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| 1 | Os isotope dating and growth hiatuses of Co-rich crust from central Pacific显示文摘Up to now, accurate determination of the growth age and hiatuses of the Co-rich crust is still a difficult work, which constrains the researches on the genesis, growth process, controlling factors, regional tectonics, paleo-oceanographic background, etc. of the Co-rich crust. This paper describes our work in determining the initial growth age of the Co-rich crust to be of the late Cretaceous Campanian Stage (about 75-80 Ma), by selecting the Co-rich crust with clear multi-layer structures in a central Pacific seamount for layer-by-layer sample analysis and using a number of chronological methods, such as Co flux dating, dating by correlation with 187Os/188Os evolution curves of seawater, and stratigraphic divi- sion by calcareous nannofossils. We have also discovered growth hiatuses with different time intervals in the early Paleocene, middle Eocene, late Eocene and early-middle Miocene, respectively. These re- sults have provided an important age background for further researches on the Co-rich crust growth process and the paleo-oceanographic environment evolution thereby revealed in the said region. | LI JiangShan FANG NianQiao QU WenJun DING Xuan GAO LianFeng WU ChangHang ZHANG ZhenGuo | 2008 | Science China Earth Sciences2008，51，10： | 6 |
| 2 | Allele-aware chromosome-scale assembly of the allopolyploid genome of hexaploid Ma bamboo(Dendrocalamus latiflorus Munro)显示文摘Dendrocalamus latiflorus Munro is a woody clumping bamboo with rapid shoot growth.Both genetic transformation and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene editing techniques are available for D.latiflorus,enabling reverse genetic approaches.Thus,D.latiflorus has the potential to be a model bamboo species.However,the genome sequence of D.latiflorus has remained unreported due to its polyploidy and large genome size.Here,we sequenced the D.latiflorus genome and assembled it into three allele-aware subgenomes(AABBCC),representingthe largest genome of a major bamboo species.We assembled 70 allelic chromosomes(2,737 Mb)for hexaploid D.latiflorus using both singlemolecule sequencing from the Pacific Biosciences(Pac Bio)Sequel platform and chromosome conformation capture sequencing(Hi-C).Repetitive sequences comprised 52.65%of the D.latiflorus genome.We annotated 135231 protein-coding genes in the genome based on transcriptomes from eight different tissues.Transcriptome sequencing using RNA-Seq and Pac Bio singlemolecule real-time long-read isoform sequencing revealed highly differential alternative splicing(AS)between non-abortive and abortive shoots,suggesting that AS regulates the abortion rate of bamboo shoots.This high-quality hexaploid genome and comprehensive strand-specific transcriptome datasets for this Poaceae family member will pave the way for bamboo research using D.latiflorus as a model species. | Yushan Zheng Deming Yang Jundong Rong Liguang Chen Qiang Zhu Tianyou He Lingyan Chen Jing Ye Lili Fan Yubang Gao Hangxiao Zhang Lianfeng Gu | 2022 | Journal of Integrative Plant Biology2022，64，3： | 6 |
| 3 | TRDMT1 exhibited protective effects against LPS-induced inflammation in rats through TLR4-NF-κB/MAPK-TNF-αpathway显示文摘Background:Inflammation is a complex physiological and pathological process.Although many types of inflammation are well characterized,their physiological func-tions are largely unknown.tRNA aspartic acid methyltransferase 1(TRDMT1)has been implicated as a stress-related protein,but its intrinsic biological role is unclear.Methods:We constructed a Trdmt1 knockout rat and adopted the LPS-induced sepsis model.Survival curve,histopathological examination,expression of inflammatory fac-tors,and protein level of TLR4 pathway were analyzed.Results:Trdmt1 deletion had no obvious impact on development and growth.Trdmt1 de-letion slightly increased the mortality during aging.Our data showed that Trdmt1 strongly responded in LPS-treated rats,and Trdmt1 knockout rats were vulnerable to LPS treat-ment with declined survival rate.We also observed more aggravated tissue damage and more cumulative functional