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| 1 | Establishment of a high-throughput screening system for universal anti-HIV targets显示文摘The process of identifying novel human immunodeficiency virus 1 (HIV-1) inhibitors presents a challenge for industrial and scientific research.Virus-cell-based screening approaches offer some advantages in the quest for novel inhibitors because they include multiple targets in a single screen and in some cases reveal targets not captured in biochemical assays.In this study,a high-throughput screening (HTS) system for HIV-1 inhibitors was developed,which allows the simultaneous screening of all the HIV-1 targets required for replication in the cell culture.HeLa/cluster of differentiation 4 (CD4)/long terminal repeat (LTR) indicator cells,which stably expressed high levels of HIV receptor CD4 and contained the firefly luciferase reporter gene under the control of the HIV-1 LTR promoter,were generated.The expression of CD4 and LTR function in this cell line was validated by Western blot and luciferase assay.MT2 cells,a human T-cell leukemia cell line that support high levels of HIV-1 replication,were infected with HIV-1,and then the infected MT2 cells were co-cultured with HeLa/CD4/LTR cells.In the optimized assay conditions,HIV-1 replication occurs rapidly in the MT2 cells,resulting in the infection of the HeLa/CD4/LTR cells and a significant induction of luciferase signals through LTR,which is activated by the expression of HIV-1 tat gene.The luciferase signals of HeLa/CD4/LTR cells co-cultured with HIV-1 infected MT2 cells were significantly stronger than the signals of noninfected HeLa/CD4/LTR cells (P < 0.001).The inhibitory effects of HIV-1 inhibitors (3'-Azido-3'-deoxythymidine [AZT],efavirenz [EFV],and nevirapine [NVP]) were evaluated with this assay,and the inhibitory concentration 50% (IC50) values of the above three inhibitors were 58,1.4,and 85 nmol/L,respectively,indicating that the assay provides the necessary sensitivity for identifying antiviral molecules.The Z' factor had a value of 0.563,indicating this is a very robust assay.These results suggested that HIV-1 infection assay represents a novel approach to HIV-1 antiviral screening that allows for the effective execution of HTS campaigns. | YIN Qi ZHUANG DaoMing JIANG YaQin ZHAO ChuanKe ZENG Xin LI ShiYou | 2010 | Chinese Science Bulletin2010，55，10： | 3 |
| 2 | A New Model for the Characterization of Frozen Soil and Related Latent Heat Effects for the Improvement of Ground Freezing Techniques and Its Experimental Verification显示文摘The correct determination of thermal parameters,such as thermal conductivity and specific heat of soil during freezing,is the most important and basic problem for the construction of an appropriate freezing method.In this study,a calculation model of three stages of soil temperature was established.At the unfrozen and frozen stages,the specific temperatures of dry soil,water,and ice are known.According to the principle of superposition,a calculation model of unfrozen and frozen soils can be established.Informed by a laboratory experiment,the latent heat of the adjacent zone was calculated for the freezing stage based on different water contents in the temperature section.Both the latent and specific heat of water,ice,and particles were calculated via superposition of the weight percentage content.A calculation model of the specific heat of the freezing stage was built,which provides both guidance and theoretical basis for the calculation of the specific heat of frozen soil. | Daoming Shen Hua Si Jinhong Xia Shunqun Li | 2019 | Fluid Dynamics & Materials Processing2019，15，1： | 2 |
| 3 | Perspectives on diacylglycerol-induced improvement of insulin sensitivity in type 2 diabetes显示文摘Diacylglycerol(DAG)-based edible oils have attracted increasing research interest owing to their healthpromoting properties.Recent animal and human studies showed that an increased 1,2-DAG content in the liver and skeletal muscle may cause insulin resistance.However,earlier studies using animal models or humans reported that dietary DAGs with a 1,2-DAGs to 1,3-D AGs ratio of approximately 3:7 could improve insulin sensitivity in type 2 diabetic patients.This conflict raises the question of whether there is a link between the ingested DAGs and endogenous DAGs during their metabolism.To make a contribution to this field,this review provides an overview of the metabolic pathways of ingested DAGs and biological roles of DAGs(ingested and endogenous)in the change of insulin sensitivity.Accordingly,strategies for further investigations on the metabolism of DAGs are proposed. | Daoming Li Yang Zhu Yonghua Wang Qiong Zou Jinzhu Duan Dongxiao Sun-Waterhouse Baoguo Sun | 2022 | Food Science and Human Wellness2022，11，2： | 2 |
