| 1 | Rapid Magnetic 3D Printing of Cellular Structures with MCF-7 Cell Inks显示文摘A contactless label-free method using a diamagnetophoretic ink to rapidly print three-dimensional(3D)scafold-free multicellular structures is described.Te inks consist of MCF-7 cells that are suspended in a culture medium to which a paramagnetic salt,diethylenetriaminepentaacetic acid gadolinium(III)dihydrogen salt hydrate(Gd-DTPA),is added.When a magnetic feld is applied,the host fuid containing the paramagnetic salt is attracted towards regions of high magnetic feld gradient,displacing the ink towards regions with a low gradient.Using this method,3D structures are printed on ultra-low attachment(ULA)surfaces.On a tissue culture treated(TCT)surface,a 3D printed spheroid coexists with a two-dimensional(2D)cell monolayer,where the composite is termed as a 2.5D structure.Te 3D structures can be magnetically printed within 6 hours in a medium containing 25 mM Gd-DTPA.Te infuence of the paramagnetic salt on MCF-7 cell viability,cell morphology,and ability of cells to adhere to each other to stabilize the printed structures on both ULA and TCT surfaces is investigated.Gene expressions of hypoxia-inducible factor 1-alpha(HIF1�)and vascular endothelial growth factor(VEGF)allow comparison of the relative stresses for the printed 3D and 2.5D cell geometries with those for 3D spheroids formed without magnetic assistance.Tis magnetic printing method can be potentially scaled to a higher throughput to rapidly print cells into 3D heterogeneous cell structures with variable geometries with repeatable dimensions for applications such as tissue engineering and tumour formation for drug discovery. | S.Mishriki A.R.Abdel Fattah T.Kammann R.P.Sahu F.Geng I.K.Puri | 2019 | Research2019，，1： | 2 |
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