，46，8： | 1 |
| 8 | Synthesis and biologicall and structural characterization of the dual-acting peroxisome proliferator-activatest receptor α/γ agonist ragaglitazar显示文摘 | Ebdrup S Pettensson I Rasmussen HB | 2003 | J Med Chem2003，46，8： | 1 |
| 9 | Leukocyte, plasma, and organ-associated cytokine profiles in an animal model of acute inflammation显示文摘 | Ebdrup L Krog J Granfeldt A | 2008 | APMIS2008，116，5： | 1 |
| 10 | New 3-alkvlamino- 4H-thieno-1, 2, 4-thiadiazine 1, 1-dioxide derivatives activate ATP-sensitive potassium channels of pancreatic beta cells 显示文摘 | Nielsen F E Ebdrup S Jensen A F ct al | 2006 | J Med Chem2006，49，： | 1 |
| 11 | Leukocyte, plasma, and organ-associated cytokine profiles in an animal model of acute inflammation 显示文摘 | Ebdrup L Krog J Granfeldt A | 2008 | APMIS2008，116，5： | 1 |
| 12 | Synthesis and biological and structural characterization of the dual-acting peroxisome proliferator-activated receptor alpha/gamma agonist ragaglitazar显示文摘 | Ebdrup S Pettersson I Rasmussen HB | 2003 | J Med Chem2003，46，8： | 1 |
| 13 | Synthesis and biological and structural characterization of the dual2acting peroxisome proliferor activated receptor alpha/gamma agonist ragaglitazar显示文摘 | Ebdrup S Pettersson I Rasmussen H B | 2003 | Med Chem2003，46，8： | 1 |
| 14 | Synthesis and biological and structural characterization of the dual-acting peroxisome proliferator-activated receptor α/γ agonist ragaglitazar显示文摘 | EBDRUP S PETTERSSON I RASMUSSEN HB | 2003 | J Med Chem2003，46，8： | 1 |
| 15 | Synthesis and Structure-Activity Relationship for a Novel Class of PotentandSelective Carbamoyl-Triasole Based Inhibitors of Hor- mone SensitiveLipase 显示文摘 | Ebdrup S Sorensen L G Olsen 0 H | 2004 | J Med Chem2004，47，： | 1 |
| 16 | Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web‐based target finding tool显示文摘 | Carlotta Ronda Lasse Ebdrup Pedersen Henning Gram Hansen Thomas Beuchert Kallehauge Michael J. Betenbaugh Alex Toftgaard Nielsen Helene Faustrup Kildegaard | 2014 | Biotechnol. Bioeng2014，，： | 1 |
| 17 | Structureac- tivityrelationship for aryl and heteroaryl boronic acid inhib- itors othormone-sensitive lipase 显示文摘 | Ebdrup S Jacobsen P Farrington A D | 2005 | Bioorg Med Chem2005，13，： | 1 |
| 18 | Synthesis and bio- logical and structural characterizarion of the dual acting peroxisome proliferator activated cd~/agonist ragagl itazar显示文摘 | Ebdrup S Pettersson I Rasmussen H B | 2003 | J Med Chem2003，46，8： | 1 |
| 19 | Synthesis and Structure-Activity Relationship for a Novel Class of Po- tent and Selective Carbamate-Based Inhibitors of Hor- mone Selective Lipase with Acute In Vivo Antilipolytic Effects 显示文摘 | Ebdrup S Hanne H F R Christian F | 2007 | J Med Chem2007，50，22： | 1 |
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