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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Advantages of minimally- invasive reposition, retention, and Ilizarov-(hybrid)fixation for pilon-tibial-fractures fractures with particular emphasis on C2/C3 fractures 显示文摘 | Endres T Grass R Biewener A | 2004 | Unfallchirurg2004，107，4： | 1 |
| 3 | Renal glomerular lesions in unselected patients with cirrhosis undergoing orthotopic liver transplantation显示文摘 | Axelsen R A Crawford D H Endre Z H | | 0，，03： | 1 |
| 4 | Afro-assisted investigation of the corrosion behaviour of magnesium and Az91 alloys in an ionic liquid with varying water content显示文摘 | Shkurankov A E1 Abedin S Z Endres F | 2007 | Australian Journal of Chemistry2007，60，1： | 1 |
| 5 | Advantages of minimally-invasive reposition,retention,and Ilizarov-(hybrid) fixation for pilon-tibial-fractures with particular emphasis on C2/C3 fractures显示文摘 | Endres T Grass R Biewener A | 2004 | Unfallchirurg2004，107，4： | 1 |
| 6 | Environmental characteristics and bacterial counts in bedding and milk bulk tank of low-profile cross-ventilated, naturally ventilated, and compost bedded pack dairy barns显示文摘 | Lobeck K M Endres M I Janni K A | 2011 | Applied Engineering in Agriculture2011，28，1： | 1 |
| 7 | Adequacy of herniated disc tissue as a cell source for nucleus pulposus regeneration显示文摘 | Hegewald AA Endres M Abbushi A | 2011 | Neurosurg Spine2011，14，2： | 1 |
| 8 | Efficacy of tranexamic acid in reducing blood loss in posterior lumbar spine surgery for degenerative spinal stenosis with instability: a retrospective case control study 显示文摘 | Endres S Heinz M Wilke A | 2011 | BMC Surg2011，11，： | 1 |
| 9 | Risk factors for infection after spinal sugery显示文摘 | Fang A Hu S S Endres N | 2005 | Spine2005，30，12： | 1 |
| 10 | A receptor kinase regulating symbiotic nodule development显示文摘 | Endre G Kereszt A Kevei Z | 2002 | Nature2002，417，： | 1 |
| 11 | Instrumented intervertebral or pos- terolateral fusion in elderly patients clinical results of a single cen-ter 显示文摘 | Endres S Aigner R Wilke A | 2011 | B MC Musculoskelet Disord2011，12，1： | 1 |
| 12 | Modem theories of entrepreneurship behaviors: A comparison and appraisal 显示文摘 | Endres A M Woods C R | 2006 | Small Business Economics2006，26，2： | 1 |
| 13 | High resolution sediment and vegetation responses to Younger Dryas climate change in varved lake sediments from Meerfelder Maar,Germany显示文摘 | Brauer A Endres C G nter C Litt T Stebich M Negendank J F W | | 0，，： | 1 |
| 14 | A receptor kinase gene regulating symbiotic nodule development显示文摘 | Endre G Kereszt A Kevei Z | 2002 | Nature2002，417，6892： | 1 |
| 15 | Risk factors for infection after spinal surgery显示文摘 | FANG A HU S ENDRES N | 2005 | Spine2005，30，12： | 1 |
| 16 | Stroke protection by 3-hydroxy-3-methylglutaryl (HMG)- CoA reductase inhibitors mediated by endothelial ni- tric oxide synthase显示文摘 | ENDRES M LAUFS U HUANG A | 1998 | Proc Natl Acad Sei USA1998，95，： | 1 |
| 17 | HPV E6/E7 mRNA tran- scripts as predictors of high-grade epithelial cervix dysplasia显示文摘 | Mockel J Clad A Endres AS | 2007 | Diagn Pathol2007，2，1： | 1 |
| 18 | NO-Donors,part 3:nitrooxyacylated thiosalicylates and salicylates-synthesis and biological activities显示文摘 | Endres S Hacker A Noack E | 1999 | Eur J Med Chem1999，34，11： | 1 |
| 19 | Shedding of the amyloid precursor protein-like protein APLP2 by disintegvin-metalloproteinases 显示文摘 | Endres K Postina R Schroeder A | 2005 | FEBS J2005，272，22： | 1 |
| 20 | Leaf gas exchange,chloroplastic pigments and dry matter accumulation in castor bean (Ricinus communis L) seedlings subjected to salt stress conditions显示文摘 | PINHEIRO H A SILVA J V ENDRES L | | 0，，03： | 1 |
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