|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Endothelium-specific SIRT1 overexpression inhibits hyperglycemia-induced upregulation of vascular cell senescence显示文摘The rapidly increasing prevalence of diabetes mellitus worldwide is one of the most serious and challenging health problems in the 21st century.Mammalian sirtuin 1(SIRT1) has been shown to decrease high-glucose-induced endothelial cell senescence in vitro and prevent hyperglycemia-induced vascular dysfunction.However,a role for SIRT1 in prevention of hyperglycemia-induced vascular cell senescence in vivo remains unclear.We used endothelium-specific SIRT1 transgenic(SIRT1-Tg) mice and wild-type(WT) mice to construct a 40-week streptozotocin(STZ)-induced diabetic mouse model.In this mode,42.9% of wild-type(WT) mice and 38.5% of SIRT1-Tg mice were successfully established as diabetic.Forty weeks of hyperglycemia induced significant vascular cell senescence in aortas of mice,as indicated by upregulation of expression of senescence-associated markers including p53,p21 and plasminogen activator inhibitor-1(PAI-1).However,SIRT1-Tg diabetic mice displayed dramatically decreased expression of p53,p21 and PAI-1 compared with diabetic WT mice.Moreover,manganese superoxide dismutase expression(MnSOD) was significantly downregulated in the aortas of diabetic WT mice,but was preserved in diabetic SIRT1-Tg mice.Furthermore,expression of the oxidative stress adaptor p66Shc was significantly decreased in aortas of SIRT1-Tg diabetic mice compared with WT diabetic mice.Overall,these findings suggest that SIRT1-mediated inhibition of hyperglycemia-induced vascular cell senescence is mediated at least partly through the reduction of oxidative stress. | CHEN HouZao WAN YanZhen ZHOU Shuang LU YunBiao ZHANG ZhuQin ZHANG Ran CHEN Feng HAO DeLong ZHAO Xiang GUO ZhiChen LIU DePei LIANG ChihChuan | 2012 | Science China(Life Sciences)2012，55，6： | 14 |
| 2 | Bond-selective transient phase imaging via sensing of the infrared photothermal effect显示文摘Phase-contrast microscopy converts the phase shift of light passing through a transparent specimen,e.g.,a biological cell,into brightness variations in an image.This ability to observe structures without destructive fixation or staining has been widely utilized for applications in materials and life sciences.Despite these advantages,phase-contrast microscopy lacks the ability to reveal molecular information.To address this gap,we developed a bond-selective transient phase(BSTP)imaging technique that excites molecular vibrations by infrared light,resulting in a transient change in phase shift that can be detected by a diffraction phase microscope.By developing a time-gated pump-probe camera system,we demonstrate BSTP imaging of live cells at a 50 Hz frame rate with high spectral fidelity,sub-microsecond temporal resolution,and sub-micron spatial resolution.Our approach paves a new way for spectroscopic imaging investigation in biology and materials science. | Delong Zhang Lu Lan Yeran Bai Hassaan Majeed Mikhail E.Kandel Gabriel Popescu Ji-Xin Cheng | 2019 | Light(Science & Applications)2019，8，1： | 3 |
| 3 | Genome-wide identification and expression analysis of DNA demethylase family in cotton显示文摘Background:DNA methylation is an important epigenetic factor that maintains and regulates gene expression.The mode and level of DNA methylation depend on the roles of DNA methyltransferase and demethylase,while DNA demethylase plays a key role in the process of DNA demethylation.The results showed that the plant’s DNA demethylase all contained conserved DNA glycosidase domain.This study identified the cotton DNA demethylase gene family and analyzed it using bioinformatics methods to lay the foundation for further study of cotton demethylase gene function.Results:This study used genomic information from diploid Gossypium raimondii JGI(D),Gossypium arboreum L.CRI(A),Gossypium hirsutum L.JGI(AD1) and Gossypium barbadebse L NAU(AD2) to Arabidopsis thaliana.Using DNA demethylase genes sequence of Arabidopsis as reference,25 DNA demethylase genes were identified in cotton by BLAST analysis.There are 4 genes in the genome D,5 genes in the genome A,10 genes in the genome AD1,and 6 genes in the genome