| 1 | The Structural Characterization and Antigenicity of the S Protein of SARS-CoV显示文摘The corona-like spikes or peplomers on the surface of the virion under electronicmicroscope are the most striking features of coronaviruses. The S (spike) proteinis the largest structural protein, with 1,255 amino acids, in the viral genome. Itsstructure can be divided into three regions: a long N-terminal region in the exte-rior, a characteristic transmembrane (TM) region, and a short C-terminus in theinterior of a virion. We detected fifteen substitutions of nucleotides by comparisonswith the seventeen published SARS-CoV genome sequences, eight (53.3%) of whichare non-synonymous mutations leading to amino acid alternations with predictedphysiochemical changes. The possible antigenic determinants of the S protein arepredicted, and the result is confirmed by ELISA (enzyme-linked immunosorbentassay) with synthesized peptides. Another profound finding is that three disulfidebonds are defined at the C-terminus with the N-terminus of the E (envelope) pro-tein, based on the typical sequence and positions, thus establishing the structuralconnection with these two important structural proteins, if confirmed. Phyloge-netic analysis reveals several conserved regions that might be potent drug targets. | JingxiangLi ChunqingLuo YajunDeng YujunHan LingTang JingWang JiaJi JiaYe FanboJiang ZhaoXu WeiTong WeiWei QingrunZhang ShengbinLi WeiLi HongyanLi YudongLi WeiDong JianWang ShengliBi HuanmingYaug | 2003 | Genomics, Proteomics & Bioinformatics2003，1，2： | 5 |
| 2 | VISCOUS FORCES BETWEEN TWO SPHERES COLLIDING THROUGH INTERSTITIAL POWER-LAW FLUID显示文摘Interaction between two spheres with an interstitial fluid is essential in Discrete Element modeling for simulating the behaviors of 'wet' particulate materials. In this paper the interaction between two spheres with an interstitial Power-law fluid was approximately resolved as normal and tangential interactive models respectively, for which the governing equations were simplified on the basis of Reynolds approximation. These equations were then solved an alytically together with the boundary conditions to obtain the pressure distributions for each individual model, and event ually solutions of the viscous squeeze force and the tangential viscous resistance were obtained, which provide a set of solutions for implementing into DEM code or other purposes. | YongXu HongyanLi WenbinHuang | 2005 | China Particuology2005，3，1： | 2 |
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