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| 1 | Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG显示文摘Two recently engineered SpCas9 variants, namely xCas9 and Cas9-NG, show promising potential in improving targeting specificity and broadening the targeting range. In this study, we evaluated these Cas9 variants in the model and crop plant, rice. We first tested xCas9-3.7, the most effective xCas9 variant in mammalian cells, for targeted mutagenesis at 16 possible NGN PAM (protospacer adjacent motif) combinations in duplicates. xCas9 exhibited nearly equivalent editing efficiency to wild-type Cas9 (Cas9-WT) at most canonical NGG PAM sites tested, whereas it showed limited activity at non-canonical NGH (H = A, C, T) PAM sites. High editing efficiency of xCas9 at NGG PAMs was further demonstrated with C to T base editing by both rAPOBECI and PmCDAI cytidine deaminases. With mismatched sgRNAs, we found that xCas9 had improved targeting specificity over the Cas9-WT. Furthermore, we tested two Cas9-NG variants, Cas9-NGv1 and Cas9-NG, for targeting NGN PAMs. Both Cas9-NG variants showed higher editing efficiency at most non-canonical NG PAM sites tested, and enabled much more efficient editing than xCas9 at AT-rich PAM sites such as GAT, GAA, and CAA. Nevertheless, we found that Cas9-NG variants showed significant reduced activity at the canonical NGG PAM sites. In stable transgenic rice lines, we demonstrated that Cas9-NG had much higher editing efficiency than Cas9-NGv1 and xCas9 at NG PAM sites. To expand the base-editing scope, we developed an efficient C to T base-editing system by making fusion of Cas9-NG nickase (D10A version), PmCDAI, and UGI. Taken together, our work benchmarked xCas9 as a high-fidelity nuclease for targeting canonical NGG PAMs and Cas9-NG as a preferred variant for targeting relaxed PAMs for plant genome editing. | Zhaohui Zhong Simon Sretenovic Qiurong Ren Lijia Yang Yu Bao Caiyan Qi Mingzhu Yuan Yao He Shishi Liu Xiaopei Liu Jiaheng Wang Lan Huang Yan Wang Dibin Baby David Wang Tao Zhang Yiping Qi Yong Zhang | 2019 | Molecular Plant2019，12，7： | 13 |
| 2 | Variation at the transcriptional level among Chinese natural populations of Arabidopsis thaliana in response to cold stress显示文摘The Arabidopsis 25K GeneChip (ATH1, Affymetrix) was used to make a survey of the variation of the transcriptional profiles among 5 Chinese natural populations of Arabidopsis thaliana under cold treatment. In normal growth condition, the expression level of 2.26% (513 genes in the population from Jiujiang, Jiangxi, JXjjx) to 6.52% (1482 genes in the population from Tongliang, Chongqing, CQtlx) genes was 2-fold higher than that of Col ecotype. Under cold treatment, the expression of 12.84% (2920 genes in the population from Chenggu, Shaanxi, SXcgx) to 19.46% (4426 genes in the population from Qinghe, Xinjiang, XJqhx) genes was up- or down-regulated by at least two-fold that of their controls. In general, most of up-regulated genes might be the genes essential for plant surviving at low temperature, such as genes in CBF pathway and the genes responsible for synthesizing molecules accumulated for cold tolerance. However, each natural population had some specific genes induced under cold treatment. The data indicated that some of the cold-responding genes were differentiated among the populations distributed in the natural habitats with different climate conditions. CBF3, one of the key tran-scription factor genes in cold responding pathway, showed significant differences in expression among populations. The sequence analysis indicated that the changes in its regulation region caused the dramatic difference in the expression pattern. Further studies on the correlation of the function of the differentially expressed genes and the cold tolerance in different populations may provide some new insight into the molecular mechanism of adaptation to local environment in Arabidopsis thaliana in China. | HE Fei KANG JiuQing ZHOU Xin SU Zhen QU LiJia GU HongYa | 2008 | Chinese Science Bulletin2008，53，19： | 9 |
