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| 1 | Effects of AT1 receptor antagonist,Iosartan,on rat hepatic fibrosis induced by CCl_4显示文摘AIM To investigate effect of losartan,an AT1receptor antagonist,on hepatic fibrosis induced byCCl;and to determine whether or not AT1receptors are expressed on hepatic stellate cells,METHODS AND RESULTS Fifty male Sprague-Dawley rats,weighing(180±20)g,wererandomized into five groups(control group,modelgroup,and three losartan treated groups),inwhich all rats were given the subcutaneousinjection of 40% CCl4(every 3 days for 6 weeks)except for rats of control group.Rats of losartan-treated groups were treated with losartan(20 mg/kg,10 mg/kg,5 mg/kg,daily gavage),After 6weeks liver tissue and serum samples of all ratswere examined.Serum hyaluronic acid(HA),procollagen typeⅢ(PCⅢ)were detected byradioimmunoassays,van Giesion collagen stainingwas used to evaluate the extracellular matrix of ratswith liver fibrosis.The expression of AT1receptors,transforming growth factor-beta(TGF-β),and alpha-smooth muscle actin(a-SMA)inliver tissue were determined byimmunohistochemical techniques.Compared withmodel group,serum ALT and AST of losartan-treated groups were significantly reduced(t=4.20,P<0.01 and t=4.57,P<0.01).Serum HAand PCⅢalso had significant differences(t=3.53,P<0.01 and t=2.20,P<0.05).Thedegree of fibrosis was improved by losartan and correlated with the expressions of AT1 receptors,TGF-β,and α-SMA in liver tissue.CONCLUSION AT1 receptor antagonist,losartan,could limit the progression of the hepatic fibrosisinduced by CCl4.The mechanism may be related tothe decrease in the expression of AT1 receptorsand TGF-β,ameliorating the injury of hepatocytes;activation of local renin-angiotensin system mightrelate to hepatic fibrosis;and during progressionof fibrosis,activated hepatic stellate cells mightexpress AT1 receptors. | Hong Shan Wei Ding Guo Li Han Ming Lu Yu Tao Zhan Zhi Rong Wang Xin Huang Jing Zhang Ji Lin Cheng Qin Fang Xu Department of Gastroenterology,Xinhua Hospital,Shanghai Second Medical University,Shanghai 200092,China | 2000 | World Journal of Gastroenterology2000，6，4： | 42 |
| 2 | Trends in Lipids Level and Dyslipidemia among Chinese Adults, 2002-2015显示文摘Objective To investigate the trends of lipid profiles and dyslipidemia among Chinese adults from 2002 to 2015.Methods Data were collected from three nationally representative cross-sectional surveys.Fasting venous blood samples were collected and serum lipids were tested by biochemical analysis and enzymatic determination.Lipid levels and the prevalence of dyslipidemia among adults were analyzed with complex sampling weighting adjustment for age and gender.Results The weighted means of TC, TG, and LDL-c significantly increased linearly from 3.93, 1.12, and 2.12 mmol/L in 2002 to 4.59, 1.41, and 2.78 mmol/L in 2010 and then to 4.63, 1.47, and 2.87 mmol/L in 2015, respectively;by contrast, HDL-c levels decreased significantly from 1.30 mmol/L to 1.26 mmol/L over the same period.Similar trends in mean non-HDL-c and lipid-related ratios were observed.The weighted dyslipidemia prevalence linearly increased;in particular, hypercholesterolemia increased from 1.6% to 5.6% and then to 5.8%, hypertriglyceridemia increased from 5.7% to 13.6% and then to 15.0%, low HDL-c increased from 18.8% to 35.5% and then to 24.9%, and high LDL-c increased from 1.3% to 5.6% and then to 7.2%(P for trend <0.001).Conclusion Dyslipidemia increased among Chinese adults from 2002 to 2015.Development of a comprehensive strategy to decrease lipid levels in this population is urgently required. | SONG Peng Kun MAN Qing Qing LI Hong PANG Shao Jie JIA Shan Shan LI Yu Qian HE Li ZHAO Wen Hua ZHANG Jian | 2019 | Biomedical and Environmental Sciences2019，32，8： | 45 |
