|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | An update on primary ovarian insufficiency显示文摘Primary ovarian insufficiency(POI) occurs in about 1% of female population under the age of 40,leading to reproductive problems,an earlier encounter with menopausal symptoms,and complicated diseases.There are three presumable mechanisms involved in the development of POI,namely apoptosis acceleration,follicular maturation blocking and premature follicle activation,through the following studied causes:(i) chromosomal abnormalities or gene mutations:mostly involve X chromosome,such as FMR1 premutation;more and more potentially causal genes have been screened recently;(ii) metabolic disorders such as classic galactosaemia and 17-OH deficiency;(iii) autoimmune mediated ovarian damage:observed alone or with some certain autoimmune disorders and syndromes;but the specificity and sensitivity of antibodies towards ovary are still questionable;(iv) iatrogenic:radiotherapy or chemotherapy used in cancer treatment,as well as pelvic surgery with potential threat to ovaries' blood supply can directly damage ovarian function;(v) virus infection such as HIV and mumps;(vi) toxins and other environmental/lifestyle factors:cigarette smoking,toxins(e.g.,4-vinylcyclohexene diepoxide),and other environmental factors are associated with the development of POI.The etiology of a majority of POI cases is not identified,and is believed to be multifactorial.Strategies to POI include hormone replacement and infertility treatment.Assisted conception with donated oocytes has been proven to achieve pregnancy in POI women.Embryo cryopreservation,ovarian tissue cryopreservation and oocyte cryopreservation have been used to preserve ovarian reserve in women undergoing cancer treatments. | JIN Min YU YiQi HUANG HeFeng | 2012 | Science China(Life Sciences)2012，55，8： | 22 |
| 2 | Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer显示文摘Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extremely inefficient.Zinc-finger nuclease(ZFN)technology has been shown to be a powerful tool for efficiently inducing mutations in the genome.However,ZFN-mediated targeting in pigs has rarely been achieved.Here,we used ZFNs to knock out the porcineα-1,3-galactosyl-transferase(GGTA1)gene,which generates Gal epitopes that trigger hyperacute immune rejection in pig-to-human transplantation.Primary pig fibroblasts were transfected with ZFNs targeting the coding region of GGTA1.Eighteen mono-allelic and four biallelic knockout cell clones were obtained after drug selection with efficiencies of 23.4%and 5.2%,respectively.The biallelic cells were used to produce cloned pigs via somatic cell nuclear transfer(SCNT).Three GGTA1 null piglets were born,and one knockout primary fibroblast cell line was established from a cloned fetus.Gal epitopes on GGTA1 null pig cells were completely eliminated from the cell membrane.Functionally,GGTA1 knockout cells were protected from complement-mediated immune attacks when incubated with human serum.This study demonstrated that ZFN is an efficient tool in creating gene-modified pigs.GGTA1 null pigs and GGTA1 null fetal fibroblasts would benefit research and pig-to-human transplantation. | BAO Lei CHEN HaiDe JONG UiMyong RIM CholHo LI WenLing LIN XiJuan ZHANG Dan LUO Qiong CUI Chun HUANG HeFeng ZHANG Yan XIAO Lei FU ZhiXin | 2014 | Science China(Life Sciences)2014，57，2： | 9 |
| 3 | Advances in research into gamete and embryo-fetal origins of adult diseases显示文摘The fetal and infant origins of adult disease hypothesis proposed that the roots of adult chronic disease lie in the effects of adverse environments in fetal life and early infancy. In addition to the fetal period, fertilization and early embryonic stages, the critical time windows of epigenetic reprogramming, rapid cell differentiation and organogenesis, are the most sensitive stages to environmental disturbances. Compared with embryo and fetal development, gametogenesis and maturation take decades and are more vulnerable to potential damage for a longer exposure period. Therefore, we should shift the focus of adult disease occurrence and pathogenesis further back to gametogenesis and embryonic development events, which may result in intergenerational, even transgenerational, epigenetic re-programming with transmission of adverse traits and characteristics to offspring. Here, we focus on the research progress relating to diseases that originated from events in the gametes and early embryos and the potential epigenetic mechanisms involved. | Kexin Zou Guolian Ding Hefeng Huang | 2019 | Science China(Life Sciences)2019，62，3： | 7 |
