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| 1 | Effects of penehyclidine hydrochloride in small intestinal damage caused by limb ischemia-reperfusion显示文摘AIM:To investigate the protective effect of penehyclidine hydrochloride post-conditioning in the damage to the barrier function of the small intestinal mucosa caused by limb ischemia-reperfusion(LIR) injury. METHODS:Male Wistar rats were randomly divided into three groups(36 rats each) :the sham-operation group(group S) ,lower limb ischemia-reperfusion group(group LIR) ,and penehyclidine hydrochloride postconditioning group(group PHC) .Each group was divided into subgroups(n=6 in each group) according to ischemic-reperfusion time,i.e.immediately 0 h(T1) ,1 h(T2) ,3 h(T3) ,6 h(T4) ,12 h(T5) ,and 24 h(T6) .Bilateral hind-limb ischemia was induced by rubber band application proximal to the level of the greater trochanter for 3 h.In group PHC,0.15 mg/kg of penehyclidine hydrochloride was injected into the tail vein immediately after 3 h of bilateral hind-limb ischemia.The designated rats were sacrificed at different time-points of reperfusion;diamine oxidase(DAO) ,superoxide dismutase(SOD) activity,myeloperoxidase(MPO) of small intestinal tissue,plasma endotoxin,DAO,tumor necrosis factor-α(TNF-α) ,and interleukin(IL) -10 in serum were detected in the rats. RESULTS:The pathological changes in the small intestine were observed under light microscope.The levels of MPO,endotoxin,serum DAO,and IL-10 at T1-T6,and TNF-αlevel at T1-T4 increased in groups LIR and PHC(P<0.05) compared with those in group S,but tissue DAO and SOD activity at T1-T6 decreased(P<0.05) .In group PHC,the tissue DAO and SOD activity at T2-T6,and IL-10 at T2-T5 increased to higher levels than those in group LIR(P<0.05) ;however,the levels of MPO,endotoxin,and DAO in the blood at T2-T6,and TNF-αat T2 and T4 decreased(P<0.05) . CONCLUSION:Penehyclidine hydrochloride post-conditioning may reduce the permeability of the small intestines after LIR.Its protection mechanisms may be related to inhibiting oxygen free radicals and inflammatory cytokines for organ damage. | Yan Zhang Yu-Fang Leng Xing Xue Yue Zhang Tao Wang Yu-Qing Kang | 2011 | World Journal of Gastroenterology2011，17，2： | 25 |
| 2 | Oxidative stress and hypoxia-induced factor 1α expression in gastric ischemia显示文摘AIM:To investigate the relation of reactive oxygen species (ROS) to hypoxia induced factor 1α (HIF-1α) in gastric ischemia. METHODS:The animal model of gastric ischemia reperfusion was established by placing an elastic rubber band on the proximal part of the bilateral lower limb for ligature for 3 h and reperfusion for 0,1,3,6,12 or 24 h. Ischemic post-conditioning,three cycles of 30-s reperfusion and 30-s femoral aortic reocclusion were conducted before reperfusion. Histological and immunohistochemical methods were used to assess the gastric oxidative damage and the expression of HIF1-α in gastric ischemia. The malondialdehyde (MDA) content and superoxide dismutase (SOD),xanthine oxidase (XOD) and myeloperoxidase (MPO) activities were determined by colorimetric assays. RESULTS:Ischemic post-conditioning can reduce post-ischemic oxidative stress and the expression of HIF-1α of gastric tissue resulting from limb ischemia reperfusion injury. MDA,SOD,XOD and MPO were regarded as indexes for mucosal injuries from ROS,and ROS was found to affect the expression of HIF-1α under gastric ischemic conditions. CONCLUSION:ROS affects HIF-1α expression under gastric ischemic conditions induced by limb ischemia reperfusion injury. Therefore,ROS can regulate HIF-1α expression in gastric ischemia. | Tao Wang Yu-Fang Leng Yan Zhang Xing Xue Yu-Qing Kang Yue Zhang | 2011 | World Journal of Gastroenterology2011，17，14： | 10 |
