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| 1 | Burch colposuspension for stress urinary incontinence: a 14-year prospective follow-up显示文摘We aimed to evaluate the long-term effectiveness and safety of Burch colposuspension(BC) for stress urinary incontinence(SUI). In this prospective cohort study, 84 patients with SUI undergoing BC were enrolled from February 2004 to January 2010.Data on long-term subjective success and postoperative complications were collected at clinic visits and by telephone follow-up.During a mean follow-up period of 14.2 years, 68%(57/84) patients completed the follow-up. A total of 68.4% of patients(39/57) reported absence of SUI symptoms, 73.6%(42/57) were subjectively satisfied according to the Patient Global Impression of Improvement, and 68.4%(39/57) reported subjective success regarding urinary symptoms via the Urinary Distress Inventory Short Form. However, 28.1%(16/57) suffered at least one long-term postoperative complication and incident. Specifically, 1 in25(4.0%) sexually active patients reported dyspareunia, 3 patients(5.3%) had de novo overactive bladder, and 6 patients(10.5%)reported voiding dysfunction. Four patients(7.0%) reported new onset prolapse symptoms, and 3 patients(5.3%) underwent secondary urinary incontinence surgery. Our study indicated that Burch colposuspension is an effective procedure for SUI, and the cure effect was largely maintained for the 14-year follow-up period, with relatively low complication rates. BC should be considered a surgical option for SUI. | Yang Ye Yuan Wang Weijie Tian Zhibo Zhang Shuo Liang Xiaochen Song Jianbin Guo Qianqian Gao Honghui Shi Zhijing Sun Juan Chen Jinghe Lang Lan Zhu | 2022 | Science China(Life Sciences)2022，65，8： | 1 |
| 2 | Quantum Dot Nanobeads-Labelled Lateral Flow Immunoassay Strip for Rapid and Sensitive Detection of Salmonella Typhimurium Based on Strand Displacement Loop-Mediated Isothermal Amplification显示文摘Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food safety.In this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quantitative Salmonella Typhimurium(ST)detection.Quantum dot nanobeads(QBs)served as fluorescence reporters,providing good detection efficiency.The customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product capture.Detection was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)line.The linear detection range of the strip was 10^(2)–10^(8) colony forming units(CFU)·mL^(-1).The visual limit of detection was 10^(3) CFU·mL^(-1),indicating that the system was ten-fold more sensitive than AuNPs-labelled test strips.ST specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and SD-LAMP.We detected ST in foods with an acceptable recovery of 85%–110%.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis. | Yuting Shang Shuzhen Cai Qinghua Ye Qingping Wu Yanna Shao Xiaoying Qu Xinran Xiang Baoqing Zhou Yu Ding Moutong Chen Liang Xue Honghui Zhu Jumei Zhang | 2022 | Engineering2022，，12： | 0 |
| 3 | Spectrum-Efficient and Fair Resource Scheduling for MBMS in LTE Systems显示文摘An improved spectrum-efficient and fair resource scheduling algorithm for multimedia broadcast multicast service(MBMS)in long term evolution(LTE)systems is proposed in this paper.By jointly considering the channel conditions of all the users,the average packet loss rate,and the fairness of users in the MBMS group,the transmission data rate of the MBMS group is first selected according to the link adaptation and the average packet loss rate of users.Then,the resource blocks are allocated to MBMS groups according to the scheduling priority.Such a resource scheduling algorithm further balances the system throughput and user fairness.Theoretical analysis and simulation results show that the proposed algorithm can achieve a good tradeoff between system throughput and user fairness in comparison with traditional scheduling algorithms. | Xin Sun Honghui Ye Zhengyu Song | 2019 | Journal of Beijing Institute of Technology2019，28，4： | 0 |
| 4 | Caspase 3 siRNA decreases apoptosis in cultured neuronal cells显示文摘BACKGROUND:Lentiviral vectors,a type of retroviral vector,are able to infect cells at all phases of cell cycle.They are able to express exogenous target genes in vivo over long periods of time with limited immunological reaction. OBJECTIVE:To inhibit neuronal apoptosis by blocking the apoptotic cascade reaction,gene silencing of Caspase 3,and transfection of Caspase 3 short hairpin ribonucleic acid(shRNA) into Neuro 2a cells using a lentiviral vector. DESIGN,TIME AND SETTING:An observational,genetic engineering cellular biology experiment was performed in Guangzhou Red Cross Hospital and Guangzhou Institute of Traumatic Surgery between March 2007 and June 2008. MATERIALS:PLL3.7,PCMV-VSV-G,and PH'8.9△PR plasmids were provided by the CBR Institute for Biomedical Research,Harvard Medical School,USA.Staurosporine was purchased from Sigma, USA. METHODS:Caspase 3 siRNA was synthesized and cloned into the PLL3.7 plasmid.The Caspase 3 shRNA-PLL3.7 lentivirus was generated in 293T cells using a calcium phosphate transfection kit. After the lentivirus was transfected into Neuro 2a cells,apoptosis was induced in both the transfected and untransfected cells by staurosporine.Cell apoptosis was assessed by flow cytometry. MAIN OUTCOME MEASURES:Caspase 3 mRNA expression was measured by RT-PCR and Caspase protein expression was assessed by Western blot.Cellular apoptosis was determined by flow cytometry using Annevin V-PE/7aad-Cy7. RESULTS:The transfection rate of caspase 3 shRNA was>98%using the lentiviral vector.RT-PCR and Western blot results demonstrated that significantly reduced Caspase 3 mRNA and protein expression in the transfected Neuro 2a.The control group exhibited 38.7%Annexin V/7aad-positive cells,which suggested apoptotic anaphase,while only 5.0%cells in the Caspase 3 gene silencing group entered apoptotic anaphase. CONCLUSION:Caspase 3 shRNA inhibited Caspase 3 expression in Neuro 2a cells and decreased drug-induced apoptosis of Neuro 2a cells. | Chunting Ye Yaoxiong Huang Xiaohong Yang Honghui Chen | 2009 | Neural Regeneration Research2009，4，9： | 0 |
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