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| 1 | Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204显示文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait. | Xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu Xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu Zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling | 2022 | Molecular Plant2022，15，9： | 6 |
| 2 | Highly active catalyst for vinyl acetate synthesis by modified activated carbon显示文摘从修改的激活的碳被准备的新锌醋酸盐催化剂向乙烯基醋酸盐的合成展出了极端活动。激活的碳被硝酸,讽刺和 peroxyacetic 酸(泛美航空公司) 修改。特定的区域,结构, pH 和由修正的搬运人的表面酸味组上的效果被讨论。羰基总计,在激活的碳的 carboxyl 组被 peroxyacetic 酸处理增加。新催化剂的生产率比用未经治疗的激活的碳准备的催化剂的高是 14.58% 。在羰基和 carboxyl 组(m) 和催化剂生产率(P) 的数量之间的关系是 P = 1.83 + 2.26 × 10 −3*e3.17m 。反应机制被建议。 | Chun Yan Hou Liang Rong Feng Fa Li Qiu | 2009 | Chinese Chemical Letters2009，20，7： | 5 |
| 3 | Yttria-stabilized-zirconia hollow spheres prepared by atmospheric plasma spray显示文摘Yttria-stabilizecl-zirconia(YSZ) hollow spheres are widely utilized for their novel physical and chemical properties.However,developing a simple and low-cost method for preparing such hollow spheres still remains a great challenge.In this paper,an atmospheric plasma spray(APS) method is introduced,and the formation mechanism of hollow 7YSZ(ZrO_2-7wt%Y_2O_3) spheres is presented.The hollow sphere morphology was observed by scanning electron microscopy(SEM) when agglomerated and sintered 7YSZ powders were used.Additionally,additive composition changes,phase transformations,and the thermal behavior of 7YSZ powders were analyzed by energy dispersive spectroscopy(EDS),X-ray diffractometry(XRD),thermogravimetric analysis(TG) and differential scanning calorimeter analysis(DSC).Furthermore,the phase transformations of agglomerated and sintered 7YSZ powders,7YSZ hollow spheres that annealed at various temperatures for different times are analyzed. | Xiaofeng Zhang Kesong Zhou Fa Chang Chen Song Chunming Deng Siyuan Liang | 2014 | Particuology2014，12，3： | 4 |
| 4 | Clinical practice guideline for transurethral plasmakinetic resection of prostate for benign prostatic hyperplasia(2021 Edition)显示文摘Benign prostatic hyperplasia (BPH) is highly prevalent among older men, impacting on their quality of life, sexual function, and genitourinary health, and has become an important global burden of disease. Transurethral plasmakinetic resection of prostate (TUPKP) is one of the foremost surgical procedures for the treatment of BPH. It has become well established in clinical practice with good efficacy and safety. In 2018, we issued the guideline “2018 Standard Edition”. However much new direct evidence has now emerged and this may change some of previous recommendations. The time is ripe to develop new evidence-based guidelines, so we formed a working group of clinical experts and methodologists. The steering group members posed 31 questions relevant to the management of TUPKP for BPH covering the following areas: questions relevant to the perioperative period (preoperative, intraoperative, and postoperative) of TUPKP in the treatment of BPH, postoperative complications and the level of surgeons’ surgical skill. We searched the literature for direct evidence on the management of TUPKP for BPH, and assessed its certainty generated recommendations using the grade criteria by the European Association of Urology. Recommendations were either strong or weak, or in the form of an ungraded consensus-based statement. Finally, we issued 36 statements. Among them, 23 carried strong recommendations, and 13 carried weak recommendations for the stated procedure. They covered questions relevant to the aforementioned three areas. The preoperative period for TUPKP in the treatment of BPH included indications and contraindications for TUPKP, precautions for preoperative preparation in patients with renal impairment and urinary tract infection due to urinary retention, and preoperative prophylactic use of antibiotics. Questions relevant to the intraoperative period incorporated surgical operation techniques and prevention and management of bladder explosion. The application to different populations incorporating the efficacy and safety of TUPKP in the treatment of normal volume (< 80 ml) and large-volume (≥ 80 ml) BPH compared with transurethral urethral resection prostate, transurethral plasmakinetic