cell degeneration in LPS-treated knockout rats compared with control rats.Further studies showed upregulated TNF-αlevel in liver,spleen,lung,and serum tissues,which may be explained by enhanced p65 and p38 phosphorylation.Conclusions:Our data demonstrated that Trdmt1 plays a protective role in inflamma-tion by regulating the TLR4-NF-κB/MAPK-TNF-αpathway.This work provides useful information to understand the TRDMT1 function in inflammation. | Zhengguang Li Xiaolong Qi Xu Zhang Lei Yu Lijuan Gao Weining Kong Wei Chen Wei Dong Lijun Luo Dan Lu Lianfeng Zhang Yuanwu Ma | 2022 | Animal Models and Experimental Medicine2022，5，2： | 5 |
| 4 | SARS-CoV-2 infection aggravates chronic comorbidities of cardiovascular diseases and diabetes in mice显示文摘Background:Cardiovascular diseases(CVDs)and diabetes mellitus(DM)are top two chronic comorbidities that increase the severity and mortality of COVID-19.However,how SARS-CoV-2 alters the progression of chronic diseases remain unclear.Methods:We used adenovirus to deliver h-ACE2 to lung to enable SARS-CoV-2 infection in mice.SARS-CoV-2’s impacts on pathogenesis of chronic diseases were studied through histopathological,virologic and molecular biology analysis.Results:Pre-existing CVDs resulted in viral invasion,ROS elevation and activation of apoptosis pathways contribute myocardial injury during SARS-CoV-2 infection.Viral infection increased fasting blood glucose and reduced insulin response in DM model.Bone mineral density decreased shortly after infection,which associated with impaired PI3K/AKT/mTOR signaling.Conclusion:We established mouse models mimicked the complex pathological symptoms of COVID-19 patients with chronic diseases.Pre-existing diseases could impair the inflammatory responses to SARS-CoV-2 infection,which further aggravated the pre-existing diseases.This work provided valuable information to better understand the interplay between the primary diseases and SARS-CoV-2 infection. | Yuanwu Ma Dan Lu Linlin Bao Yajin Qu Jiangning Liu Xiaolong Qi Lei Yu Xu Zhang Feifei Qi Qi Lv Yunpeng Liu Xudong Shi Caixian Sun Jing Li Jie Wang Yunlin Han Kai Gao Wei Dong Ning Liu Shan Gao Jing Xue Qiang Wei Sidan Pan Hong Gao Lianfeng Zhang Chuan Qin | 2021 | Animal Models and Experimental Medicine2021，4，1： | 3 |
| 5 | Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats显示文摘Background:The GGGGCC(G4C2)repeat expansion in the human open reading frame 72 on chromosome 9,C9orf72,is the most common cause of amyotrophic lateral sclerosis(ALS).Studies in transgenic mouse models have linked the pathogenic mechanism of G4C2 repeat expansion to RNA foci or the accumulation of unnatural dipeptide repeats in neurons.However,only one of the existing transgenic mouse lines developed typical ALS.Methods:C9orf72 knockin rats were generated by knockin of 80 G4C2 repeats with human flanking fragments within exon1a and exon1b at the rat C9orf72 locus.Protein expression was detected by western blot.Motor coordination and grip force were measured using a Rotarod test and a grip strength test.Neurodegeneration was assessed by Nissl staining with cresyl violet.Results:C9orf72 haploinsufficiency reduced C9orf72 protein expression 40%in the cerebrum,cerebellum and spinal cords from knockin rats(P<.05).The knockin(KI)rats developed motor deficits from 4 months of age.Their falling latencies and grip force were decreased by 67%(P<.01)and 44%(P<.01),respectively,at 12 months of age compared to wild-type(WT)mice.The knockin of the hexanucleotide repeat expansion(HRE)caused a 47%loss of motor neurons in the spinal cord(P<.001)and 25%(5/20)of female KI rats developed hind limb paralysis at 13 to 24 months.Conclusion:Motor defects in KI rats may result from neurotoxicity caused by HRE and the resulting reduction in C9orf72 protein due to haploinsufficiency.These KI rats could be a useful model for investigating the contributions of loss-of-function to neurotoxicity in C9orf72-related ALS. | Wei Dong Li Zhang Caixian Sun Xiang Gao Feifei Guan Jing Li Wei Chen Yuanwu Ma Lianfeng Zhang | 2020 | Animal Models and Experimental Medicine2020，3，3： | 2 |
