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| 1 | Association between low molecular polypeptide 7 single nucleotide polymorphism and response to therapy in hepatitis C virus infection显示文摘AIM: To investigate the relationship between low molecular polypeptide-7 (LMP-7) gene polymorphism and response to interferon (IFN) therapy in chronic hepatitis C virus (HCV) patients. METHODS: LMP-7 polymorphism at codon 49 with nucleotide substitution from A to C was amplified in 104 chronic HCV patients of genotype 4. The amplicons were digested with restriction endonuclease Bsm I and the produced restriction fragment length polymorphism was analyzed. Patients received IFN + regional blood volume therapy for 48 wk and the frequency of thissingle nucleotide polymorphism (SNP) was statistically correlated with treatment response. The exclusion criteria for these patients were stated by the national health program for treating viral hepatitis. Main exclusion criteria included co-infection with hepatitis B virus or schistosomiasis, thyroid dysfunction, uncontrolled diabetes mellitus, history of long term drug or alcohol intake and autoimmune hepatitis. Multivariate analyses were done to correlate LMP-7 SNP plus several factors such as age, gender, weight, serum alpha-fetoprotein (AFP) and alanine aminotransferase levels, liver activity, fibrosis score and viral load with response to therapy. RESULTS: The data presented in this study clearly demonstrated statistically significant differences between sustained virological response (SVR) (defined as the absence of HCV RNA levels in the patient's sera at least 6 mo after discontinuation of treatment) and non-response (NR) (where HCV RNA levels in the patient's sera never become undetectable for 6 mo during or after treatment). Variables were described as odds ratio with 95%CI. The data were considered significant if P values were ≤ 0.05; highly significant if P < 0.01 and very highly significant if P < 0.001. Current data showed that 91.7% of patients carrying LMP-7 C/C allele were associated with SVR, while the other two genotypes C/A and A/A were associated with NR patients, 83.3% and 64.3% respectively, showing that genotype CC was strongly associated with response to interferon (95%CI: 12.0719-134.6572, P = 0.0001). The majority of parameters recorded in SVR and NR patients included higher values of mean age (P = 0.004), alanine aminotransferase (P = 0.001), AFP (P = 0.001), body weight (P = 0.025), viral load (P = 0.025), higher fibrosis and histological activity index indices among NR vs SVR patients. Also, the multivariate statistical analysis of the different factors of fibro-sis score, liver activity grade, genotypes and alleles of LMP-7 gene polymorphism in responders and NRs of HCV patients in this study showed that HCV patients with A allele had a very highly significant association with the NRs, high fibrosis and higher liver activity, while the C allele had a very highly significant association with the responders, low fibrosis and lower liver activity (95%CI: 3.5800-13.2519, P = 0.0001).CONCLUSION: LMP-7 SNP is a candidate gene that should be considered when designing a mathematical model for predicting response to therapy and disease progression in HCV patients. | Moataza H Omran Basma E Fotouh Samar S Youssef Noha E Ibrahim Wael Nabil EL-Sayed M Mahdy Wafaa G Shosha Mostafa K El-Awady | 2013 | World Journal of Hepatology2013，5，3： | 4 |
| 2 | Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma显示文摘AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,Egypt.Fifteen wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal controls.Laboratory investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn't express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and p27.Cyclin D1 expressed nuclear staining that was sequentially increased from CHC to LC(P<0.01) to HCC(P<0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 immuno-reactivity.The expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied cases.Correlation analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P<0.001) ,between Cyclin D1 and Cyclin E in HCCs(P<0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P<0.001) and between p21 and Rb/p105(P<0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC plays a vital role in the development and differentiation of HCC;while,Cyclin E may be a useful marker for monitoring tumor behavior.p21 and p27 can be used as predictive markers for HCC.Furthermore,higher expression of Rb/p105 as well as inverse relation with p21 and histologic grades suggests its important role in hepatic carcinogenesis. | Azza E El Bassiouny Mona M Nosseir Mona K Zoheiry Noha A Ameen Ahmed M Abdel-Hadi Ibrahim M Ibrahim Suher Zada Abdel-Hakeem Saad El-Deen Nora E El-Bassiouni | 2010 | World Journal of Hepatology2010，2，1： | 3 |