| 3 | Chemotherapy and target therapy for hepatocellular carcinoma:New advances and challenges显示文摘Primary liver cancer is one of the commonest causes of death.Hepatocellular carcinoma(HCC) accounts for 90% of primary liver cancers.For patients with unresectable or metastatic HCC,conventional chemotherapy is of limited or no benefit.Sorafenib is the only systemic treatment to demonstrate a statistically significant but modest overall survival benefit,leading to an era of targeted agents.Many clinical trials of targeted drugs have been carried out with many more in progress.Some drugs like PTK787 showed potential benefits in the treatment of HCC.Despite these promising breakthroughs,patients with HCC still have a dismal prognosis.Recently,both a phase Ⅲ trial of everolimus and a phase Ⅱ clinical trial of trebananib failed to demonstrate effective antitumor activity in advanced HCC.Sorafenib still plays a pivotal role in advanced HCC,leading to further explorations to exert its maximum efficacy.Combinations targeted with chemotherapy or transarterial chemoembolization is now being tested and might bring about advances.New targeted agents such as mammalian target of rapamycin inhibitors are under investigation,as well as further exploration of the mechanism of hepatocarcinogenesis. | Gan-Lu Deng Shan Zeng Hong Shen | 2015 | World Journal of Hepatology2015，7，5： | 30 |
| 4 | The regulatory role of AT 1 receptor on activated HSCs in hepat,c fibrogenesis,effects of RAS inhibitors on hepatic fibrosis induced by CCl_4显示文摘AIM To assess the effect of ACE inhibitor andAng Ⅱ type Ⅰ(AT1)receptor antagonist inpreventing hepatic fibrosis caused by CCl4administration in rats;to investigate whether ornot there are expression of AT 1 receptors onhepatic stellate cells;and to observe the effectof Ang Ⅱ on proliferation and ECM synthesis ofcultured HSCs.METHODS Studies were conducted in maleSprague-Dawley rats.Except for thehepatofibrotic model group and the controlgroup,in three treated groups,either enalapril(5 mg/kg),or Iosartan(10 mg/kg),or enalapril+Iosartan were given to the fibrotic rats bydaily gavage,and saline vehicle was given tomodel and normal control rats.After 6 weeks,liver fibrosis was assessed directly by hepaticmorphometric analysis,which has beenconsidered the gold standard for thequantification of fibrosis.The expressions of AT1 receptors and(α-mooth muscle actin,α-SMA)in liver tissue or isolated hepatic stellate cells(HSCs)were detected by immunohistochemicaltechniques.The effect of Ang Ⅱ on HSCproliferation was determined by MTT method.Effect of Ang Ⅱ on collagen synthesis of HSCswas determined by 3H-proline incorporation.RESULTS Contrasted to the fibrosis in rats ofthe model group,groups of rats treated with either enalapril or Iosartan,or a combination oftwo drugs showed a limited expansion of theinterstitium(4.23±3.70 vs 11.22±4.79,P<0.05),but no difference was observedamong three treated groups(5.38±3.43,4.96±2.96,4.23±2.70,P>0.05).Expression of AT 1receptors was found in fibrotic interstitium offibrotic rats,whereas in normal control rats theywere limited to vasculature only to a very slightdegree.AT 1 receptors were also expressed onactivated HSCs in the culture.At concentrationsfrom 10-9to 10-5mol/L,Ang Ⅱ stimulated HSCproliferation in culture in a dose-dependentmanner.Increasing Ang Ⅱ concentrationsproduced corresponding increases in 3H-prolineincorporation.Differences among groups were significant.CONCLUSION Angiotensin-converting enzyme inhibitors and AT I blocker may slow the progression of hepatic fibrosis; activated HSCs express AT 1 receptors, and Ang Ⅱ can stimulate the proliferation and collagen synthesis of HSCs in a dose-dependent manner; and activation of RAS may be related to hepatic fibrogenesis induced by CCI4. | Hong Shan Wei Han Ming Lu Ding Guo Li Yu Tao Zhan Zhi Rong Wang Xin Huang Ji Lin Cheng Qin Fang Xu Department of Gastroenterology,Xinhua Hospital,Shanghai Second Medical University,Shanghai 200092,China | 2000 | World Journal of Gastroenterology2000，6，6： | 27 |
