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| 1 | Hes1 Knockdown Exacerbates Ischemic Stroke Following tMCAO by Increasing ER Stress-Dependent Apoptosis via the PERK/ eIF2a/ATF4/CHOP Signaling Pathway显示文摘Apoptosis induced by endoplasmic reticulum(ER)stress plays a crucial role in mediating brain damage after ischemic stroke.Recently,Hes1(hairy and enhancer of split 1)has been implicated in the regulation of ER stress,but whether it plays a functional role after ischemic stroke and the underlying mechanism remain unclear.In this study,using a mouse model of ischemic stroke via transient middle cerebral artery occlusion(tMCAO),we found that Hes1 was induced following brain injury,and that siRNA-mediated knockdown of Hes1 increased the cerebral infarction and worsened the neurological outcome,suggesting that Hes1 knockdown exacerbates ischemic stroke.In addition,mechanistically,Hes1 knockdown promoted apoptosis and activated the PERK/eIF2a/ATF4/CHOP signaling pathway after tMCAO.These results suggest that Hes1 knockdown promotes ER stress-induced apoptosis.Furthermore,inhibition of PERK with the specific inhibitor GSK2606414 markedly attenuated the Hes1 knockdown-induced apoptosis and the increased cerebral infarction as well as the worsened neurological outcome following tMCAO,implying that the protection of Hes1 against ischemic stroke is associated with the amelioration of ER stress via modulating the PERK/eIF2a/ATF4/CHOP signaling pathway.Taken together,these results unveil the detrimental role of Hes1 knockdown after ischemic stroke and further relate it to the regulation of ER stress-induced apoptosis,thus highlighting the importance of targeting ER stress in the treatment of ischemic stroke. | Yueyong Li Yingjun Zhang Huangde Fu Huadong Huang Qifeng Lu Houji Qin Yingning Wu Huatuo Huang Guizhen Mao Zhongheng Wei Pinhu Liao | 2020 | Neuroscience Bulletin2020，36，2： | 12 |
| 2 | Group properties and invariant solutions of a sixth-order thin film equation in viscous fluid 显示文摘 | HUANGD J YANG Q M ZHOU S G | 2013 | J Math Phys2013，54，01： | 1 |
| 3 | Determination of tiamulin residue in pork and chicken by solid phase extraction and HPLC 显示文摘 | CHEN H C CHENG S H TSAI Y H HUANGD F | 2006 | J Food Drug Anal2006，14，1： | 1 |
| 4 | HighfrequencymagneticpropertiesofanamorphousFe78B13Si9ribbonimprovedbyACJouleheating显示文摘 | HuangD,LiJCM | 1991 | MaterSciEng1991，，133： | 1 |
| 5 | 红葡萄柚对冠状动脉粥样硬化患者血清甘油三酯水平的影响显示文摘 | Gorinstein S Caspi A Libman I LernerH T HuangD Leontowicz H 夏军辉 | 2006 | 中国动脉硬化杂志2006，14，6： | 1 |
| 6 | Determination of tiamulin residue in pork and chicken by solid phase extraction and HPLC显示文摘 | Chenh C Cheng S H Tsaiyh Huangd F | 2006 | J Food Drug Ana2006，14，1： | 1 |
| 7 | 鼻窦癌调强和三维适形治疗计划的比较[J]显示文摘 | HuangD XiaP AkazawaP 等 | | 国际放射肿瘤学生物物理杂志0，，： | 1 |
| 8 | Inhibition of MAPK kinase signaling pathways suppressed renal cell carcinoma growth and angiogenesis in vivo 显示文摘 | HuangD DingY Luo WM | 2008 | Cancer Res2008，68，1： | 1 |
| 9 | Optical coherence tomography显示文摘 | HuangD Swanson EA LinCP | 1991 | Science1991，254，5035： | 1 |
| 10 | DynamicchangesofendogenoushormonesinOsmanthusamericanusseedsduringcoldstratificatin[J]显示文摘 | HUANGD(黄丹) XUYX(许岳香) HUHB(胡海波) | 1971 | 林业科技开发1971，，： | 1 |
| 11 | Fabrication of star groove and rhombus grooves micro heat pipe 显示文摘 | Kangs Huangd | 2002 | Journal of Engineering2002，12，5： | 1 |
| 12 | Fusion of two malaria vaccine candidate antigens enhances product yield, immunogenicity, and antibody-mediated inhibition of parasite growth in vitro显示文摘A Plasmodium falciparum chimeric protein 2.9 (PfCP-2.9) was constructed consisting of the C-terminal regions of two leading malaria vaccine candidates, domain Ⅲ of apical membrane ag-1 (AMA-1) and 19-kDa C-terminal fragment of the merozoite surface protein 1 (MSP1). The PfCP-2.9 was produced by Pichia pastoris in secreted form with a yield of 2 600 mg/L and approximately 1 g/L of final product was obtained from a three-step purification process. Analysis of conformational properties of the chimeric protein showed that all six conformational mABS interacted with the recombinant protein were reduction-sensitive, indicating that fusion of the two cysteine-rich proteins retains critical conformational epitopes. PfCP-2.9 was found to be highly immunogenic in rabbits as well as in rhesus monkeys (Macaca mulatta). The chimeric protein induced both anti-MSP1-19 and anti-AMA-1(Ⅲ) ABS at levels 11- and 18-fold higher, respectively, than individual components did. Anti-PfCP-2.9 sera from both rabbits and rhesus monkeys almost completely inhibited in vitro growth of the P.falciparum FCC1/HN and 3D7 lines when tested at a 6.7-fold dilution. It was shown that the inhibition is dependent on the presence of ABS to the chimeric protein and their disulfide bond-dependent conformations. Moreover, the activity was mediated by a combination of growth-inhibitory ABS generated by the individual MSP1-19 and AMA-1(Ⅲ) of PfCP-2.9. The combination of the extremely high yield of the protein and enhancement of its immune response provides a basis to develop an effective and affordable malaria vaccine. | PanW HuangD ZhangQ QuL ZhangD ZhangX XueX QianF | 2005 | 第二军医大学学报2005，26，4： | 1 |
| 13 | Thegas7proteinpotentiatesNGF-mediateddifferentiationofPC12cells显示文摘 | LortieK HuangD ChakravarthyB etal | 2005 | BrainRes2005，1036，12： | 1 |
| 14 | Laparoscopic versus open appen- dectomy a prospective randomized comparison 显示文摘 | Wei HB HuangD Zheng ZH | 2010 | Surg Endosc2010，24，2： | 1 |
| 15 | Opticalcoherencetomography显示文摘 | HUANGD SWANSONEA LINCP eta1 | 1991 | Science1991，254，5035： | 1 |
| 16 | Reproduction Obstacles for the Female Green Neon Shrimp(Neocaridina denticulata)After Exposure to Chlordane and Lindane显示文摘 | HUANGD J CHEN H C WU JP | 2006 | Chemosphere2006，64，1： | 1 |
| 17 | Parentallyimprintedallele(PIA)typinginthedifferentiallymethylatedregionupstreamofthehumanH19gene显示文摘 | HuangD LinX ChenH etd | 2008 | ForensicSciIntGenet2008，2，4： | 1 |
| 18 | Pro- tein tyrosine dephosphorylation during copper-induced cell death in rice roots显示文摘 | HUNGWC HUANGD D CHIENPS | 2007 | Chemosphere2007，69，： | 1 |
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