| 3 | Effect of dietary protein level and strain on growth performance of heat stressed broiler chicks显示文摘 | Rashid H O S Huwaida E E M Ibrahim Y A | 2012 | International Journal of Poultry Science2012，11，10： | 1 |
| 4 | Rapid determination of chloramphenicol and its glucuronide in food products by liquid chromatography-electrospray negative ionization tandem mass spectrometry显示文摘 | Bogusz M J Hassan H A1-Enazi E Ibrahim Z A1-Tufail M | 2004 | Journal of Chromatography B2004，807，： | 1 |
| 5 | Traveling- wave -based fault- location scheme for multiend- aged underground cable system显示文摘 | GILANY M G IBRAHIM D K SAYED E | 2007 | IEEE Transactions on Power Delivery2007，22，1： | 1 |
| 6 | Traveling-wave-based fault-location scheme for multiend-aged underground cable system 显示文摘 | GILANY M IBRAHIM D K ELDIN E T | 2007 | IEEE Transactions on Power Delivery2007，22，1： | 1 |
| 7 | Mitral stenosis after mitral valve repair for non-rheumatic mitral regurgitation显示文摘 | Ibrahim M F David T E | | 0，，： | 1 |
| 8 | Three- photon-induced luminescence of gold nanoparticles embedded in and located on the surface of glassy nanolayers 显示文摘 | EICHELBAUM M SCHMIDT B E IBRAHIM H | 2007 | Nanotechnol- ogy2007，18，35： | 1 |
| 9 | Effect of lead acetate toxicity on experimental male albino rat显示文摘Objective:To evaluate the effect of different doses of lead acetate(1/20,1/40 and 1/60 of LD_(50))on body weight gain,blood picture,plasma prolein profile and the function of liver,kidney and thyroid gland.Methods:Male albino rats were divided into four groups,the first group represented the health control animals,while the second,third and fourth groups were ingested orally with sub lethal doses of load acetate(1/20,1/40 and 1/60)of the oral LD_(50),respectively.One dose was ingested every two days during the experimental period(14 weeks)including the adaptation time.Blood was collected and used for all analysis.Results:The results showed that,the ingestion of Pb^(2+)induced significant stimulation in glutamic-pyruvic transaminase(ALT)and glutamic-oxalacetic transaminease(AST)activity.Also,total soluble protein and albumin contents of plasma were significantly decreased,while the content of globulin was changed by the Pb^(2+)treatments.The cholinesteiase activity was inhibited,but the activities of alkaline and acid phosphates and lactate dehydrogenase were stimulated,while plasma glucose level was elevated as a result of lead acetate intoxication.In case of blood picture,Pb^(2+)ingestion reduced the contents of hemoglobin and RBCs count of intoxicated rat's blood and the plasma levels of T3,T4 and blood WBCs count were decreased.Conclusions:It can be concluded that lead acetate has harmful effect on experimental male albino rats.Therefore,the present work advises people to prevent exposure to the lead compound to avoid injurious hazard risk. | Nabil M Ibrahim Esam A Eweis Hossam S El-Beltagi Yasmin E Abdel-Mobdy | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，1： | 1 |
| 10 | Monitoring of fatigue cracks using permanently-mounted conformable eddy-current sensors显示文摘 | Ibrahim M E Ditchburn R J | | 0，，33： | 1 |
| 11 | Spectrophotometric methods for the determination ofcefprozil in bulk and dosage form显示文摘 | Gadkariem E A Mutasim M M Ibrahim K E | 2009 | Int J Biomed Sci2009，5，3： | 1 |
| 12 | Effects of preparation conditions on the monodispersity of albumin microspheres 显示文摘 | Ibrahim E - S Safwat S | 1998 | Journal of Microencapsulation1998，15，5： | 1 |
| 13 | The hypocholesterolaemic effect of milk yoghurt and Soy-yoghurt containing bifidobacteria in rats fed on a Cholesterol- enriched diet 显示文摘 | Ibrahim A hbd El-Gawad E M El-Sayed S A Hafez | 2005 | International Dairy Journal2005，15，： | 1 |
| 14 | Forced Convection and Entro- py Generation from Elliptic Tubes with Longitudinal Fins显示文摘 | Ibrahim E Moawed M | 2009 | Energy Conversion and Management2009，50，8： | 1 |
| 15 | Early vegetative growth of cotton as influenced by leaf type显示文摘 | Ibrahim M E | 1994 | Crop Sci1994，21，： | 1 |
| 16 | Modified Egyptian talc as internal and surface treatments for papermaking显示文摘 | Ibrahim M M Mobarak F EI-Din E I S | 2009 | Tappi Journal2009，，4： | 1 |
| 17 | Thin films of chromium electrodeposition from a trivalent chromium electrolyte显示文摘 | Abd E R S S Ibrahim M A M Dankeria M M | 2002 | Transactions of the Institute of Metal Finishing2002，80，1： | 1 |
| 18 | Gene-expression profiling of experimental autommune encephalomyelitis显示文摘 | Mix E Pahnke J Ibrahim M | 2002 | Neurochem Res2002，27，10： | 1 |
| 19 | Neural networks for job shop schedu- ling problems显示文摘 | Ibrahim S EI-Baz M A Esh E | 2009 | Journal of Engineering and Applied Science2009，56，2： | 1 |
| 20 | Enhanced Intestinal Expression of Heat Shock Protein 70 in Patients with Inflammatory Bowel Diseases显示文摘 | Ludwig D Stahl M Ibrahim M E T | | 0，，44： | 1 |
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