| 5 | Gender and metabolic differences of gallstone diseases显示文摘AIM:To investigate the risk factors for gallstone disease in the general population of Chengdu,China. METHODS:This study was conducted at the West China Hospital.Subjects who received a physical examination at this hospital between January and December 2007 were included.Body mass index,blood pressure,fasting plasma glucose,serum lipid and lipoproteins concentrations were analyzed.Gallstone disease was diagnosed by ultrasound or on the basis of a history of cholecystectomy because of gallstone disease.Unconditional logistic regression analysis was used to investigate the risk factors for gallstone disease,and the Chi-square test was used to analyze differences in the incidence of metabolic disorders between subjects with and without gallstone disease. RESULTS:A total of 3573 people were included, 10.7%(384/3573)of whom had gallstone diseases. Multiple logistic regression analysis indicated that the incidence of gallstone disease in subjects aged 40-64 or≥65 years was significantly different from that in those aged 18-39 years(P<0.05);the incidence was higher in women than in men(P<0.05).In men,a high level of fasting plasma glucose was obvious in gallstone disease(P<0.05),and in women, hypertriglyceridemia or obesity were significant in gallstone disease(P<0.05). | Hui Sun Hong Tang Shan Jiang Li Zeng En-Qiang Chen Tao-You Zhou You-Juan Wang | 2009 | World Journal of Gastroenterology2009，15，15： | 25 |
| 6 | DNA-based vaccination induces humoral and cellular immune responses against hepatitis B virus surface antigen in mice without activation of Cmyc显示文摘AIM To develop a safe and effective DNAvaccine for inducing humoral and cellularimmunological responses against hepatitis Bvirus surface antigen(HBsAg).METHODS BALB/c mice were inoculated withNV-HB/s,a recombinant plasmid that had beeninserted S gene of hepatitis B virus genome andcould express HBsAg in eukaryotes.HBsAgexpression was measured by ABC immunohis-tochemical assay,generation of anti-HBs byELISA and cytotoxic T lymphocyte(CTL),byMTT method,existence of vaccine DNA bySouthern blot hybridization and activation ofoncogene C-myc by in situ hybridization,RESULTS With NV-HB/s vaccination byintramuscular injection,anti-HBs was initiallypositive 2 weeks after inoculation while all micetested were HBsAg positive in the muscles.Thetiters and seroconversion rate of anti-HBs weresteadily increasing as time went on and weredose-dependent.All the mice inoculated with100 μg NV-HB/s were anti-HBs positive onemonth after inoculation,the titer was 1:1024 ormore.The humoral immune response was similarinduced by either intramuscular or intradermalinjection.CTL activities were much stronger(45.26%)in NV-HB/s DNA immunized mice as compared with those(only 6%)in plasma-derived HBsAg vaccine immunized mice.Twomonths after inoculation,all muscle sampleswere positive by Southern-blot hybridization forNV.HB/s DNA detection,but decreased to 25%and all were undetectable by in situhybridization after 6 months.No oncogene C-myc activation was found in the muscle ofinoculation site.CONCLUSION NV-HB/s could generatehumoral and cellular immunological responsesagainst HBsAg that had been safely expressed insitu by NV-HB/s vaccination. | Lian San Zhao Shan Qin Tao You Zhou Hong Tang Li Liu Bing Jun Lei | 2000 | World Journal of Gastroenterology2000，6，2： | 24 |
| 7 | Special Section:SARS-CoV-2 Preliminary evidence from a multicenter prospective observational study of the safety and efficacy of chloroquine for the treatment of COVID-19显示文摘Effective therapies are urgently needed for the SARS-CoV-2 pandemic.Chloroquine has been proved to have antiviral effect against coronavirus in vitro.In this study,we aimed to assess the efficacy and safety of chloroquine with different doses in COVID-19.In this multicenter prospective observational study,we enrolled patients older than 18 years old with confirmed SARS-CoV-2 infection excluding critical cases from 12 hospitals in Guangdong and Hubei Provinces.Eligible patients received chloroquinephosphate s00 mg,orally,once(half dose)or twice(full dose)daily.Patients treated with non-chloroquine therapy were included as historical controls.The primary endpoint is the time to undetectable viral RNA.Secondary outcomes include the proportion of patients with undetectable viral RNA by day 10 and 14,hospitalization time,duration of fever,and adverse events.A total of 197 patients completed chloroquine treatment,and 