| 4 | A combination of follicle stimulating hormone, estradiol and age is associated with the pregnancy outcome for women undergoing assisted reproduction: a retrospective cohort analysis显示文摘To investigate the effect of basal hormone levels including follicle stimulating hormone(FSH) and estradiol(E2) and age on the pregnancy outcomes in women undergoing in vitro fertilization and embryo transfer(IVF-ET), we designed a retrospective cohort analysis and collected information of patients undergoing IVF between January 2014 and April 2017. We found that young women had much better pregnancy outcomes than older women regardless of the hormone levels. The pregnancy outcomes declined in young women with high basal E2, even though they had normal FSH. Older women with higher FSH had a worse outcome of pregnancy, especially with increased E2 level. The results suggest that a combination of FSH, E2 and age could effectively predict the pregnancy outcome for women undergoing IVF-ET, and we should encourage infertile women with diminished ovarian reserve, especially young infertile women, to try the assisted reproduction as early as possible. After 40-yearold, the cost-benefit ratio should be considered according to the ovarian function. | Ziru Jiang Li Jin Weihui Shi Ji Xi Yulian Hu Xinmei Liu Jianzhong Sheng Guolian Ding Hefeng Huang | 2019 | Science China(Life Sciences)2019，62，1： | 7 |
| 5 | Recent advances in mammalian reproductive biology显示文摘Reproductive biology is a uniquely important topic since it is about germ cells, which are central for transmitting genetic information from generation to generation. In this review, we discuss recent advances in mammalian germ cell development,including preimplantation development, fetal germ cell development and postnatal development of oocytes and sperm. We also discuss the etiologies of female and male infertility and describe the emerging technologies for studying reproductive biology such as gene editing and single-cell technologies. | Lu Wen Qiang Liu Jingjing Xu Xixi Liu Chaoyi Shi Zuwei Yang Yili Zhang Hong Xu Jiang Liu Hui Yang Hefeng Huang Jie Qiao Fuchou Tang Zi-Jiang Chen | 2020 | Science China(Life Sciences)2020，63，1： | 6 |
| 6 | Mechanism underlying the retarded nuclear translocation of androgen receptor splice variants显示文摘As shown in our previous study, two alternatively spliced androgen receptor(AR) variants, which are exclusively expressed in the granulosa cells of patients with polycystic ovary syndrome, exhibit retarded nuclear translocation compared with wild-type AR. However, researchers have not yet determined whether these abnormalities correlate with heat shock protein 90(HSP90)and importin α(the former is a generally accepted co-chaperone of AR, and the latter is a component of classical nuclear import complexes). Here, these two variants were mainly retained in cytoplasm with HSP90 and importin α in the presence of dihydrotestosterone(DHT), and their levels in nucleus were significantly reduced, according to the immunofluorescence staining. The binding affinity of two AR variants for importin α was consistently decreased, while it was increased in WT-AR following DHT stimulation, leading to reduced nuclear import, particularly for the insertion-AR(Ins-AR). However, the binding affinities of two AR variants for HSP90 were increased in the absence of DHT compared with WT-AR, which functioned to maintain spatial structural stability, particularly for the deletion-AR(Del-AR). Therefore, the retarded nuclear translocation of two AR variants is associated with HSP90 and importin α, and the abnormal binding affinities for them play critical roles in this process. | Ye Liu Yinyu Wang Fangfang Wang Jiexue Pan Jingjing Xu Jingyi Li Chengliang Zhou Guolian Ding Yanting Wu Xinmei Liu Jianzhong Sheng Hefeng Huang | 2019 | Science China(Life Sciences)2019，62，2： | 4 |