| 3 | Influences of electric pulse on solidification structure of LM-29 Al-Si alloy显示文摘The metallographic structure of LM-29 aluminum-silicon alloy modified by electric pulse treatment has been investigated and compared with those untreated.The solidification structure of LM-29 alloy has been analyzed by means of M1AP3 Quantimet image processing and analysis system,and then the solidification process has been analyzed by means of differential scanning calorimetry(DSC).The results indicate that the primary silicon phase was refined remarkably by electric pulse while the tensile strength and elongation properties increased accordingly.Electric pulse treatment can also increase the binding power between silicon clusters and alloy melt matrix,as a result,the precipitation of primary silicon phase is suppressed to meet the demand of supercooling degree for nucleating,correspondingly.The electric pulse modification has great influence on the size of silicon atomic cluster as well as its distribution in the melt,subsequently,leads to the refinement of solidification structure. | He Lijia Wang Jianzhong Qi Jingang Du Huiling Zhao Zuofu | 2010 | China Foundry2010，7，2： | 5 |
| 4 | EET research on the Al-22%Si alloy under the action of electric pulses显示文摘Based on the empirical electron theory of solids and molecules (EET), the phase valence electron structure parameters of Al-22%Si alloy are calculated, and the sensitivity of the bond network of Al-Si alloy melt to temperature (energy) and the effect of the sensitivity on the morphology are studied. The results show that the Si-Si clusters with larger nA in the slightly superheated Al-Si alloy melt supply the nucleation core to the primary silicon phase in the hypereutectic Al-22%Si alloy, and strongly generate the drag-like effect for the Al-Si clusters around them; that the variation of temperature significantly affects the stability of bonds of the core so that the solidified structure is changed; that the electric pulse applied to the alloy melt can irrecoverably alter the stability of Si-Si clusters, then the modifica- tion of the solidified structure morphology of alloys is generated; that the higher the energy of the electric pulse, the less stable the Si-Si clusters, and the more significant the electric pulse modification. | WANG JianZhong HE LiJia LIN Cheng CANG DaQiang | 2008 | Science China(Technological Sciences)2008，51，11： | 4 |
| 5 | Laser-based micro/nanofabrication in one, two and three dimensions显示文摘有各种各样的尺寸的功能的材料和结构的先进 micro/nanofabrication 在现代 nanoscience 和技术代表一个关键研究话题并且为象 nanoelectronics, nanophotonics 和 micro/nanoelectromechanical 系统那样的众多的出现技术变得极其重要。这评论系统地探索在制作挑战性经由常规途径被制作的各种各样的尺寸的 nanomaterials 和 micro/nanostructures 处理途径的非常规的材料。研究努力为生长和制造集中于基于激光的技术一个维(1D ) ,二维(2D ) 并且三维(3D ) nanomaterials 和 micro/nanostructures。下列研究话题被论及,包括:1 ) 为碳 nanotubes (CNT ) 的 1D nanomaterial 的高度有效的生长和集成的帮助激光的化学蒸汽免职(CVD ) , 2 ) 在周围的条件下面的 graphene 带子的直接写的激光(LDW ) ,并且 3 ) 经由添加剂和减少性的过程的 3D micro/nanostructures 的 LDW。与常规制造方法作比较,基于激光的方法在先进功能的材料和结构的 micro/nanofabrication 展出几个唯一的优点。为 1D CNT 生长,帮助激光的 CVD 进程能由于高度强烈的精力交货和导致激光的光近地的效果认识到两快速的材料合成和生长地点的紧密的控制和 CNT 的取向。为 2D graphene 合成和 patterning,房间温度并且露天绝缘的表面上的大规模 graphene 模式的制造被一个 LDW 过程成功地认识到。为 3D micro/nanofabrication,二光子的聚合(TPP ) 和减少性的多光子脱离(MPA ) 处理的添加剂的联合启用为常规制造方法挑战性的任意的复杂 3D micro/nanostructures 的制造。就基于激光的技术的众多的唯一的优点而言,基于激光的 micro/nanofabrication 被期望在先进功能的 micro/nano-devices 的制造起一个越来越重要的作用。 | Wei XIONG Yunshen ZHOU Wenjia HOU Lijia JIANG Masoud MAHJOURI-SAMANI Jongbok PARK Xiangnan HE Yang GAO Lisha FAN Tommaso BALDACCHINI Jean-Francois SILVAIN Yongfeng LU | 2015 | Frontiers of Optoelectronics2015，8，4： | 4 |
