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| 1 | Combination of epidural electrical stimulation with ex vivo triple gene therapy for spinal cord injury:a proof of principle study显示文摘Despite emerging contemporary biotechnological methods such as gene-and stem cell-based therapy,there are no clinically established therapeutic strategies for neural regeneration after spinal cord injury.Our previous studies have demonstrated that transplantation of genetically engineered human umbilical cord blood mononuclear cells producing three recombinant therapeutic molecules,including vascular endothelial growth factor(VEGF),glial cell-line derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)can improve morpho-functional recovery of injured spinal cord in rats and mini-pigs.To investigate the efficacy of human umbilical cord blood mononuclear cells-mediated triple-gene therapy combined with epidural electrical stimulation in the treatment of spinal cord injury,in this study,rats with moderate spinal cord contusion injury were intrathecally infused with human umbilical cord blood mononuclear cells expressing recombinant genes VEGF165,GDNF,NCAM1 at 4 hours after spinal cord injury.Three days after injury,epidural stimulations were given simultaneously above the lesion site at C5(to stimulate the cervical network related to forelimb functions)and below the lesion site at L2(to activate the central pattern generators)every other day for 4 weeks.Rats subjected to the combined treatment showed a limited functional improvement of the knee joint,high preservation of muscle fiber area in tibialis anterior muscle and increased H/M ratio in gastrocnemius muscle 30 days after spinal cord injury.However,beneficial cellular outcomes such as reduced apoptosis and increased sparing of the gray and white matters,and enhanced expression of heat shock and synaptic proteins were found in rats with spinal cord injury subjected to the combined epidural electrical stimulation with gene therapy.This study presents the first proof of principle study of combination of the multisite epidural electrical stimulation with ex vivo triple gene therapy(VEGF,GDNF and NCAM)for treatment of spinal cord injury in rat models.The animal protocols were approved by the Kazan State Medical University Animal Care and Use Committee(approval No.2.20.02.18)on February 20,2018. | Filip Olegovich Fadeev Farid Vagizovich Bashirov Vahe Arshaluysovich Markosyan Andrey Alexandrovich Izmailov Tatyana Vyacheslavovna Povysheva Mikhail Evgenyevich Sokolov Maxim Sergeevich Kuznetsov Anton Alexandrovich Eremeev Ilnur Ildusovich Salafutdinov Albert Anatolyevich Rizvanov Hyun Joon Lee Rustem Robertovich Islamov | 2021 | Neural Regeneration Research2021，16，3： | 4 |
| 2 | Comparison of data from registries of acute coronary syndromes RECORD and RECORD-2 :management of patients and its results in noninvasive hospitals 显示文摘 | Shevchenko II l:rlikh AD Islamov RR | 2013 | Kardiologiia2013，53，8： | 1 |
| 3 | Effect of bioresonance therapy on antioxidant system in lymphocytes in patients with rheumatoid arthritis显示文摘 | Islamov BI Balabanova RM Funtikov VA | 2002 | Bull Exp Biol Med2002，134，3： | 1 |
| 4 | Bioresonance therapy in rhenmatoid arthritis and heat,shock proteins显示文摘 | Islamov BI Funtikov VA Bobrovskii RV | 1999 | Biull Eksp Biol Med1999，128，11： | 1 |
| 5 | Structure-activity relationship for hemostatic lagochilin diterpenoids 显示文摘 | Zainutdinov U N Islamov R Dalimov D N | 2002 | Chem Nat Compd2002，38，2： | 1 |
| 6 | Effect of 17β-estradiol on gene expression in lumbar spinal cord following sciatic nerve crush injury in ovariectomized mice显示文摘 | Rustem R Islamov Wesley A Hendricks Laxmansa C Katwa | 2003 | Brain Research2003，966，1： | 1 |
| 7 | Using Markov reliability modelling for multiple repairable systems显示文摘 | Islamov R T | 1994 | Reliability Engineering and System Safety1994，，： | 1 |
| 8 | Effectof bioresonance therapy on antioxidant system in lymphocytesin patients with rheumatoid arthritis显示文摘 | Islamov BI Balabanova RM Funtikov VA | 2002 | Bull Exp BiolMed2002，134，3： | 1 |
| 9 | Effect of' bioresonance therapy on antioxidant sys- tem in lymphocytes in patients with rheumatoid arthritis显示文摘 | Islamov BI Balabanova RM Funtikov VA | 2002 | Bull Exp Biol Med2002，134，3： | 1 |
| 10 | Effect of bioresonance therapy on antioxidant system in lymphocytes in patients with rheumatoid arthritis 显示文摘 | Islamov BI | 2002 | Bull Exp Biol Med2002，134，3： | 1 |
| 11 | Transgenic maize plants expressing the Totivirus antifungal protein,KP4,are highly resistant to corn smut显示文摘 | Allen A Islamovic E Kaur J | | 0，，08： | 1 |
| 12 | Bioresonance therapy in rheumatoid arthritis and heat shock proteins 显示文摘 | Islamov BI Funtikov VA BobrovskiI RV | 1999 | Biull Ekxp Biol Med1999，128，11： | 1 |
| 13 | Transgenic maize plants expressing the Totivirus antifungal protein, KP4, are highly resistant to corn smut 显示文摘 | Alien A Islamovic E Kaur J | 2011 | Plant Biotechnology Journal2011，9，8： | 1 |