| 7 | Distribution characteristics and its controlling factor of lacustrine high-quality source rocks in the Bozhong sag,Bohai Bay Basin显示文摘Distribution of Paleogene lacustrine high-quality source rocks in the Bozhong sag in Bohai Bay Basin is analyzed through data of geochemistry,geology and well logging,and its differences under the control of climate and tectonics is also well discussed.Distribution characteristics of the high-quality source rocks developed in the saline environment controlled by the climate are quite different from that developed in the rapid subsidence environment controlled by tectonics.The high-quality source rocks in Member 1 of Shahejie Formation developed in the saline environment account for 81.9%of total subsag area,and are distributed widely and extensively.The high-quality source rocks in Member 3 of Shahejie Formation and Member 3 of Dongying Formation is developed in the tectonic subsidence environment,and horizontally,the subsag subsidence rates has a positive correlation with the area proportion of the high-quality source rocks in the sag;vertically,the reduction-oxidation interface of the lake controls the enrichment of highquality source rocks.Controlled by the saline environment and rapid subsidence environment,the highquality source rocks in the Bozhong sag and adjacent areas have three types of development condition:the saline water,the tectonic subsidence,and joint control of the saline water and the tectonic subsidence.The humid climate and low subsidence rate are not favorable for development of high-quality source rocks. | Hefeng Sun Lifang Liu Xue Jiang Shengbing Huang Mingyang Geng Shaoping Chen Nan Li Pu Shen | 2019 | Petroleum Research2019，4，3： | 3 |
| 8 | High expression of follicle stimulating hormone receptor in testicular tissue of idiopathic azoospermic patients with severe spermatogenic defects显示文摘 | Wang Liquan Huang Hefeng Jin Fan Zhou Caiyun Qian Yuli Chen Jianhua | 2014 | Chinese Medical Journal2014，，3： | 3 |
| 9 | Dietary fibers with low hydration properties exacerbate diarrhea and impair intestinal health and nutrient digestibility in weaned piglets显示文摘Background:This study aimed to investigate the hydration properties of different-source fibrous materials by com-paring their water-binding capacity(WBC),water swelling capacity(WSC),viscosity,and in vivo effects of selected samples on growth performance,nutrient digestibility,diarrhea,and intestinal health in weaned piglets.Methods:A total of 13 commercially available fibrous materials were first compared in chemical composition and in vitro hydration property.Subsequently,40 weaned piglets were randomized to five experimental dietary groups(8 piglets per group):control diet(a basal diet without dietary fiber,CON),basal diet supplemented with 5%microcrys-talline cellulose(MCC),5%wheat bran(WB),5%Moringa oleifera leaf powder(MOLP),or 5%sugar beet pulp(SBP),fol-lowed by analyzing their growth performance and diarrhea rate in a 28-d experiment.After the feeding experiment,anaesthetized piglets were killed,and their intestinal and colon content or plasma samples were analyzed in nutrient digestibility,intestinal morphology,intestinal barrier,short-chain fatty acids(SCFAs),and bacterial population.Results:In vitro studies showed low hydration properties for WB and MCC,while medium hydration properties for MOLP and SBP.In vivo studies indicated that compared with medium hydration property groups,low hydration prop-erty groups showed(1)exacerbated diarrhea,impaired intestinal health,and reduced apparent fecal digestibility of dry matter,gross energy,acid detergent fiber,and neutral detergent fiber;(2)decreased SCFAs concentration and