| 3 | World’s first spaceflight on-orbit demonstration of a flexible solar array system based on shape memory polymer composites显示文摘With a 10%reversible compressive strain in more than 10 deformation cycles,the shape memory polymer composites(SMPCs)could be used for deployable structure and releasing mechanism.In this paper,without traditional electro-explosive devices or motors/controllers,the deployable SMPC flexible solar array system(SMPC-FSAS)is studied,developed,ground-based tested,and finally on-orbit validated.The epoxy-based SMPC is used for the rolling-out variable-stiffness beams as a structural frame as well as an actuator for the flexible blanket solar array.The releasing mechanism is primarily made of the cyanate-based SMPC,which has a high locking stiffness to withstand 50 g gravitational acceleration and a large unlocking displacement of 10 mm.The systematical mechanical and thermal qualification tests of the SMPC-FSAS flight hardware were performed,including sinusoidal sweeping vibration,shocking,acceleration,thermal equilibrium,thermal vacuum cycling,and thermal cycling test.The locking function of the SMPC releasing mechanisms was in normal when launching aboard the SJ20 Geostationary Satellite on 27 Dec.,2019.The SMPC-FSAS flight hardware successfully unlocked and deployed on 5 Jan.,2020 on geostationary orbit.The triggering signal of limit switches returned to ground at the 139 s upon heating,which indicated the successful unlocking function of SMPC releasing mechanisms.A pair of epoxy-based SMPC rolled variable-stiffness tubes,which clapped the flexible blanket solar array,slowly deployed and finally approached an approximate 100%shape recovery ratio within 60 s upon heating.The study and on-orbit successful validation of the SMPC-FSAS flight hardware could accelerate the related study and associated productions to be used for the next-generation releasing mechanisms as well as space deployable structures,such as new releasing mechanisms with low-shocking,testability and reusability,and ultra-large space deployable solar arrays. | LAN Xin LIU LiWu ZHANG FengHua LIU ZhengXian WANG LinLin LI QiFeng PENG Fan HAO SiDa DAI WenXu WAN Xue TANG Yong WANG Mian HAO YanYan YANG Yang YANG Cheng LIU YanJu LENG JinSong | 2020 | Science China(Technological Sciences)2020，63，8： | 7 |
| 4 | Analysis of pore system model and physical property of coal reservoir in the Qinshui Basin显示文摘The Qinshui Basin in China is a major area for exploration and development of high rank coalbed methane. Due to the high rank coal and complicated pore system, no substantial breakthrough in the exploration and development of coalbed methane has been made until now. Many systematic tests show that a pore system of coal reservoir has some features as follows: the porosity is relatively low; the pore system is dominated by micropores and transition pores; mesopores take the second place, and macropores are nearly absent, which is exceedingly adverse for production of coal-bed methane. However, testing data also revealed the differential development for the pore of high rank coal reservoirs in the Qinshui Basin, which necessarily led to the different physical properties of desorption, diffusion and permeability. This paper classifies the testing data using cluster analysis method and selects the typical samples to establish four pore system models, analyzes the differences of reservoir physical property, and provides a guidance for the exploration and development of coalbed methane in the Qinshui Basin. | XU Hao ZHANG Shanghu LENG Xue TANG Dazhen WANG Mingshou | 2005 | Chinese Science Bulletin2005，50，S1： | 5 |
| 5 | The complete reference genome for grapevine (Vitis vinifera L.) genetics and breeding显示文摘Grapevine is one of the most economically important crops worldwide.However,the previous versions of the grapevine reference genome tipically consist of thousands of fragments with missing centromeres and telomeres,limiting the accessibility of the repetitive sequences,the centromeric and telomeric regions,and the study of inheritance of important agronomic traits in these regions.Here,we assembled a telomere-to-telomere(T2T)gap-free reference genome for the cultivar PN40024 using PacBio HiFi long reads.The T2T reference genome(PN_T2T)is 69 Mb longer with 9018 more genes identified than the 12X.v0 version.We annotated 67%repetitive sequences,19 centromeres and 36 telomeres,and incorporated gene annotations of previous versions into the PN_T2T assembly.We detected a total of 377 gene clusters,which showed associations with complex traits,such as aroma and disease resistance.Even though PN40024 derives from nine generations of selfing,we still found nine genomic hotspots of heterozygous sites associated with biological processes,such as the oxidation–reduction process and protein phosphorylation.The fully annotated complete reference genome therefore constitutes an important resource for grapevine genetic studies and breeding programs. | Xiaoya Shi Shuo Cao Xu Wang Siyang Huang Yue Wang Zhongjie Liu Wenwen Liu Xiangpeng Leng Yanling Peng Nan Wang Yiwen Wang Zhiyao Ma Xiaodong Xu Fan Zhang Hui Xue Haixia Zhong Yi Wang Kekun Zhang Amandine Velt Komlan Avia Daniela Holtgräwe Jérôme Grimplet JoséTomás Matus Doreen Ware Xinyu Wu Haibo Wang Chonghuai Liu Yuling Fang Camille Rustenholz Zongming Cheng Hua Xiao Yongfeng Zhou | 2023 | Horticulture Research2023，10，5： | 3 |
