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| 1 | Preparation and Properties of Zirconium Hydrideon the surface of MCM-41 Mesoporous Molecular Sieves显示文摘Zirconium monohydride bonded to the framework oxygen of MCM-41 surface was prepared by the reaction of tetraneopentyl zirconium with MCM-41 surface hydroxyl groups, followed by the hydrogenolysis of the resulted product. The surface hydride was characterized by using infrared spectroscopy, solid-state NMR, elemental analysis, gas-phase chromatography and chemical probing reaction. It was shown that this surface species is stable below 150 'C and can catalytically crack alkanes into methane and ethane at 100 . | WANG Xu-xu1* * , FU Xian-zhi1, LEFEBVRE Frederic2 and BASSET Jean-Marie2(1. Research Institute of Photocatalysis, Fuzhou University, Fuzhou 350002, P. R. China 2. Laboratoire de Chimie Organometallique de Surface, UMR CNRS-CPE 9986,43,Bd dull Novembre 1 | 2001 | Chemical Research in Chinese Universities2001，17，4： | 4 |
| 2 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 3 | Reproducfive failure in sows following experimental infection with a Belgian EMCV isolate显示文摘 | KOENEN F DE CLERCQ K LEFEBVRE J | 1994 | Vet Microbio11994，39，12： | 1 |
| 4 | Potent inhibition of the master chondrogenic factor Sox9 gene by inteleukin-1 and t umor necrosis factor alpha显示文摘 | Murakami S Lefebvre V de Crombrugge B | 2000 | Biol Chem2000，275，5： | 1 |
| 5 | Reproductive failure in sows following experimental infection with a Belgian EMCV isolate 显示文摘 | KOENEN F DE C K LEFEBVRE J | 1994 | Vet Microbiol1994，39，： | 1 |
| 6 | A new long form of SOX5 (L -SOXS), SOX6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type Ⅱ collagen gene显示文摘 | Lefebvre V Li P de Crombrugghe B | 1998 | EMBO J1998，17，： | 1 |
| 7 | Reproductive failure in sow s following experimental infection with a Belgian EMCV isolate 显示文摘 | KOENEN F de CLERCQ K LEFEBVRE J | 1994 | Vet Micro1994，39，： | 1 |
| 8 | Smoking impairs bradykinin-stimulated t-PA release显示文摘 | Pretorius M Rosenbaum DA Lefebvre J Vauqhan DE Blown HJ | 2002 | Hypertension2002，39，3： | 1 |
| 9 | T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3显示文摘 | Chakir H Wang H Lefebvre DE | 2003 | J Immunol Methods2003，278，： | 1 |
| 10 | 5-HT4 receptor agonists:similar but not the same显示文摘 | De Maeyer JH Lefebvre RA Schuurkes JA | | 0，，02： | 1 |
| 11 | T-bet/GATA3 ratio as a measure of the TH1/TH2 cymkine profile in mixed cell populations:predominant role of GATA3显示文摘 | Chakir H Wang H Lefebvre DE | 2003 | J Immunol Methods2003，278，： | 1 |
| 12 | Regulatory mechanisms in the pathways of cartilage and bone formation显示文摘 | de Crombrugghe B Lefebvre V Nakashima K | 2001 | Curt Opin Cell Biol2001，13，6： | 1 |
| 13 | Kalman filters for non-linear systems: a comparison of performance显示文摘 | Tine Lefebvre Herman Bruyninckx Joris De Schutter | 2004 | International Journal of Control2004，，7： | 1 |
| 14 | Reproductive failure in sows following experimental infection with a Belgian EMCV isolate 显示文摘 | Koenen F De Clercq K Lefebvre J | 1994 | Vet Mierob ol1994，39，： | 1 |
| 15 | L SoxS, Sox6 and SOX9 control essential steps of the chondrocyte differentiation pathway 显示文摘 | Lefebvre V Behringer RR de Crombrugghe B | 2001 | Osteoarthritis Cartilage2001，9，： | 1 |
| 16 | Pharmacologic treatment of inner ear:from basic science to the patient显示文摘 | Lefebvre PP Staecker H Van de Water T | 2002 | Acta Otorhinolaryngol Belg2002，56，1： | 1 |
| 17 | Selective desensitization of the 5-HT4 receptor-mediated response in pig atrium but not in stomach 显示文摘 | De Maeyer J Schuurkes J Lefebvre R | 2009 | Br J Pharmacol2009，156，2： | 1 |
| 18 | T-bet/GATA-3 ratio as a measure of the Thl/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3 显示文摘 | Chakir H Wang H Lefebvre DE | 2003 | J Immunol Methods2003，278，12： | 1 |
| 19 | Potent inhibition of the master chondrogenic factor Sox9 gene by inteleukin-1 and tumor necrosis factor-alpha 显示文摘 | Murakami S Lefebvre V de Crombrugge B | 2000 | Biol Chem2000，275，5： | 1 |
| 20 | T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell population:predominant role of GATA-3显示文摘 | Chakir H Wang H Lefebvre DE | 2003 | J Immunol Medthods2003，278，： | 1 |
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