enucleation of prostate and open prostatectomy;the efficacy and safety of TUPKP in high-risk populations and among people taking anticoagulant (antithrombotic) drugs. Questions relevant to the postoperative period incorporated the time and speed of flushing, the time indwelling catheters are needed, principles of postoperative therapeutic use of antibiotics, follow-up time and follow-up content. Questions related to complications incorporated types of complications and their incidence, postoperative leukocyturia, the treatment measures for the perforation and extravasation of the capsule, transurethral resection syndrome, postoperative bleeding, urinary catheter blockage, bladder spasm, overactive bladder, urinary incontinence, urethral stricture, rectal injury during surgery, postoperative erectile dysfunction and retrograde ejaculation. Final questions were related to surgeons’ skills when performing TUPKP for the treatment of BPH. We hope these recommendations can help support healthcare workers caring for patients having TUPKP for the treatment of BPH. | Xian-Tao Zeng Ying-Hui Jin Tong-Zu Liu Fang-Ming Chen De-Gang Ding Meng Fu Xin-Quan Gu Bang-Min Han Xing Huang Zhi Hou Wan-Li Hu Xin-Li Kang Gong-Hui Li Jian-Xing Li Pei-Jun Li Chao-Zhao Liang Xiu-Heng Liu Zhi-Yu Liu Chun-Xiao Liu Jiu-Min Liu Guang-Heng Luo Yi Luo Wei-Jun Qin Jian-Hong Qiu Jian-Xin Qiu Xue-Jun Shang Ben-Kang Shi Fa Sun Guo-Xiang Tian Ye Tian Feng Wang Feng Wang Yin-Huai Wang Yu-Jie Wang Zhi-Ping Wang Zhong Wang Qiang Wei Min-Hui Xiao Wan-Hai Xu Fa-Xian Yi Chao-Yang Zhu Qian-Yuan Zhuang Li-Qun Zhou Xiao-Feng Zou Nian-Zeng Xing Da-Lin He Xing-Huan Wang 无 | 2022 | Military Medical Research2022，9，5： | 4 |
| 5 | NeuroBiometric:An Eye Blink Based Biometric Authentication System Using an Event-Based Neuromorphic Vision Sensor显示文摘The rise of the Internet and identity authentication systems has brought convenience to people's lives but has also introduced the potential risk of privacy leaks.Existing biometric authentication systems based on explicit and static features bear the risk of being attacked by mimicked data.This work proposes a highly efficient biometric authentication system based on transient eye blink signals that are precisely captured by a neuromorphic vision sensor with microsecond-level temporal resolution.The neuromorphic vision sensor only transmits the local pixel-level changes induced by the eye blinks when they occur,which leads to advantageous characteristics such as an ultra-low latency response.We first propose a set of effective biometric features describing the motion,speed,energy and frequency signal of eye blinks based on the microsecond temporal resolution of event densities.We then train the ensemble model and non-ensemble model with our Neuro Biometric dataset for biometrics authentication.The experiments show that our system is able to identify and verify the subjects with the ensemble model at an accuracy of 0.948 and with the non-ensemble model at an accuracy of 0.925.The low false positive rates(about 0.002)and the highly dynamic features are not only hard to reproduce but also avoid recording visible characteristics of a user's appearance.The proposed system sheds light on a new path towards safer authentication using neuromorphic vision sensors. | Guang Chen Fa Wang Xiaoding Yuan Zhijun Li Zichen Liang Alois Knoll | 2021 | IEEE/CAA Journal of Automatica Sinica2021，8，1： | 3 |
| 6 | Studies on fluorenscence resonance energy transfer between CdS nanoparticles and DOCAI dyes显示文摘The water-soluble CdS nanoparticles were synthesized in aqueous solution. A novel fluorescence resonance energy transfer (FRET) system with CdS nanoparticles as energy donors and 3,30-diethyl-oxadicarbocyanine iodide (DOCAI) dyes as energy accepter has been developed. | Lun Wang Yan Liu Hong Qi Chen A Ni Liang Fa Gong Xu | 2007 | Chinese Chemical Letters2007，18，3： | 2 |
