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| 1 | Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1显示文摘Ferroptosis is a form of regulated cell death, characterized by excessive membrane lipid peroxidation in an iron-and ROS-dependent manner. Celastrol, a natural bioactive triterpenoid extracted from Tripterygium wilfordii, shows effective anti-fibrotic and anti-inflammatory activities in multiple hepatic diseases. However, the exact molecular mechanisms of action and the direct protein targets of celastrol in the treatment of liver fibrosis remain largely elusive. Here, we discover that celastrol exerts anti-fibrotic effects via promoting the production of reactive oxygen species(ROS) and inducing ferroptosis in activated hepatic stellate cells(HSCs). By using activity-based protein profiling(ABPP) in combination with bio-orthogonal click chemistry reaction and cellular thermal shift assay(CETSA), we show that celastrol directly binds to peroxiredoxins(PRDXs), including PRDX1, PRDX2, PRDX4 and PRDX6,through the active cysteine sites, and inhibits their anti-oxidant activities. Celastrol also targets to heme oxygenase 1(HO-1) and upregulates its expression in activated-HSCs. Knockdown of PRDX1, PRDX2,PRDX4, PRDX6 or HO-1 in HSCs, to varying extent, elevated cellular ROS levels and induced ferroptosis. Taken together, our findings reveal the direct protein targets and molecular mechanisms via which celastrol ameliorates hepatic fibrosis, thus supporting the further development of celastrol as a promising therapeutic agent for liver fibrosis. | Piao Luo Dandan Liu Qian Zhang Fan Yang Yin-Kwan Wong Fei Xia Junzhe Zhang Jiayun Chen Ya Tian Chuanbin Yang Lingyun Dai Han-Ming Shen Jigang Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，5： | 14 |
| 2 | Nitrated and parent PAHs in the surface water of Lake Taihu,China:Occurrence,distribution,source,and human health risk assessment显示文摘Nitrated polycyclic aromatic hydrocarbons(NPAHs)have toxic potentials that are higher than those of their corresponding parent polycyclic aromatic hydrocarbons(PAHs)and thus have received increasing attention in recent years.In this study,the occurrence,distribution,source,and human health risk assessment of 15 NPAHs and 16 PAHs were investigated in the surface water from 20 sampling sites of Lake Taihu during the dry,normal,and flood seasons of 2018.The PAH concentrations ranged from 255 to 7298 ng/L and the NPAH concentrations ranged from not-detected(ND)to 212 ng/L.Among the target analytes,2-nitrofluorene(2-n Flu)was the predominant NPAH,with a detection frequency ranging from 85%to 90%and a maximum concentration of 56.2 ng/L.The three-ringed and four-ringed NPAHs and PAHs comprised the majority of the detected compounds.In terms of seasonal variation,the highest levels of the NPAHs and PAHs were in the dry season and flood season,respectively.Diagnostic ratio analysis indicated that the prime source of NPAHs was direct combustion,whereas in the case of PAHs the contribution was predominantly from a mixed pattern including pollution from unburned petroleum and petroleum combustion.The human health risk of NPAHs and PAHs was evaluated using a lifetime carcinogenic risk assessment model.The carcinogenic risk level of the targets ranged from 2.09×10^(-7)to 5.75×10^(-5)and some surface water samples posed a potential health risk. | Jijie Kong Yuxuan Dai Mengshu Han Huan He Jiapeng Hu Junyi Zhang Junzhe Shi Qiming Xian Shaogui Yang Cheng Sun | 2021 | Journal of Environmental Sciences2021，33，4： | 6 |
| 3 | Flavone Content and Bioactivity of Aqueous-Ethanolic Extracts of Phellinus Fruit Bodies显示文摘The flavone content of aqueous ethanolic extracts of Phellinus fruit bodies obtained from seven different locations was determined.Anti-tumor and antioxidant activities associated with the extracts were investigated in vitro using the Alamar Blue assay and a chemiluminecence-based method,respectively.Extracts of the seven Phellinus samples generated very similar HPLC profiles in qualitative terms although the areas of several peaks varied according to the sample type indicating quantitative differences in the case of some components.All the extracts,especially those of artificially cultivated Phellinus baumii fruit bodies,inhibited L1210 tumor cell proliferation and exhibited superoxide radical scavenging activity although there was no obvious correlation with flavone content. | HAO Ruixia ZHOU Shuai YANG Yan XI Junzhe LI Weiyao TANG Qingjiu WANG Chenguang | 2008 | 食用菌学报2008，15，2： | 6 |
| 4 | Ceria Nanoparticles as Enzyme Mimetics显示文摘 | Guohua Wang Junzhe Zhang Xiao He Zhiyong Zhang Yuliang Zhao | 2017 | Chinese Journal of Chemistry2017，35，6： | 5 |