| 14 | Molecular and cellular changes in the post-traumatic spinal cord remodeling after autoinfusion of a genetically-enriched leucoconcentrate in a mini-pig model显示文摘Post-traumatic spinal cord remodeling includes both degenerating and regenerating processes,which affect the potency of the functional recovery after spinal cord injury(SCI).Gene therapy for spinal cord injury is proposed as a promising therapeutic strategy to induce positive changes in remodeling of the affected neural tissue.In our previous studies for delivering the therapeutic genes at the site of spinal cord injury,we developed a new approach using an autologous leucoconcentrate transduced ex vivo with chimeric adenoviruses(Ad5/35)carrying recombinant cDNA.In the present study,the efficacy of the intravenous infusion of an autologous genetically-enriched leucoconcentrate simultaneously producing recombinant vascular endothelial growth factor(VEGF),glial cell line-derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)was evaluated with regard to the molecular and cellular changes in remodeling of the spinal cord tissue at the site of damage in a model of mini-pigs with moderate spinal cord injury.Experimental animals were randomly divided into two groups of 4 pigs each:the therapeutic(infused with the leucoconcentrate simultaneously transduced with a combination of the three chimeric adenoviral vectors Ad5/35‐VEGF165,Ad5/35‐GDNF,and Ad5/35‐NCAM1)and control groups(infused with intact leucoconcentrate).The morphometric and immunofluorescence analysis of the spinal cord regeneration in the rostral and caudal segments according to the epicenter of the injury in the treated animals compared to the control mini-pigs showed:(1)higher sparing of the grey matter and increased survivability of the spinal cord cells(lower number of Caspase-3-positive cells and decreased expression of Hsp27);(2)recovery of synaptophysin expression;(3)prevention of astrogliosis(lower area of glial fibrillary acidic protein-positive astrocytes and ionized calcium binding adaptor molecule 1-positive microglial cells);(4)higher growth rates of regeneratingβIII-tubulin-positive axons accompanied by a higher number of oligodendrocyte transcription factor 2-positive oligodendroglial cells in the lateral corticospinal tract region.These results revealed the efficacy of intravenous infusion of the autologous genetically-enriched leucoconcentrate producing recombinant VEGF,GDNF,and NCAM in the acute phase of spinal cord injury on the positive changes in the post-traumatic remodeling nervous tissue at the site of direct injury.Our data provide a solid platform for a new ex vivo gene therapy for spinal cord injury and will facilitate further translation of regenerative therapies in clinical neurology. | Maria Aleksandrovna Davleeva Ravil Rasimovich Garifulin Farid Vagizovich Bashirov Andrei Aleksandrovich Izmailov Leniz Faritovich Nurullin Ilnur Ildusovich Salafutdinov Dilara Zilbarovna Gatina Dmitrij Nikolaevich Shcherbinin Andrei Aleksandrovich Lysenko Irina Leonidovna Tutykhina Maksim Mikhailovich Shmarov Rustem Robertovich Islamov | 2023 | Neural Regeneration Research2023，18，7： | 1 |
| 15 | 17β-estradiol stimulates regeneration of sciatic nerve in female mice 显示文摘 | Islamov RR Hendricks WA Jones RJ | 2002 | Brain Res2002，943，2： | 1 |
| 16 | Effect of 17β- estradiol on gene expression in lumbar spinal cord following sciatic nerve crush injury in ovarieetomized mice 显示文摘 | Islamov RR Hendricks WA Katwa LC | 2003 | Brain Res2003，966，1： | 1 |
| 17 | RNAi pathway is functional in peripheral nerve axons 显示文摘 | Murashov A K Chintalgattu V Islamov R R | 2007 | FASEB J2007，21，3： | 1 |
| 18 | Radiation-optical characteristics of quartz glass and sapphire显示文摘 | ISLAMOV A K IBRAGIMOVA E M NURITDINOV I | 2007 | Journal of Nuclear Materials2007，362，23： | 1 |
| 19 | High-throughput screening of hypothetical metal-organic frameworks for thermal conductivity显示文摘Thermal energy management in metal-organic frameworks(MOFs)is an important,yet often neglected,challenge for many adsorption-based applications such as gas storage and separations.Despite its importance,there is insufficient understanding of the structure-property relationships governing thermal transport in MOFs.To provide a data-driven perspective into these relationships,here we perform large-scale computational screening of thermal conductivity k in MOFs,leveraging classical molecular dynamics simulations and 10,194 hypothetical MOFs created using the ToBaCCo 3.0 code.We found that high thermal conductivity in MOFs is favored by high densities(>1.0 g cm^(−3)),small pores(<10Å),and four-connected metal nodes.We also found that 36 MOFs exhibit ultra-low thermal conductivity(<0.02 W m^(−1) K^(−1)),which is primarily due to having extremely large pores(~65Å).Furthermore,we discovered six hypothetical MOFs with very high thermal conductivity(>10 W m^(−1) K^(−1)),the structures of which we describe in additional detail. | Meiirbek Islamov Hasan Babaei Ryther Anderson Kutay B.Sezginel Jeffrey R.Long Alan J.H.McGaughey Diego A.Gomez-Gualdron Christopher E.Wilmer | 2023 | npj Computational Materials2023，，1： | 1 |
| 20 | An Analytical Model of the Ionic Wind in a Regular Ultracorona显示文摘 | Islamov R S | 2013 | Journal of Physics D:Applied Physics2013，46，： | 1 |
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