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| 1 | Assessment on alien herbicide-resistant gene flow among crucifers by sexual compatibility显示文摘Cross-pollination and high cross-compatibility are frequently found in many cruciferous plants, which im-plies that there might be potential risk of alien gene flow among crucifers. In this work, the alien glyphosate-resistant gene flow between GM (Genetically modified) rapeseed vari-ety Q3 (Brassica napus) and 6 varieties from 5 cruciferous species was assessed by sexual compatibility through aniline blue fluorescence observation, manual cross, mentor pollina-tion with the mixture pollen and DNA identification. The results indicated that the compatibility indices of two B. napus varieties, B. juncea var. multiceps Tsen et Lee and Chinese cabbage (B. chinensis) (as female) with Q3 (as male) were 16.15, 12.77, 2.345 and 0.85, respectively. F1 plants were examined by PCR and all of these samples were confirmed as positive. Crossing between Orychophragmus violaceus, Des-curainia sophia (as female) and Q3 (as male) expressed high cross-incompatibility. Fluorescence microscope observation indicated that growing of pollen tube terminated at the upper 1/3 part of the style of O. violaceus and on the stigma surface of D. sophia. The mentor plants were further analyzed by PCR and all were shown to be negative. Under the neighboring growth and natural pollination conditions, the rates of gene flow to two B. napus varieties were 2.3278% and 2.1487%, respectively, B. juncea var. multiceps Tsen et Lee and Chinese cabbage were 1.0157% and 0.9236%, re-spectively. No gene flow was observed in O. violaceus and D. sophia. Frequency of gene flow was highly correlated with sexual compatibility. Due to possible alien gene flow among crucifers, special consideration and care should be taken to grow GM rapeseed. | ZHAO Xiangxiang LU Weiping QI Cunkou PU Huiming XIA Qiuxia LU Dalei LIU Geshan WANG Youping | 2005 | Chinese Science Bulletin2005，50，15： | 6 |
| 2 | Application of moderate nitrogen levels alleviates yield loss and grain quality deterioration caused by post-silking heat stress in fresh waxy maize显示文摘High temperature(HT)during grain filling is one of the most important environmental factors limiting maize yield and grain quality.Nitrogen(N)fertilizer is essential for maintaining normal plant growth and defense against environmental stresses.The effects of three N rates and two temperature regimes on the grain yield and quality of fresh waxy maize were studied using the hybrids Suyunuo 5(SYN5)and Yunuo 7(YN7)as materials.N application rates were 1.5,4.5,and 7.5 g plant-1,representing low,moderate,and high N levels(LN,MN,and HN,respectively).Mean day/night temperatures during the grain filling of spring-and summer-sown plants were 27.6/21.0°C and 28.6/20.0°C for ambient temperature(AT)and 35/21.0°C and 35/20.0°C for HT,respectively.On average,HT reduced kernel number,weight,yield,and moisture content by 29.8%,17.9%,38.7%,and 3.3%,respectively.Kernel number,weight,yield,moisture,and starch contents were highest under MN among the three N rates under both temperature regimes.HT reduced grain starch content at all N levels.HT increased grain protein content,which gradually increased with N rate.Mean starch granule size under MN was larger(10.9μm)than that under LN and HN(both 10.4μm)at AT.However,the mean size of starch granules was higher under LN(11.7μm)and lower under MN(11.2μm)at HT.Iodine binding capacity(IBC)was lowest under MN and highest under HN among the three N levels under both temperature regimes.In general,IBC at all N rates was increased by HT.Peak viscosity(PV)was gradually reduced with increasing N rate at AT.In comparison with LN,PV was increased by MN and decreased by HN at HT.Retrogradation percentage gradually increased with N rate at AT,but was lowest under MN among the three N rates at HT.LN+AT and MN+HT produced grain with high pasting viscosity and low retrogradation tendency.MN application could alleviate the negative effects of HT on the grain yield and quality of fresh waxy maize. | Jue Wang Pengxiao Fu Weiping Lu Dalei Lu | 2020 | The Crop Journal2020，8，6： | 5 |