| 6 | The relationship between the growth process of the ferromanganese crusts in the Pacific seamount and Cenozoic ocean evolvement显示文摘Base on the Os isotope stratigraphy together with the empirical growth rate models using Co concentrations, the growth ages of the ferromanganese crusts MHD79 and MP3D10 distributed in the seamount of Pacific are confirmed. Through the contrast and research on the previous achievements including ODP Leg 144 and the crusts CD29-2, N5E-06 and N1-15 of the seamount of the Central Pacific, the uniform five growth and growth hiatus periods of them are found, and closely related to the Cenozoic ocean evolvement process. In the Paleocene Carbon Isotope Maximum (PCIM), the rise of the global ocean productivity promoted the growth of the seamount crust; the first growth hiatus (I) of the ferromanganese crust finished. In the Paleocene-Eocene Thermal Maximum (PETM), though the vertical exchange of seawater was weakened, the strong terrestrial chemical weathering led to the input of a great amount of the terrigenous nutrients, which made the bioproductivity rise, so there were no crust hiatuses. During 52-50 Ma, the Early Eocene Optimum Climate (EECO), the two poles were warm, the latitudinal temperature gradient was small, the wind-driven sea circulation and upwelling activity were weak, the terrestrial weathering was also weakened, the open ocean bioproductivity decreased, and the ferromanganese crust had growth hiatus again (II). From early Middle Eocene-Late Eocene, Oligocene, it was a long-term gradually cooling process, the strengthening of the sea circulation and upwelling led to a rise of bioproductivity, and increase of the content of the hydrogenous element Fe, Mn and Co and the biogenous element Cu, Zn, so that was the most favorable stage for the growth of ferromanganese crust (growth periods III and IV) in the studied area. The hiatus III corresponded with the Eocene-Oligocene boundary, is inferred to relate with the global climate transformation, celestial body impact event in the Eocene-Oligocene transition. From the early to the middle Miocene, a large-scale growth hiatus (hiatus period IV) of the ferromanganese crust in the studied area is inferred to relate with temporary warm up climate and ephemeral withdrawal of Antarctic bottom water in the early Miocene. After that, the Antarctic ice sheets extended, the bottom water circumfluence strengthened, the ocean fertility increased, and the once interrupted crust continued to grow in the late Miocene (growth period V). | DING Xuan GAO LianFeng FANG NianQiao QU WenJun LIU Jian LI JiangShan | 2009 | Science China Earth Sciences2009，52，8： | 2 |
| 7 | Novel rat model of multiple mitochondrial dysfunction syndromes(MMDS)complicated with cardiomyopathy显示文摘Background:Multiple mitochondrial dysfunction syndromes(MMDS)presents as complex mitochondrial damage,thus impairing a variety of metabolic pathways.Heart dysplasia has been reported in MMDS patients;however,the specific clinical symptoms and pathogenesis remain unclear.More urgently,there is a lack of an animal model to aid research.Therefore,we selected a reported MMDS causal gene,Isca1,and established an animal model of MMDS complicated with cardiac dysplasia.Methods:The myocardium-specific Isca1 knockout heterozygote(Isca1 HET)rat was obtained by crossing the Isca1 conditional knockout(Isca1 cKO)rat with theαmyosin heavy chain Cre(α-MHC-Cre)rat.Cardiac development characteristics were determined by ECG,blood pressure measurement,echocardiography and histopatho-logical analysis.The responsiveness to pathological stimuli were observed through adriamycin treatment.Mitochondria and metabolism disorder were determined by activity analysis of mitochondrial respiratory chain complex and ATP production in myocardium.Results:ISCA1 expression in myocardium exhibited a semizygous effect.Isca1 HET rats exhibited dilated cardiomyopathy characteristics,including thin-walled ventri-cles,larger chambers,cardiac dysfunction and myocardium fibrosis.Downregulated ISCA1 led to deteriorating cardiac pathological processes at the global and organiza-tional levels.Meanwhile,HET rats exhibited typical MMDS characteristics,including damaged mitochondrial morphology and enzyme activity for mitochondrial respira-tory chain complexesⅠ,ⅡandⅣ,and impaired ATP production.Conclusion:We have established a rat model of MMDS complicated with cardiomyopathy,it can also be used as model of myocardial energy metabolism dysfunction and mitochondrial cardiomyopathy.This model can be applied to the study of the mechanism of energy metabolism in cardiovascular diseases,as well as research and development of drugs. | Yahao Ling Jiaxin Ma Xiaolong Qi Xu Zhang Qi Kong Feifei Guan Wei Dong Wei Chen Shan Gao Xiang Gao Shuo Pan Yuanwu Ma Dan Lu Lianfeng Zhang | 2021 | Animal Models and Experimental Medicine2021，4，4： | 2 |
