|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Arabidopsis BAG1 Functions as a Cofactor in Hsc70-Mediated Proteasomal Degradation of Unimported Plastid Proteins显示文摘 | Dong Wook Lee Su Jin Kim Young Jun Oh Bongsoo Choi Juhun Lee Inhwan Hwang | 2016 | Molecular Plant2016，9，10： | 8 |
| 2 | The AP-3 adaptor complex is required for vacuolar function in Arabidopsis显示文摘Subcellular trafficking 在真核细胞的房间为大量的功能被要求。它包含货物排序,在多重层次的泡形成, trafficking 和熔化过程的规定。适配器蛋白质(AP ) 建筑群是货物在酵母和哺乳动物排序进泡的关键管理者,但是他们在植物的存在和功能没被表明。这里,我们报导影响蛋白质的 trafficking 的鉴定 4 (pat4 ) 在通常认为的 δ 的变异的有缺陷者;AP-3 建筑群的子单元。pat4 和 pat2,从缺乏功能的通常认为的 AP-3 β 的一样的 GFP 基于成像的向前基因的屏幕孤立的异种;,以及主导的否定 AP-3 μ难辨别的显型由大部分正常的形态学和开发描绘了的转基因的线显示,而是在异常 vacuolar 结构的膜蛋白质的强壮的细胞内部的累积。所有异种在形态学和功能是有缺点的 lysin 并且蛋白质存储液泡(PSV ) 但是储备蛋白质排序到 PSV 的表演正常。Immunoprecipitation 实验和基因研究揭示了通常认为的 AP-3 β 的紧密的功能、物理的协会;并且 AP-3 δ子单元。而且,两蛋白质仔细与通常认为的 AP-3 μ 被连接;并且 σ子单元和 clathrin 和 dynamin 机械的几个部件。一起拿,这些结果表明那 AP 建筑群,类似于那些在另外的优核质,在植物,和那 AP-3 存在在植物房间在生物的续生说的规定和液泡的功能起一个特定的作用。 | Marta Zwiewka Elena Feraru Barbara Moller Inhwan Hwang Mugurel I Feraru Jargen Kleine-Vehn Dolf Weijers Jifi Friml | 2011 | Cell Research2011，21，12： | 3 |
| 3 | Molecular Mechanism of the Specificity of Protein Import into Chloroplasts and Mitochondria in Plant Cells显示文摘Plants possess both types of endosymbiotic organelles, chloroplasts and mitochondria. Transit peptides and presequences function as signal sequences for specific import into chloroplasts and mitochondria, respectively. However, how these highly similar signal sequences confer the protein import specificity remains elusive. Here, we show that mitochondrial- or chloroplast-specific import involves two distinct steps, specificity determination and translocation across envelopes, which are mediated by the N-terminal regions and functionally interchangeable C-terminal regions, respectively, of transit peptides and presequences. A domain harboring multiple-arginine and hydrophobic sequence motifs in the N-terminal regions of presequences was identified as the mitochondrial specificity factor. The presence of this domain and the absence of arginine residues in the N-terminal regions of otherwise common targeting signals confers specificity of protein import into mitochondria and chloroplasts, respectively. AtToc159, a chloroplast import receptor, also contributes to determining chloroplast import specificity. We propose that common ancestral sequences were functionalized into mitochondrial- and chloroplast-specific signal sequences by the presence and absence, respectively, of multiple-arginine and hydrophobic sequence motifs in the N-terminal region. | Dong Wook Lee Sumin Lee Junho Lee Seungjin Woo Md.Abdur Razzak Alessandro Vitale Inhwan Hwang | 2019 | Molecular Plant2019，12，7： | 2 |
| 4 | Dynamic spatial reorganization of BSK1 complexes in the plasma membrane underpins signal-specific activation for growth and immunity显示文摘Growth and immunity are opposing processes that compete for cellular resources,and proper resource allocation is crucial for plant survival.BSK1 plays a key role in the regulation of both growth and immunity by associating with BRI1 and FLS2,respectively.However,it remains unclear how two antagonistic signals co-opt BSK1 to induce signal-specific activation.Here we show that the dynamic spatial reorganiization of BSK1 within the plasma membrane underlies the