| 4 | Pitting corrosion behaviour of stainless steel in ultrasonic cell 显示文摘 | SUN Daoming JIANG Yiming TANG Yu XIANG Qiuwei ZHONG Cheng LIAO Jiaxing LI Jin | 2009 | Electrochimica Acta2009，54，： | 1 |
| 5 | Influence of annealing treatment on the corrosion resistance of lean duplex stainless steel 2101显示文摘 | ZHANG Lihua ZHANG Wei JIANG Yiming DENG Bo SUN Daoraing LI Daoming | 2009 | Electrochimiea Acta2009，54，： | 1 |
| 6 | Tensile deformation behavior of aluminum alloy at warm forming temperature 显示文摘 | Li Daoming Amit Ghosh | 2003 | Materials Science and Engineering A2003，352，： | 1 |
| 7 | Abnormal growth transport in oxide scales on Fe-16Cr steels in water vapor显示文摘 | GONG Jia JIANG Yiming DENG Bo ZHONG Cheng SUN Daoming LI Jin | 2009 | Metall Mater Trans A2009，，： | 1 |
| 8 | Biaxial warm forming behavior of aluminum sheet alloys 显示文摘 | Li Daoming Amit Ghosh | 2004 | Journal of Materials Processing Technology2004，145，： | 1 |
| 9 | Tensile deformation behavior of aluminum alloys at warm forming temperatures 显示文摘 | Li Daoming Ghosh Amit | 2003 | Materials Science and Engineering2003，352，： | 1 |
| 10 | Biaxial warm forming behavior of aluminum Sheet alloys 显示文摘 | Li Daoming Amit K Ghosh | 2004 | Journal of Materials Processing Technology2004，145，： | 1 |
| 11 | Tensile deformation behavior of aluminum alloys at warm forming temperatures 显示文摘 | Li Daoming Amit Ghosh | 2003 | Materials Science and Engineering2003，352，： | 1 |
| 12 | Biaxial wall forming behavior of aluminum sheet alloys显示文摘 | LI Daoming Amit K G | 2004 | Journal of Materials Processing Technology2004，145，13： | 1 |
| 13 | Tensile deformation behavior of aluminum alloys at warm forming temperatures显示文摘 | Li Daoming Ghosh Amit | 2003 | Materials Science and Engineering2003，352，112： | 1 |
| 14 | Materials Science and Engineering A 显示文摘 | Li Daoming Ghosh A | 2003 | 352:2792003，352，： | 1 |
| 15 | Changes in soil properties under Eucalyptus relative to Pinus massoniana and natural broadleaved forests in South China显示文摘The ecological effects of eucalypt plantations(EPs) have garnered increasing attention.To understand their effect on soil quality at a landscape scale,and to determine whether soil quality parameters differ due to different stand types,we evaluated soil characteristics in twenty-one groups of EPs,Pinus massoniana Lamb.plantations(PMPs) and natural broadleaved forests(NBFs)across Guangdong Province,China.Both the physical characteristics of soil hydrology and the properties of soil nutrients in A and B horizons were determined.Results showed that,compared to NBFs,EPs and PMPs produced a shallower litter layer,reduced canopy density,higher soil bulk density,significantly lower total porosity,non-capillary porosity,total water volume,and hygroscopic water in the A horizon(P<0.05).Moreover,total N,available K,and soil organic carbon(SOC) in EPs and PMPs were significantly lower than in NBFs.EPs and PMPs did not differ significantly in N,P or K content,but PMPs had significantly lower SOC and boron in the A horizon than EPs.Low p H and poor capacity to buffer acidification generally occurred in all cover types.Both EPs and PMPs showed a decline in soil properties relative to NBFs,but EPs and PMPs exhibited no significant difference.These results indicate that actions are needed to ameliorate the potential negative effects on soil quality in forestry plantations. | Shuangshuang Chu Douglass F.Jacobs Joshua L.Sloan Li Xue Daoming Wu Shucai Zeng | 2018 | Journal of Forestry Research2018，29，5： | 1 |
| 16 | Tensile deformation behavior of aluminum alloys at warm forming temperature 显示文摘 | Daoming Li Amit Ghosh | 2003 | Materials Science and Engineering A2003，352，： | 1 |
| 17 | Biaxial warm forming behavior of aluminum sheet alloys 显示文摘 | Daoming Li Amit Ghosh | 2004 | Journal of Material Processing Technology2004，145，： | 1 |
| 18 | Tensile Deformation Behavior of Aluminum Alloys at Warm Forming Temperature显示文摘 | Daoming Li Amit Ghosh | 2003 | Materials Science and Engineering2003，352，： | 1 |
| 19 | Biaxial warm forming behavior of aluminum sheet alloys显示文摘 | Li Daoming Amit K Ghosh | | 0，，： | 1 |
| 20 | Biaxial warm forming behavior of aluminum sheet alloys显示文摘 | LI Daoming GHOSH Amit | 2004 | Journal of Materials Processing Technoiogym2004，，145： | 1 |
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