| 5 | Experimental Study on Interaction Between Degrees of Freedom in a Wave Buoy显示文摘Increasing degrees of freedom(DOFs)is a useful way to raise the power capture efficiency of oscillating wave energy converters.Thus,this study proposes a buoy with three DOFs,which are surge,heave,and pitch.The hydrodynamic performance and power capture efficiency of the buoy is physically modeled.Amplitudes of unidirectional and coupled motions are compared to analyze the interaction effect between freedoms under conditions with and without power take-off damping.The capture width ratio and corresponding growth rates are also calculated.Results show that the buoy makes a periodic sinusoidal(or approximate)movement in every DOF.Coupling effect can cause an increase in the amplitude in one DOF and a decrease in the amplitudes of the others.This phenomenon shows that the kinematic energy of the buoy redistributes to all DOFs compared with the unidirectional conditions.Adding DOFs can improve the power absorption of the buoy in most cases,but the number of DOFs is not the more the better. | HUANG Shuting SHI Hongda CAO Feifei TAN Junzhe CHENG Haixun LI Demin LIU Shangze GONG Haoxiang TAO Ji | 2019 | Journal of Ocean University of China2019，18，6： | 5 |
| 6 | First-principles study of the effects of Si doping on geometric and electronic structure of closed carbon nanotube显示文摘The effects of Si doping on geometric and elec-tronic structure of closed carbon nanotube (CNT) are stud-ied by, a first-principles method, DMol. It is found that the local density of states at the Fermi level (EF) increases due to the Si-doping and the non-occupied states above the EF go down toward the lower energy range under an external elec-tronic field. In addition, due to the doping of Si, a sub-tip on the CNT cap is formed, which consisted of the Si atom and its neighbor C atoms. From these results it is concluded that Si-doping is beneficial to the CNT field emission properties. | ZHOU Junzhe WANG Chongyu | 2005 | Chinese Science Bulletin2005，50，17： | 5 |
| 7 | Negishi Coupling for Highly Selective Syntheses of Allenes via Ligand Effect and Mechanistic Study via SAESI-MS/MS显示文摘β-H elimination is an intrinsic problem in transition metal-catalyzed reactions.We describe herein an interesting ligand effect for Et2Zn acting as either ethyl provider or H provider,respectively:by applying SPhos or Gorlos-Phos as the ligand,β-H elimination has been successfully controlled in the corresponding Negishi coupling reaction affording different poly-substituted allenes in good yields and excellent selectivities.SAESI-MS (Solvent Assisted Electrospray Ionization Mass Spectrometry) has been applied to successfully capture the highly reactive organometallic intermediates,which show the different coordination behaviors of Pd with SPhos or Gorlos-Phos as the ligand in the catalytic cycle.In addition,the different reactivities of Int 1 and Int 2 towards the formation of the final allene products have been demonstrated via SAESI-MS/MS experiments.These MS studies visualized the whole catalytic cycle for the Negishi coupling reaction while nicely explain the observed reactivity and selectivity. | Yangguangyan Zheng Bukeyan Miao Anni Qin Junzhe Xiao Qi Liu Gen Li Li Zhang Fang Zhang Yinlong Guo Shengming Ma | 2019 | Chinese Journal of Chemistry2019，37，10： | 5 |
| 8 | 18beta-glycyrrhetinic acid induces ROS-mediated apoptosis to ameliorate hepatic fibrosis by targeting PRDX1/2 in activated HSCs显示文摘Hepatic stellate cells(HSCs)are essential drivers of fibrogenesis.Inducing activated-HSC apoptosis is a promising strategy for treating hepatic fibrosis.18beta-glycyrrhetinic acid(18b-GA)is a natural compound that exists widely in herbal medicines,such as Glycyrrhiza uralensis Fisch,which is used for treating multiple liver diseases,especially in Asia.In the present study,we demonstrated that 18b-GA decreased hepatic fibrosis by inducing the apoptosis in activated HSCs.18b-GA inhibited the expression of a-smooth muscle actin and collagen type Ⅰ alpha-1.Using a chemoproteomic approach derived from activity-based protein profiling,together with cellular thermal shift assay and surface plasmon resonance,we found that 18b-GA covalently targeted peroxiredoxin 1(PRDX1)and peroxiredoxin 2(PRDX2)proteins via binding to active cysteine residues and thereby inhibited their enzymatic activities.18b-GA induced the elevation of reactive oxygen species(ROS),resulting in the apoptosis of activated HSCs.PRDX1 knockdown also led to ROS-mediated apoptosis in activated HSCs.Collectively,our findings revealed the target proteins and molecular mechanisms of 18b-GA in ameliorating hepatic fibrosis,highlighting the future development of 18b-GA as a novel therapeutic drug for hepatic fibrosis. | Qian Zhang Piao Luo Liuhai Zheng Jiayun Chen Junzhe Zhang Huan Tang Dandan Liu Xueling He Qiaoli Shi Liwei Gu Jiahao Li Qiuyan Guo Chuanbin Yang Yin Kwan Wong Fei Xia Jigang Wang | 2022 | Journal of Pharmaceutical Analysis2022，12，4： | 3 |