mechanism of signal-specific activation for growth or immunity.Resting BSK1 localizes to membrane rafts as complexes.Unlike BSK1-associated FLS2 and BRI1,flg22 or exogenous brassinosteroid(BR)treatment did not decrease BSK1 levels at the plasma membrane(PM)but rather induced BSK1 multimerization and dissociation from FLS2/BSK1 or BRI1/BSK1,respectively.Moreover,flg22-activated BSK1 translocated from membrane rafts to non-membrane-raft regions,whereas BR-activated BSK1 remained in membrane rafts.When applied together with flg22,BR suppressed various flg22-induced BSK1 activities such as BSK1 dissociation from FLS2/BSK1,BSK1 interaction with MAPKKK5,and BSK translocation together with MAPKKK5.Taken together,this study provides a unique insight into how the precise control of BSK1 spatiotemporal organization regulates the signaling specificity to balance plant growth and immunity. | Bodan Su Xi Zhang Li Li Sammar Abbas Meng Yu Yaning Cui Frantisek Baluska Inhwan Hwang Xiaoyi Shan Jinxing Lin | 2021 | Molecular Plant2021，14，4： | 2 |
| 5 | Adaptor Protein Complex 2–Mediated Endocytosis Is Crucial for Male Reproductive Organ Development in Arabidopsis[W]显示文摘 | Soo Youn Kim Zheng-Yi Xu Kyungyoung Song Dae Heon Kim Hyangju Kang Ilka Reichardt Eun Ju Sohn Ji?í Friml Gerd Juergens Inhwan Hwang | 2013 | The Plant Cell2013，，8： | 1 |
| 6 | Clathrin-Mediated Constitutive Endocytosis of PIN Auxin Efflux Carriers in Arabidopsis显示文摘 | Pankaj Dhonukshe Fernando Aniento Inhwan Hwang David G. Robinson Jozef Mravec York-Dieter Stierhof Ji?í Friml | 2007 | Current Biology2007，，6： | 1 |
| 7 | Isolation and characterization of two β-tubulin cDNA clones from rice显示文摘 | Mee Sun Kang Young Ju Choi Min Chul Kim Chae Oh Lim Inhwan Hwang Moo Je Cho | 1994 | Plant Molecular Biology1994，，6： | 1 |
| 8 | Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when expressed in Vicia faba guard cells显示文摘 | Joohyun Lee Shim Donghwan Song Won-yong Hwang Inhwan Lee Youngsook | 2004 | Plant Molecular Biology2004，，6： | 1 |
| 9 | Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when expressed in Vicia faba guard cells显示文摘 | Joohyun Lee Shim Donghwan Song Won-yong Hwang Inhwan Lee Youngsook | 2004 | Plant Molecular Biology2004，，6： | 1 |
| 10 | Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds显示文摘 | Chae Oh Lim Soo In Lee Woo Sik Chung Sung Han Park Inhwan Hwang Moo Je Cho | 1996 | Plant Molecular Biology1996，，2： | 1 |
| 11 | Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when expressed in Vicia faba guard cells显示文摘 | Joohyun Lee Shim Donghwan Song Won-yong Hwang Inhwan Lee Youngsook | 2004 | Plant Molecular Biology2004，，6： | 1 |
| 12 | Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants显示文摘根围使发酸为铁(Fe ) 是必要的举起进植物根。在根围使发酸的血浆膜(下午) H +-ATPases 戏钥匙角色。然而,下午 H +-ATPase 活动怎么被调整在 Fe 缺乏的条件下面提高根 Fe 举起,充分没被理解。这里,我们现在的证据那细胞色素 b5 reductase (CBR1 ) 1 增加不饱和的丰满的酸的层次,它 stimulatePM H +-ATPase 活动并且这样 leadto 根围使发酸。不饱和的丰满的酸(18:2 和 18:3 ) 高级的有的 CBR1-overexpressing (CBR1 公牛) Arabidopsis thaliana 植物,更高级的下午 H +-ATPase 活动,和比野类型的植物的更低的根围 pH。由对比, cbr1loss-of-function 异种植物显示出不饱和的丰满的酸和更低的下午 H +-ATPase 活动的底层但是更高的根围 pH。在 cbr1 的减少的下午 H +-ATPase 活动能是由不饱和的丰满的酸的增加的恢复 invitro。CBR1,丰满的酸 desaturase2 (FAD2 ) ,和丰满的酸 desaturase3 (FAD3 ) 的抄本层次在 Fe 缺乏的条件下面被增加。我们建议 CBR1 在增加不饱和的丰满的酸的层次有一个关键角色,它 activatethe 下午 H +-ATPase 并且这样 reducerhizosphere pH。这反应串联最终支持根 Fe 举起。 | Young Jun Oh Hanul Kim Sung Hee Seo Bae Geun Hwang Yoon Seok Chang Junho Lee Dong Wook Lee Eun Ju Sohn Sang Joon Lee Youngsook Lee Inhwan Hwang | 2016 | Molecular Plant2016，9，4： | 1 |