| 8 | Effect of Different Cultivation Practices on Soil Physical and Chemical Properties for Greenhouse Vegetables under Long-Term Continuous Cropping显示文摘Five kinds of greenhouse vegetables( eggplant,loofah,tomato,cucumber and pepper) were selected in summer uprooting stage from greenhouse in Shouguang area,Shandong Province. Total nitrogen,ammonium nitrogen,nitrate nitrogen,available phosphorus,available potassium,organic matter content and p H,EC value of three soil layers were measured,respectively. The results showed that the total nitrogen,ammonium nitrogen,nitrate nitrogen,available phosphorus and available potassium in the soil were mainly accumulated in the upper soil,which made the soil acidification trend,because different farmers adopted different cultivation practices for different greenhouse vegetables in Shouguang region,but there was no significant effect on soil EC value. The input of ammonium nitrogen and nitrate nitrogen and other related fertilizers to greenhouse cucumber was higher than that of other greenhouse vegetables,but the amount of available potassium fertilizer and organic matter input to greenhouse tomato under different cultivation practices was lower than other greenhouse vegetables. In Shouguang area,the input of organic matter into greenhouse vegetables should be increased by increasing the proportion of manure input to increase the content of soil organic carbon,so as to achieve the balance of carbon and nitrogen ratio,and to provide a scientific basis for the establishment of an evaluation system for the environmental benefits brought about by chemical fertilizer reduction in greenhouse vegetables in Shouguang area. | Lijuan GAO Guoyuan ZOU Lianfeng DU Shunjiang LI Jing LIU Hongkai DUAN | 2018 | Asian Agricultural Research2018，10，9： | 2 |
| 9 | Evodiamine derivatives improve cognitive abilities in APP^(swe)/PS1^(ΔE9) transgenic mouse models of Alzheimer's disease显示文摘Background: Alzheimer's disease(AD) is a complex neurodegenerative disease. Due to the complexity of its molecular pathogenesis and the interaction of the numerous factors involved, the etiology and pathogenesis of AD have not been fully elucidated. Therefore, effective treatment for AD remains to be developed. Evodiamine, a quinolone alkaloid, has been found to improve learning and memory ability to in the APP^(swe)/PS1^(ΔE9) mouse model of dementia. However, the cytotoxicity and physicochemical properties of evodiamine have limited its use in the treatment of AD.Methods: Evodiamine and its derivatives were effectively synthesized by EDCImediated condensation at room temperature. These target compounds contained 1 thio-and 21 oxo-evodiamine derivatives with different substituted groups. The cytotoxicity of evodiamine and its derivatives and the neuroprotective effects of the evodiamine derivatives against H_2O_2-induced cell loss in SH-SY5 Y cells were investigated using the WST-8 assay. The Morris water-maze test was used to detect the effect of evodiamine and its derivatives on improving learning and memory in APP^(swe)/PS1^(ΔE9) mice.Results: In this study, a series of oxo-and thio-evodiamine derivatives was synthesized. Several derivatives showed lower cytotoxicity and stronger neuroprotective effects than evodiamine and elicited enhanced cognitive improvement, especially in the test of spatial memory in APP^(swe)/PS1^(ΔE9) mice.Conclusion: Our study provides insights for developing novel evodiamine derivatives for chemical intervention and treatment of AD. | Shuo Pang Caixian Sun Shan Gao Yajun Yang Xiandao Pan Lianfeng Zhang | 2020 | Animal Models and Experimental Medicine2020，3，2： | 2 |
