|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface显示文摘A new cell membrane preparation, silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the adsorption. The surface characteristics of the silica CCM have been tested by a scanning electron microscope and surface energy spectrometer. Comparison has been made of the changeable rule of enzymatic activity of rabbit red cell membrane in three states: the silica CCM, the suspension and the precipitation cell membranes, with temperature and time. The result showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes. The interactions between calcium antagonists and rabbit red and cardiac muscle cell membranes, and the stereoscopic interactions between dihydropridines and rabbit cerebellar cell membrane have been investigated by the chromatographic system of the silica CCM as a stationary phase. From the foregoing results, the chromatographic parameters obtained from the system would | Langchong He Guangde Yang Xindu Geng | 1999 | Chinese Science Bulletin1999，44，9： | 58 |
| 2 | Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR_4 receptor component from Atractylodes macrocephala Koidz显示文摘A model of white blood cell membrane chromatography (WB-CMC) was established to screen active component from Atractylodes macrocephala Koidz. The component can antagonize Toll-like receptor 4 (TLR4) and inhibit inflammatory reaction. In the model of WB-CMC, cell membrane stationary phase (CMSP) was prepared by immobilizing the rabbit white blood cell membrane (WBCM) onto the surface of silica carrier and taxinol was used as a model molecule. The active component which can act on WBCM and its receptor (such as TLR4) as an effective target in A. macrocephala was determined by using a replacement experiment. The anti-inflammatory effects of the active component were tested by using pharmacological methods in vivo. The results indicated that the re-tention characteristics of atractylenolide I as active component was similar to that of taxinol in the model of WB-CMC. And so, atractylenolide I acted on the WBCM and TLR4 and its anti-inflammatory activity was related with antagonizing TLR4. Therefore, the interaction between the active component and WBCM and its receptor can be simulated by the model of WB-CMC in vitro. This model can be used to screen active components and to study effective characteristics for acting on definite targets. | LI Cuiqin HE Langchong | 2006 | Science China(Life Sciences)2006，49，2： | 18 |
| 3 | Establishment of the model of vascular endothelial cell membrane chromatography and its preliminary application显示文摘A model of vascular endothelial cell membrane chromatography was established by using an ECV304 cell membrane stationary phase (ECV304 CMSP) prepared by immobilizing the ECV304 cell membrane onto the surface of silica carrier. The surface and chromatographic characteristics of ECV304 CMSP were studied. The active component from Caulophyllum robustum was screened by using the model of vascular endothelial cell membrane chromatography. The interaction between the active component and membrane receptor was determined by using a replace experiments. The effect of the active component was tested by using tube formation of ECV304 cell. The results indicated that the model of ECV304 cell membrane chromatograph (ECV304 CMC) can stimulate the interaction between drug and receptor in vitro and the retention characteristics of taspine as active component was similar to that of model molecule in the model of ECV304 CMC. And therefore, taspine acted on VEGFR2 and inhibited the tube formation of ECV304 cell induced by VEGF. This model can be used to screen definite active component as a screening model. | LI YiPing HE LangChong | 2007 | Chinese Science Bulletin2007，52，7： | 6 |
| 4 | EGFR/cell membrane chromatography-online-high performance liquid chromatography/mass spectrometry method for screening EGFR antagonists from Radix Angelicae Pubescentis显示文摘The intracellular kinase domains of the epidermal growth factor receptor(EGFR) in some tumor cells are significant targets for drug discovery.We have developed a new EGFR cell membrane chromatography(EGFR/CMC)-online-high performance liquid chromatography/mass spectrometry(HPLC/MS) method for screening anti-EGFR antagonists from medicinal herbs such as Radix Angelicae Pubescentis.In this study,the HEK293 EGFR cells with high expression of EGFR were used to prepare cell membrane stationary phase(CMSP) in the EGFR/CMC model.The retention fractions on the EGFR/CMC model were directly analyzed by combining a 10 port columns switcher with a HPLC/MS system online.As a result,osthole from Radix Angelicae Pubescentis was found to be the active component acting on EGFR like dasatinib as the control drug.There was a good relationship between their inhibiting effects on EGFR secretion and HEK293 EGFR cell growth in vitro.This new EGFR/CMC-online-HPLC/MS method can be applied for screening anti-EGFR antagonists from TCMs,for instance,Radix Angelicae Pubescentis.It will be a useful method for drug discovery with natural medicinal herbs as a leading compound resource. | SiCen Wang Meng Sun YanMin Zhang Jie Zhang LangChong He | 2010 | Science China Chemistry2010，53，11： | 5 |
