| 2 | A cyclin protein governs the infectious and sexual life cycles of Cryptococcus neoformans显示文摘Cell cycle is a fundamental process underlying growth and development in evolutionarily diverse organisms, including fungi. In human fungal pathogens, cell cycle control generally determines their life cycles, either in the environment or during infections.Thus, cell cycle components can potentially serve as important targets for the development of antifungal strategy against fungal infections. Here, in Cryptococcus neoformans, the most common cause of fatal fungal meningitis, we show that a previously uncharacterized B-type cyclin named Cbc1 is essential for both its infectious and sexual cycles. We reveal that Cbc1 coordinates various sexual differentiation and molecular processes, including meiosis. Especially, the absence of Cbc1 abolishes formation of sexual spores in C. neoformans, which are presumed infectious particles. Cbc1 is also required for the major Cryptococcus pathogenic attributes. Virulence assessment using the murine model of cryptococcosis revealed that the cbc1 mutant is avirulent.Together, our results provide an important insight into how C. neoformans employs shared cell cycle regulation to coordinate its infectious and sexual cycles, which are considered crucial for virulence evolution and the production of infectious spores. | Pengjie Hu Linxia Liu Weixin Ke Xiuyun Tian Linqi Wang | 2021 | Science China(Life Sciences)2021，64，8： | 1 |
| 3 | Single-cell RNA-seq of Lotus japonicus provide insights into identification and function of root cell types of legume显示文摘The roots of legume plant play a crucial role in nitrogen fixation. However, the transcriptomes of different cell types of legume root and their functions remain largely unknown. Here, we performed single-cell RNA sequencing and profiled more than 22,000 single cells from root tips of Lotus japonicus, a model species of legume.We identified seven clusters corresponding to seven major cell types, which were validated by in situ hybridization. Further analysis revealed regulatory programs including phytohormone and nodulation associated with specific cell types, and revealed conserved and diverged features for the cell types. Our results represent the first single-cell resolution transcriptome for legume root tips and a valuable resource for studying the developmental and physiological functions of various cell types in legumes. | Zhanmin Sun Sanjie Jiang Dan Wang Linxia Li Boxin Liu Qifan Ran Lizhen Hu Junbo Xiong Yixiong Tang Xiaofeng Gu Yanmin Wu Zhe Liang | 2023 | Journal of Integrative Plant Biology2023，65，5： | 0 |
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