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| 1 | Chronic stress sensitizes rats to pancreatitis induced by cerulein:Role of TNF-α显示文摘AIM:To investigate chronic stress as a susceptibility factor for developing pancreatitis,as well as tumor necrosis factor-α (TNF-α) as a putative sensitizer.METHODS:Rat pancreatic acini were used to analyze the influence of TNF-α on submaximal (50 pmol/L) cholecystokinin (CCK) stimulation.Chronic restraint (4 h every day for 21 d) was used to evaluate the effects of submaximal (0.2 μg/kg per hour) cerulein stimulation on chronically stressed rats.RESULTS:In vitro exposure of pancreatic acini toTNF-α disorganized the actin cytoskeleton.This was further increased by TNF-α/CCK treatment,which additionally reduced amylase secretion,and increased trypsin and nuclear factor-κB activities in a protein-kinase-C δ and ε-dependent manner.TNF-α/CCK also enhanced caspases' activity and lactate dehydrogenase release,induced ATP loss,and augmented the ADP/ATP ratio.In vivo,rats under chronic restraint exhibited elevated serum and pancreatic TNF-α levels.Serum,pancreatic,and lung inflammatory parameters,as well as caspases' activity in pancreatic and lung tissue,were substantially enhanced in stressed/cerulein-treated rats,which also experienced tissues' ATP loss and greater ADP/ATP ratios.Histological examination revealed that stressed/cerulein-treated animals developed abundant pancreatic and lung edema,hemorrhage and leukocyte infiltrate,and pancreatic necrosis.Pancreatitis severity was greatly decreased by treating animals with an anti-TNF-αantibody,which diminished all inflammatory parameters,histopathological scores,and apoptotic/necrotic markers in stressed/cerulein-treated rats.CONCLUSION:In rats,chronic stress increases susceptibility for developing pancreatitis,which involves TNF-α sensitization of pancreatic acinar cells to undergo injury by physiological cerulein stimulation. | Marcelo G Binker Andres A Binker-Cosen Daniel Richards Herbert Y Gaisano Rodica H de Cosen Laura I Cosen-Binker | 2010 | World Journal of Gastroenterology2010，16，44： | 7 |
| 2 | New Insights Into the Mechanisms of Pancreatitis显示文摘 | Herbert Y. Gaisano Fred S. Gorelick | 2009 | Gastroenterology2009，，7： | 2 |
| 3 | Nitric oxide activation of a potassium channel (BK_(Ca)) in feline lower esophageal sphincter显示文摘AIM:To assess the effect of nitric oxide (NO) on the large conductance potassium channel (BKCa) in isolated circular (CM) and sling (SM) muscle cells and muscle strips from the cat lower esophageal sphincter (LES) to determine its regulation of resting tone and relaxation.METHODS:Freshly enzymatically-digested and isolated circular smooth muscle cells were prepared from each LES region.To study outward K + currents,the perforated patch clamp technique was employed.To assess LES resting tone and relaxation,muscle strips were mounted in perfused organ baths.RESULTS:(1) Electrophysiological recordings from isolated cells:(a) CM was more depolarized than SM (-39.7 ± 0.8mV vs-48.1 ± 1.6 mV,P < 0.001),and maximal outward current was similar (27.1 ± 1.5 pA/pF vs 25.7 ± 2.0 pA/pF,P > 0.05);(b) The NO donor sodium nitroprusside (SNP) increased outward currents only in CM (25.9 ± 1.9 to 46.7 ± 4.2 pA/pF,P < 0.001) but not SM (23.2 ± 3.1 to 27.0 ± 3.4 pA/pF,P > 0.05);(c) SNP added in the presence of the BK Ca antagonist iberiotoxin (IbTX) produced no increase in the outward current in CM (17.0 ± 2.8 vs 13.7 ± 2.2,P > 0.05);and (d) L-NNA caused a small insignificant inhibition of outward K + currents in both muscles;and (2) Muscle strip studies:(a) Blockade of the nerves with tetrodotoxin (TTX),or BK Ca with IbTX had no significant effect on resting tone of either muscle;and (b) SNP reduced tone in both muscles,and was unaffected by the presence of TTX or IbTX.CONCLUSION:Exogenous NO activates BK Ca only in CM of the cat.However,as opposed to other species,exogenous NO-induced relaxation is predominantly by a non-BK Ca mechanism,and endogenous NO has minimal effect on resting tone. | Marie-Claude L'Heureux Ahmad Muinuddin Herbert Y Gaisano Nicholas E Diamant | 2010 | World Journal of Gastroenterology2010，16，46： | 2 |
