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| 1 | Effect of glycyrrhizic acid and 18β-glycyrrhetinic acid on the differentiation of human umbilical cord-mesenchymal stem cells into hepatocytes显示文摘BACKGROUND End-stage liver disease is a global health complication with high prevalence and limited treatment options.Cell-based therapies using mesenchymal stem cells(MSCs)emerged as an alternative approach to support hepatic regeneration.In vitro preconditioning strategies have been employed to strengthen the regenerative and differentiation potential of MSCs towards hepatic lineage.Chemical compounds of the triterpene class;glycyrrhizic acid(GA)and 18β-glycyrrhetinic acid(GT)possess diverse therapeutic properties including hepatoprotection and anti-fibrosis characteristics.They are capable of modulating several signaling pathways that are crucial in hepatic regeneration.Preconditioning with hepato-protective triterpenes may stimulate MSC fate transition towards hepatocytes.AIM To explore the effect of GA and GT on hepatic differentiation of human umbilical cord-MSCs(hUC-MSCs).METHODS hUC-MSCs were isolated and characterized phenotypically by flow cytometry and immunocytochemistry for the expression of MSC-associated surface molecules.Isolated cells were treated with GA,GT,and their combination for 24 h and then analyzed at three time points;day 7,14,and 21.qRT-PCR was performed for the expression of hepatic genes.Expression of hepatic proteins was analyzed by immunocytochemistry at day 21.Periodic acid Schiff staining was performed to determine the functional ability of treated cells.RESULTS The fusiform-shaped morphology of MSCs in the treatment groups in comparison with the untreated control,eventually progressed towards the polygonal morphology of hepatocytes with the passage of time.The temporal transcriptional profile of preconditioned MSCs displayed significant expression of hepatic genes with increasing time of differentiation.Preconditioned cells showed positive expression of hepatocyte-specific proteins.The results were further corroborated by positive periodic acid Schiff staining,indicating the presence of glycogen in their cytoplasm.Moreover,bi-nucleated cells,which is the typical feature of hepatocytes,were also seen in the preconditioned cells.CONCLUSION Preconditioning with glycyrrhizic acid,18β-glycyrrhetinic acid and their combination,successfully differentiates hUC-MSCs into hepatic-like cells.These MSCs may serve as a better therapeutic option for degenerative liver diseases in future. | Abiha Fatima Tuba Shakil Malick Irfan Khan Aisha Ishaque Asmat Salim | 2021 | World Journal of Stem Cells2021，13，10： | 3 |
| 2 | A comprehensive MATLAB Simulink PV system simulator with partial shading capability based on two-diode model显示文摘 | Kashif Ishaque Zainal Salam Syafaruddin | 2011 | Solar Energy2011，，9： | 2 |
| 3 | A comprehensive MATLAB SimulinkPV system simulator with partial shading capability based ontwo-diode model 显示文摘 | Ishaque K Salam Z | 2011 | Solar Energy2011，85，9： | 1 |
| 4 | A direct control based maxi- mum power point tracking method for photovoltaic system under partial shading conditions using particle swarm optimization algo- rithm显示文摘 | Ishaque K Salam Z Shamsudin A | 2012 | Applied Energy2012，99，： | 1 |
| 5 | Knee function and prevalence of osteoarthritis after isolated anterior cruciate ligament reconstruction using bone-patellar tendon-bone graft:long-term follow-up显示文摘 | Struewer J Frangen TM Ishaque B | 2012 | Int Orthop2012，36，1： | 1 |
| 6 | Why do maize hybrds re- spond differently to variations in plant density显示文摘 | Bukhsh M A Ahmad R Ishaque M | 2011 | Crop & Environ- ment2011，2，1： | 1 |
| 7 | Atorzine potentiation of arsonic trioxide-induced cytotoxicity and gene expression in human liver carcinoma cells (H epG2) 显示文摘 | Tchounwou PB Wilson BA Ishaque AB | 2001 | Mol Cell Biochem2001，222，： | 1 |
| 8 | Atrazine potentiation of arsenic trioxide-induced cytotoxicity and gene expression in human liver carcinoma cells ( HepG2 ) 显示文摘 | Tchounwou PB Wilson BA Ishaque AB | 2001 | Mol Cell Bioehem2001，222，112： | 1 |
| 9 | Polymer,metal,and hybrid nano-and mesotubes by coating degradable polyser template fibers (TUFT Process)显示文摘 | Bognitzki M Hou H Ishaque M | 2000 | Adv Mater2000，12，9： | 1 |
| 10 | Rhodiola rosea for physical and mental fatigue:a systematic review显示文摘 | Ishaque S Shamseer L Bukutu C | | BMC Complementary and Alternative Medicine0，，： | 1 |
| 11 | Integrated electro-hydraulic system modeling and analysis显示文摘 | FU S J MARK L ISHAQUE S M | 2002 | IEEE AESS Systems Magazine2002，7，： | 1 |
| 12 | A deterministic particle swarm optimization maximum power point tracker for photovoltaic system under partial shading condition 显示文摘 | Ishaque K Salam Z | 2013 | IEEE Transactions on Industrial Electronics2013，60，8： | 1 |
| 13 | Simple, fast and accurate two-diode model for photovoltaic modules显示文摘 | Kashif Ishaque Zainal Salam Hamed Taheri | 2010 | Solar Energy Materials and Solar Cells2010，，2： | 1 |
| 14 | Role of Saceharomyces bou- lardii in diarrhea predominant irritable bowel syndrome 显示文摘 | Kabir MA Ishaque SM Ali MS | 2011 | Mymensingh Med J2011，20，3： | 1 |
| 15 | An improved particle swarm optimization(PSO)based MPPT for PV with reduced steady-state oscillation显示文摘 | Ishaque K Salam Z Amjad M | 2012 | IEEE Transactions on Power Electronics2012，27，8： | 1 |
| 16 | Effect of clipping stages on growth and herbage yield of Bluepanic grass 显示文摘 | Mushtaque M Ishaque M Ahmad M | 2009 | Pakistan Journal of Science2009，61，4： | 1 |
| 17 | Xylanase of Chaetomium cellulolyticum:its nature of production and hydrolytic potential显示文摘 | DUBEAU H CHAHAL D S ISHAQUE M | 1987 | Biotechnol Lett1987，9，： | 1 |
| 18 | A Greiner,Polymer,Metal,and Hybrid Nano-and Mesotubes by Coating Degradable Polymer Template Fibers显示文摘 | Bognitzki M Hou H Ishaque M | 2000 | Adv Mater2000，12，9： | 1 |
| 19 | A comprehensive Matlab simulink PV system simulator with partial shading capability based on two-diode model显示文摘 | Ishaque K Salam Z Syafaruddin | 2011 | Solar Energy2011，85，9： | 1 |
| 20 | Simple, fast and accurate two-di- ode model for photovoltaic modules显示文摘 | K Ishaque Z Salam H Taheri | 2011 | Solar Energy Materials and Solar Cells2011，95，2： | 1 |
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