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| 1 | Impact of copy number of distinct SV40PolyA segments on expression of a GFP reporter gene显示文摘The presence of Alu repeats downregulates the expression of the green fluorescent protein(GFP) gene.We found that SV40PolyA(PolyA,240 bp),in either orientation,eliminated the inhibition of GFP gene expression induced by Alu repeats when it was placed between the GFP gene and the Alu repeats.In this study,4 different segments(each 60 bp) were amplified from antisense PolyA(PolyAas) by PCR,and inserted upstream of Alu14 in pAlu14 plasmid(14 Alu repeats inserted downstream of the GFP gene in vector pEGFP-C1 in a head-tail tandem manner).Segments 1F1R(the first 60 bp segment at the 5' end of PolyAas) and 4F4R(the fourth 60 bp segment from the 5' end of PolyAas) did not activate GFP gene expression,whereas 2F2R and 3F3R(the middle two segments) did(as detected by Northern blot analysis and fluorescent microscopy).Different copy numbers of 2F2R and 3F3R segments,in a head and tail tandem manner,were inserted downstream of the GFP gene in pAlu14.p2F2R*4-Alu28,p3F3R*4-Alu18 and p3F3R*4-Alu28 were used as length controls to verify that the decrease in the expression of GFP was not due to the increased length of the inserted segment in the expression vectors.We found that 2 and 4 copies of 2F2R or 3F3R activated the GFP gene more strongly than one copy of them.However,more than 8 copies of 2F2R or 3F3R reduced the activation of the GFP gene.We concluded that SV40PolyAas contained at least two gene-activating elements(2F2R and 3F3R) and 2-4 copies of 2F2R or 3F3R were optimal for the expression of the GFP gene. | YIN Kang,WANG XiuFang,MA Huan,XIE Ying,FENG JingJing,YANG QinQing & Lü ZhanJun Hebei Key Lab of Laboratory Animal,Department of Genetics,Hebei Medical University,Shijiazhuang 050017,China | 2010 | Science China(Life Sciences)2010，53，5： | 2 |
| 2 | New synthesis of amphiphilic copolymers PE-g-PEO via esterfication显示文摘A new series of high molecular weight amphi-philic graft copolymers PE-g-PEO has been prepared. The esterifications between PE-p-MS-g-MA and PEO with OH group at the chain end are carried out under different condi-tions. The compositions of these graft copolymers are studied by NMR and FTIR. It is found to be a convenient and effi-cient way to prepare high molecular weight PE-g-PEO graft copolymers. | L?Yingying1, MA Zhi1,2, HU Youliang1, XU G. X.3 & CHUNG T. C.3 1. State Key Laboratory of Engineering Plastics, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Bei-jing Beijing Lab of Catalysis, Key Lab of Catalysis of China Na-tional Petroleum Corporation, Beijing 100080, China 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China 3. Department of Material Science and Engineering, Pennsylvania State University, University Park, PA 16802, USA | 2003 | Chinese Science Bulletin2003，48，6： | 2 |
| 3 | Purification and properties of primary and secondary alcohol dehydrogenases from Thermoanaerobacter ethanolicus显示文摘 | FRANK O B JUERGEN W LABS G L | 1988 | Applied and Environmental Microbology1988，54，2： | 1 |
| 4 | Pla sm a fibrinogen level and the risk of major cardiovascular diseases and nonvascular mortality:an individual participant Meta-analysis显示文摘 | Col lab or at ion FS Da ne sh J L ew i ng t on S | 2005 | JAMA2005，294，14： | 1 |
| 5 | Modafinil 显示文摘 | LAB L LAFON SA (FRANCE) | 1990 | Drugs Future1990，15，1： | 1 |
| 6 | Massive MIMO in the UL/DL of cellular networks : how many antennas do we need? 显示文摘 | Hoydis J Labs B Stuttgart A L | 2013 | IEEE Journal on Selected Areas in Communications2013，31，2： | 1 |
| 7 | Clinical significance of early thrombosis after prosthetic mitral valve replacement显示文摘 | Guillaume Laplace Stéphane Lafitte Jean-Noèl Labèque Jean-Marie Perron Eugène Baudet Claude Deville Xavier Roques Raymond Roudaut | 2004 | Journal of the American College of Cardiology2004，，： | 1 |
| 8 | A tutorial on hidden Markov models and selected applications in speech recognition显示文摘 | Rabiner L R AT&T Bell Lab Murray Hill | 1989 | Proceedings of the IEEE1989，77，2： | 1 |
| 9 | Coronary artery calcium score combined with Framingham score for risk prediction in asymptomatic individuals显示文摘 | Green land P LaB ree L Azen SP | 2003 | JAMA2003，291，2： | 1 |
| 10 | Laser surface alloying of aluminum (AA 1200) with Ni and SiC powders 显示文摘 | MABHALl LAB PITYANA S L SACKS N | 2010 | Materials and Manufacturing Processes2010，25，12： | 1 |
| 11 | ELECTRODE CHARACTERISTICS OF NANOCRYSTALLINE LaNi_5 PREPARED BY MECHANICAL ALLOYING显示文摘ELECTRODECHARACTERISTICSOFNANOCRYSTALLINELaNi5PREPAREDBYMECHANICALALLOYING①ChenZhenhua,ChenZhaohui,ZhouDuosanandHuangPeiyunIn... | Chen Zhenhua, Chen Zhaohui, Zhou Duosan and Huang PeiyunInstitute of Nonequilibrium Materials Science and EngineeringCentral South University of Technology, Changsha 410083, P.R. ChinaLü Manqi and Wang YulanState Key Lab for RSA, Institute of | 1998 | 中国有色金属学会会刊:英文版1998，8，2： | 0 |
| 12 | Calculation of fo and m_σ in a QCD-Based Model Field Theory | L Xiaofu~(1,2,4) Liu Yuxin~(1,3,4) Zhao Enguang~(1,4)1 CCAST(Warld Lab),P.O.Box 8730,Beijng 1000802 Department fo Physics,Sichuan,University,Chengdu 6100643 Department of Physics,Peking Uuiversity,Beijingg 1008714 Institute of Theoretical Physics,the Chinese Acodemy of Sciences,P.O.Box 2735,Beijing 100080 | 1996 | Chinese journal of nuclear physics1996，，3： | 0 |
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