| 13 | The A/ENTH Domain-Containing Protein AtECA4 Is an Adaptor Protein Involved in Cargo Recycling from the trans-Golgi Network/Early Endosome to the Plasma Membrane显示文摘Endocytosis 和随后的 trafficking 小径为调整血浆的活动是关键的膜局部性的蛋白质。取决于细胞、生理的条件,使内在化的货物被排序在(并且搬运了从) trans-Golgi network/early 内涵体(TGN/EE ) 到为降级的液泡或再循环回到血浆膜。这怎么发生在分子的水平,留下大部分逃犯。这里,我们提供 ENTH 包含域的蛋白质 AtECA4 在在 Arabidopsis thaliana 从 TGN/EE 再循环货物到血浆膜起一个关键作用的证据。对 TGN/EE 和血浆膜首先局部性的 AtECA4:sGFP (在底层) 。在 NaCl 或甘露糖醇处理之上, AtECA4:sGFP 在一个早时间点在 TGN/EE 积累了,但是在以后的时间点被免除 TGN/EE 到 cytosol。ateca4 异种比野类型显示出更高的抵抗到渗透的应力和对外长的 abscisic 酸(骆驼毛的织物) 敏感的更多,以及增加了骆驼毛的织物可诱导的基因 RD29A 和 RD29B 的表示。一致地, ABCG25,血浆膜局部性的骆驼毛的织物出口商,在 ateca4 在 prevacuolar 分隔空间积累了,在再循环到血浆膜显示一个缺点。然而,在货物再循环的 AtECA4 的角色不对 ABCG25 特定,当它也在再循环 BRI1 工作。这些结果建议 AtECA4 在从 TGN/EE 再循环 endocytosed 货物到血浆膜起一个关键作用。 | Hong Hanh Nguyen Myoung Hui Lee Kyungyoung Song Gyeongik Ahn Jihyeong Lee Inhwan Hwang | 2018 | Molecular Plant2018，11，4： | 1 |
| 14 | Interactions between Transmembrane Helices within Monomers of the Aquaporin AtPIP2;1 Play a Crucial Role in Tetramer Formation显示文摘Aquaporin (AQP ) 是在原核生物、真核细胞的房间的各种各样的 subcellular 膜发现的水隧道蛋白质。AQP 的生理的功能在许多有机体被阐明了。然而,理解他们的生物的续生说留下逃犯,特别地考虑他们怎么集合进 tetramers。这里,我们调查了涉及 Arabidopsis 血浆膜 AQP AtPIP2 的 tetramer 形成的氨基酸残余; 1 使用的广泛的氨基酸替换 mutagenesis。变异的蛋白质 V41A/E44A, F51A/L52A, F87A/I91A, F92A/I93A, V95A/Y96A,和 H216A/L217A,在 transmembrane (TM ) 怀有丙氨酸替换 AtPIP2 的 helices; 1 polymerized 进有比四大的子单元的一个可变数字的多重 oligomeric 建筑群。而且,这些变异的蛋白质失败了到交通到血浆膜,而不是在 endoplasmic 蜂窝胃积累(嗯) 。基于结构的建模表明这些残余大部分在单体以内涉及在 TM helices 之间的相互作用。这些结果建议在以内并且在单体之间发生的 inter-TM 相互作用在 AtPIP2 在 tetramer 形成起关键作用; 1 建筑群。而且, AtPIP2 的集会; 1 tetramers 为他们从 ER 的 trafficking 是批评的到血浆膜,以及水渗透。 | Yun-Joo Yoo Hyun Kyung Lee Wonhee Han Dae Heon Kim Myoung Hui Lee Jouhyun Jeon Dong Wook Lee Junho Lee Yongjik Lee Juhun Lee Jin Seok Kim Yunje Cho Jin-Kwan Han Inhwan Hwang | 2016 | Molecular Plant2016，9，7： | 0 |
| 15 | Liquid-Liquid Phase Transition as a New Means of Protein Targeting in Chloroplasts显示文摘The chloroplast is a unique organelle in that it possesses an intra-organellar membrane-enclosed structure called thylakoid,which is central to photosynthesis.Thus,the timely expression and correct localization of thylakoid proteins are of utmost importance to plant growth and development(Lee et al.,2017;New et al.,2018).Biogenesis of thylakoid proteins is highly complex;they are encoded by two different genomes,nuclear and chloroplast genomes,and translated in the cytosol and chloroplasts,respectively.Nuclear-encoded thylakoid proteins are first imported into the stroma of chloroplasts before they are targeted to thylakoids.In the stroma,multiple sorting mechanisms exist to ensure import or insertion of thylakoid luminal or membrane proteins,respectively(Lee et al.,2017).Approximately 50%of thylakoid luminal proteins are imported through the cpTAT(chloroplast twin-arginine translocation)pathway,which is also present in bacteria and plant mitochondria(Cline,2015;Schafer et al.f 2020). | Dong Wook Lee Inhwan Hwang | 2020 | Molecular Plant2020，13，5： | 0 |
