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| 1 | Clinical relationship between MDR1 gene and gallbladder cancer显示文摘BACKGROUND: The most common mechanisms of mul- tidrug resistance (MDR) in cancer cells is the expression of an energy-dependent exfflux pump. P-glycoprotein (P-gp) encoded by MDR1 gene and multidrug associated protein (MRP) are well known proteins associated with MDR. In human cancers, the MDR1 gene expression is common in patients with intrinsic and acquired MDR. It is a major therapeutic problem in cancer chemotherapy. Previously we found that the MDR of HCC is related to MRP gene ex- pression and initiates the intrinsic MDR. The aim of this study is to study the expression of MDR1 gene encoding P-gp and MDR1 mRNA in primary gallbladder carcinoma, and analyze its clinical significance. METHODS: Immunohistochemistry (IHC) S-P method and in situ polymerase chain reaction (ISPCR) were used to detect the expression of P-gp and MDR1 mRNA in 53 cases of untreated primary gallbladder carcinoma and 12 ca- ses of cholecystitis (archival paraffin-embedded tissues). RESULTS: The positive expression rates of P-gp and MDR1 mRNA in the 53 cases and 12 cases were 60.38%, 71.69% and 25.00%, 33.33%, respectively. There was a significant difference between the two groups (P<0.05). The positive expression rate of P-gp and MDRlmRNA were 69.44%, 83.33% and 41.18%, 47.06% respectively in tissues in stage of Nevin against Nevin , (P<0.05). In well, moderately differentiated gallbladder carcinoma tissues, their expressions were 79.49%, 69.23% against 50.00%, 35.71% in low, undifferentiated tissues (P<0.05). CONCLUSIONS: MDR to gallbladder carcinoma is closely related to the intrinsic MDR and it provides an important evidence to reverse the MDR by detection of the MDR1gene. Meanwhile, MDR1 gene expression in gallbladder carcinoma is correlated with some biological characteris- tics , takes part in the carcinogenesis of gallbladder tissues, and acts as a valuable biomarker of prognosis. | Bai-Lin Wang, Hai-Ying Zhai, Bing-Yi Chen, Shu-Ping Zhai, Hai-Yan Yang, Xiao-Ping Chen, Wen-Tao Zhao and Lei Meng Guangzhou, China Third Department of Surgery, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China Depart- ment of Surgery, First People’s Hospital, Jining 272100, China Department of Surgery, General Hospital of PLA, Beijing 100853, China and Hepatic Surgery Center, Tongji Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，2： | 9 |
| 2 | The effects of oleanolic acid on atherosclerosis in different animal models显示文摘 | Hanqiong Luo Jine Liu Qiong Ouyang Chunxiao Xuan Lanlan Wang Tingting Li Jun Liu | 2017 | Acta Biochimica et Biophysica Sinica2017，49，4： | 4 |
| 3 | Narrow-band interference excision in spread-spectrum systems using new N-sigma algorithm based on fuzzy threshold显示文摘The key to narrow-band interference excision in frequency domain is to determine the excision threshold in direct-sequence spread-spectrum (DS-SS) systems. The excision threshold is a non-linear function related to the number and the power of interference, and attempting to get the exact relation of threshold related to the number and the power of interference is almost impossible. The N-sigma algorithm determines the excision threshold using subsection function; however, the excision threshold determined by this method is not exact. A new method to determine the threshold of N-sigma algorithm is proposed. The new method modifies the scale factor N by use of the membership function. The threshold determined by this method is consecutive and smooth, and it is closer to the fact than that of the initial N-sigma algorithm. The GPS signal and single-tone (CW) interference (that is, typical narrow-band interference) are implemented in the simulation, and the results are presented to demonstrate the validity of the new algorithm. | Feng Jining Wu Siliang Wang Ju | 2009 | Journal of Systems Engineering and Electronics2009，20，1： | 4 |