rela-tive levels of Lactobacillus and Bifidobacterium,but increased levels of Escherichia coli and Brachyspira hyodysenteriae in colon contents.Additionally,SBP showed optimal performance in reducing diarrhea and increasing SCFAs produc-tion.Correlation analysis revealed a positive correlation of fiber hydration properties with in vitro SCFAs production,and diarrhea index and nutrient digestibility were negatively and positively correlated with SCFAs levels in the colon contents of weaned piglets,respectively.Conclusions:Different-source dietary fibers varied in their hydration properties and impacts on diarrhea,microbial composition and SCFAs production in weaned piglets.WB and MCC could exacerbate diarrhea and impair nutrient digestibility,probably because their low hydration properties were detrimental to gut microbial homeostasis and fermentation.Our findings provide new ideas for rational use of fiber resources in weaned piglets. | Shuangbo Huang Zhijuan Cui Xiangyu Hao Chuanhui Cheng Jianzhao Chen Deyuan Wu Hefeng Luo Jinping Deng Chengquan Tan | 2023 | Journal of Animal Science and Biotechnology2023，14，2： | 1 |
| 10 | Clinical application of massively parallel sequencing‐based prenatal noninvasive fetal trisomy test for trisomies 21 and 18 in 11 105 pregnancies with mixed risk factors显示文摘 | Shan Dan Wei Wang Jinghui Ren Yali Li Hua Hu Zhengfeng Xu Tze Kin Lau Jianhong Xie Weihua Zhao Hefeng Huang Jiansheng Xie Luming Sun Xiaohong Zhang Weipeng Wang Shixiu Liao Rong Qiang Jiangxia Cao Qiufang Zhang Yulin Zhou Haiyan Zhu Mei Zhong Yi Guo Linhu | 2012 | Prenat Diagn2012，，13： | 1 |
| 11 | A new model for embryoimplantation:coculture of blastocysts and Ishikawa cells显示文摘 | Dan Zhang Pingping Lv Hefeng Huang | 2011 | Gyneco- logical Endocrinology2011，28，4： | 1 |
| 12 | Clinical application of massively parallel sequencing‐based prenatal noninvasive fetal trisomy test for trisomies 21 and 18 in 11 105 pregnancies with mixed risk factors显示文摘 | Shan Dan Wei Wang Jinghui Ren Yali Li Hua Hu Zhengfeng Xu Tze Kin Lau Jianhong Xie Weihua Zhao Hefeng Huang Jiansheng Xie Luming Sun Xiaohong Zhang Weipeng Wang Shixiu Liao Rong Qiang Jiangxia Cao Qiufang Zhang Yulin Zhou Haiyan Zhu Mei Zhong Yi Guo Linhu | 2012 | Prenat Diagn2012，，13： | 1 |
| 13 | Intrauterine hyperglycemia impairs endometrial receptivity via up-regulating SGK1 in diabetes显示文摘Diabetes is a complex metabolic disorder which can adversely affect reproductive function. SGK1 is found to be up-regulated in multiple tissues of diabetic patients. However, the effects of diabetes on endometrial SGK1 expression and endometrial receptivity remain unknown. In this study, we established a streptozotocin-induced diabetic mouse model and observed reduced implantation sites, retarded development of pinopodes, increased SGK1, and aberrant expression of LIF and MUC1 in the endometrial epithelium. We injected the uterine lumen of normal mice with high-glucose solution and cultured endometrial cells in high-glucose medium to mimic intrauterine hyperglycemia. Both studies provided compelling evidence that hyperglycemia could lead to diminished embryo implantation and dysregulated SGK1, LIF and MUC1. Additionally, through over-expression of SGK1 in vivo and in vitro, we found that enhanced SGK1 also decreased LIF expression, increased MUC1 expression, and attenuated embryo implantation rate. We further identified that hyperglycemia-activated SMAD2/3 might be responsible for the enhancement of SGK1 and verified directly the interaction between SMAD3 and corresponding SMAD binding elements within SGK1 promoter. Taken together, our study confirmed the association between diabetes-related hyperglycemia and endometrial receptivity defects. Hyperglycemia-induced SGK1 has a tremendous role in this pathological process, rendering it as an attractive therapeutic target for diabetes-related reproductive disorders. | Haiyan Xu Jingyi Li Luyang Jin Dan Zhang Bin Chen Xinmei Liu Xianhua Lin Yiting Huang Zhanghong Ke Juan Liu Lin Gao Jianzhong Sheng Hefeng Huang | 2022 | Science China(Life Sciences)2022，65，8： | 1 |