| 10 | Heterotrophy-coordinated diazotrophy is associated with significant changes of rare taxa in soil microbiome显示文摘Soil heterotrophic respiration during decomposition of carbon(C)-rich organic matter plays a vital role in sustaining soil fertility.However,it remains poorly understood whether dinitrogen(N_(2))fixation occurs in support of soil heterotrophic respiration.In this study,^(15)N_(2)-tracing indicated that strong N_(2)fixation occurred during heterotrophic respiration of carbon-rich glucose.Soil organic ^(15)N increased from 0.37 atom%to 2.50 atom%under aerobic conditions and to 4.23 atom%under anaerobic conditions,while the concomitant CO_(2)flux increased by 12.0-fold under aerobic conditions and 5.18-fold under anaerobic conditions.Soil N_(2)fixation was completely absent in soils replete with inorganic N,although soil N bioavailability did not alter soil respiration.High-throughput sequencing of the 16S rRNA gene further indicated that:i)under aerobic conditions,only 15.2%of soil microbiome responded positively to glucose addition,and these responses were significantly associated with soil respiration and N_(2)fixation and ii)under anaerobic conditions,the percentage of responses was even lower at 5.70%.Intriguingly,more than 95%of these responses were originally rare with<0.5%relative abundance in background soils,including typical N_(2)-fixing heterotrophs such as Azotobacter and Clostridium and well-recognized non-N_(2)-fixing heterotrophs such as Sporosarcina,Agromyces,and Sedimentibacter.These results suggest that only a small portion of the soil microbiome could respond quickly to the amendment of readily accessible organic C in a fluvo-aquic soil and highlighted that rare phylotypes might have played more important roles than previously appreciated in catalyzing soil C and nitrogen turnovers.Our study indicates that N_(2)fixation could be closely associated with microbial turnover of soil organic C when available in excess. | Wei GAO Lianfeng WANG Zhongjun JIA | 2022 | Pedosphere2022，32，3： | 1 |
| 11 | Genome-wide profiling of circular RNAs,alternative splicing,and R-loops in stem-differentiating xylem of Populus trichocarpa显示文摘Circular RNAs(circRNAs)are a recently dis-covered type of non‐coding RNA derived from pre‐mRNAs.R‐loops consist of a DNA:RNA hybrid andthe associated single‐stranded DNA.InArabi-dopsis thaliana,circRNA:DNA R‐loops regulatealternative splicing(AS)ofSEPALLATA3(SEP3).However,the occurrence and functions ofcircRNAs and R‐loops inPopulus trichocarpaarelargely unexplored.Here,we performed circRNA‐enriched sequencing in the stem‐differentiatingxylem(SDX)ofP.trichocarpaand identified 2,742distinct circRNAs,including circ‐CESA4,circ‐IRX7,and circ‐GUX1,which are generated from genesinvolved in cellulose,and hemicellulose biosyn-thesis,respectively.To investigate the roles ofcircRNAs in modulating alternative splicing(AS),we detected 7,836 AS events using PacBio Iso‐Seq and identified 634 circRNAs that overlappedwith 699 AS events.Furthermore,using DNA:RNAhybrid immunoprecipitation followed by se-quencing(DRIP‐seq),we identified 8,932 R‐looppeaks that overlapped with 181 circRNAs and 672AS events.Notably,several SDX‐related circRNAsoverlapped with R‐loop peaks,pointing to theirpossible roles in modulating AS in SDX.Indeed,overexpressing circ‐IRX7increased the levels ofR‐loop structures and decreased the frequency ofintron retention in linearIRX7transcripts.Thisstudy provides a valuable R‐loop atlas resourceand uncovers the interplay between circRNAs andAS in SDX ofP.trichocarpa. | Xuqing Liu Yubang Gao Jiakai Liao Miao Miao Kai Chen Feihu Xi Wentao Wei Huihui Wang Yongsheng Wang XiXu Anireddy S.N.Reddy Lianfeng Gu | 2021 | Journal of Integrative Plant Biology2021，63，7： | 1 |
