|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients显示文摘AIM To characterize peripheral blood natural killer(NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus(HIV)/hepatitis C virus(HCV) coinfected patients.METHODS Peripheral mononuclear cells from 24 HIV/HCV(HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: AntiCD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), antiNKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L(PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-Fas L(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using Flow Jo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained.RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was(6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls(G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1(G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035).CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. | Natalia Laufer Diego Ojeda María Laura Polo Ana Martinez Héctor Pérez Gabriela Turk Pedro Cahn Norberto Walter Zwirner Jorge Quarleri | 2017 | World Journal of Hepatology2017，9，25： | 2 |
| 2 | Improving the catalytic nitrate reduction 显示文摘 | Ulf P Marc H Jorg D | 2000 | Catalysis Today2000，55，： | 2 |
| 3 | DNA/RNA nucleotides and nucleosides:direct measurement of excited-state lifetimes by femtosecond fluorescence up-conversion显示文摘 | Peon Jorge Zewail Ahmed H | 2001 | Chemical Physics Letters2001，348，： | 2 |
| 4 | Effect of intensive treatment of hyperglycaemia on microvascular outcomes in type 2 diabetes: an analysis of the ACCORD randomised trial显示文摘 | Faramarz Ismail-Beigi Timothy Craven Mary Ann Banerji Jan Basile Jorge Calles Robert M Cohen Robert Cuddihy William C Cushman Saul Genuth Richard H Grimm Bruce P Hamilton Byron Hoogwerf Diane Karl Lois Katz Armand Krikorian Patrick O'Connor Rodica Pop-Bus | 2010 | The Lancet2010，，9739： | 2 |
| 5 | Value of the prednisolone test in my opathydiagnosis显示文摘 | Jorg J Gerhard H | 1984 | Fortschr NeurolPsychiatar1984，52，： | 1 |
| 6 | The Reverse Logistic Process of an Automobile Supply Chain Network Supported by a Collaborative Decision-making Model显示文摘 | JORGE E POLER H R MULA J | 2011 | Group Decis Negot2011，20，6： | 1 |
| 7 | Hydrogen cyanamide-induced budbreak and phytotoxicity in' Redhaven'peach buds显示文摘 | Jorge H Siller-Cepeda Leslie H | 1992 | Hort Science1992，27，8： | 1 |
| 8 | Effect of lameness on the calving- to-conception interval in dairy cows 显示文摘 | Jorge H Jan KS Daniel W W | 2001 | Journal of the American Veterinary Medical Association2001，218，10： | 1 |
| 9 | Value of the prednisolonetestin myopathy diagnosis显示文摘 | Jorg J Gerhard H | 1984 | Forrschr Neurol Psychiatar1984，52，1： | 1 |
| 10 | Aherosclerosis in type-2 Diabetes mellitus and insulin resistance: mechanistic links and therapeutic tar- gets 显示文摘 | Jorge P Giancarlo V Steven H | 2002 | Journal of Diabetes and its complications2002，16，6： | 1 |
| 11 | Reflective and transmissive CR scanhead technology on needle image plates显示文摘 | Jorg F Stephan M Clemens H | 2005 | SPIE2005，5745，： | 1 |
| 12 | The topology of complete minimal surfaces of finite total gaussian curvature 显示文摘 | JORGE L P M MEEKS III W H | 1983 | Topology1983，22，2： | 1 |
| 13 | Myocardial bridge显示文摘 | Jorge RA Joerg H David RH Jr | 2005 | European Heart J2005，26，12： | 1 |
| 14 | In vitro cytotoxic, anti protozoal and antimicrobial activities of medicinal plants from Vanu?atu显示文摘 | Gesine B Louis M Jorg H | 2010 | Phytother Res2010，24，： | 1 |
| 15 | Fine-mapping of the intestinal receptor locus for enterotoxigenic Eschefichia coli F4ac on porcine chromosome 13显示文摘 | Python P Jorg H Neuenschwander S | 2002 | Anim Genet2002，33，6： | 1 |
| 16 | Interventional MRA and intravascular imaging显示文摘 | Mare E L Harald H Q Jorg F D | 2000 | JMRI2000，12，： | 1 |
| 17 | Value of the prednisolonetestin myopathy disgnosis 显示文摘 | Jorg J Gerhard H | 1984 | Forrschr Neurol Psychiatar1984，52，： | 1 |
| 18 | Kinetic modeling of soot formation with detailed chemistry and physics: Laminar premixed flames of C2 hydrocarbons 显示文摘 | Jorg A Bockhorn H Frenklach M | 2000 | Combustion and Flame2000，121，12： | 1 |
| 19 | An approach to automated hardware/software partitioning using a flexible granularity that is driven by high-level estimation techniques 显示文摘 | JORG H ROLF E | 2001 | IEEE Transactions on Very Large Scale Integration (VLSI) Systems2001，9，2： | 1 |
| 20 | A multiple Objective Decision Support Model for the Selection of Remote Load Control Strategies显示文摘 | Jorge H Antunes C H Martins A G | 2000 | IEEE Transactions on Power Systems2000，5，15： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员