176patients were included as historical controls.The median time to achieve an undetectable viral RNA was shorter in chloroquine than in non-chloroquine(absolute difference in medians-6.0 days;95%CI-6.0 to—4.0).The duration of fever is shorter in chloroquine(geometric mean ratio 0.6;95%CIO.5 to 0.8).No serious adverse events were observed in the chloroquine group.Patients treated with half dose experienced lower rate of adverse events than with full dose.Although randomized trials are needed for further evaluation,this study provides evidence for safety and efficacy of chloroquine in COVID-19 and suggests that chloroquine can be a cost-effective therapy for combating the COVID-19 pandemic. | Mingxing Huang Man Li Fei Xiao Pengfei Pang Jiabi Liang Tiantian Tang Shaoxuan Liu Binghui Chen Jingxian Shu Yingying You Yang Li Meiwen Tang Jianhui Zhou Guanmin Jiang Jingfen Xiang Wenxin Hong Songmei He Zhaoqin Wang Jianhua Feng Changqing Lin Yinong Ye Zhilong Wu Yaocai Li Bei Zhong Ruilin Sun Zhongsi Hong Jing Liu Huili Chen Xiaohua Wang Zhonghe Li Duanqing Pei Lin Tian Jinyu Xia Shanping Jiang Nanshan Zhong Hong Shan | 2020 | National Science Review2020，7，9： | 20 |
| 8 | Puerarin enhances superoxide dismutase activity and inhibits RAGE and VEGF expression in retinas of STZ-induced early diabetic rats显示文摘Objective:To investigate the effects of puerarin on the activity of superoxide dismutase(SOD), and expressions of advanced glycation end-product(AGE) receptor(RAGE) and vascular endothelial growth factor(VEGF) in retinas of streptozotocin(STZ)-induced early diabetic rats. Methods:Diabetic rat models were established by inducing diabetes via intra-peritoneal injection of STZ.Rats were randomly divided into normal(control),diabetic(DM),and DM+ puerarin groups.After intra-gastric administration of puerarin(500 mg/kg/day for 4 weeks),levels of SOD and malondialdehyde(MDA) were determined in serum and retina.mRNA and protein expression levels of RAGE and VEGF in retinas were determined by real-lime polymerase chain reaction(RT-PCR)(mRNA) and Western blot analysis(protein levels).Results:There was significantly lower SOD activity and significantly higher MDA in serum and retinas of the DM group compared with the two other groups(P<0.05).After treatment with puerarin,SOD activity increased and MDA content decreased in this group(P<0.05).mRNA and protein expression levels of RACE and VECF in the DM group were significantly higher than those of the other groups (P<0.05),and decreased after puerarin treatment(P<0.05).Conclusions:Puerarin is able to enhance SOD activity,and inhibit RAGE and VEGF expressions in retinas of STZ-induced early diabetic ruts. | Fang Chen Hong-Quan Zhang Jun Zhu Kai-Yang Liu Hong Cheng Guo-Li Li Shan Xu Wei-Hong Lv Zheng-Gao Xie | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，11： | 16 |
| 9 | Recurrent gain-of-function USP8 mutations in Cushing's disease显示文摘库欣的疾病,引起过量皮质醇生产的也已知的同样促肾上腺皮质的荷尔蒙(ACTH )-secreting 垂体腺瘤(舞步) ,占多达 85%corticotrophin 依赖的库欣的症候群盒子。然而,在这疾病的基因改变是不清楚的。这里,我们执行了从 12 秘密 ACTH 舞步导出并且匹配血样品的 DNA 定序的 whole-exome,它在候选人基因揭示了体的变化的三种类型, USP8 (编码 ubiquitin 特定的朊酶 8 ) ,专门在 exon 14 在 12 秘密 ACTH 舞步中的 8 个。我们进一步评估了体的 USP8 变化在另外由定序的 Sanger 的 258 舞步。进一步定序指向在 108 秘密 ACTH 舞步(62.04%) 中的 67 个识别了 USP8 变体的 17 种类型的一个总数。然而,任何一个都没在舞步的另外的类型这些变化被检测(n = 150 ) 。这些变化在 USP8 的 14-3-3 绑定主题以内聚集并且破坏在 USP8 和 14-3-3 蛋白质之间的相互作用,导致保护 EGFR 免受 lysosomal 降级的伤害的一个提高的能力。因此,有 EGFR 表示,提高的 EGFR 蛋白质丰富和 mRNA 表示的更高的发生 POMC 铺平的变异的 USP8 显示的舞步,它编码 ACTH 的先锋。有变异的 USP8 的舞步在尺寸是显著地更小的并且比野类型的舞步有更高的 ACTH 生产。在里面通过手术 resected 主要变异 USP8 的肿瘤房间, USP8 击倒或堵住的 EGFR 有效地稀释 ACTH 分泌物。一起拿,体的 gain-of-function USP8 变化是普通的并且在库欣的疾病贡献 ACTH 生产过剩。USP8 或 EGFR 的抑制为对待变异 USP8 的 corticotrophin 腺瘤是有希望的。我们的学习在库欣的疾病加亮潜在地功能的变异的基因并且提供卓见进这疾病的治疗学。 | Zeng-Yi Ma Zhi-Jian Song Jian-Hua Chen Yong-Fei Wang Shi-Qi Li Liang-Fu Zhou Ying Mao Yi-Ming Li Rong-Gui Hu Zhao-Yun Zhang Hong-Ying Ye Ming Shen Xue-Fei Shou Zhi-Qiang Li Hong Peng Qing-Zhong Wang Dai-Zhan Zhou Xiao-Lan Qin Jue Ji Jie Zheng Hong Chen Yin Wang Dao-Ying Geng Wei-Jun Tang Chao-Wei Fu Zhi-Feng Shi Yi-Chao Zhang Zhao Ye Wen-Qiang He Qi-Lin Zhang Qi-Sheng Tang Rong Xie Jia-Wei Shen Zu-Jia Wen Juan Zhou Tao Wang Shan Huang Hui-Jia Qiu Ni-Dan Qiao Yi Zhang Li Pan Wei-Min Bao Ying-Chao Liu Chuan-Xin Huang Yong-Yong Shi Yao Zhao | 2015 | Cell Research2015，25，3： | 16 |