| 6 | Global gene expression profiles for the growth phases of Trichophyton rubrum显示文摘Trichophyton rubrum (T.rubrum) is a common superficial fungus.Molecular and genetic studies of T.rubrum are still limited.In this paper,we report the global analysis of gene expression profiles at different growth phases using cDNA microarray technology.A total of 2044 differentially expressed genes were obtained and clustered into three expression patterns.Our data confirmed previous results that many mRNAs were pre-stored in the conidia of T.rubrum.Transcriptional profiling and function analysis showed that some glycolytic enzymes share similar expression patterns and may be coregulated during the transition of growth phases.Some genes involved in small GTPase signaling pathways,and in cAMP-dependent and MAPK regulation pathways were induced in response to the growth dynamics of T.rubrum.Although the detailed biological roles of these T.rubrum genes are still unknown,our results suggest that these genes may be involved in regulation mechanisms in the life cycle of the fungus. | XU XingYe LIU Tao LENG WenChuan DONG Jie XUE Ying YANG HanChuna JIN Qi | 2011 | Science China(Life Sciences)2011，54，7： | 2 |
| 7 | Tribocorrosion behavior of DLC-coated CoCrMo alloy in simulated biological environment显示文摘 | Jia Liu Xue Wang B.J. Wu T.F. Zhang Y.X. Leng Nan Huang | 2013 | Vacuum2013，，： | 2 |
| 8 | Similar or different: the same Spiro-core but different alkyl chains with apparently improved device performance of perovskite solar cells显示文摘By intelligently utilizing the odd-even effect existing in the melting points of alkanes as presented in the basic textbook of Organic Chemistry, different alkoxy groups were introduced to modify the structure of commercial Spiro-OMeTAD to give new Spiro derivatives of Spiro-OEtTAD, Spiro-OPrTAD, Spiro-OiPrTAD and Spiro-OBuTAD, with the aim to adjust the molecular packing status in perovskite solar cells as hole transporting compounds. Excitedly, with the introduction of ethoxy groups instead of the methoxy ones in Spiro-OMeTAD, Spiro-OEtTAD-based perovskite solar cells demonstrated the highest device performance of 20.16%, higher than that of Spiro-OMeTAD(18.64%). | Fan Liu Shiqing Bi Xiaorui Wang Xuanye Leng Mengmeng Han Baoda Xue Qianqian Li Huiqiong Zhou Zhen Li | 2019 | Science China Chemistry2019，62，6： | 2 |
| 9 | Protocol on transcranial alternating current stimulation for the treatment of major depressive disorder:a randomized controlled trial显示文摘Background:Transcranial alternating current stimulation(tACS)offers a new approach for adult patients with major depressive disorder(MDD).The study is to evaluate the efficacy and safety of tACS treating MDD.Methods:This is an 8-week,double-blind,randomized,placebo-controlled study.Ninety-two drug-naive patients with MDD aged 18 to 65 years will receive 20 daily 40-min,77.5-Hz,15-mA sessions of active or sham tACS targeting the forehead and both mastoid areas on weekdays for 4 consecutive weeks(week 4),following a 4-week observation period(week 8).The primary outcome is the remission rate defined as the 17-item Hamilton depression rating scale(HDRS-17)score≤7 at week 8.Secondary outcomes are the rates of response at weeks 4 and 8 and rate of remission at week 4 based on HDRS-17,the proportion of participants having improvement in the clinical global impression-improvement,the change in HDRS-17 score(range,0-52,with higher scores indicating more depression)over the study,and variations of brain imaging and neurocognition from baseline to week 4.Safety will be assessed by vital signs at weeks 4 and 8,and adverse events will