AD2.The gene structure and evolution were analyzed by bioinformatics,and the expression patterns of DNA demethylase gene family in Gossypium hirsutum L were analyzed.From the phylogenetic tree analysis,the DNA demethylase gene family of cotton can be divided into four subfamilies:REPRESSOR of SILENCING 1(ROS1),DEMETER(DME),DEMETER-LIKE 2(DML2),and DEMETER-LIKE3(DML3).The sequence similarity of DNA demethylase genes in the same species was higher,and the genetic relationship was also relatively close.Analysis of the gene structure revealed that the DNA demethylase gene family members of the four subfamilies varied greatly.Among them,the number of introns of ROS1 and DME subfamily was larger,and the gene structure was more complex.For the analysis of the conserved domain,it was known that the DNA demethylase family gene member has an endonuclease Ⅲ(END03 c) domain.Conclusion:The genes of the DNA demethylase family are distributed differently in different cotton species,and the gene structure is very different.High expression of ROS1 genes in cotton were under abiotic stress.The expression levels of ROS1 genes were higher during the formation of cotton ovule.The transcription levels of ROS1 family genes were higher during cotton fiber development. | YANG Xiaomin LU Xuke CHEN Xiugui WANG Delong WANG Junjuan WANG Shuai GUO Lixue CHEN Chao WANG Xiaoge WANG Xinlei YE Wuwei | 2019 | Journal of Cotton Research2019，2，3： | 3 |
| 4 | Immobilization of biomacromolecules on poly-L-lactide surface via a layer-by-layer method for the improving of its cytocompatibility to bone marrow stromal cells显示文摘Hyaluronic acid (HA) and chitosan (CS) were immobilized on the surface of poly-L-lactide (PLLA) by the following procedure: Firstly, PLLA was aminolyzed with 1, 6-hexanediamine, and part of the PLLA surface ester groups were converted to free amino groups. Then negatively charged hyaluronic acid and positively charged chitosan were deposited onto the surface of aminolyzed PLLA film in a layer-by-layer assembly manner. The effect of the layer-by- layer deposition was evaluated by ATR-FTIR spectroscopy, Raman spectroscopy and static contact angle measurements. The cytocompatibility of PLLA sample to bone marrow stromal cells (BMSCs) was improved after modification with chitosan and HA. The cell attachment, activity, and prolif- eration on CS/HA modified PLLA films were enhanced comparing with the control. The cells cultured on the modi- fied PLLA samples excreted abundant cytoplasm and can differentiate to vascular smooth muscle (SM)-like (SM-like) cells. A macroporous three-dimensional PLLA scaffold was prepared by integrating both the technique of freeze-drying and particle leaching. Layer-by-layer modification by HA/CS and cell culture was also applied on this scaffold. The scaf- fold cultured with BMSCs for 2 weeks has been tested suc- cessfully in vivo as a patch for repairing the artificial incision on canine pulmonary artery. | LU Delong MENG Sheng ZHONG Wei DU Qiang-guo GONG Li LIU Jinfen Dusan Bakos | 2005 | Chinese Science Bulletin2005，50，24： | 2 |
| 5 | Purification and characterization of a novel marine Arthrobacter Oxydans KQ11 dextranase显示文摘 | Delong Wang Mingsheng Lu Shujun Wang Yuliang Jiao Weijuan Li Qiang Zhu Zhaopu Liu | 2014 | Carbohydrate Polymers2014，，： | 1 |
| 6 | Overexpression of SLC7A7 predicts poor progression-free and overall survival in patients with glioblastoma显示文摘 | Songhua Fan Delong Meng Tao Xu Yuanyuan Chen Jingkun Wang Xiaoying Li Hongyan Chen Daru Lu Juxiang Chen Qing Lan | 2013 | Medical Oncology2013，，1： | 1 |
| 7 | Study on Eogene Basalts: Implication of the Deep Process over the Bohaiwan Basin Evolution显示文摘StudyonEogeneBasalts:ImplicationoftheDeepProcesovertheBohaiwanBasinEvolution*YeDelongLuFengxiangZhuQinwenZhengJianpingFaculty... | Ye Delong Lu Fengxiang Zhu Qinwen Zheng JianpingFaculty of Earth Sciences, China University of Geosciences, Wuhan 430074 | 1997 | Journal of Earth Science1997，16，1： | 1 |
| 8 | Improv- ing stability of a novel dextran-degrading enzyme from marine Arthrobacter oxydans KQ11 显示文摘 | WANG Delong LU Mingsheng WANG Xiaobei | 2014 | Carbohydrate polymers2014，103，4： | 1 |