| 10 | Double-balloon enteroscopy for obscure gastrointestinal bleeding: A single center experience in China显示文摘AIM: To evaluate the diagnostic value of double-balloon enteroscopy (DBE) for obscure gastrointestinal bleeding (OGIB). METHODS: The data about 75 OGIB patients who underwent DBE in January 2007-June 2009 in our hospital were retrospectively analyzed. RESULTS: DBE was successfully performed in all 75 patients without complication. Of the 75 patients, 44 (58.7%) had positive DBE findings, 22 had negative DBE findings but had potential bleeding at surgery and capsule endoscopy, etc . These 66 patients were finally diagnosed as OGIB which was most commonly caused by small bowel tumor (28.0%), angiodysplasia (18.7%) and Crohn’s disease (10.7%). Lesions occurred more frequently in proximal small bowel than in distal small bowel (49.3% vs 33.3%, P = 0.047). CONCLUSION: DBE is a safe, effective and accurate procedure for the diagnosis of OGIB. | Chen, Li-Hua Chen, Wen-Guo Cao, Hai-Jun Zhang, Hong Shan, Guo-Dong Li, Lin Zhang, Bing-Ling Xu, Cheng-Fu Ding, Kai-Li Fang, Ying Cheng, Ying Wu, Chen-Jiao Xu, Guo-Qiang | 2010 | World Journal of Gastroenterology2010，16，13： | 15 |
| 11 | Congenital coronary artery fistulas: dual-source CT findings from consecutive 6624 patients with suspected or confirmed coronary artery disease显示文摘背景冠的动脉管( CAF )无意中经常是稀罕先天的反常与冠的动脉 disease.Clinical 在病人发现 CAF 的诊断是困难的由于无症状或特定的symptoms.Dual来源的缺乏计算了断层摄影术( DSCT )可能是为学习试图回顾地总结 CAF 的成像特征的 CAFs.The 的一个有用诊断工具在检测从的 48 个 CAF 病人在 DSCT 描出连续 6624 个病人与怀疑了或证实冠的动脉 | YUN Hong ZENG Meng-su YANG Shan JIN Hang YANG Xue | 2011 | Chinese Medical Journal2011，，24： | 15 |
| 12 | Small molecule inhibitors reveal allosteric regulation of USP14 via steric blockade显示文摘The ubiquitin system is important for drug discovery,and the discovery of selective small-molecule inhibitors of deubiquitinating enzymes (DUBs) remains an active yet extremely challenging task. With a few exceptions,previously developed inhibitors have been found to bind the evolutionarily conserved catalytic centers of DUBs,resulting in poor selectivity. The small molecule IU1 was the first-ever specific inhibitor identified and exhibited surprisingly excellent selectivity for USP14 over other DUBs. However,the molecular mechanism for this selectivity was elusive. Herein,we report the high-resolution co-crystal structures of the catalytic domain of USP14 bound to IU1 and three IU1 derivatives. All the structures of these complexes indicate that IU1 and its analogs bind to a previously unknown steric binding site in USP14,thus blocking the access of the C-terminus of ubiquitin to the active site of USP14 and abrogating USP14 activity. Importantly,this steric site in USP14 is very unique,as suggested by structural alignments of USP14 with several known DUB X-ray structures. These results,in conjunction with biochemical characterization,Indicate a coherent steric blockade mechanism for U5P14 inhibition by compounds of the IU series. In light of the recent report of steric blockade of USP7 by FT671,this work suggests a potential generally applicable allosteric mechanism for the regulation of DUBs via steric blockade,as showcased by our discovery of IU1-248 which is 10-fold more potent than IU1. | Yiwei Wang Yuxuan Jiang Shan Ding Jiawang Li Ningjing Song Yujing Ren Danning Hong Cai Wu Bin Li Feng Wang Wei He Jiawei Wang Ziqing Mei | 2018 | Cell Research2018，28，12： | 15 |
| 13 | Liver targeting and the delayed drug release of the nanoparticles of adriamycin polybutylcyanoacrylate in mice显示文摘指向药交货系统的背景肝能改进药品效果并且减轻在带治疗学的药的肝 diseases.Nanoparticles 的治疗的药当前在有大临床的意义的热调查下面的化疗的 cytotoxicity。这研究被瞄准经由侧面的尾巴静脉在注射以后在老鼠身体调查 theadriamycin polybutylcyanoacrylate nanoparticle (ADM-PBCA-NP ) 的不同组织分发,并且学习在正常老鼠肝上在不同直径指向 ADM-PBCA-NP 的效果的肝。180 只 Kunming 老鼠随机在各个带着 30 只老鼠被划分成 6groups 的方法组织(在不同直径范围的 ADM-PBCA-NP 的 5 个处理组,非结合的免费 adriamycin 注射作为控制组被采用) 。结合的任何一个或免费 adriamycin 的单个剂量等于身体重量的 2 mg/kg 经由尾巴静脉被交付。在每条小道的五个鼠标在 5 点被牺牲, 15, 30 分钟, 1, 5 和 12 小时帖子注射分别地。adriamycin 集中在分别地镇定肝,肾,怒气,心,肺和血浆与荧光察觉者用高效液体层析被表明。结果与控制组,相比, adriamycin 几乎没在处理组的心肌肉被检测(P <