| 4 | Circular dichroism-like response of terahertz wave caused by phase manipulation via all-silicon metasurface显示文摘Chiral metasurfaces based on asymmetric meta-atoms have achieved artificial circular dichroism(CD),spin-dependent wavefront control,near-field imaging,and other spin-related electromagnetic control.In this paper,we propose and experimentally verify a scheme for achieving high-efficiency chiral response similar to CD of terahertz(THz)wave via phase manipulation.By introducing the geometric phase and dynamic phase in an all-silicon metasurface,the spin-decoupled terahertz transmission is obtained.The giant circular dichroism-like effect in the transmission spectrum is observed by using a random phase distribution for one of the circular polarization components.More importantly,the effect can be adjusted when we change the area of the metasurface illuminated by an incident terahertz beam.In addition,we also demonstrate the spin-dependent arbitrary wavefront control of the transmitted terahertz wave,in which one of the circularly polarized components is scattered,while the other forms a focused vortex beam.Simulated and experimental results show that this method provides a new idea for spin selective control of THz waves. | Jie Li Chenglong Zheng Guocui Wang Jitao Li Hongliang Zhao Yue Yang Zhang Zhang Maosheng Yang Liang Wu Jining Li Yating Zhang Yan Zhang Jianquan Yao | 2021 | Photonics Research2021，9，4： | 3 |
| 5 | Predicting future dynamics from short-term time series using an Anticipated Learning Machine显示文摘Predicting time series has significant practical applications over different disciplines.Here,we propose an Anticipated Learning Machine(ALM)to achieve precise future-state predictions based on short-term but high-dimensional data.From non-linear dynamical systems theory,we show that ALM can transform recent correlation/spatial information of high-dimensional variables into future dynamical/temporal information of any target variable,thereby overcoming the small-sample problem and achieving multistep-ahead predictions.Since the training samples generated from high-dimensional data also include information of the unknown future values of the target variable,it is called anticipated learning.Extensive experiments on real-world data demonstrate significantly superior performances of ALM over all of the existing 12 methods.In contrast to traditional statistics-based machine learning,ALM is based on non-linear dynamics,thus opening a new way for dynamics-based machine learning. | Chuan Chen Rui Li Lin Shu Zhiyu He Jining Wang Chengming Zhang Huanfei Ma Kazuyuki Aihara Luonan Chen | 2020 | National Science Review2020，7，6： | 3 |
| 6 | Comparison of CO 2 emission scenarios and mitigation opportunities in China’s five sectors in 2020显示文摘 | Wenjia Cai Can Wang Jining Chen Ke Wang Ying Zhang Xuedu Lu | 2008 | Energy Policy2008，，3： | 3 |
| 7 | Identifying EGFR-Expressed Cells and Detecting EGFR Multi-Mutations at Single-Cell Level by Microfluidic Chip显示文摘EGFR mutations companion diagnostics have been proved to be crucial for the efficacy of tyrosine kinase inhibitor targeted cancer therapies. To uncover multiple mutations occurred in minority of EGFR-mutated cells,which may be covered by the noises from majority of unmutated cells, is currently becoming an urgent clinical requirement. Here we present the validation of a microfluidic-chip-based method for detecting EGFR multimutations at single-cell level. By trapping and immunofluorescently imaging single cells in specifically designed silicon microwells, the EGFR-expressed cellswere easily identified. By in situ lysing single cells, the cell lysates of EGFR-expressed cells were retrieved without cross-contamination. Benefited from excluding the noise from cells without EGFR expression, the simple and cost-effective Sanger's sequencing, but not the expensive deep sequencing of the whole cell population, was used to discover multi-mutations. We verified the new method with precisely discovering three most important EGFR drugrelated mutations from a sample in which EGFR-mutated cells only account for a small percentage of whole cell population. The microfluidic chip is capable of discovering not only the existence of specific EGFR multi-mutations,but also other valuable single-cell-level information: on which specific cells the mutations occurred, or whether different mutations coexist on the same cells. This microfluidic chip constitutes a promising method to promote simple and cost-effective Sanger's sequencing to be a routine test before performing targeted cancer therapy. | Ren Li Mingxing Zhou Jine Li Zihua Wang Weikai Zhang Chunyan Yue Yan Ma Hailin Peng Zewen Wei Zhiyuan Hu | 2018 | Nano-Micro Letters2018，10，1： | 2 |
