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| 1 | Antibacterial activity of leaves and inter-nodal callus extracts of Mentha arvensis L显示文摘Objective:To determine the anti-bacterial efficacy of chloroform,ethanol,ethyl acetate and water extracts of inter-nodal and leaves derived calli extracts from Mentha arvensis(M.arvensis) against Salmonella typhi(S.typhi),Streptococcus pyogenes(S.pyogenes),Proteus vulgaris(P. vulgaris) and Bacillus subtilis(B.subtilis).Methods:The inter-nodal and leaves segments of M.arvensis were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-Dichlorophenoxyacetie acid(2,4-D) either alone or in combinations.The preliminary phytochemical screening was performed by Brindha et al method.Antibacterial efficacy was performed by disc diffusion method and incubated for 24 h at 37℃.Results:Maximum percentage of callus formation(inter-nodal segments 84.3±0.78;leaves segments 93.8±1.27) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 1.5 mg/L of 2,4-D.The ethanol extracts of leaves derived calli showed the maximum bio-efficacy than other solvents.The leaves and stem derived calli extracts on Proteus sp.showed that the plants can be used in the treatment of urinary tract infection associated with Proteus sp.Through the bacterial efficacy studies,it is confirmed that the in vitro raised calli tissue was more effective compared to in vivo tissue.Conclusions:The bio-efficacy study confirmed that the calli mediated tissues showed the maximum zone of inhibition.The present study paved a protocol to establish high potential cell lines by in vitro culture. | Johnson M Wesely EG Kavitha MS Uma V | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，3： | 9 |
| 2 | In vivo and in vitro phytochemical and antibacterial efficacy of Baliospermum montanum (Willd.) Muell.Arg.显示文摘Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in vitro derived rootlets and leaves segments of B.montanum were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-D either alone or in combinations.The preliminary phytochemical screening was performed by Harborne method.Antibacterial efficacy was performed by well diffusion method and incubated for 24 h at 37℃.Results:The highest percentage of callus formation(leaves segments 86.9±0.56:root segments 78.7±0.51) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 2.0 mg/L of 2.4-Dichlorophenoxy acetic acid.The phytochemical study revealed the high quantity presence of steroids,triterpenoids,glycosides,saponins,alkaloids,flavanoids, phenolic compounds,tannins,sugars etc of root and leaves derived calli.The ethanol extract of leaves segment derived calli of B.montanum showed the maximum solubility and antimicrobial activity with the MIC ranged from 100 to 200μL.Conclusions:The preliminary phytochemical study confirmed that the calli mediated tissues showed the higher percentage of metabolite constituents and extraction value compared to the in vivo leaves and roots.The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds. | Johnson M Wesely EG Zahir Hussain MI Selvan N | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，11： | 6 |
| 3 | The phospho- diesterase-5 inhibitor tadalafil reduces myocardial infarct size显示文摘 | SESTI C FLORIO V JOHNSON EG et ol | 2007 | Int J Impot Res2007，19，1： | 1 |
| 4 | The use of impra mine in depressed patients with congestive heart failure显示文摘 | Glassman AH Johnson LL Glardina EG | 1983 | JAMA1983，250，15： | 1 |
| 5 | Genotype-based dosing algorithms for warfarin therapy:data review and rec- ommendations显示文摘 | Johnson EG Home BD Carlquist JF | 2011 | Mol Diagn Ther2011，15，： | 1 |
| 6 | Rifampin protection against experimental graft sepsis显示文摘 | McDougal EG Burnham SJ Johnson G Jr | 1986 | J Vasc Surg1986，4，： | 1 |
| 7 | Assessment of passive c?ardiovascular implant devices for MRI compatibility显示文摘 | Walsh EG Brott BC Johnson VY et 01 | 2008 | Technol Health Care2008，16，4： | 1 |
| 8 | Human parasitization with Gordius robustus 显示文摘 | Sayad WY Johnson WM Faust EG | 1936 | J Amer Med Assn1936，106，6： | 1 |
| 9 | Nematocidal efficacy of eprinomectin, delivered topically, in naturally infected cattle显示文摘 | Yazwinski TA Johnson EG Thompson DR | 1997 | Vet Rec1997，58，6： | 1 |
| 10 | Transcatheter PFO closurein an aircrew member with cryptogenic stroke:aeromedicaldisposition显示文摘 | Johnson EG Mc Crary BF Kruyer WB | 2004 | Aviat Space Environ Med2004，75，2： | 1 |
| 11 | Pharmacology of riluzole in acute spinal cord injury 显示文摘 | Chow DS1 Teng Y Toups EG Aarabi B Harmp JS Shaffrey CI Johnson MM Boakye M Frankowski RF Fehlings MG Grossman RG | 2012 | J Neurosurg Spine2012，17，1： | 1 |
| 12 | Hemodialysis fistula occlusion : demonstration with 64-slice CT angiography显示文摘 | Neyman EG Johnson PT Fishman EK | 2006 | J Comput Assist Tomogr2006，30，1： | 1 |
| 13 | Transcatheter PFO closure in an aircrew member with cryptogenic stroke:aeromedical disposition显示文摘 | Johnson EG McCrary BF Kruyer WB | | 0，，02： | 1 |
| 14 | Assessment of passive c- ardiovascular implant devices for MRI compatibility显示文摘 | Walsh EG Brott BC Johnson VY | 2008 | Technol Health Care2008，16，4： | 1 |
| 15 | Positive patient outcome after manual cervical spine management despite a positive vertebral artery test 显示文摘 | Johnson EG Landel R Kusunose RS | 2008 | Man Ther2008，13，4： | 1 |
| 16 | Relationship between the du-plex doppler ultrasound and a questionnaire screening for positional tolerance of the cervical spine in subjects with suspected vascular pathology:a case series pilot study显示文摘 | Johnson EG Houle S Perez A | 2007 | J Man Manip Ther2007，15，4： | 1 |
| 17 | Social conflict exacerbates an animal model of multiple sclerosis显示文摘 | Meagher MW Johnson RR Vichaya EG | 2007 | Trauma Violence Abuse2007，8，3： | 1 |
| 18 | Cytochrome P450-catalyzed L-tryptophan nitration in thax- tominphytotoxin biosynthesis显示文摘 | Barry SM Kers JA Johnson EG | 2012 | Nature Chemical Biology2012，8，10： | 1 |
| 19 | Segmental neuropathic pain does not develop in male rats with complete spinal transections显示文摘 | Hubseher CH Kaddumi EG Johnson RD | 2008 | J Neurotrauma2008，25，10： | 1 |
| 20 | Hemodialysis fistula occlusion: demonstration with 64- slice CT angiography 显示文摘 | Neyman EG Johnson PT Fishman EK | 2006 | J Comput Assist Tomogr2006，30，： | 1 |
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