|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by retroviral vector显示文摘AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into incasingcells PA317 by lipofectamine mediated method,the clones of the cells transferred with hlL-6were selected by G418,and targeted HT-29 cellswere infected with the virus granules secretedfrom PA317 and also selected by G418.Test genetranscription and expression level byhybridization,ELISA and MTT assay,etc.Analyze tumor inhibitory effects according to thecell growth curve,plating forming rate andtumorigenicity in nude mice.RESULT Successfully constructed andtransfected recombinant expressing vectorspZIPIL-6 cDNA and got positive transfected celllines.The colon cancer cell line(HT-29 IL-5)transfected with the hlL-5 gene by retroviralvector was established.The log proliferationperiod and the doubling time of this cell line wasbetween 4 to 7 days and 2.5 days according tothe direct cell count,the cell proliferation wasobviously inhibited with MTT assay,the platinginhibitory rate was 50% by plating efficiencytest.When HT-29 IL-6 cells were inoculated intothe nude mice subcutaneously,carcinogenicactivity of the solid tumor was found superior tothe control group and the size of tumor was notsignificantly enlarged.Injection of combinationvirus fluid containing 11.-6 gene intotransplantation tumors could inhibit the growthand development of the tumor.CONCLUSION IL-6 could inhibit the growth andproliferation of colon cancer cells by retroviralvector-mediated transduction. | Xiao B Jing B Zhang YL Zhou DY Zhang WD | 2000 | World Journal of Gastroenterology2000，6，1： | 11 |
| 2 | Predictive role of GSTs on the prognosis of breast cancer patients with neoadjuvant chemotherapy 显示文摘 | Bai YL Zhou B Jing XY | 2012 | Asian Pac J Cancer Prev2012，13，10： | 1 |
| 3 | Apoptosis and growth inhibition in malignant lymphocytes after treatment with anenic trioxide at clinically achievable concentrations显示文摘 | Zhu XH Shen YL Jing YK | 1999 | J Natl Cancer Inst1999，91，6： | 1 |
| 4 | Use of statin during hospitalization im- proves the outcome after intracerebral hemorrhage 显示文摘 | Pan YS Jing J Wang YL | 2014 | C NS Neurosci T- her2014，20，： | 1 |
| 5 | Apoptosis and growth inhibition in malignant lymphocytes,after treatment with arsenic trioxide at clinically achievable concentrations显示文摘 | Shen YL Jing Y | 1999 | J Natl Cancer Inst1999，91，9： | 1 |
| 6 | Asenic trioxide induce malignant lymphocyte apoptosis and growth inhibition at clinically achievable concentrations显示文摘 | Shen YL Jing YK | 1999 | J Natl Cancer Inst1999，91，77： | 1 |
| 7 | Oral administration of lactococcus lactis delivered heat shock protein 65 attenuates atherosclerosis in low-density lipoprotein receptor-deficient mice 显示文摘 | Jing H Yong L Hai YL | 2011 | Vaccine2011，29，24： | 1 |
| 8 | Use of statin during hospitalization im- proves the outcome after intracerebral hemorrhage显示文摘 | Pan YS Jing J Wang YL | 2014 | CNS Neurosci T- her2014，20，： | 1 |
| 9 | Apoptosis and growth inhibition in malignant lymphocytes after treatment with arsenic trioxide at clinically achievable concentrations显示文摘 | Zhu XH Shen YL Jing YK | 1999 | J Natl Cancer Inst1999，91，9： | 1 |
| 10 | MicroRNA-9 promotes differentiation of mouse bone mesenehymal stem cells into neurons by Notch signaling 显示文摘 | Jing LJ Jia YL Lu JJ | 2011 | Neuroreport2011，22，5： | 1 |
| 11 | Detection of TERC amplification in cervical epithelial cells for the diagnosis of high-grade cervical lesions and invasive cancer显示文摘 | Jing J Wei LH Li YL | 2010 | J Mol Diagn2010，12，6： | 1 |
| 12 | Awaits that throat thicket is checked inner place the down carotid membrane to strip off the skill - attacks that 32 instances analyse contemporary显示文摘 | Zhu YL Jing GX Zhu D | 2004 | Chin J Mod Med2004，8，8： | 1 |
| 13 | Apoptosis and growth inhibition in malignant lymphocytes after treatment with arsenic trioxide at clinically achievable concentrations显示文摘 | Zhu XH Shen YL Jing YK | 1999 | J Natl Cancer Inst1999，91，9： | 1 |
| 14 | Evalua-tion of impact of Herba Erigerontis injection,a Chinese herbal pre-scription,on rat hepatic cytochrome P450enzymes by cocktail probe drugs显示文摘 | Han YL Li D Ren B Jing GP Meng XL Zhou ZY | 2012 | J Ethnopharmacol2012，139，1： | 1 |
| 15 | Sialic acid contributes to generation of ectopic spontaneous discharges in rats with neuropathic pain 显示文摘 | Zhang XL Peng XQ Jing YL | 2003 | Neurosci Lett2003，346，： | 1 |
| 16 | Effects of oocyte age, cumulus ceils and injection methods on in vitro development of intracytoplasmic sperm injection rabbit embryos 显示文摘 | Zheng YL Jing MX Zhang YL | 2004 | Zygote2004，12，： | 1 |
| 17 | Predictive role of GSTs on the prog- nosis of breast cancer patients with neoadjuvant chemotherapy显示文摘 | Bai YL Zhou B Jing XY | 2012 | Asian Pac J Cancer Prey2012，13，10： | 1 |
| 18 | Potassium-dependent folding: a key to intracellular delivery of G-quartet oligonucleotides as HIV inhibitors显示文摘 | Jing NJ Xiong WJ Guan YL | 2002 | Biochemistry2002，41，17： | 1 |
| 19 | Safety and efficacy of biodegaradable polymer-coated sirolimus-eluting stents in 'realworld' practice:18-month clinical and 9-month angiographic outcomes显示文摘 | HAN YL JING QM XU B | | 0，，02： | 1 |
| 20 | The efficacy and safety of pharmacoinvasive therapy with prourokinase for acute ST- segment elevation myocardial infarction patients with expected long percutaneous coronary intervention-related delay显示文摘 | Han YL Liu JN Jing Q M | 2013 | Cardiovasc Ther2013，31，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员