| 8 | Enhancement of surface wettability via micro-and nanostructures by single point diamond turning显示文摘Studies on surface wettability have received tremendous interest due to their potential applications in research and industrial processes. One of the strategies to tune surface wettability is modifying surface topography at micro-and nanoscales. In this research, periodic micro-and nanostructures were patterned on several polymer surfaces by ultra-precision single point diamond turning to investigate the relationships between surface topographies at the micro-and nanoscales and their surface wettability. This research revealed that single-point diamond turning could be used to enhance the wettability of a variety of polymers, including polyvinyl chloride(PVC), polyethylene 1000(PE1000), polypropylene copolymer(PP) and polytetrafluoroethylene(PFTE), which cannot be processed by conventional semiconductor-based manufacturing processes. Materials exhibiting common wettability properties(θ≈ 90°) changed to exhibit 'superhydrophobic' behavior(θ > 150°). Compared with the size of the structures, the aspect ratio of the void space between micro-and nanostructures has a strong impact on surface wettability. | Nicolás Cabezudo Jining Sun Behnam Andi Fei Ding Ding Wang Wenlong Chang Xichun Luo Ben B.Xu | 2019 | Nanotechnology and Precision Engineering2019，2，1： | 2 |
| 9 | Development and analysis of a novel AF11-2 aptamer capable of enhancing the fluorescence of aflatoxin B1显示文摘Aflatoxin B1(AFB1)is one of the most common mycotoxins that threatens human health.As singlestranded oligonucleotides with high affinity and specificity,aptamers have incomparable effect on the targeted detection of AFB1.Herein,after 11 rounds of selection and analysis using a modified affinity chromatography-based SELEX strategy,the truncated 37 nt aptamer AF11-2 was successfully obtained.The aptamer shows good detection performance for AFB1,and can sensitively detect AFB1 in the range of 100-1000 nmol/L,with a detection limit of 42 nmol/L.In the detection of pretreated edible peanut oil samples,AF11-2 aptamer also showed a high recovery rate and good stability for AFB1,and achieved satisfactory results.In addition,AF11-2 aptamer can significantly enhance the fluorescence ability of AFB1,which is not available in traditional Afla17-2-3 aptamer.After molecular docking analysis,it was found that AF11-2 and Afla17-2-3 had different nucleotide binding sites for AFB1.Afla17-2-3 binds to the carbonyl O of AFB1,while AF11-2 binds to the pyrrolic O of AFB1,which may be the main reason that AF11-2 can enhance the fluorescence of AFB1. | Wenjing Li Yian Pei Jine Wang | 2022 | Chinese Chemical Letters2022，33，8： | 2 |
| 10 | Decomposition of energy-related CO 2 emission in China: 1957–2000显示文摘 | Can Wang Jining Chen Ji Zou | 2004 | Energy2004，，1： | 2 |
| 11 | Recalescence Velocity and Microstructure Evolution of Deeplyundercooled Cu_(65)Ni_(35) Alloy显示文摘The Cu_(65)Ni_(35) alloy liquid was undercooled by the fluxing method,and the rapid solidification structure was obtained by natural cooling.The solidification interface migration information of Cu_(65)Ni_(35) alloy liquid in rapid solidification stage was photographed with the help of high-speed camera,and the recalescence velocity was calculated.The microstructure evolution of the alloy was systematically studied by observing the microstructure morphology and taking photos on the metallographic microscope.By analyzing the evolution of dendrite grain size and microstructure microhardness with undercoolingand relying on electron backscatter diffraction(EBSD)technology,the grain refinement mechanism of microstructure under high undercooling and low undercooling is finally confirmed. | 梁海龙 CHENG Tang 李瑞琴 WANG Hongfu YANG Jine XIE Jinpeng 赵宇宏 | 2022 | Journal of Wuhan University of Technology(Materials Science)2022，37，2： | 2 |
| 12 | Scenario analysis on CO 2 emissions reduction potential in China’s iron and steel industry显示文摘 | Ke Wang Can Wang Xuedu Lu Jining Chen | 2006 | Energy Policy2006，，4： | 2 |