0.05 ) 。结合 nanoparticle 的 adriamycin 从肾织物快速被清理。在试验性的组的鼠标肝和怒气的 adriamycin 集中在控制组比那显著地高,除了有 nanoparticles 直径的组(22.3 ± 6.2 ) nm (P <
0.05 ) 。ADM-PBCA-NP 在(101.0 ± 20.3 ) nm 直径有最高的肝分发,并且在肝的第二最高的 adriamycindistribution 是组(143.0 ± 2 3.5 ) nm 直径(P <
0.05 ) 。而且,在试验性的组在察觉时期期间在肝慢慢地释放的 adriamycinwas。ADM-PBCA-NPin (22.3 ± 6 .2 ) nm 直径没在肝,肾,心,怒气,和肺的织物被散布。在 100-150 nm 直径范围的结论 ADM-PBCA-NP 有最好的肝与慢药版本的一个特征指向。它也减少在心,肾和肺的药分发。在肝癌症的治疗, polybutylcyanoacrylate nanoparticles 系统有一个好肝指向能力,它增加反癌症活动并且显著地减少 adriamycin 的毒性。 | SHEN Liang-fang ZHANG Yang-de SHEN Hai-ju ZENG Shan WANG Xin WANG Cheng LE Yuan SHEN Hong | 2006 | Chinese Medical Journal2006，，15： | 15 |
| 14 | Clinical significance of multislice spiral CT scans in hepatic veins occlusion in Budd-Chiari syndrome显示文摘有肝的静脉吸藏(HVBCS ) 的背景 Budd-Chiari 症候群能导致严重的门高血压和肝损坏。我们回顾地分析了 HVBCS 的肝的 CT 特征并且在它的 diagnosis.Methods 评估了 CT 考试和 CT angiography (CTA ) 的 triphasic 改进的实用性有 HVBCS 的 25 个盒子,由数字减法 angiography (DSA ) 证实了,在 DSA 前在一个星期以内收到了 triphasic 改进 CT 扫描。相关的血容器的 CTA 图象与最大的紧张设计被重建,显示的体积并且倾斜与 DSA ,横向的 CT 的察觉率和 CTA 图象相比重新格式化 techniques.Results 因为反常肝的静脉是81.7%( 58/71 )并且95.8%( 68/71 )( 2=7.044 , P=0.008 ),为膜的阻塞是47.4%( 9/19 )并且84.2%( 16/19 )分别地( 2 =5.729 , P=0.017 )因为部分阻塞是88.0%( 22/25 )并且100%( 25/25 )分别地( 2=1.418 , P=0.234 )。为肝的静脉狭窄的察觉率是有每个方法的 100% 。弥漫的肝肿大在尖锐阶段在所有 6 个盒子中被发现并且在长期的阶段的 19 个盒子中的 3 个没有专利 intrahepatic 担保,有三根肝的静脉的严重阻塞。在长期的阶段的另外的 16 个盒子有 hepatatrophia 到与妨碍的肝的静脉有关的不同程度。都在尖锐阶段并且 15 开始在有尾的脑叶和 perihilar 区域在长期的阶段介绍了典型补缀的改进并且与时间扩大了延期。在所有情况中,有萎缩,坏死和拥挤的实质区域表明了更低、以后的改进。在所有部分,它有正常改进,至少一获流出专利肝的静脉,附件肝的静脉或并行的容器被检测。由 multislice 螺线 CT 的结论动态改进 CT 检查能不仅由 CTA 技术改进 HVBCS 的诊断,而且能 noninvasively 提供解剖信息并且揭示损坏到肝的实质。 | MENG Xiao-chun ZHU Kang-shun QIN Jie ZHANG Jian-sheng WANG Xiao-hong ZOU Yan ZHANG Ya-qin SHAN Hong | 2007 | Chinese Medical Journal2007，，2： | 15 |
| 15 | Wavelet Analysis and Nonparametric Test for Climate Change in Tarim River Basin of Xinjiang During 1959-2006显示文摘Using wavelet analysis,regression analysis and the Mann-Kendall test,this paper analyzed time-series(1959-2006) weather data from 23 meteorological stations in an attempt to characterize the climate change in the Tarim River Basin of Xinjiang Uygur Autonomous Region,China.Major findings are as follows:1) In the 48-year study period,average annual temperature,annual precipitation and average annual relative humidity all presented nonlinear trends.2) At the 16-year time scale,all three climate indices unanimously showed a rather flat before 1964 and a detectable pickup thereafter.At the 8-year time scale,an S-shaped nonlinear and uprising trend was revealed with slight fluctuations in the entire process for all three indices.Incidentally,they all showed similar pattern of a slight increase before 1980 and a noticeable up-swing afterwards.The 4-year time scale provided a highly fluctuating pattern of periodical oscillations and spiral increases.3) Average annual relative humidity presented a negative correlation with average annual temperature and a positive correlation with annual precipitation at each time scale,which revealed a close dynamic relationship among them at the confidence level of 0.001.4) The Mann-Kendall test at the 0.05 confidence level demonstrated that the climate warming trend,as represented by the rising average annual temperature,was remarkable,but the climate wetting trend,as indicated by the rising annual precipitation and average annual relative humidity,was not obvious. | XU Jianhua CHEN Yaning LI Weihong JI Minhe DONG Shan HONG Yulian | 2009 | Chinese Geographical Science2009，19，4： | 14 |