| 14 | One-step synthesis of the nanostructured AgI/BiOI composites with highly enhanced visible-light photoeatalytic performances显示文摘 | Cheng Hefeng Huang Aibiao Dai Ying | 2010 | Langmuir2010，26，9： | 1 |
| 15 | 显示文摘 | Chen Hefeng Huang Baibiao Zhang Xiaoyang | 2012 | Chem- ical Communications2012，48，1： | 1 |
| 16 | Decreased decidual epithelial sodium channel(ENaC) alpha and gamma subunits in recurrent pregnancy loss(RPL)显示文摘Dear Editor,Recurrent pregnancy loss (RPL) in early pregnancy is a devastating problem for couples who want to become parents and a difficult challenge for their physician.RPL is also referred to as recurrent miscarriage or habitual abortion.It is defined as two or more consecutive clinical miscarriages (CMs) before 20 weeks of gestation (Practice Committee of the American Society for Reproductive Medicine,2012). | Tiantian Yu Chun Feng Pingping Lv Gufeng Xu Lihua Hong Yimeng Xiong Xiaoyan Guo Tong Li Jianzhong Sheng Hefeng Huang Xinmei Liu | 2019 | Science China(Life Sciences)2019，62，7： | 1 |
| 17 | Recent advances and perspectives towards emission inventories of mobile sources:Compilation approaches,data acquisition methods,and case studies显示文摘In recent years,great efforts have been devoted to reducing emissions from mobile sources with the dramatic growth of motor vehicle and nonroad mobile source populations.Compilation of a mobile source emission inventory is conducive to the analysis of pollution emission characteristics and the formulation of emission reduction policies.This study summarizes the latest compilation approaches and data acquisition methods for mobile source emission inventories.For motor vehicles,a high-resolution emission inventory can be developed based on a bottom-up approach with a refined traffic flow model and real-world speed-coupled emission factors.The top-down approach has advantages when dealing with macroscale vehicle emission estimation without substantial traffic flow infrastructure.For nonroad mobile sources,nonroad machinery,inland river ships,locomotives,and civil aviation aircraft,a top-down approach based on fuel consumption or power is adopted.For ocean-going ships,a bottom-up approach based on automatic identification system(AIS)data is adopted.Three typical cases are studied,including emission reduction potential,a cost-benefit model,and marine shipping emission control.Outlooks and suggestions are given on future research directions for emission inventories for mobile sources:building localized emission models and factor databases,improving the dynamic updating capability of emission inventories,establishing a database of emission factors of unconventional pollutants and greenhouse gas from mobile sources,and establishing an urban high temporalspatial resolution volatile organic compound(VOC)evaporation emission inventory. | Han Jiang Hefeng Zhang Mingliang Fu Zhihui Huang Hong Ni Hang Yin Yan Ding | 2023 | Journal of Environmental Sciences2023，，1： | 0 |
| 18 | Developmental origins of adult diseases显示文摘The occurrence and mechanisms of developmental adult diseases have gradually attracted attention in recent years.Exposure of gametes and embryos to adverse environments,especially during plastic development,can alter the expression of certain tissue-specific genes,leading to increased susceptibility to certain diseases in adulthood,such as diabetes,cardiovascular disease,neuropsychiatric,and reproductive system diseases,etc.The occurrence of chronic disease in adulthood is partly due to genetic factors,and the remaining risk is partly due to environmental-dependent epigenetic information alteration,including DNA methylation,histone modifications,and noncoding RNAs.Changes in this epigenetic information potentially damage our health,which has also been supported by numerous epidemiological and animal studies in recent years.Environmental factors functionally affect embryo development through epimutation,transmitting diseases to offspring and even later generations.This review mainly elaborated on the concept of developmental origins of adult diseases,and revealed the epigenetic mechanisms underlying these events,discussed the theoretical basis for the prevention and treatment of related diseases. | Jiaying Mo Xuanqi Liu Yutong Huang Renke He Yu Zhang Hefeng Huang | 2022 | Medical Review2022，2，5： | 0 |