be collected during the entire study.Discussion:The tACS applied in this trial may have treatment effects on MDD with minimal side effects.Trial registration:Chinese Clinical Trial Registry,ChiCTR1800016479;http://gffzz62a03ee25e6f4a77h0owxbuk6fq0o6pq6.ffgz.tsg.suse.edu.cn/showproj.aspx?proj=22048. | Hong-Xing Wang Kun Wang Wen-Rui Zhang Wen-Feng Zhao Xiao-Tong Yang Li Wang Mao Peng Zhi-Chao Sun Qing Xue Yu Jia Ning Li Kai Dong Qian Zhang Shu-Qin Zhan Bao-Quan Min Chun-Qiu Fan Ai-Hong Zhou Hai-Qing Song Lu Yin Tian-Mei Si Jing Huang Jie Lu Hai-Xia Leng Wei-Jun Ding Yuan Liu Tian-Yi Yan Yu-Ping Wang | 2020 | Chinese Medical Journal2020，，1： | 2 |
| 10 | Analysis of part of the Trichophyton rubrum ESTs显示文摘Trichophyton rubrum (T. rubrum) is the most common of the superficial fungi. In an effort to better understand the genetic and biochemical makeup of T. rubrum, we generated cDNA libraries from 3 growth stages and used these to isolate 4002 unique expressed sequence tags (ESTs). Sequence comparisons with the Genbank database allowed 1226 of the ESTs to be assigned putative functions or matched with homologs from other organisms. Of the remaining ESTs, 989 were only weakly similar to known sequences and 1787 had no identifiable functions, suggesting that they represent novel genes. We further analyzed the presence of several im-portant genes involved in the growth, metabolism, signal transduction, pathogenesis and drug resistance in T. rubrum. This information was used to newly elucidate important metabolic path-ways in T. rubrum. Taken together, our results should form the molecular basis for continued re-search on the physiological processes and pathogenic mechanisms of T. rubrum, and may lead to a better understanding of fungal drug resistance and identification of new drug targets. | WANG Lingling1*, MA Li1,*,, LENG Wenchuan1, YANG Jian2, ZHU Junping1, DONG Jie1, XUE Ying1, WAN Zhe3, LI Ruoyu3 & JIN Qi1 1. State Key Laboratory For Molecular Virology and Genetics Engineering, Beijing 100176, China 2. Chinese National Human Genome Center, Beijing, Beijing 100176, China 3. Research Centre for Medical Mycology, Beijing 100034, China | 2004 | Science China(Life Sciences)2004，47，5： | 2 |
| 11 | Baseline total and specific differential white blood cell counts and 5-year allcause mortality in community-dwelling older women显示文摘 | Leng S X Xue Q L Huang Y et d | 2005 | Exp Gerontol2005，40，12： | 1 |
| 12 | Two-Dimensional Variational Analysis of Near-Surface Moisture from Simulated Radar Refractivity-Related Phase Change Observations显示文摘Because they are most sensitive to atmospheric moisture content,radar refractivity observations can provide high-resolution information about the highly variable low-level moisture field.In this study,simulated radar refractivity-related phase-change data were created using a radar simulator from realistic highresolution model simulation data for a dryline case.These data were analyzed using the 2DVAR system developed specifically for the phase-change data.Two sets of experiments with the simulated observations were performed,one assuming a uniform target spacing of 250 m and one assuming nonuniform spacing between 250 m to 4 km.Several sources of observation error were considered,and their impacts were examined.They included errors due to ground target position uncertainty,typical random errors associated with radar measurements,and gross error due to phase wrapping.Without any additional information,the 2DVAR system was incapable of dealing with phase-wrapped data directly.When there was no phase wrapping in the data,the 2DVAR produced excellent analyses,even in the presence of both position uncertainty and random radar measurement errors.When a separate pre-processing step was applied to unwrap the phase-wrapped data,quality moisture analyses were again obtained,although the analyses were smoother due to the reduced effective resolution of the observations by interpolation and smoothing involved in the unwrapping procedure.The unwrapping procedure was effective even when significant differences existed between the analyzed state and the state at a reference time.The results affirm the promise of using radar refractivity phase-change measurements for near-surface moisture analysis. | Ken-ichi SHIMOSE Ming XUE Robert D.PALMER Jidong GAO Boon Leng CHEONG David J.BODINE | 2013 | Advances in Atmospheric Sciences2013，30，2： | 1 |