| 5 | Overview of the detection methods for equilibrium dissociation constant KD of drug-receptor interaction显示文摘Drug-receptor interaction plays an important role in a series of biological effects, such as cell proliferation, immune response, tumor metastasis, and drug delivery. Therefore, the research on drug-receptor interaction is growing rapidly. The equilibrium dissociation constant(K_D) is the basic parameter to evaluate the binding property of the drug-receptor. Thus, a variety of analytical methods have been established to determine the KDvalues, including radioligand binding assay, surface plasmon resonance method, fluorescence energy resonance transfer method, affinity chromatography, and isothermal titration calorimetry. With the invention and innovation of new technology and analysis method, there is a deep exploration and comprehension about drug-receptor interaction. This review discusses the different methods of determining the K_D values, and analyzes the applicability and the characteristic of each analytical method. Conclusively, the aim is to provide the guidance for researchers to utilize the most appropriate analytical tool to determine the K_D values. | Weina Ma Liu Yang Langchong He | 2018 | Journal of Pharmaceutical Analysis2018，8，3： | 3 |
| 6 | Development of the model of pancreatic β-cell membrane chromatography and its chromatographic characteristics显示文摘A new model of pancreatic β-cell membrane chromatography has been established by using a β-cell membrane stationary phase (β-CMSP) prepared by immobi-lizing the β-cell membrane onto the surface of silica carrier. The protein level and K+, Na+-ATP enzymatic activity of the β-CMSP were detected respectively. The surface characteris-tics of the β-CMSP were tested by using the scanning elec-tron microscope and surface energy spectrometer. In this model, the column (10 mm × 2 mm, I.D.) packed with β-CMSP, 25 mmo1/L ammonium sulfate buffer solution (pH 7.4) as mobile phase with the flow rate of 0.2 mL/min at 37 ± 0.5℃ were used in the following studies. The retention char-acteristics of the sulfonylureas (gliquidone, glibenclamide, gliclazide and glipizide) were investigated under the chro-matographic conditions above. The affinities of the sulfony-lureas on β-cell membrane and receptor will be expressed by using the logk′ values (the logarithm of capacity factor of a solute) in the model. The correlation of the affinity with the pharmacological effect of the sulfonylureas was analyzed also. | YANG Guangde HE Langchong BIAN Xiaoli ZHAO Liang | 2005 | Chinese Science Bulletin2005，50，21： | 2 |
| 7 | Uniformly sized molecularly imprinted polymers for on-line concentration, purification, and measurement of nimodipine in plasma显示文摘 | Fu Qiang He Langchong Zhang Qianqian | 2009 | Journal of Applied Polymer Science2009，111，： | 1 |
| 8 | Taspine Isolated from Radix et Rhizoma Leonticis Inhibits Proliferation and Migration of Endothelial Cells as well as Chicken Chorioallantoic Membrane Neovascularisation显示文摘 | Zhang Yanmin He Langchong Meng Liang | 2008 | Vasc Pharmacol2008，48，23： | 1 |
| 9 | Suppression of Tumor-induced Angiogenesis by Taspine Isolated from Radix et Rhizoma Leonticis and Its Mechanism of Action in vitro显示文摘 | Zhang Yanmin He Langchong Meng Liang | 2008 | Cancer Lett2008，262，1： | 1 |
| 10 | Taspine Isolated from Radix et Rhizoma Leonticis Inhibits Growth of Human Umbilical Vein Endothelial Cell ( HUVEC ) by Inducing Its Apoptosis 显示文摘 | Zhang Yanmin He Langchong Zhou Yali | 2008 | Phytomedicine2008，15，12： | 1 |
| 11 | Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics显示文摘 | Langchong He Sicen Wang Xindu Geng | 2001 | Chromatographia (-)2001，，1： | 1 |
| 12 | Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics显示文摘 | Langchong He Sicen Wang Xindu Geng | 2001 | Chromatographia (-)2001，，1： | 1 |
| 13 | Inhibitory effect of atractylenolide I on the angiogenesis in chronic inflammation in vivo and in vitro显示文摘 | Wang Changhe Duan Haijie He Langchong | 2009 | Eur J Phamlacol2009，612，2： | 1 |
| 14 | Investigation of calcium antagonist–L-type calcium channel interactions by a vascular smooth muscle cell membrane chromatography method显示文摘 | Hui Du Jianyu He Sicen Wang Langchong He | 2010 | Analytical and Bioanalytical Chemistry2010，，5： | 1 |
| 15 | Comparison of alpha1-adrenergic receptor cell-membrane stationary phases prepared from expressed cell line and from rabbit hepatocytes显示文摘 | Yu Wang Bingxiang Yuan Xiuling Deng Langchong He Sicen Wang Youyi Zhang Qide Han | 2006 | Analytical and Bioanalytical Chemistry (-)2006，，7： | 1 |
| 16 | Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics显示文摘 | Langchong He Sicen Wang Xindu Geng | 2001 | Chromatographia (-)2001，，1： | 1 |
| 17 | Coating and Fusing Cell Membrane Onto a Silica Surface and Their Chromatographic Characteristics显示文摘 | He Langchong Wang Sicen Geng Xindu | 2001 | Chromatographia2001，54，： | 1 |
| 18 | Coating and Fusing Cell Membrane Onto a Silica Surface and Their Chromatographic Characteristics 显示文摘 | Sicen Wang Xindu Geng | 2001 | Chromatographia2001，54，： | 1 |
| 19 | Chromatographic Features and Molecular Recognition Mechanism of a Strychnine Monolithic Molecularly Imprinted Polymer显示文摘 | Jing Zhang Langchong He Qiang Fu | 2005 | Chromatographia (-)2005，，5： | 1 |
| 20 | Cell Membrane Chromatography Correlated with Functional Assay for Ligand–β-Adrenergic Receptor Affinities显示文摘 | Aiguo Zeng Bingxiang Yuan Fan Zhu Limei Zhao Langchong He Guangde Yang | 2009 | Chromatographia (-)2009，，11： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员