| 7 | Emerging New Phylogenetic Groups of Rabies Virus in Chinese Ferret Badgers显示文摘Chinese ferret badger(FB)-transmitted rabies is a serious threat to public health in southeast China. Although mostly associated with dogs, the rabies virus(RABV) presents genetic diversity and has a significantly wide host range in China. Instead of the dog-and wildlife-associated China ⅠI lineage in the past decades, the China Ⅰ lineage has become the main epidemic group hosted and transmitted by dogs. In this study, four new lineages, including 43 RABVs from FBs, have been classified within the dog-dominated China Ⅰ lineage since 2014. FBRABVs have been previously categorized in the China Ⅱ lineage. Moreover, FB-hosted viruses seem to have become the main independent FB-associated clade in the phylogenetic tree. This claim suggests that the increasing genetic diversity of RABVs in FBs is a result of the selective pressure from coexisting dog rabies. FB transmission has become complicated and serious with the coexistence of dog rabies. Therefore, apart from targeting FB rabies, priority should be provided by the appropriate state agencies to perform mass immunization of dog against rabies. | MIAO Fa Ming CHEN Teng LIU Ye ZHANG Shou Feng ZHANG Fei LI Nan HU Rong Liang | 2018 | Biomedical and Environmental Sciences2018，31，6： | 2 |
| 8 | Epidemiological Survey of Hemoglobinopathies Based on Next-Generation Sequencing Platform in Hunan Province,China显示文摘Objective This study was aimed at investigating the carrier rate of,and molecular variation in,α-andβ-globin gene mutations in Hunan Province.Methods We recruited 25,946 individuals attending premarital screening from 42 districts and counties in all 14 cities of Hunan Province.Hematological screening was performed,and molecular parameters were assessed.Results The overall carrier rate of thalassemia was 7.1%,including 4.83%forα-thalassemia,2.15%forβ-thalassemia,and 0.12%for bothα-andβ-thalassemia.The highest carrier rate of thalassemia was in Yongzhou(14.57%).The most abundant genotype ofα-thalassemia andβ-thalassemia was-α^(3.7)/αα(50.23%)andβ^(IVS-Ⅱ-654)/β^(N)(28.23%),respectively.Fourα-globin mutations[CD108(ACC>AAC),CAP+29(G>C),Hb Agrinio and Hb Cervantes]and sixβ-globin mutations[CAP+8(C>T),IVS-Ⅱ-848(C>T),-56(G>C),beta nt-77(G>C),codon 20/21(-TGGA)and Hb Knossos]had not previously been identified in China.Furthermore,this study provides the first report of the carrier rates of abnormal hemoglobin variants andα-globin triplication in Hunan Province,which were 0.49%and 1.99%,respectively.Conclusion Our study demonstrates the high complexity and diversity of thalassemia gene mutations in the Hunan population.The results should facilitate genetic counselling and the prevention of severe thalassemia in this region. | XI Hui LIU Qin XIE Dong Hua ZHOU Xu TANG Wang Lan TANG De Guo ZENG Chun Yan WANG Qiong NIE Xing Hui PENG Jin Ping GAO Xiao Ya WU Hong Liang ZHANG Hao Qing QIU Li FENG Zong Hui WANG Shu Yuan ZHOU Shu Xiang HE Jun ZHOU Shi Hao ZHOU Fa Qun ZHENG Jun Qing WANG Shun Yao CHEN Shi Ping ZHENG Zhi Fen MA Xiao Yuan FANG Jun Qun LIANG Chang Biao WANG Hua | 2023 | Biomedical and Environmental Sciences2023，36，2： | 1 |
| 9 | Blue light induces mitochondrial DNA damage and free radical production in epithelial cells显示文摘 | Godley BF Shamsi FA Liang FQ | | 0，，： | 1 |
| 10 | Porous covalent organic frameworks-improved solid phase microextraction ambient mass spectrometry for ultrasensitive analysis of tetrabromobisphenol-A analogs显示文摘Owing to frequent environmental monitoring of tetrabromobisphenol-A(TBBPA)analogs and their potential ecotoxicological effects on organisms,analysis of trace levels of TBBPA analogs with more non-polar and less water-soluble characteristics is of great significance for studying their environmental behaviors and toxic effects.Herein,a fast and sensitive technique is developed for directly detecting aqueous TBBPA analogs,including TBBPA mono(allyl ether)(TBBPA-MAE),TBBPA mono(2,3-dibromopropyl ether)(TBBPAMDBPE),TBBPA mono(2-hydroxyethyl ether)(TBBPA-MHEE)and TBBPA mono(glycidyl ether)(TBBPAMGE),by combining solid phase microextraction(SPME)based on porous covalent organic frameworks(Porous-COFs)with constant flow desorption ionization-mass spectrometry(CFDI-MS).As chromatographic separation is replaced by constant flow desorption,each sample can be analyzed within 7 min.The hierarchical porous structures(microporous,mesoporous and macroporous)of COFs lead to the enhanced mass transfer and the easier accessibility of active sites to TBBPA analogs,so that the extraction efficiency is 2.3-3.6 times higher than pure microporous COFs,and far superior to commercial coatings.The detection limit and quantification limit of this method are 0.1-1 and 0.4-3.2 ng/L,respectively.Ultratrace levels of TBBPA analogs from 5.0 ng/L to 66 ng/L have been successfully detected in river and sea water samples,showing great potential for subsequent studies of their environmental behaviors and toxicological effects. | Wei Gao Min Li Yun Fa Zongshan Zhao Yaqi Cai Xiangfeng Liang Yongliang Yu Guibin Jiang | 2022 | Chinese Chemical Letters2022，33，8： | 1 |