| 12 | Multi-omics of Circular RNAs and Their Responses to Hormones in Moso Bamboo(Phyllostachys edulis)显示文摘Circular RNAs(circRNAs)are endogenous non-coding RNAs with covalently closed structures,which have important functions in plants.However,their biogenesis,degradation,and function upon treatment with gibberellins(GAs)and auxins(1-naphthaleneacetic acid,NAA)remain unknown.Here,we systematically identified and characterized the expression patterns,evolutionary conservation,genomic features,and internal structures of circRNAs using RNase R-treated libraries from moso bamboo(Phyllostachys edulis)seedlings.Moreover,we investigated the biogenesis of circRNAs dependent on both cis-and trans-regulation.We explored the function of circRNAs,including their roles in regulating microRNA(miRNA)-related genes and modulating the alternative splicing of their linear counterparts.Importantly,we developed a customized degradome sequencing approach to detect miRNA-mediated cleavage of circRNAs.Finally,we presented a comprehensive view of the participation of circRNAs in the regulation of hormone metabolism upon treatment of bamboo seedlings with GA and NAA.Collectively,our study provides insights into the biogenesis,function,and miRNA-mediated degradation of circRNAs in moso bamboo. | Yongsheng Wang Huihui Wang Huiyuan Wang Ruifan Zhou Ji Wu Zekun Zhang Yandong Jin Tao Li Markus V.Kohnen Xuqing Liu Wentao Wei Kai Chen Yubang Gao Jiazhi Ding Hangxiao Zhang Bo Liu Chentao Lin Lianfeng Gu | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 1 |
| 13 | Long-term naringin consumption reverses a glucose uptake defect and improves cognitive deficits in a mouse model of Alzheimer’s disease显示文摘 | Dongmei Wang Kai Gao Xiaoying Li Xinhua Shen Xu Zhang Chunmei Ma Chuan Qin Lianfeng Zhang | 2012 | Pharmacology, Biochemistry and Behavior2012，，1： | 1 |
| 14 | Knockout of ISCA 1 causes early embryonic death in rats显示文摘Background : Iron-sulfur cluster assembly 1( ISCA 1) is an iron-sulfur(Fe/S) carrier protein that accepts Fe/S from a scaffold protein and transfers it to target proteins including the mitochondrial Fe/S containing proteins. ISCA 1 is also the newly identified causal gene for multiple mitochondrial dysfunctions syndrome( MMDS). However, our knowledge about the physiological function of ISCA 1 in vivo is currently limited. In this study, we generated an ISCA 1 knockout rat line and analyzed the embryo development. Methods : ISCA 1 knockout rats were generated by replacing the exon1 of ISCA 1 gene with the mC herry-Cre fusion gene using CRISPR-Cas9 technology. The ISCA 1 expression pattern was analyzed by fluorescence imaging using ISCA 1 promotor driven Cre and mC herry expression. The embryonic morphology was examinated by microscope and mitochondrial proteins were tested by Western blot. Results : An ISCA 1 knockout rat line was obtained, which expressed mC herry-Cre fusion protein. Both of the fluorescence images from mC herry and Cre induced mC herry in a reporter rat strain, showing that ISCA 1 expressed in most of the tissues in rats. The ISCA 1 knockout resulted in abnormal development at 8.5 days, with a significant decrease of NDUFA 9 protein and an increase of aconitase 2( ACO 2) in rat embryos. Conclusion : Deletion of ISCA 1 induced early death in rats. ISCA 1 affected the expression of key proteins in the mitochondrial respiratory chain complex, suggesting that ISCA 1 has an important influence on the respiratory complex and energy metabolism. | Xinlan Yang Dan Lu Xu Zhang Wei Chen Shan Gao Wei Dong Yuanwu Ma Lianfeng Zhang | 2019 | Animal Models and Experimental Medicine2019，2，1： | 1 |
| 15 | Transgenic expression of IL-33 activates CD8 + T cells and NK cells and inhibits tumor growth and metastasis in mice显示文摘 | Kun Gao Xiaoying Li Li Zhang Lin Bai Wei Dong Kai Gao Guiying Shi Xianzhu Xia Lingying Wu Lianfeng Zhang | 2013 | Cancer Letters2013，，2： | 1 |
| 16 | The nuclear cytokine IL-37a controls lethal cytokine storms primarily via IL-1R8-independent transcriptional upregulation of PPARγ显示文摘Cytokine storms are crucial in the development of various inflammatory diseases,including sepsis and autoimmune disorders.The immunosuppressive cytokine INTERLEUKIN(IL)-37 consists of five isoforms(IL-37a-e).We identified IL-37a as a nuclear cytokine for the first time.Compared to IL-37b,IL-37a demonstrated greater efficacy in protecting against Toll-like receptor-induced cytokine hypersecretion and lethal endotoxic shock.The full-length(FL)form of IL-37a and the N-terminal fragment,which is processed by