| 3 | Effects of shading after pollination on kernel filling and physicochemical quality traits of waxy maize显示文摘Understanding the effects of shading after pollination on kernel filling and physicochemical properties of waxy maize could improve kernel quality.Experiments were conducted to investigate the effects of shading(30% and 50% light deprivation,taken plants without shading as control) on kernel weight,size,and physicochemical properties during kernel development in 2013 and 2014 using two waxy maize varieties(Suyunuo 5 and Yunuo 7).Results indicated that shading reduced kernel filling rate and decreased kernel size and weight,and the influence was large under severe light deprivation conditions.The large reduction in kernel weight and volume of Suyunuo 5 in response to shading indicated that it was more sensitive to shading than Yunuo 7.Starch content was reduced and protein content was increased by shading,especially under severe shading after 22 days after pollination(DAP).The iodine binding capacity of Yunuo 7 was not affected by shading at fresh and maturity stages but was gradually decreased by shading at the newly formed stage,while the values for Suyunuo 5were decreased at 7 and 40 DAP only by moderate shading and were similar among three treatments at 22 DAP.Severe shading decreased crystallinity at all kernel development stages,whereas moderate shading decreased crystallinity at fresh stage and increased it at mature stage for Suyunuo 5.Crystallinity in Yunuo 7 was increased by shading at 7 DAP and decreased by shading at 40 DAP,whereas the value at 22 DAP was increased by moderate shading and reduced by severe shading,respectively.The average gelatinization temperatures at different stages were decreased by shading and showed no difference between two shading levels.The retrogradation percentage at 7 DAP for both varieties was increased by shading.The value at 22 DAP was increased by moderate shading for Suyunuo 5 and decreased by severe shading for Yunuo 7,respectively.The retrogradation percentage at 40 DAP was decreased by shading treatments for Suyunuo 5 and reduced only by moderate shading for Yunuo 7.Peak viscosity was decreased by shading at fresh stage for Yunuo 7 and at maturity for Suyunuo 5.In conclusion,shading after pollination inhibited kernel filling of waxy maize and reduced paste viscosity quality,but kernel retrograde quality,crystallinity and starch iodine binding capacity in response to shading were dependent on stage and variety. | Huan Yang Yanling Shi Renchao Xu Dalei Lu Weiping Lu | 2016 | The Crop Journal2016，4，3： | 4 |
| 4 | Gene flow from genetically modified herbicide-resistant rapeseed to cruciferous weeds显示文摘性相容性在之间遗传上修改了(美国通用汽车公司)glyphosate 抵抗的油菜籽变化 Q3 (Brassica 马来麝 L.) 和 5 棵饰有十字架的杂草通过荧光显微镜学的观察被学习并且在用手的授粉以后跨富饶。结果显示了那 Q3 (同样男性) 与 Thlaspi arvense L. 高度不兼容, Capsella bursa-pastoris (L.) 苜蓿, Cardamine hirsuta L。并且 Rorippa palustris (L.) Besser (作为女性) 。荧光显微镜的观察显示出那成长在耻辱表面上或在风格的上面的 1/3 部分终止的花粉试管。然而, B。juncea x Q3 是兼容的,并且相容性索引是 1.65。在附近的生长和自然授粉条件下面,基因的率从 Q3 流动到 T。arvense, C。bursa-pastoris, C。多毛并且 R。palustris 都是 0,当它为 B 是 0.86% 时。juncea。这些结果显示处于在美国通用汽车公司油菜籽之间的基因流动的率有差别,基因流动的饰有十字架的野杂草。 | ZHAO Xiangxiang XIA Oiuxia LU Dalei LU Weiping QI Cunkou PU Huiming LIU Geshan ZHAO Jian WANG Youping | 2006 | Progress in Natural Science:Materials International2006，16，9： | 3 |