| 6 | The cluster size transformation model of molten alloy under pulse electric field显示文摘Based on the electric dipole theory, the coupled field distribution of pulse electric field (PEF) with electric dipole field around the cluster in superheated molten alloy is simulated under the effect of PEF. For the difference of electro-migration, the atom of solute and solution will accumulate around the cluster, and then the smaller cluster may reorganize and grow up under the action of the coupled field. We also apply the electrostatic induction theory to analyze the bearing behavior of the half side of the cluster. The bigger the cluster is, the stronger the electrostatic force is, therefore, the bigger cluster's stability is weak apparently. The study indi- cates that the cluster in the superheated molten has the homogeneous tendency under the effect of PEF. | ZHANG ZhenBin WANG JianZhong QI JinGang WANG Bing HE LiJia CANG DaQiang | 2008 | Science China(Technological Sciences)2008，51，3： | 2 |
| 7 | E3 ligase FBXW7 aggravates TMPD-induced systemic lupus erythematosus by promoting cell apoptosis显示文摘Systemic lupus erythematosus(SLE)is a systemic autoimmune disease,and the pathogenesis of SLE has not been fully elucidated.The E3 ubiquitin ligase FBXW7 has been well characterized in cancer as a tumor suppressor that can promote the ubiquitination and subsequent degradation of various oncoproteins;however,the potential role of FBXW7 in autoimmune diseases is unclear.In the present study,we identified that FBXW7 is a crucial exacerbating factor for SLE development and progression in a mouse model induced by 2,6,10,14-tetramethylpentadecane(TMPD).Myeloid cell-specific FBXW7-deficient(Lysm+FBXW7f/f)C57BL/6 mice showed decreased immune complex accumulation,glomerulonephritis,glomerular mesangial cell proliferation,and basemembrane thickness in the kidney.Lysm+FBXW7f/f mice produced fewer anti-Sm/RNP and anti-ANA autoantibodies and showed a decreased MHC II expression in B cells.In Lysm+FBXW7f/f mice,we observed that cell apoptosis was reduced and that fewer CD11b+Ly6Chi inflammatory monocytes were recruited to the peritoneal cavity.Consistently,diffuse pulmonary hemorrhage(DPH)was also decreased in Lysm+FBXW7f/f mice.Mechanistically,we clarified that FBXW7 promoted TMPD-induced cell apoptosis by catalyzing MCL1 degradation through K48-linked ubiquitination.Our work revealed that FBXW7 expression in myeloid cells played a crucial role in TMPD-induced SLE progression in mice,which may provide novel ideas and theoretical support for understanding the pathogenesis of SLE. | Zhenlu Chong Chunjing Bao Jia He Tianxiao Chen Lijia Zhong Gaopeng Li Huanle Li Lutong Fang Yinjing Song Guoxiang Fu Xuyan Yang Lihua Lai Yang Liu Qingqing Wang | 2018 | Cellular & Molecular Immunology2018，15，12： | 2 |
| 8 | Research of EET on the Al-22%Si alloy under electric pulse 显示文摘 | Wang Jianzhong He Lijia Cang Daqiang | 2008 | Science in China Series E2008，51，11： | 1 |
| 9 | One-to-one quantum dot-labeled single long DNA probes显示文摘 | Shibin He Bi-Hai Huang Junjun Tan Qing-Ying Luo Yi Lin Jun Li Yong Hu Lu Zhang Shihan Yan Qi Zhang Dai-Wen Pang Lijia Li | 2011 | Biomaterials2011，，23： | 1 |
| 10 | PbS/epoxy Resin Nanocomposite Prepared by a Novel Method显示文摘 | Lijia Pan Pingsheng He Gang Zou | 2004 | Materials Letters2004，58，2： | 1 |