| 13 | Highly flexible transparent and conductive ZnS/Ag/ZnS multilayer films prepared by ion beam assisted deposition显示文摘 | Yu Z Leng J Xue W | | 0，，： | 1 |
| 14 | Highly flexible transparent and conductive ZnS/Ag/ZnS multilayer films prepared by ion beam assisted deposition 显示文摘 | Yu Zhinong Leng Jian Xue Wei | 2012 | Appl Surf Sci2012，258，7： | 1 |
| 15 | Factors influencing outcomes after cardiopulmonary resuscitation in emergency department显示文摘BACKGROUND:The outcome of cardiopulmonary resuscitation(CPR) may depend on a variety of factors related to patient status or resuscitation management.To evaluate the factors influencing the outcome of CPR after cardiac arrest(CA) will be conducive to improve the effectiveness of resuscitation.Therefore,a study was designed to assess these factors in the emergency department(ED) of a city hospital.METHODS:A CPR registry conforming to the Utstein-style template was conducted in the ED of the First Affiliated Hospital of Wenzhou Medical College from January 2005 to December 2011.The outcomes of CPR were compared in various factors groups.The primary outcomes were rated to return of spontaneous circulation(ROSC),24-hour survival,survival to discharge and discharge with favorable neurological outcomes.Univariate analysis and multivariable logistic regression analysis were performed to evaluate factors associated with survival.RESULTS:A total of 725 patients were analyzed in the study.Of these patients,187(25.8%) had ROSC,100(13.8%) survived for 24 hours,48(6.6%) survived to discharge,and 23(3.2%) survived to discharge with favorable neurologic outcomes.A logistic regression analysis demonstrated that the independent predictors of ROSC included traumatic etiology,first monitored rhythms,CPR duration,and total adrenaline dose.The independent predictors of 24-hour survival included traumatic etiology,cardiac etiology,first monitored rhythm and CPR duration.Previous status,cardiac etiology,first monitored rhythms and CPR duration were included in independent predictors of survival to discharge and neurologically favorable survival to discharge.CONCLUSIONS:Shockable rhythms,CPR duration ≤15 minutes and total adrenaline dose≤5 mg were favorable predictors of ROSC,whereas traumatic etiology was unfavorable.Cardiac etiology,shockable rhythms and CPR duration ?15 minutes were favorable predictors of 24-hour survival,whereas traumatic etiology was unfavorable.Cardiac etiology,shockable rhythms,CPR duration ≤15 minutes were favorable predictors of survival to discharge and neurologically favorable survival to discharge,but previous terminal illness or multiple organ failure(MOF) was unfavorable. | Ji-ke Xue Qiao-yun Leng Yu-zhi Gao Shou-quan Chen Zhang-ping Li Hui-ping Li Wei-jia Huang Jun-yan Cheng Jie Zhang Ai-wen He | 2013 | World Journal of Emergency Medicine2013，4，3： | 1 |
| 16 | Phylogenetic and molecular characterization of coxsackievirus A24variant isolates from a 2010acute hemorrhagic conjunctivitis outbreak in Guangdong,China显示文摘 | Wu De Zheng Huanying Li Hui Monagin Corina Guo Xue Liu Leng Zeng Hanri Fang Ling Mo Yanling Zhou Huiqiong Zhang Huan Kou Jing Long Caiyun Hiromu Yoshida and Ke Changwen | 2012 | Virol J2012，9，41： | 1 |
| 17 | Mutations in the genome of the highly pathogenic porcine reproductive and re- spiratory syndrome virus potentially related to attenuation显示文摘 | Leng Xue Li Zhenguang Xia Mingqi | 2012 | Vet Microbiol2012，57，12： | 1 |
| 18 | Highly flexible transparent and conductive ZnS/Ag/ZnS multilayer films prepared by ion beam assisted deposition显示文摘 | Zhinong Yu Jian Leng Wei Xue | 2012 | Applied Surface Science2012，2587，： | 1 |
| 19 | Molecular Diagnosis of Human Cancer Type by Gene Expression Profiles and Independent Component Analysis显示文摘 | Zhang Xue Wu Yap Yee Leng Wei Dong | 2005 | European Journal of Human Genetics2005，5，9： | 1 |
| 20 | Mutations in the ge- nome of the highly pathogenic porcine reproductive and respir- atory syndrome virus potentially related to attenuation显示文摘 | Xue Leng Zhen G L Ming Q X | 2012 | Vet Microl2012，157，12： | 1 |
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