elastase,could translocate into cell nuclei through a distinctive nuclear localization sequence(NLS)/importin nuclear transport pathway.These forms exerted their regulatory effects independent of the IL-1R8 receptor by transcriptionally upregulating the nuclear receptor peroxisome proliferator-activated receptor(PPARγ).This process involved the recruitment of the H3K4 methyltransferase complex WDR5/MLL4/C/EBPβand H3K4me1/2 to the enhancer/promoter of Pparg.The receptor-independent regulatory pathway of the nuclear IL-37a–PPARγaxis and receptor-dependent signaling by secreted IL-37a maintain homeostasis and are potential therapeutic targets for various inflammatory diseases,including sepsis. | Rongfei Wei Xiao Han Mengyuan Li Yuan Ji Lianfeng Zhang Maria-Ioanna Christodoulou Najwa Jameel Hameed Aga Caiyan Zhang Ran Gao Jiangning Liu Jinrong Fu Guoping Lu Xiaojun Xiao Xiaoyu Liu Ping-Chang Yang Iain B.McInnes Ying Sun Peisong Gao Chuan Qin Shau-Ku Huang Yufeng Zhou Damo Xu | 2023 | Cellular & Molecular Immunology2023，20，12： | 0 |
| 17 | Hydrothermal synthesis and characterization of MnCo_(2)O_(4) in the low-temperature hydrothermal process:Their magnetism and electrochemical properties显示文摘MnCo_(2)O_(4) octahedral structure with edge length about 500 nm was successfully synthesized by a simple hydrothermal route.With the use of NaOH,the chemical potential and the rate of ionic motion in the precursor solution were controlled,and the particle size was limited.The magnetization measurements revealed that the products exhibited ferrimagnetic characteristics with different saturation magnetization and coercivity at different measuring temperatures.In addition,the as-prepared MnCo_(2)O_(4) as anodes for lithium-ion batteries(LIBs)exhibited a reversible capacity of 1180 mA·h/g and 1090 mA·h/g at current density of 0.1 C and 1 C,respectively.The excellent cyclic performance was confirmed because the value of reversible capacity for MnCo_(2)O_(4) was 618 mA·h/g after 50 cycles at 0.1 C.Owing to the good rate performance,MnCo_(2)O_(4) octahedral products were suggested to have a promising application as anode material for LIBs. | Lianfeng DUAN Fenghui GAO Limin WANG Songzhe JIN Hua WU | 2013 | Journal of Advanced Ceramics2013，2，3： | 0 |
| 18 | A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes显示文摘Background:Multiple mitochondrial dysfunction syndromes(MMDS)are rare mitochondrial diseases caused by mutation of mitochondrial iron–sulfur cluster synthesis proteins.This study established a rat model simulating MMDS5 disease in the nervous system to investigate its pathological features and neuronal death.Methods:We generated neuron-specific Isca1 knockout rat(Isca1 flox/flox-NeuN-Cre)using CRISPR-Cas9 technology.The brain structure changes of CKO rats were studied with MRI,and the behavior abnormalities were analyzed through gait analysis and open field tests,Y maze tests and food maze tests.The pathological changes of neurons were analyzed through H&E staining,Nissl staining,and Golgi staining.Mitochondrial damage was assessed by TEM,western blot and ATP assay,and the morphology of neurons was assessed by WGA immunofluorescence to detect the death of neurons.Results:This study established the disease model of MMDS5 in the nervous system for the first time,and found that after Isca1 loss,the rats suffered from developmental retardation,epilepsy,memory impairment,massive neuronal death,reduced number of Nissl bodies and dendritic spines,mitochondrial fragmentation,cristae fracture,reduced content of respiratory chain complex protein,and reduced production of ATP.Isca1 knockout caused neuronal oncosis.Conclusions:This rat model can be used to study the pathogenesis of MMDS.In addition,compared with human MMDS5,the rat model can survive up to 8 weeks of age,effectively extending the window of clinical treatment research,and can be used for the treatment of neurological symptoms in other mitochondrial diseases. | Hanxuan Sheng Dan Lu Xiaolong Qi Yahao Ling Jing Li Xu Zhang Wei Dong Wei Chen Shan Gao Xiang Gao Li Zhang Lianfeng Zhang | 2023 | Animal Models and Experimental Medicine2023，6，2： | 0 |
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