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| 1 | Use of siRNA molecular beacons to detect and attenuate mycobacterial infection in macrophages显示文摘Tuberculosis is one of the leading infectious diseases plaguing mankind and is mediated by the facultative pathogen, Mycobacterium tuberculosis(MTB). Once the pathogen enters the body, it subverts the host immune defenses and thrives for extended periods of time within the host macrophages in the lung granulomas, a condition called latent tuberculosis(LTB). Persons with LTB are prone to reactivation of the disease when the body's immunity is compromised. Currently there are no reliable and effective diagnosis and treatment options for LTB, which necessitates new research in this area. The mycobacterial proteins and genes mediating the adaptive responses inside the macrophage is largely yet to be determined. Recently, it has been shown that the mce operon genes are critical for host cell invasion by the mycobacterium and for establishing a persistent infection in both in vitro and in mouse models of tuberculosis. The Yrb E and Mce proteins which are encoded by the MTB mce operons display high degrees of homology to the permeases and the surface binding protein of the ABC transports, respectively. Similarities in structure and cell surface location impute a role in cell invasion at cholesterol rich regions and immunomodulation. The mce4 operon is also thought to encode a cholesterol transport system that enables the mycobacterium to derive both energy and carbon from the host membrane lipids and possibly generating virulence mediating metabolites, thus enabling the bacteria in its long term survival within the granuloma. Various deletion mutation studies involving individual or whole mce operon genes have shown to be conferring varying degrees of attenuation of infectivity or at times hypervirulence to the host MTB, with the deletion of mce4 A operon gene conferring the greatest degree of attenuation of virulence. Antisense technology using synthetic si RNAs has been used in knocking down genes in bacteria and over the years this has evolvedinto a powerful tool for elucidating the roles of various genes mediating infectivity and survival in mycobacteria. Molecular beacons are a newer class of antisense RNA tagged with a fluorophore/quencher pair and their use for in vivo detection and knockdown of mR NA is rapidly gaining popularity. | Remo George Renata Cavalcante Celso Carvalho Jr Elyana Marques Jonathan B Waugh M Tino Unlap | 2015 | World Journal of Experimental Medicine2015，5，3： | 2 |
| 2 | Human neural tube defects: developmental biology, epidemiology, and genetics 显示文摘 | Detrait ER George TM Etchevers HC Gilbert JR Vekemans M Speer MC | 2005 | Neurotoxicol Teratol2005，27，3： | 1 |
| 3 | The ecology of medical care revisited显示文摘 | Larry A Green George E Fryer Jr Barbara P Yawn David lanier Susan M Dovey | 2001 | The New England Journal of Medicine2001，334，26： | 1 |
| 4 | piggyBac transposon-mediated gene transfer in human cells显示文摘 | Wilson M H Coates C J George Jr A L | 2007 | Mol Ther2007，15，： | 1 |
| 5 | Rapid evolution of animal mitochondrial DNA显示文摘 | Brown W M George M Jr Wilson A C | 1979 | Proc Natl Acad Sci USA1979，76，： | 1 |
| 6 | Antigenic and genetic char- acteristics of swine - origin 2009 A( H1N1 ) influenza viruses cir- culating in humans 显示文摘 | Garten R J Davis C T Russell C A Shu B Lindstrom S Balish A Sessions W M Xu X Skepner E Deyde V Okomo - Adhi- ambo M Gubareva L Barnes J Smith C B Emery S L Hillman M J Rivailler P Smagala J de Graaf M Burke D F Fouchier R A Pappas E Alpuche - Aranda C M Lopez - Gatell H Ohvera H Lopez I Myers C A Faix D Blair P J Yu C Keene K M Dotson P D Jr Boxrud D Sambol A R Abid S H St George K Bannerman T Moore A L Stringer D J Blevins P Demmler - Harrison G J Ginsberg M Kriner P Waterman S Smole S Guevara H F Belongia E A Clark P A Beatrice S T Donis R Katz J Finelli L Bridges C B Shaw M Jernigan D B Uyeki T M Smith D J Klimov A I Cox N J | 2009 | Science2009，325，5937： | 1 |
| 7 | piggyBac transposonmediated gene transfer in human cells显示文摘 | Wilson M H Coates C J George A L Jr | 2007 | Mol Ther2007，15，： | 1 |
| 8 | The effects of space and time resolution on the quality sea echo Doppler spectra measured with HF sky wave radar 显示文摘 | Jr | 1979 | Radio Science1979，14，3： | 1 |
| 9 | Human neural tube defects : developmental biology, epide- miology, and genetics显示文摘 | Detrait ER George TM Etchevers HC Gilbert JR Vekemans M Speer MC | 2005 | Neurotoxicol Teratol2005，27，3： | 1 |
| 10 | Complete genomic sequence of the virulent Salmonella bacteriophage SP6显示文摘 | Dobbins A T George M Jr Basham D A | 2004 | J Bacteriol2004，186，： | 1 |
| 11 | Rapid ew)lution of animal mitochondrial DNA显示文摘 | Brown W M George M Jr Wilson A C | 1979 | Pro Natl Acad Sci USA1979，76，4： | 1 |
| 12 | Rapid evolution of animal mitochondrial DNA 显示文摘 | BROWN W M JR GEORGE M WILSON A C | 1979 | Proceeding of the National Academy of Sciences of the United States of America1979，76，4： | 1 |
| 13 | Active control of mechanical vibrations显示文摘 | Roger D Benning Garry Hodgins M George G Zipfel Jr | 1997 | Bell Labs Technical Journal1997，2，2： | 1 |
| 14 | Rapid evolution of animal mitochondrial DNA显示文摘 | Brown W M George M Jr Wilson A C | 1979 | Proc Natl Acad Sci USA1979，76，4： | 1 |
| 15 | Primary structure and functional expression of the human cardiac tetrodotoxin-insensitlve voltage-dependent sodium channel显示文摘 | Gellens ME George AL Jr Chen LQ Chahine M Horn R Barchi BL | 1992 | Proc Natl Acad Sci USA1992，89，2： | 1 |
| 16 | The effects of space and time resolution on the quality sea echo Doppler spectra measured with HF sky wave radar 显示文摘 | T M Georges and J W Maresca Jr | 1979 | Radio Science1979，14，3： | 1 |
| 17 | Intraneural epithelial islands associated with a periapical cyst显示文摘 | George D I Jr Gould A R Behr M M | 1984 | Oral Surg Oral Med Oral Pathol1984，57，1： | 1 |
| 18 | Impact of Genetics on the Clinical Management of Channelopathies显示文摘 | Schwartz P J Ackerman M J George A L Jr | | 0，，03： | 1 |
| 19 | Functional domains of the Drosophila melanogaster muscle myosin heavy-chain gene are encoded by alternatively spliced exons显示文摘 | George E L Ober M B Emerson C P Jr | 1989 | Mol Cell Biol1989，9，7： | 1 |
| 20 | Rapid evolution of animal mitochondrial DNA显示文摘 | Brown W M George M Jr Wilson A C | 1989 | Trend Ecol Evol1989，，4： | 1 |
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