| 11 | Numerical analysis of composite piled raft with cushion subjected to vertical load显示文摘 | Fa Yun Liang Long Zhu Chen Xu Guang Shi | 2003 | Computers and Geotechnics2003，30，： | 1 |
| 12 | Analysis of gene induction in human fibroblasts and bladder cancer cells exposed to the methylation inhibitor 5-aza-2'-deoxycytidine 显示文摘 | Gonzales FA Jones PA | 2002 | Cancer Res2002，62，4： | 1 |
| 13 | Feasibility of poplar foliages as bio-monitors for organochlorine pesticides in air显示文摘The feasibility of poplar (P. tomentosa) foliages as passive biomonitors for organochlorine pesticides in air was explored. The accumulation patterns of poplar foliages for HCHs, DDTs and HCB were similar, the amount of HCHs, DDTs and HCB increased with foliage growth in spring, and decreased thereafter. There was no obvious distinction in the accumulation styles between the adult leaf and the leaf-litter. This accumulation pattern is likely related to the growing process of the poplar foliage, and was ob-served for the first time in our work, giving an evidence for the 'bud burst effect' in plants. The tech-nical HCH and DDT were used largely in history and not used in recent years, but there was a little us-age of lindane and new input of o,p'-DDT in recent years, and dicofol usage may be the main source of o,p'-DDT. Concentrations of HCHs, DDTs and HCB in poplar foliages were similar to those in pine nee-dles at the corresponding period, and there is a positive strong correlation between OCP concentration data of two kinds of trees. It presents no difference in the accumulation style between two kinds of trees. The level of OCPs in the poplar foliage reflected the pollution status of OCPs in air. The result of this work showed that the poplar foliage can be used as the bio-monitor of OCPs in air. | DAI Tian You ZHU Xiao Hua WANG Wei MENG Wei YU YunJiang LI Fa Sheng YANG Yong Liang LIU Feng | 2009 | Chinese Science Bulletin2009，54，9： | 1 |
| 14 | An innovative model for the transient response of a spherical thin-shell transducer and an experimental confirmation显示文摘 | Lin Fa Xin Zhou YuXiao Fa YanDong Zhang JinPeng Mou Meng Liang MaoMao Wang Qi Zhang PengFei Ding WeiTing Feng Hong Yang MeiShan Zhao | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，11： | 1 |
| 15 | Numerical analysis of composite piled raft with cushion subjected to vertical load显示文摘 | Liang Fa Yun Chen Long Zhu Shi Xu Guang | 2003 | Computers and Geotechnics2003，30，6： | 1 |
| 16 | Blue light induces mitochondrial DNA damage and free radical production in epithelial ceils 显示文摘 | Godley BF Shamsi FA Liang FQ Jarrett SG Davies S Boulton M | 2005 | J Bio Chem2005，280，21： | 1 |
| 17 | Blue light induces mitochon-drial DNA damage and free radical production in epithelial cells显示文摘 | Godley BF Shamsi FA Liang FQ | 2005 | Biol Chem2005，280，21： | 1 |
| 18 | 用高度特异性王浆主蛋白1多克隆抗体ELISA法检测蜂王浆新鲜度(英文)显示文摘目的:为蜂王浆主蛋白1(MRJP1)的快速检测和鉴别提供科学依据,为蜂王浆的质量控制提供技术支持。创新点:首次比较了MRJP1特异性多克隆抗体与MRJP1重组表达蛋白多克隆抗体对王浆主蛋白家族的免疫反应差异,验证了蜂王浆中MRJP1蛋白降解与保温时间的相关性,建立了以MRJP1作为蜂王浆新鲜度生物标志物的快速检测方法。方法:通过蜂王浆主蛋白家族蛋白的氨基酸序列同源性分析,筛选出MRJP1的特异性多肽区域,进行人工合成,免疫兔子后取血清制备成特异性多克隆抗体。用蛋白质印迹法(Western blot)检测了MRJP1特异性多克隆抗体与MRJP1重组表达蛋白多克隆抗体对王浆主蛋白家族的免疫反应。以新鲜蜂王浆为对照品,用MRJP1特异性抗体酶联接免疫吸附剂测定(ELISA)法和变性电泳胶灰度扫描法分别测定保温(40°C)7-49天的蜂王浆中MRJP1含量的变化,并进行了相关性分析。结论:MRJP1的特异性抗体对MRJP1蛋白具有专一的免疫识别特性,可特异性地检测代表蜂王浆新鲜度的MRJP1含量变化,并鉴别蜂王浆的真伪。 | Li-rong SHEN Yi-ran WANG Liang ZHAI Wen-xiu ZHOU Liang-liang TAN Mei-lu LI Dan-dan LIU Fa XIAO | 2015 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2015，16，2： | 1 |
| 19 | A meta analysis of the effect of exercise training on left ventricular remodeling in heart failure patients:the benefit depends on the type of training performed显示文摘 | Haykowsky M J Liang Y Pechter D Jones LW McAlister FA Clark AM | | 0，，： | 1 |
| 20 | A variable deformation macadamia nut cracker显示文摘 | Tang GP Liang T Munchmeyer FA | 1982 | Transactions of the ASAE1982，25，6： | 1 |
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