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| 1 | Effects of exogenous recombinant human bone morphogenic protein-7 on the corneal epithelial mesenchymal transition and fibrosis显示文摘AIM:To evaluate the effect of exogenous recombinant human bone morphogenic protein-7(rhBMP-7)on transforming growth factor-β(TGF-β)-induced epithelial mesenchymal cell transition(EMT)and assessed its antifibrotic effect via topical application.METHODS:The cytotoxic effect of rhBMP-7 was evaluated and the EMT of human corneal epithelial cells(HECEs)was induced by TGF-β. HECEs were then cultured in the presence of rhBMP-7 and/or hyaluronic acid(HA). EMT markers,fibronectin,E-cadherin,α-smooth muscle actin(α-SMA),and matrix metaloproteinase-9(MMP-9),were evaluated. The level of corneal fibrosis and the reepithelization rate were evaluated using a rabbit keratectomy model. Expression of α-SMA in keratocytes were quantified following treatment with different concentrations of rhBMP-7.RESULTS:Treatment with rhBMP-7 attenuated TGF-β-induced EMT in HECEs. It significantly attenuated fibronectin secretion(31.6%; P<0.05),the α-SMA protein level(72.2%; P<0.01),and MMP-9 expression(23.6%,P<0.05)in HECEs compared with cells grown in the presence of TGF-β alone. E-cadherin expression was significantly enhanced(289.7%; P<0.01)in the presence of rhBMP-7. Topical application of rhBMP-7 combined with 0.1% HA significantly reduced the amount of α-SMA^+ cells by 43.18%(P<0.05)at a concentration of 2.5 μg/mL and by 47.73%(P<0.05)at 25 μg/mL,compared with the control group,without disturbing corneal reepithelization.CONCLUSION:rhBMP-7 attenuates TGF-β-induced EMT in vitro,and topical application of rhBMP-7 reduces keratocyte myodifferentiation during the early wound healing stages in vivo without hindering reepithelization. Topical rhBMP-7 application as biological eye drops seems to be feasible in diseases involving TGF-β-related corneal fibrosis with corneal reepithelization disorders. | Jin Kwon Chung Shin Ae Park Hee Sun Hwang Kwang Sung Kim Yang Je Cho Yong Sung You Young Sik Kim Ju Woong Jang Sung Jin Lee | 2017 | International Journal of Ophthalmology(English edition)2017，10，3： | 3 |
| 2 | Human amnion-derivedmesenchymal stemcells are a potential source foruterine stemcell therapy显示文摘 | Han K Lee JE Kwon SJ | 2008 | Cell Prolif2008，41，5： | 1 |
| 3 | A practical guide to wound heal- ing 显示文摘 | Janis JE Kwon RK LaLonde DH | 2010 | Plast Reconstr Surg2010，125，6： | 1 |
| 4 | Degradation of methylene blue via photocatalysis of titanium dioxide显示文摘 | Chul Han Kwon Hyunmin Shin Je Hun Kim | 2004 | Materials Chemistry and Physics2004，86，： | 1 |
| 5 | Preparation and characterization of TiO2-SiO2 nano - composite thin films 显示文摘 | Chul Han Kwon Je Hun Kim | 2003 | Ceramics International2003，29，8： | 1 |
| 6 | Ovarian cancer during pregnan-cy:clinical and pregnancy outcome显示文摘 | Kwon YS Mok JE Lim KT | 2010 | J Korean Med Sci2010，25，2： | 1 |
| 7 | Inhibition of nucleotide excision repair by high mobility group protein HMGA1 显示文摘 | Adair JE Kwon Y Dement GA | 2005 | Biol Chem2005，280，32： | 1 |
| 8 | Characterization of the murine cyclin-dependent kinase inhibitor gene P27kipl显示文摘 | Kwon TK Nagel JE Buchholz MA | | 0，，： | 1 |
| 9 | Paediatric brain-stem glio- mas: MRI, FDG-PET and histological grading correlation 显示文摘 | Kwon JW Kim IO Cheon JE | 2006 | Pediatr Radiol2006，36，9： | 1 |
| 10 | A novel angiotensin I converting enzyme inhibitory peptide from Alaska Pollack(Theragra chalcogramma) frame protein hydrolysate显示文摘 | Je JY Park PJ Kwon JY | 2004 | JAgric Food Chem2004，52，： | 1 |
| 11 | Antegrade recanalization of parent artery after internal trapping of ruptured vertebral artery dissecting aneurysm显示文摘 | Yon Kwon Ihn Jae Hoon Sung Hoon Byun JE | | 0，，： | 1 |
| 12 | Fine-needle aspiration cytology of abdo- minal wall endometriosis:a study of 10 cases显示文摘 | Kim JY Kwon JE Kim H J | 2013 | Diagn Cytopathol2013，41，2： | 1 |
| 13 | No ancillary finding is valid to distin- guish a primary ovarian hepatoid carcinoma from metastatic hepa- tocellular carcinoma 显示文摘 | Kwon JE Kim SH Cho NH | 2006 | Int J Gynecol Cancer2006，16，： | 1 |
| 14 | Degradation of methylene blue via photocatalysis of titanium dioxide显示文摘 | Chul Han Kwon Hyunmin Shin Je Hun Kim | 2004 | Materials Chemistry and Physics2004，86，1： | 1 |
| 15 | Comparative computation of orbital volume from axial and coronal CT using three-dimensional image analysis 显示文摘 | Kwon J Barrera JE Most SP | 2010 | Ophthal Plast Reconstr Surg2010，26，1： | 1 |
| 16 | Inhibition of nucleotide excision repair by high mobility group protein HMGA1显示文摘 | Adair JE Kwon Y Dement GA | | 0，，37： | 1 |
| 17 | Ovarian cancer during pregnan- cy: clinical and pregnancy outcome 显示文摘 | Kwon Y Mok JE Lim KT | 2010 | J Korean Med Sci2010，25，2： | 1 |
| 18 | The isoflavonoid aglyconerich fractions of Chungkookjiang,fermented unsalted soybeans,enchance insulin signaling and peroxisome proliferator-activated receptor-gamma activity in vitro显示文摘 | Kwon DY Jang JS Lee JE | | 0，，04： | 1 |
| 19 | Long thoracic nerve block in video-assisted thoracoscopic wedge resection for pneumothorax 显示文摘 | Kwon WK Choi JW Kang JE | 2012 | Anaesthesia and intensive care2012，40，5： | 1 |
| 20 | A Simple and Analytical Parameter- Extraction Method of a Microwave MOSFET 显示文摘 | Kwon I Je M Lee K | 2002 | IEEE Transac-tions on Microwave Theory & Techniques2002，50，6： | 1 |
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