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2篇 您的检索式：作者名="Laura DeBusk"

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		题名	作者	年代	出处	被引量
	1	Expansion of myeloid immune suppressor Gr+CD11b+ cells in tumor-bearing host directly promotes tumor angiogenesis显示文摘	Li Yang Laura M. DeBusk Koari Fukuda Barbara Fingleton Brenda Green-Jarvis Yu Shyr Lynn M. Matrisian David P. Carbone P.Charles Lin	2004	Cancer Cell2004，，：	1
	2	Spontaneous immortalization of human dermal microvascular endothelial cells显示文摘AIM:To establish and characterize a spontaneously immortalized human dermal microvascular endothelial cell line,iHDME1.METHODS:We developed a spontaneous immortalization method.This approach is based on the application of optimized culture media and culture conditions without addition of any exogenous oncogenes or carcinogens.Using this approach,we have successfully established a microvascular endothelial cell line,iHDME1,from primary human dermal microvascular endothelial cells.iHDME1 cells have been maintained in culture dishes for more than 50 passages over a period of 6 mo.Using a GFP expressing retrovirus,we generated a GFP-stable cell line(iHDME1-GFP).RESULTS:iHDME1 retain endothelial morphology and uniformly express endothelial markers such as VEGF receptor 2 and VE-cadherin but not α-smooth muscle actin(α-SM-actin) and cytokeratin 18,markers for smooth muscle cells and epithelial cells respectively.These cells retain endothelial properties,migrate in response to VEGF stimulation and form 3-D vascular structures in Matrigel,similar to the parental cells.There is no signif icant difference in cell cycle prof ile between the parental cells and iHDME1 cells.Further analysis indicates enhanced stemness in iHDME1 cells compared to parental cells.iHDME1 cells display elevated expression of CD133 and hTERT.CONCLUSION:iHDME1 cells will be a valuable source for studying angiogenesis.	Laura DeBusk Suzanne Fernandez Douglas W Strand Simon W Hayward P Charles Lin	2010	World Journal of Stem Cells2010，2，5：	1

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