| 9 | Effect of anti-freezing admixtures on alkali-silica reaction in mortars显示文摘 | Liu Junzhe Li Yushun Lv Lihua | 2005 | Journal of Wuhan University of Technology - Mater Sci Ed2005，，2： | 1 |
| 10 | Defect classification based on rectangular pulsed eddy current sensor in different directions显示文摘 | Yunze He Feilu Luo Mengchun Pan Xiangchao Hu Junzhe Gao Bo Liu | 2009 | Sensors & Actuators: A Physical2009，，1： | 1 |
| 11 | Short-term exposure to fine particulate matter constituents and mortality:case-crossover evidence from 32 counties in China显示文摘A growing number of studies associated increased mortality with exposures to specific fine particulate(PM_(2.5))constituents,while great heterogeneity exists between locations.In China,evidence linking PM_(2.5)constituents and mortality was extensively sparse.This study primarily aimed to quantify short-term associations between PM_(2.5)constituents and non-accidental mortality among the Chinese population.We collected daily mortality records from 32 counties in China between January 1,2011,and December 31,2013.Daily concentrations of main PM_(2.5)constituents(organic carbon(OC),elemental carbon(EC),nitrate(NO_(3)^(-)),sulfate(SO_(4)^(2−)),and ammonium(NH_(4)^(+)))were estimated using the modified Community Multiscale Air Quality model.Time-stratified case-crossover design with conditional logistic regression models was adopted to estimate mortality risks associated with short-term exposures to PM_(2.5)mass and its constituents.Stratification analyses were done by sex,age,and season.A total of 116,959 non-accidental deaths were investigated.PM_(2.5)concentrations on the day of death were averaged at 75.7μg m^(−3)(control day:75.6μg m^(−3)),with an interquartile range(IQR)of 65.2μg m^(−3).Per IQR rise in PM_(2.5),EC,OC,NO_(3)^(-),SO_(4)^(2−),and NH_(4)^(+)at lag-04 day was associated with an increase in non-accidental mortality of 2.4%(95%confidence interval,(1.0–3.7),1.7%(0.8–2.7),2.9%(1.6–4.3),2.1%(0.4–3.9),1.0%(0.2–1.9),and 1.6%(0.3–2.9),respectively.Both PM_(2.5)mass and its constituents were strongly associated with elevated cardiovascular mortality risks,but only PM_(2.5),EC,and OC were positively associated with respiratory mortality at lag-3 day.PM_(2.5)mass and its constituents associated effects on mortality varied among sex-and age-specific subpopulations.Differences in the seasonal pattern of associations exist among PM_(2.5)constituents,with stronger effects related to EC and NO_(3)^(-)in warm months but SO_(4)^(2−)and NH_(4)^(+)in cold months.Short-term exposures to PM_(2.5)compositions were positively associated with increased risks of mortality,particularly those constituents from combustion-related sources. | Peixuan Zhou Jianlin Hu Chuanhua Yu Junzhe Bao Siqi Luo Zhihao Shi Yang Yuan Shaocai Mo Zhouxin Yin Yunquan Zhang | 2022 | Science China(Life Sciences)2022，65，12： | 1 |
| 12 | Enzymatic synthesis of S-adenosyl-L-methionine on the preparative scale显示文摘 | Jeongho Park Junzhe Tai Charles A Roessner A Ian Scott | 1996 | Bioorgan & Medic chem1996，4，12： | 1 |
| 13 | A precise calculation of power system frequency显示文摘 | Yang Junzhe Liu Chih-Wen | 2001 | IEEE Trans on Power Delivery2001，16，3： | 1 |
| 14 | A new method for power signal harmonic analysis 显示文摘 | JunZhe Yang ChiShan Yu ChihWen Liu | 2005 | IEEE Transactions on Power Delivery2005，20，2： | 1 |
| 15 | Enzymatic synthesis of ,S-adenosyl-L-methionine on the preparative scale 显示文摘 | Park J Tai Junzhe Roessner C A | 1996 | Bioorg Med Chem1996，4，12： | 1 |
| 16 | Overcoming product inhibition of S-adenosyl-L methionine (SAM) synthetase: Preparation of SAM on the 30 mmol · L^-1 scale 显示文摘 | Park J Tai Junzhe Roessner C A | 1995 | Bioorg Med Chem1995，5，19： | 1 |
| 17 | Study on diffusion of nitrite ions in concrete and on protective effect of nitrite 显示文摘 | LIU JUNZHE RATHISH P KUMAR | 2006 | Indian Concrete Institute2006，7，4： | 1 |
| 18 | Effect of anti-freezing admixtures on alkali-silica reaction in mortars 显示文摘 | Liu Junzhe Li Yushun Lv Lihua | 2005 | Wuhan university of Technology-Mater Sei Ed2005，2，： | 1 |
| 19 | Enzymatic synthesis of 5-adenosyl-L-methionine on the preparative scale 显示文摘 | Park J Junzhe Tai Roessner C A | 1996 | Bioorg MedChem1996，4，12： | 1 |
| 20 | Overcoming productinhibition of S-adenosyl-L-methionine ( SAM ) synthetase :preparation of SAM on the 30 mmol/L Scale 显示文摘 | Park J Junzhe Tai Roessner C A | 1995 | Bioorg MedChem1995，5，19： | 1 |
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