| 16 | Both metaxin and Tom20 together with two mitochondria-specific motifs support mitochondrial targeting of dual-targeting AtSufE1显示文摘Plant cells have two endosymbiotic organelles,chloroplasts,and mitochondria.These organelles perform specific functions that depend on organelle-specific proteins.The majority of chloroplast and mitochondrial proteins are specifically imported by the transit peptide and presequence,respectively.However,a significant number of proteins are also dually targeted to these two organelles.Currently,it is not fully understood how proteins are dually targeted to both chloroplasts and mitochondria.In this study,the mechanism underlying mitochondrial targeting of dual targeting At Suf E1 in Arabidopsis was elucidated.The N-terminal fragment containing 80 residues of AtSufE1(AtSufE1N80)was sufficient to confer dual targeting of reporter protein,At SufE1N80:GFP,in protoplasts.Two sequence motifs,two arginine residues at 15and 21positions,and amino acid(aa)sequence motif AKTLLLRPLK from the 31to 40aa position,were responsible for targeting to mitochondria a portion of reporter proteins amid the chloroplast targeting.The sequence motif PSEVPFRRT from the 41 stto 50 thaa position constitutes a common motif for targeting to both chloroplasts and mitochondria.For mitochondrial import of At Suf E1:N80,Metaxin played a critical role.In addition,Bi FC and protein pull-down experiments showed that At Suf E1N80 specifically interacts with import receptors,Metaxin and Tom20.The interaction of At Suf E1N80 with Metaxin was required for the interaction with Tom20.Based on these results,we propose that mitochondrial targeting of dual-targeting At Suf E1 is mediated by both mitochondria-specific and common sequence motifs in the signal sequence through the interaction with import receptors,Metaxin and Tom20,in a successive manner. | Seungjin Woo Byeongho Moon Inhwan Hwang | 2022 | Journal of Integrative Plant Biology2022，64，8： | 0 |
| 17 | Understanding protein translocation across chloroplast membranes:Translocons and motor proteins显示文摘Subcellular organelles in eukaryotes are surrounded by lipid membranes.In an endomembrane system,vesicle trafficking is the primary mechanism for the delivery of organellar proteins to specific organelles.However,organellar proteins for chloroplasts,mitochondria,the nucleus,and peroxisomes that are translated in the cytosol are directly imported into their target organelles.Chloroplasts are a plant-specific organelle with outer and inner envelope membranes,a dual-membrane structure that is similar to mitochondria.Interior chloroplast proteins translated by cytosolic ribosomes are thus translocated through TOC and TIC complexes(translocons in the outer and inner envelope of chloroplasts,respectively),with stromal ATPase motor proteins playing a critical role in pulling pre-proteins through these import channels.Over the last three decades,the identity and function of TOC/TIC components and stromal motor proteins have been actively investigated,which has shed light on the action mechanisms at a molecular level.However,there remains some disagreement over the exact composition of TIC complexes and genuine stromal motor proteins.In this review,we discuss recent findings on the mechanisms by which proteins are translocated through TOC/TIC complexes and discuss future prospects for this field of research. | Da Been Kim Changhee Na Inhwan Hwang Dong Wook Lee | 2023 | Journal of Integrative Plant Biology2023，65，2： | 0 |