| 11 | Single-walled carbon nanotubes selectively influence maize root tissue development accompanied by the change in the related gene expression显示文摘 | Shihan Yan Lin Zhao Hui Li Qi Zhang Junjun Tan Min Huang Shibin He Lijia Li | 2013 | Journal of Hazardous Materials2013，，： | 1 |
| 12 | Amorphous calcium magnesium phosphate nanocomposites with superior osteogenic activity for bone regeneration显示文摘The seek of bioactive materials for promoting bone regeneration is a challenging and longterm task.Functionalization with inorganic metal ions or drug molecules is considered effective strategies to improve the bioactivity of various existing biomaterials.Herein,amorphous calcium magnesium phosphate(ACMP)nanoparticles and simvastatin(SIM)-loaded ACMP(ACMP/SIM)nanocomposites were developed via a simple co-precipitation strategy.The physiochemical property of ACMP/SIM was explored using transmission electron microscope(TEM),Fourier transform infrared spectroscopy(FTIR),powder X-ray diffraction(XRD)and highperformance liquid chromatograph(HPLC),and the role of Mg^(2+) in the formation of ACMP/SIM was revealed using X-ray absorption near-edge structure(XANES).After that,the transformation process of ACMP/SIM in simulated body fluid(SBF)was also tracked to simulate and explore the in vivo mineralization performance of materials.We find that ACMP/SIM releases ions of Ca^(2+),Mg^(2+)and PO_(4)^(3),when it is immersed in SBF at 37℃,and a phase transformation occurred during which the initially amorphous ACMP turns into self-assembled hydroxyapatite(HAP).Furthermore,ACMP/SIM displays high cytocompatibility and promotes the proliferation and osteogenic differentiation of MC3T3-E1 cells.For the in vivo studies,lamellar ACMP/SIM/Collagen scaffolds with aligned pore structures were prepared and used to repair a rat defect model in calvaria.ACMP/SIM/Collagen scaffolds show a positive effect in promoting the regeneration of calvaria defect after 12weeks.The bioactive ACMP/SIM nanocomposites are promising as bone repair materials.Considering the facile preparation process and superior in vitro/vivo bioactivity,the as-prepared ACMP/SIM would be a potential candidate for bone related biomedical applications. | Yingying Jiang Shuo Tan Jianping Hu Xin Chen Feng Chen Qianting Yao Zhi Zhou Xiansong Wang Zifei Zhou Yunshan Fan Junjian Liu Yize Lin Lijia Liu Shisheng He | 2021 | Regenerative Biomaterials2021，8，6： | 0 |
| 13 | Interface profile evolution between binary immiscible fluids induced by high magnetic field gradients显示文摘A mechanical analysis is done to find the evolution of the interface profile between binary immiscible fluids induced by a three-dimensional orthogonal magnetic field gradient.In the experiments,the changes of the interface profile between four groups of binary immiscible fluids are investigated under the same horizontal magnetic field gradients.The binary immiscible fluids are made of benzene and other liquids,like CuSO4,Fecl3,FeSO4 or Cucl2 aqueous solutions.In addition,the interface profile between the benzene and CuSO4 aqueous solution is examined under different horizontal magnetic field gradients.The experimental results are consistent with the theoretical analysis.This study explains the enhanced Moses effect from a mechanics standpoint.Furthermore,a new method for susceptibility measurement is proposed based on this enhanced Moses effect. | ZHAO LiJia,YOU Yue,TIAN YongHua,YANG HongKai,WANG Qiang * & HE JiCheng Key Laboratory of Electromagnetic Processing of Materials(Ministry of Education),Northeastern University,Shenyang 110004,China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，7： | 0 |