| 9 | Characters and structures of the nucleobase-ascorbate transporters(NAT)family genes in Gossypium hirsutum and their roles in responding to salt and drought stresses显示文摘Background: Nucleobase-ascorbate transporters(NAT), synonymously called nucleobase-cation symporter 2(NCS2) proteins, were earlier reported to be involved in plant growth, development and resistance to stress. Previous studies concluded that s a polymorphic SNP associated with NAT12 was significant di erent between salt-tolerant and salt-sensitive materials of upland cotton. In current study, a comprehensive analysis of NAT family genes was conducted for the first time in cotton.Results: In this study, we discovered 32, 32, 18, and 16 NAT genes in Gossypium hirsutum, G. barbadense, G. raimondii and G. arboreum, respectively, which were classified into four groups(groups I–IV) based on the multiple sequence analysis. These GhNAT genes were unevenly distributed on At and Dt sub-genome in G. hirsutum. Most GhNAT members in the same group had similar gene structure characteristics and motif composition. The collinearity analysis revealed segmental duplication as well as tandem duplication contributing to the expansion of the GhNATs. The analysis of cis-acting regulatory elements of GhNATs showed that the function of GhNAT genes in cotton might be related to plant hormone and stress response. Under di erent conditions, the expression levels further suggested the GhNAT family genes were associated with plant response to various abiotic stresses. GhNAT12 was detected in the plasma membrane. And it was validated that the GhNAT12 gene played an important role in regulating cotton resistance to salt and drought stress through the virus-induced gene silencing(VIGS) analysis.Conclusions: A comprehensive analysis of NAT gene family was performed in cotton, including phylogenetic analysis, chromosomal location, collinearity analysis, motifs, gene structure and so on. Our results will further broaden the insight into the evolution and potential functions of NAT genes in cotton. Current findings could make significant contribution towards screening more candidate genes related to biotic and abiotic resistance for the improvement in cotton. | GUO Lixue ZHAO Lanjie LU Xuke CHEN Xiugui WANG Shuai WANG Junjuan WANG Delong YIN Zujun CHEN Chao FAN Yapeng ZHANG Yuexin YE Wuwei | 2022 | Journal of Cotton Research2022，5，2： | 0 |
| 10 | Functional structure analysis and genome‑wide identification of CNX gene family in cotton显示文摘Background:Under abiotic stress conditions,cotton growth is inhibited and yield losses are severe.Identification of calnexin family members and function analysis under abiotic stress laid the foundation for the screening of stressrelated candidate genes.Results:A total of 60 CNX family members have been identified in Gossypium hirsutum,G.barbadense,G.arboreum,and G.raimondii,and they were divided into two categories:CNX and CRT genes.Through the construction of a phylogenetic tree,they were subdivided into three classes.Further analysis of chromosome localization,conserved promoters,gene structure and selection under pressure showed that the family members were highly conserved in the evolution process.Analysis of cis-acting elements in the promoter regions showed that CNX family genes contain regulatory elements for growth and development,anaerobic,drought,defense and stress response,and plant hormones.Using RNA-seq data to study the expression pattern of GhCNX genes under cold,hot,salt stress and Polyethylene glycol,it was observed that the gene expression levels changed by different degrees under different stress conditions,indicating that GhCNX members were involved in the regulation of multiple biological stresses.Conclusion:This study provides an insight into the members of cotton CNX genes.The results of this study suggested that CNX family members play a role in defense against adversity and provide a foundation for the discovery of stress-related genes. | XU Nan ZHANG Hong ZHANG Yuexin FAN Yapeng WANG Jing MALIK Waqar Afzal RUI Cun HAN Mingge LU Xuke CHEN Xiugui WANG Junjuan WANG Delong WANG Shuai CHEN Chao GUO Lixue ZHAO Lanjie YE Wuwei | 2022 | Journal of Cotton Research2022，5，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员