| 5 | Cross-regulation of the Nanog and Cdx2 promoters显示文摘在哺乳动物的胚胎,内部房间团(ICM ) 的分离和 trophectoderm (TE ) 的第一种房间命运选择,,被抄写因素, Oct4 和 Cdx2 的互相对抗的效果调整 pluripotency 因素, Nanog,是必要的指定 epiblast。我们分析了 Nanog 和 Cdx2 的倡导者,并且发现了这二个抄写因素同样相互地被调整。用有有条件的 TE 区别的一根胚胎的干细胞线,我们证明 Nanog overexpression 压制 TE 标记的 upregulation,当时 Nanog 击倒的 upregulates TE 标记的表示。我们推进 Nanog 和 Cdx2 绑在并且镇压的表演对方的倡导者。而 Nanog 大美人在 ICM 导致可检测的 Cdx2 表示,我们不管多么不观察胚囊开发的公开混乱,显示 Nanog 起到在 ICM 和 TE 的分离的 Oct4 的一个谄媚的作用。 | Lingyi Chen Akiko Yabuuchi Sarah Eminli Ayumu Takeuchi Chi-Wei Lu Konrad Hochedlinger George Q Daley | 2009 | Cell Research2009，19，9： | 3 |
| 6 | 腔隙性梗死与皮质、皮质下梗死患者载脂蛋白E基因多态性分析显示文摘目的:研究腔隙性梗死(腔梗)与皮质、皮质下梗死(皮梗)患者的载脂蛋白E(ApoE)基因多态性分布的差异及与多种血管危险因素相关性的差异.方法:设腔梗组(81例)和皮梗组(87例),以调查表方式调查患者一般状况及血管危险因素;晨起空腹采血分离血清,测血糖、血脂,并以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测法测定患者ApoE基因型.结果:①2组基因分布:腔梗组ε3/4+ε4/4型频率(25.9%)明显高于皮梗组(11.5%);ε4频率(17.9%)高于皮梗组(9.8%),P均<0.05.ε3/4及ε4/4的携带者发生腔梗危险度高于皮梗,OR=2.77,95%CI 1.21~6.38,P<0.05.②腔梗组高血压患者多于皮梗组,高血压患者患腔梗,危险度大于皮梗,OR=2.02,95%CI 1.07~3.80,P<0.05,.腔梗组血浆总胆固醇水平高于皮梗组,P<0.05.结论:ε3/4和ε4/4携带者发生腔梗的危险度大于皮梗,这可能和ε4增高TC水平,加速脑小动脉粥样硬化进程有关. | 刘大建 聂鹏 LU Jie LU Dalei | 2005 | 郑州大学学报(医学版)2005，40，4： | 3 |
| 7 | Upping the ante: recent advances in direct reprogramming 显示文摘 | Muller LU Daley GQ Williams DA | 2009 | Mol Ther2009，17，6： | 1 |
| 8 | Delaying application time of slow-release fertilizer increases soil rhizosphere nitrogen content,root activity,and grain yield of spring maize显示文摘Application of slow-release fertilizer(SF)is a nutrient-management measure aimed at improving maize nutrient use and yield and saving labor cost.One-time application of SF at sowing usually results in nutrient deficiency during the post-silking stage,owing to the long growth period of spring maize.This study was conducted to investigate the effects on spring maize of SF application stage(zero,three-,and six-leaf stages,designated as SF0,SF3,and SF6,respectively)on grain yield,total soil rhizosphere nitrogen(N)content,and root activity,in comparison with the conventional fertilization mode(CF,application of compound fertilizer at sowing time,and topdressing urea at six-leaf and tasseling stages)at the same fertilization level as the control.Compared with no fertilization(F0)and CF,SF increased grain number and weight.The maize cultivars Suyu 30(SY30)and Jiangyu 877(JY877)produced the highest grain yield and net return under SF6 treatment over the three years.SF6 increased enzymatic activities including oxidoreductase,hydrolase,transferase,and lyase in rhizosphere soil at silking(R1)and milking stages(R3).SF6 increased the total N contents of rhizosphere soil by 7.1%at R1 and 9.2%R3 stages compared with SF0.The activities of antioxidant enzymes in roots were increased under SF6 treatments at R1and R3.The mean root activities of SF0,SF3,and SF6 increased by 7.1%,12.8%,and 20.5%compared with CF at R1 and by 8.8%,13.0%,and 23.5%at R3.Delaying the application time of SF could increase grain yield by increasing total N content of rhizosphere soil,delaying root senescence,and increasing root activity at the late reproductive stage.Applying SF at the six-leaf stage is recommended as an effective fertilization strategy for the sustainable production of spring maize in southern China. | Guanghao Li Pengxiao Fu Guigen Cheng Weiping Lu Dalei Lu | 2022 | The Crop Journal2022，10，6： | 1 |
| 9 | New Benzotrithiophene Derivative with a Broad Band Gap for High Performance Polymer Solar Cells 显示文摘 | ZHAO Xiaoli YANG Dalei LU Hongying | 2013 | Polymer Chemistry2013，4，1： | 1 |
| 10 | Effects of waterlogging after pollination on the physicochemical properties of starch from waxy maize显示文摘 | Lu Dalei Cai Xuemei Shi Yaxing | 2015 | Food Chemistry2015，179，4： | 1 |
| 11 | Synthesis and Characterization of Poly(ω-pentadecalactone) for Its Industrial-scale Production显示文摘 | CHANG Qing LI Li YANG Dalei ZHANG Mingyao TON-THAT Minh-Tan HU Wei LU Shuai | 2015 | Chemical Research in Chinese Universities2015，31，4： | 0 |
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