| 18 | Translation rate underpins specific targeting of N-terminal transmembrane proteins to mitochondria显示文摘Protein biogenesis is a complex process,and complexity is greatly increased in eukaryotic cells through specific targeting of proteins to different organelles.To direct targeting,organellar proteins carry an organelle-specific targeting signal for recognition by organelle-specific import machinery.However,the situation is confusing for transmembrane domain(TMD)-containing signalanchored(SA)proteins of various organelles because TMDs function as an endoplasmic reticulum(ER)targeting signal.Although ER targeting of SA proteins is well understood,how they are targeted to mitochondria and chloroplasts remains elusive.Here,we investigated how the targeting specificity of SA proteins is determined for specific targeting to mitochondria and chloroplasts.Mitochondrial targeting requires multiple motifs around and within TMDs:a basic residue and an arginine-rich region flanking the N-and C-termini of TMDs,respectively,and an aromatic residue in the C-terminal side of the TMD that specify mitochondrial targeting in an additive manner.These motifs play a role in slowing down the elongation speed during translation,thereby ensuring mitochondrial targeting in a cotranslational manner.By contrast,the absence of any of these motifs individually or together causes at varying degrees chloroplast targeting that occurs in a post-translational manner. | Junho Lee Byeongho Moon Dong Wook Lee Inhwan Hwang | 2023 | Journal of Integrative Plant Biology2023，65，6： | 0 |
| 19 | Plant-based,adjuvant-free,potent multivalent vaccines for avian influenza virus via Lactococcus surface display显示文摘Influenza epidemics frequently and unpredictably break out all over the world,and seriously affect the breeding industry and human activity.Inactivated and live attenuated viruses have been used as protective vaccines but exhibit high risks for biosafety.Subunit vaccines enjoy high biosafety and specificity but have a few weak points compared to inactivated virus or live attenuated virus vaccines,especially in low immunogenicity.In this study,we developed a new subunit vaccine platform for a potent,adjuvant-free,and multivalent vaccination.The ectodomains of hemagglutinins(HAs)of influenza viruses were expressed in plants as trimers(tHAs)to mimic their native forms.tHAs in plant extracts were directly used without purification for binding to inactivated Lactococcus(iLact)to produce iLact-tHAs,an antigen-carrying bacteria-like particle(BLP).tHAs BLP showed strong immune responses in mice and chickens without adjuvants.Moreover,simultaneous injection of two different antigens by two different formulas,t^(HAH5N6+H9N2) BLP or a combination of t^(HAH5N6) BLP and t^(HAH9N2) BLP,led to strong immune responses to both antigens.Based on these results,we propose combinations of plant-based antigen production and BLP-based delivery as a highly potent and cost-effective platform for multivalent vaccination for subunit vaccines. | Shi-Jian Song Gyeong-Im Shin Jinyong Noh Jiho Lee Deok-Hwan Kim Gyeongryul Ryu Gyeongik Ahn Hyungmin Jeon Hai-Ping Diao Youngmin Park Min Gab Kim Woe-Yeon Kim Young-Jin Kim Eun-Ju Sohn Chang Seon Song Inhwan Hwang | 2021 | Journal of Integrative Plant Biology2021，63，8： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员