| 16 | Expression changes of activin A in the development of hepatic fibrosis显示文摘AIM To examine the expression of activin A, amember of the transforming growth factor (TGF-β) superfamily, recently has been reported to beoverexpressed in liver cirrhosis, in the course ofcarbon tetrachloride-induced rat hepaticfibrosis.METHODS Hepatic fibrosis was induced in ratsby subcutaneous injections of 40% carbontetrachloride oily solution for a period of 1 to 7weeks. At the end of 1, 2, 3, 4, 5, 6 and 7 weeksafter carbon tetrachloride injections, the ratswere killed in group (6- 10 rats each time) forstudy. The activin A messenger RNA expressionand its protein localization were assessed bysemi-quantitative reverse transcriptionpolymerase chain reaction ( RT-PCR ) andimmunohistochemistry.RESULTS The normal rat liver expressedactivin A mRNA and protein, and its expressionwas transiently decreased and becameundetectable after carbon tetrachlorideinjections for 2 or 3 weeks and then increasedgradually. After injection of carbon tetrachloridefor 6 and 7 weeks, activin A mRNA and proteinexpressions were significantly enchanced in ratliver. Compared with that of the normal ratliver. Activin A mRNA expression levels in ratsreceiving carbon tetrachloride injections for 6and 7 weeks were 1.6 and 2.2 times that of thosein normal rat liver respectively (0.456±0.094 vs0.286± 0.0670, P<0.01; 0.620± 0.134 vs 0.286±± 0.0670, P<0.01). Immunohistochemistryshowed that activin A expressed in hepatocytesof normal liver, and its expression wasdecreased in rats receiving carbon tetrachloridefor 2 or 3 weeks. Compared with normal liver,activin A expression distribution mode changedin fibrotic liver, being increased significantly inhepatocytes around fibrotic areas.CONCLUSION Activin A expression wasincreased in latestage of hepatic fibrosis, andthis may be involved in hepatic fibrosisformation in this period. | Xin Huang1 Ding Guo Li1 Zhi Rong Wang1 Hong Shan Wei1 Ji Lin Cheng1 Yu Tao Zhan1 Xin Zhou1 Qin Fang Xu1 Xin Li2 Han Ming Lu1 1Xinhua Hospital, Shanghai Second Medical University, Shanghai 200092, China2Ninth Hospital. Shanghai Second Medical University, Shanghai 200011. China | 2001 | World Journal of Gastroenterology2001，7，1： | 12 |
| 17 | Overexpression of DNA methyltransferase 1 and its biological significance in primary hepatocellular carcinoma显示文摘AIM:To explore the relationship between DNA methyltransferase 1 (DNMT1) and hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) and its biological significance in primary HCC.METHODS: We carried out an immunohistochemical examination of DNMT1 in both HCC and paired non-neoplastic liver tissues from Chinese subjects. DNMT1 mRNA was further examined in HCC cell lines by real-time PCR. We inhibited DNMT1 using siRNA and detected the effect of depletion of DNMT1 on cell proliferation ability and cell apoptosis in the HCC cell line SMMC-7721.RESULTS: DNMT1 protein expression was increased in HCCs compared to histologically normal non-neoplastic liver tissues and the incidence of DNMT1 immunoreactivity in HCCs correlated signifi cantly with poor tumor differentiation (P=0.014). There were more cases with DNMT1 overexpression in HCC with HBV (42.85%) than in HCC without HBV (28.57%). However, no signif icant difference in DNMT1 expression was found in HBV-positive and HBV-negative cases in the Chinese HCC group. There was a trend that DNMT1 RNA expression increased more in HCC cell lines than in pericarcinoma cell lines and normal liver cell lines. In addition, we inhibited DNMT1 using siRNA in the SMMC-7721 HCC cell line and found depletion of DNMT1 suppressed cells growth independent of expression of proliferating cell nuclear antigen (PCNA), even in HCC cell lines where DNMT1 was stably decreased.CONCLUSION: The f indings implied that DNMT1 plays a key role in HBV-related hepatocellular tumorigenesis. Depletion of DNMT1 mediates growth suppression in SMMC-7721 cells. | Hong Fan Zhu-Jiang Zhao Jian Cheng Xian-Wei Su Qing-Xiang Wu Yun-Feng Shan | 2009 | World Journal of Gastroenterology2009，15，16： | 11 |
| 18 | Sequencing of p53 mutation in established human hepatocellular carcinoma cell line of HHC4 and HHC15 in nude mice显示文摘INTRODUCTIONAlthoughtheetiopathologyofhumanhepatocelularcarcinoma(hHCC)isstilunknown,alotofevidencestronglysuggestedthatinfec... | YANG Shan Min, ZHOU Hong, CHEN Rui Chuan, WANG Yu Fang, CHEN Fu, ZHANG Chang Gong, ZHEN Yun, YAN Jiang Hua and SU Jin Hua | 1998 | World Journal of Gastroenterology1998，4，6： | 11 |
