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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | A genome-wide scan maps a novel juvenile-onset primary open angle glaucoma locus to chromosome 5q 显示文摘 | Pang CP Fan BJ Canlas O | 2006 | Mol Vis2006，12，： | 1 |
| 3 | Proteomics shows Hsp70 does not bind peptide sequences indiscriminately in vivo 显示文摘 | Grossmann ME Madden BJ Gao Fan | 2004 | Exp Cell Res2004，297，1： | 1 |
| 4 | Circulating ghrelin in pa-tients with gastric or colorectal cancer显示文摘 | Huang Q Fan YZ Ge BJ | 2007 | Dig Dis Sci2007，52，3： | 1 |
| 5 | Influence of the axotomy to cell body distance in rat rubrospinal and spinal motoneurons: differential regulation of GAP - 43, tubulins, and neurofilament-M 显示文摘 | Femandes KJ Fan DP Tsui BJ | 1999 | J Comp Neurol1999，414，4： | 1 |
| 6 | The tight junction pro-tein ZO-1 establishes a link between the transmembrane protein oc-cludin and the actin cytoskeleton显示文摘 | Fanning AS Jameson BJ Jesaitis LA | 1998 | Journal of Biological chemis-try1998，273，29： | 1 |
| 7 | Growth factor changes in Ex Vivo expansion of human limbal eithelial cells on human amniotic membrane显示文摘 | Yam HF Pang CP Fan DS Fan BJ Yu EY Lam DS | 2002 | Cornea2002，21，1： | 1 |
| 8 | An in vitro model of the back of the eye for studying retinal pigment epithelial-choroidal endothelial interactions显示文摘 | Fan W Zheng JJ McLaughlin BJ | 2002 | In Vitro Cell Dev Biol Anita2002，38，4： | 1 |
| 9 | The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton 显示文摘 | Fanning AS Jameson BJ Jesaitis LA | 1998 | J Biol Chem1998，273，29： | 1 |
| 10 | The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton 显示文摘 | Fanning AS Jameson BJ Jesaitis LA | 1998 | J Biol Chem1998，273，29： | 1 |
| 11 | The tight junction protein ZO-1 establishes a link between the transmembrane pro- tein occludin and the actin cytoskeleton 显示文摘 | Fanning AS Jameson BJ Jesaitis LA eta1 | 1998 | J Biol Chem1998，273，29： | 1 |
| 12 | Growth factor changes in vivo expansion of human limbal epithelial cells on human amniotic membrane显示文摘 | Yam HF Pang CP Fan DS Fan BJ Yu EY Lam DS | 2002 | Cornea2002，21，1： | 1 |
| 13 | LOXL1 promoterhaplotypes are associated with exfoliation syndrome in aUS Caucasian population显示文摘 | Fan BJ Pasquale LR Rhee D | 2011 | Invest Ophthalmol Vis Sci2011，525，： | 1 |
| 14 | The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton显示文摘 | Fanning AS Jameson BJ Jesaitis LA | | 0，，45： | 1 |
| 15 | Bone marrow mesenchymal stem cell from chronic hepatitis B patients differentiation into hepatocytelike cells显示文摘 | Fan BJ Piao YF Sun XY | | 0，，02： | 1 |
| 16 | The tight junction protein ZO-1 establishes a link between the transmembrane pro- tein occludin and the actin cytoskeleton 显示文摘 | Fanning AS Jameson BJ Jesaitis LA eta1 | 1998 | J Biol Chem1998，273，29： | 1 |
| 17 | Gene mapping for primary open angle glaucoma显示文摘 | Fan BJ Wang DY Lam DSC Pang CP | 2006 | Clin Biochem2006，39，3： | 1 |
| 18 | The tight junction protein ZO-1 establishes link between the transmembrane protein occludin and the actin cytoskeleton 显示文摘 | Fanning AS Jameson BJ Jesaitis LA | 1998 | JBiol Chem1998，273，29： | 1 |
| 19 | The tight junction protein ZO-1 establishes a link between the transmembrane protein oeeludin and the aetin eytoskeleton显示文摘 | Fanning AS Jameson BJ Jesaitis LA | 1998 | J Biol Chem1998，273，29： | 1 |
| 20 | The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton显示文摘 | Fanning AS Jameson BJ Jesaitis LA | 1998 | J BioI Chern1998，273，29： | 1 |
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