| 4 | Rac1 inhibition decreases the severity of pancreatitis and pancreatitis-associated lung injury in mice显示文摘 | BINKER M G BINKER COSEN A A GAISANO H Y | 2008 | Exp Physiol2008，93，10： | 1 |
| 5 | In- hibition of Racl decreases the severity of pancreati- tis and pancreatitis-associated lung injury in mice 显示文摘 | Binker MG Binker-Cosen AA Gaisano HY | 2008 | Exp Physiol2008，93，10： | 1 |
| 6 | Inhibi-tion of Rac 1 decreases the severity of pancreatitis and pan- creatitis associated lung injury in mice 显示文摘 | Binker MG Binker-Cosen AA Gaisano HY | 2008 | Exp Physiol2008，93，： | 1 |
| 7 | New insights into the mechanisms of pan- creatitis 显示文摘 | Gaisano H Y Gorelick F S | 2009 | Gastroenterology2009，136，7： | 1 |
| 8 | New insights into the mechanisms of pancreatitis显示文摘 | Gaisano H Y Gorelick F S | | 0，，07： | 1 |
| 9 | TGF-β in- creases invasiveness of SWl990 cells through Racl/ROS/ NF-k B/IL-6/MMP-2显示文摘 | Binker MG Binker-Cosen AA Gaisano HY | 2011 | Biochem Biophys Res Commun2011，405，1： | 1 |
| 10 | Mutations to the third cytoplasmaic domain of the glucagon-like peptide1(GLP-1)receptor can functionally uncouple GLP-1-stimulate dinsulinse cretionin HIT-T15cells显示文摘 | Salapatek AMF MacDonade PE Gaisano HY | 1999 | Mol Endocrinol1999，13，8： | 1 |
| 11 | VAMP8 is the v-SNARE that mediates basolateral exocytosis in a mouse model of alcoholic pancreatitis显示文摘 | Cosen-Binker Laura I Binker Marcelo G Wang Cheng-Chun Hong Wanjin Gaisano Herbert Y | 2008 | Journal of Clinical Investigation2008，，： | 1 |
| 12 | Hypoxia–reoxygenation increase invasiveness of PANC-1 cells through Rac1/MMP-2显示文摘 | Marcelo G. Binker Andres A. Binker-Cosen Daniel Richards Herbert Y. Gaisano Rodica H. de Cosen Laura I. Cosen-Binker | 2010 | Biochemical and Biophysical Research Communications2010，，3： | 1 |
| 13 | Glucagon-like peptide-1 inhibits voltage-gated potassium currents in mouse nodose ganglion neurons显示文摘 | GAISANO G G PARK S J DALY D M | 2010 | Neurogastroenterol Motil2010，22，： | 1 |
| 14 | Distinct regional expression of SNARE proteins in the feline oesophagus 显示文摘 | Ji J Lau H Sheu L Diamant NE Gaisano HY | 2002 | Neurogastroenterol Motil2002，14，4： | 1 |
| 15 | Syntaxin 1A regulates surface expression of beta-cell ATP-sensitive potassium channels显示文摘 | CHEN P C BRUEDERLE C E GAISANO H Y | 2011 | Am J Physiol Cell Phys-iol2011，300，3： | 1 |
| 16 | Inhibition of Rac1 decreases the severity of pancreatitis and pancreatitis-associated lung injury in mice显示文摘 | Binker M G Binker-Cosen A A Gaisano H Y | 2008 | Exp Physiol2008，93，10： | 1 |
| 17 | Inhibition of Racl decreases the severity of pancreatitis and pancreatitis- assoc iat-ed lung injury in mice 显示文摘 | Binker MG Binker-Cosen AA Gaisano HY | 2008 | Exp Physiol2008，93，10： | 1 |
| 18 | TGF-β1 increases invasiveness of SW1990 cells through Rac1/ROS/NF-κB/ IL-6/MMP-2显示文摘 | Binker MG Binker-Cosen AA Gaisano HY | 2011 | Biochem Biophys Res Commun2011，405，1： | 1 |
| 19 | Alcoholic chronic pancreatitis involves displacement of Muncl8c from the pancreatic acinar basal membrane surface 显示文摘 | Gaisano HY Sheu L Whitcomb D | 2004 | Pancreas2004，28，4： | 1 |
| 20 | Calcium source diversity in feline lower esophageal sphincter circular and sling muscle 显示文摘 | Muinuddin A Neshatian L Gaisano HY | 2004 | Am J Physiol Gastrointest Liver Physiol2004，286，2： | 1 |
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