| 14 | Time-series variation and attribution analysis of downward shortwave radiation over the Yunnan-Kweichow plateau from 1984 to 2018显示文摘The downward shortwave radiation(DSR)is a key input parameter for land surface models and climate models.Based on the daily averaged Global Land Surface Satellite downward shortwave radiation(GLASS-DSR)dataset over the Yunnan-Kweichow Plateau(YKP)from 1984 to 2018,this paper analyzes variation trend and breakpoints of DSR.The results show that:annual averaged DSR decreases at a decreasing rate of-1.84 W·m^(-2)·decade^(-1) over the YKP from 1984 to 2018;the overall distribution of interannual averaged DSR shows higher in the mid-west,and gradually decreasing from west to northeast over the YKP;the estimated averaged DSR is larger in spring than in summer due to the influence of the monsoon;monthly averaged DSR reaches its maximum in May and its minimum in December;breakpoints are found in the seasonal and trend components of daily averaged DSR.Eleven driving factors are examined for their effects on DSR variation,including annual average temperature,precipitation,10 m wind speed,aerosol optical thickness(AOT),total cloud cover,elevation,slope,aspect,longitude,latitude,and climate zones.According to thefindings,AOT predominates in the spatio-temporal distribution of DSR over the YKP.This study will contribute to studies related to climate change and highland radiation. | Lijia Cheng Bo-Hui Tang Zhiwei He Zhitao Fu Menghua Li | 2023 | International Journal of Digital Earth2023，16，1： | 0 |
| 15 | Visible-Light-Promoted[3+2]Cycloaddition of 2H-Azirines with Quinones:Access to Substituted Benzo[f]isoindole-4,9-diones显示文摘A visible-light-promoteded[3+2]cycloaddition reaction of 2H-azirines with quinones has been developed under mild reaction con-ditions.The reaction provides a general and efficient strategy for the synthesis of the benzo[f]isoindole-4,9-diones scaffold via a tandem[3+2]cyclization/oxidative aromatization with molecular oxygen.Furthermore,preliminary studies for photocatalytic prop-erties show that the benzo[f]isoindole-4,9-diones 3 can be used as photocatalysts in several organic transformations. | Lijia Wang Chuang Liu Lei Li Xin Wang Ran Sun Ming-Dong Zhou He Wang | 2022 | Chinese Journal of Chemistry2022，40，6： | 0 |
| 16 | Study the relationship between the expression of MRP and the radiosensitivity of primary lesions of nasopharyngeal carcinoma显示文摘Objective: To study the relationship between the expression of multidrug resistance-associated protein (MRP) gene in nasopharyngeal carcinoma (NPC) and the radiosensitivity of primary lesions of NPC. Methods: Total 51 cases of NPC were analyzed retrospectively, who were treated by radiotherapy between January 1, 1999 and December 31, 2000 in our department, including the cases of pure radiotherapy and chemotherapy after radiotherapy. Using immunohistochemiscal S-P method, the expression of MRP in NPC was detected, and the relationship with the radiosensitivity of primary lesions of NPC was analyzed. Results: The rate of positive expression was 68.63% (35/51) in NPC, and there were significant difference between T stages (P<0.05), but no significant difference with radiosensitivity of primary lesions of NPC (P>0.05). Conclusion: There is a higher expression of MRP in NPC, but it has no relationship with radiosensitivity of primary lesions of NPC. So MRP can not be regarded as the predictive marker of radiosensitivity of primary lesions of NPC. | Jianwen Zhang Jingbo Wu Juan Fan Xueqing Tang Lijia He Yanlin Zhang | 2006 | The Chinese-German Journal of Clinical Oncology2006，5，5： | 0 |
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