| 13 | Novel LFM Jammer Suppression Algorithm for DSSS in FRFT Domain显示文摘A novel algorithm is proposed to resolve the defects in the traditional linear frequency modulation(LFM)jamming suppression algorithm in the fractional Fourier transform(FRFT)domain.The traditional LFM jamming suppression algorithm in the FRFT domain has some defects.For example,the exact threshold is difficult to set and both the signal-to-noise ratio(SNR)loss and spectrum leakage are serious in strong jamming-to-signal ratio(JSR)situations.Windowing,overlapping and inverse windowing techniques are used in the FRFT domain to reduce the spectrum leakage and the SNR loss.Under the condition that only the direct sequence spread spectrum(DSSS)signal and white Gaussian noise are present,the amplitude of the received signal after FRFT is assumed to be Rayleigh distributed.Based on the distribution characteristic,hypothesis testing is used to suppress the jamming spectrum using the algorithm.Simulation results show that the performance of proposed algorithm is better than that of the conventional algorithms,especially in a strong jamming and multi component LFM jamming environment. | Jining Feng Xiaobo Yang Jun Wang | 2019 | Journal of Beijing Institute of Technology2019，28，4： | 2 |
| 14 | Expression of P-gp and miRNA-21 in Colorectal Cancer Tissues and the Clinical Significance显示文摘[Objectives]To study the expression of P-gp and miRNA-21 in colorectal cancer tissues and the relationship between the expression of P-gp and clinicopathological features,and to analyze the correlation between the expression of miRNA-21 and P-gp.[Methods]Immunohistochemical SP method was used to detect the expression level of P-gp in the tissues of 40 cases of colorectal cancer and the normal mucosal tissues 5 cm away from the corresponding cancer tissues,and the expression level of miRNA-21 in the paraffin tissues of the 40 cases was detected by RT-qPCR.[Results]The positive expression of P-gp was localized in the cell membrane,and the expression in colorectal cancer tissues was significantly higher than that in the corresponding adjacent tissues(P<0.05);the positive expression of P-gp was closely related to the degree of tumor differentiation and the depth of invasion(P<0.05),and the comparison results of P-gp expression in the differentiation groups of different cancer tissues showed that the expression level of P-gp in the moderately and poorly differentiated groups was significantly higher than that in the highly differentiated group(P<0.05),and the expression level of the moderately and poorly differentiated groups was similar(P>0.05).The expression of miRNA-21 in all cancer tissues was higher than that in the corresponding adjacent tissues(P<0.05),and the expression levels of miRNA-21 in differentiation groups of different cancer tissues were 1.286,1.445 and 1.992 times of those in the corresponding adjacent tissues,respectively;the comparison results of miRNA-21 between different differentiation groups showed that the expression level of miRNA-21 in the poorly differentiated group was significantly higher than that in the highly differentiated group and the moderately differentiated group(P<0.05),while the expression level of miRNA-21 in the highly differentiated group and the moderately differentiated group was similar(P>0.05).Spearman correlation analysis showed that there was a positive correlation on the expression between the two in colorectal cancer tissues(r=0.423,P<0.05).[Conclusions]The expression levels of miRNA-21 and P-gp are closely related to the differentiation degree of clinical colorectal cancer,the lower the differentiation degree,the higher the expression level of the two. | Xiaoli YAN Peng YAN Yanfang FAN Jing XUE Mingjuan WANG Jining ZHENG | 2020 | Medicinal Plant2020，11，6： | 1 |
| 15 | Decomposition of energy- related CO2 emission in China: 1957 - 2000 显示文摘 | Can Wang Jining Chen Ji Zou | 2005 | Energy2005 (30) : 73 - 832005，2005，30： | 1 |
| 16 | Decomposition of energy-re- lated CO2 emission in China: 1957-2000 显示文摘 | WANG Can CHEN Jining ZOU Ji | 2005 | Energy2005，，30： | 1 |
| 17 | Decomposition of energy- related CO2 emission in China : 1957- 2000 显示文摘 | WANG Can CHEN Jining ZOU Ji | 2005 | Energy2005，30，1： | 1 |
| 18 | Decomposition of Energy - related CO2 Emission in China: 1957 - 2000 显示文摘 | Wang Can Chen Jining Zou Ji | 2005 | Energy2005，，30： | 1 |
| 19 | Trend of technology innovation in China’s coal-fired electricity industry under resource and environmental constraints显示文摘 | Fanxian Yu Jining Chen Fu Sun Siyu Zeng Can Wang | 2010 | Energy Policy2010，，3： | 1 |
| 20 | Decomposition of energy-related CO2 emission in China:1957-2000显示文摘 | Wang Can Chen Jining ZOU Ji | 2005 | Energy2005，30，： | 1 |
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