| 19 | Metals in sediment/pore water in Chaohu Lake:Distribution,trends and flux显示文摘Nine metals,Cd,Cu,Ni,Pb,As,Cr,Zn,Fe,and Mn in sediment and pore water from 57 sampling sites in Chaohu Lake(Anhui Province,China)were analyzed for spatial distribution,temporal trends and diffuse flux in 2010.Metals in the surface sediment were generally the highest in the western lake center and Nanfei-Dianbu River estuary,with another higher area of As,Fe,and Mn occurring in the Qiyang River estuary.Metal contamination assessment using the New York sediment screening criteria showed that the sediment was severely contaminated in 44% of the area with Mn,20% with Zn,16% with Fe,14% with As,and 6% with Cr and Ni.An increasing trend of toxic metals(Cd,Cu,Ni,Pb,As,Cr,Zn)and Mn with depth was shown in the western lake.Compared with metal content data from the sediment survey conducted in 1980s,the metal content of surface sediment in 2010 was 2.0 times that in the 1980s for Cr,Cu,Zn,and As in the western lake,and less than 1.5 times higher for most of the metals in the eastern lake.Among the metals,only Mn and As had a widespread positive diffuse flux from the pore water to overlying water across the whole lake.The estimated flux in the whole lake was on average 3.36mg/(m2·day)for Mn and 0.08mg/(m2·day)for As,which indicated a daily increase of 0.93μg/L for Mn and 0.02μg/L for As in surface water.The increasing concentration of metals in the sediment and the flux of metals from pore water to overlying water by diffusion and other physical processes should not be ignored for drinking-water sources. | Shengfang Wen Baoqing Shan Hong Zhang | 2012 | Journal of Environmental Sciences2012，24，12： | 11 |
| 20 | Double-balloon enteroscopy in small bowel tumors: A Chinese single-center study显示文摘AIM: To analyze the clinical characteristics of small bowel tumors detected by double-balloon enteroscopy (DBE) and to evaluate the diagnostic value of DBE in tumors. METHODS: Four hundred and forty consecutive DBE examinations were performed in 400 patients (250 males and 150 females, mean age 46.9 ± 16.3 years, range 14-86 years) between January 2007 and April 2012. Of these, 252 patients underwent the antegrade approach, and 188 patients underwent the retrograde approach. All the patients enrolled in our study were suspected of having small bowel diseases with a negative etiological diagnosis following other routine examinations, such as upper and lower gastrointestinal endoscopy and radiography tests. Data on tumors, such as clinical information, endoscopic findings and opera-tion results, were retrospectively collected. RESULTS: Small bowel tumors were diagnosed in 78 patients, of whom 67 were diagnosed using DBE, resulting in a diagnostic yield of 16.8% (67/400); the other 11 patients had negative DBE findings and were diagnosed through surgery or capsule endoscopy. Adenocarcinoma (29.5%, 23/78), gastrointestinal stromal tumor (24.4%, 19/78) and lymphoma (15.4%, 12/78) were the most common tumors. Among the 78 tumors, 60.3% (47/78) were located in the jejunum, and the overall number of malignant tumors was 74.4% (58/78). DBE examinations were frequently performed in patients with obscure gastrointestinal bleeding (47.4%) and abdominal pain (24.4%). The positive detection rate for DBE in the 78 patients with small bowel tumors was 85.9% (67/78), which was higher than that of a computed tomography scan (72.9%, 51/70). Based on the operation results, the accuracy rates of DBE for locating small bowel neoplasms, such as adenocarcinoma, gastrointestinal stromal tumor and lymphoma, were 94.4%, 100% and 100%, respectively. The positive biopsy rates for adenocarcinoma and lymphoma were 71.4% and 60%, respectively. CONCLUSION: DBE is a useful diagnostic tool with high clinical practice value and should be considered the gold standard for the investigation of small bowel tumors. | Wen-Guo Chen Guo-Dong Shan Hong Zhang Lin Li Min Yue Zun Xiang Ying Cheng Chen-Jiao Wu Ying Fang Li-Hua Chen | 2013 | World Journal of Gastroenterology2013，19，23： | 11 |
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