| 19 | Dietary adenosine supplementation improves placental angiogenesis in IUGR piglets by up-regulating adenosine A2a receptor显示文摘Abnormal placental angiogenesis is associated with the occurrence of intrauterine growth restriction(IUGR)in piglets,and effective treatment strategies against this occurrence remain to be explored.Adenosine has been reported to play an important role in angiogenesis,but its role in placental angio-genesis is still unknown.Here,we investigated the effect of dietary adenosine supplementation on IUGR occurrence in piglets by analyzing the role of adenosine in placental angiogenesis for Normal and IUGR piglets.Specifically,88 sows were allotted to 2 treatments(n=44)and fed a basal diet supplemented with 0%or 0.1%of adenosine from day 65 of gestation until farrowing,followed by collecting the placental samples of Normal and IUGR piglets,and recording their characteristics.The results showed that adenosine supplementation increased the mean birth weight of piglets(P<0.05)and placental efficiency(P<0.05),while decreasing the IUGR piglet rate(P<0.05).Expectedly,the placenta for IUGR neonates showed a down-regulated vascular density(P<0.05)and angiogenesis as evidenced by the expression level of vascular cell adhesion molecule-1(VCAM1)(P<0.05).Notably,dietary adenosine supplementation promoted angiogenesis(P<0.05)both in the Normal and IUGR placenta.More importantly,the expression level of adenosine A2a receptor(ADORA2A)was lower(P<0.05)in the IUGR placenta than in Normal placenta,whereas adenosine treatment could significantly increase ADORA2A expression,and also had an interaction effect between factors IUGR and Ado.Collectively,placentae for IUGR piglets showed impaired angiogenesis and down-regulated expression level of ADORA2A,while dietary adenosine supplementation could activate ADORA2A expression,improve the placental angio-genesis,and ultimately decrease the occurrence of IUGR in piglets. | Zifang Wu Jiawei Nie Deyuan Wu Shuangbo Huang Jianzhao Chen Huajin Liang Xiangyu Hao Li Feng Hefeng Luo Chengquan Tan | 2023 | Animal Nutrition2023，，2： | 0 |
| 20 | An alternative splicing variant of mineralocorticoid receptor discovered in preeclampsia tissues and its effect on endothelial dysfunction显示文摘The pathophysiology of preeclampsia(PE)remains unclear.PE spiral artery remodeling dysfunction and PE offspring cardiovascular future development has been a worldwide concern.We collected placental and umbilical artery samples from normotensive and PE pregnancies.Mineralocorticoid receptor(MR)and its alternative splicing variant(ASV)expression and their biological effects on PE were examined.An MR ASV was found to be highly expressed in all PE samples and slightly expressed in about half of the normotensive samples(umbilical artery,~57.58%;placenta,~36.84%).The MR ASV expression was positively associated with blood pressure in both groups.The MR ASV protein changed the aldosterone-induced expression pattern of MR target genes related to ion exchanges and cell signaling pathways.The MR ASV can also impair the proliferation,migration,and tube formation ability of endothelial cells.These findings indicate that MR ASV in PE placenta plays a pathogenic role in PE pathophysiology,especially in endothelial dysfunction,and the existence of the MR ASV in PE umbilical artery provides a new direction in the study of PE offspring with increased risk of cardiovascular diseases. | Mengxi Guo Chengliang Zhou Gufeng Xu Lin Tang Yechun Ruan Ying Yu Xianhua Lin Dandan Wu Hao Chen Priscilla Yu Luyang Jin Yinyu Wang Yimei Wu Kamran Ullah Tanzil Ur Rahman Xinmei Liu Jianzhong Sheng Hsiao-Chang Chan Hefeng Huang | 2020 | Science China(Life Sciences)2020，63，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员