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| 1 | Interaction between Bms1 and Rcl1,two ribosome biogenesis factors,is evolutionally conserved in zebrafish and human显示文摘Ribosomes are large RNA and protein complexes that function as the machinery for translation protein synthesis(Boisvert et al.,2007;Ben-Shem et al.,2011;Henras et al.,2015;Khatter et al.,2015;McCann et al.,2015).The eukaryotic ribosome is composed of two subunits,the 60S large subunit(LSU)and the 40S small subunit(SSU),which collectively comprise of four different ribosomal RNA(rRNA)species and more than 70 proteins(Ben-Shem et al.,2011;Henras et al.,2015;Khatter et al.,2015).The LSU | Yong Wang Qinfang Zhu Ling Huang Yanqing Zhu Jun Chen Jinrong Peng Li Jan Lo | 2016 | Journal of Genetics and Genomics2016，43，7： | 2 |
| 2 | Hepatocytes in a normal adult liver are derived solely from the embryonic hepatocytes显示文摘In vertebrates,body weight increases many folds as a consequence of body growth from the childhood to adulthood(e.g.,~20 folds for a mouse).Considering the fact that the liver-to-body weight ratio(LBR)stays relatively constant within species(Weglarz and Sandgren,2000;Kan et al.,2009),the cell number in a liver must therefore keep increasing along with the | Ce Gao Zhihui Zhu Yuqi Gao Li Jan Lo Jun Chen Lingfei Luo Jinrong Peng | 2018 | Journal of Genetics and Genomics2018，45，3： | 2 |
| 3 | Zebrafish hhex-null mutant develops an intrahepatic intestinal tube due to de-repression of cdx1b and pdx1显示文摘The hepatopancreatic duct (HPD) system links the liver and pancreas to the intestinal tube and is composed of the extrahepatic biliary duct, gallbladder, and pancreatic duct. Haematopoietically expressed-homeobox (Hhex) protein plays an essential role in the establishment of HPD;however, the molecular mechanism remains elusive. Here, we show that zebrafish hhex-null mutants fail to develop the HPD system characterized by lacking the biliary marker Annexin A4 and the HPD marker sox9b. The hepatobiliary duct part of the mutant HPD system is replaced by an intrahepatic intestinal tube characterized by expressing the intestinal marker fatty acid-binding protein 2a (fabp2a). Cell lineage analysis showed that this intrahepatic intestinal tube is not originated from hepatocytes or cholangiocytes. Further analysis revealed that cdx1b and pdx1 are expressed ectopically in the intrahepatic intestinal tube and knockdown of cdx1b and pdx1 could restore the expression of sox9b in the mutant. Chromatin-immunoprecipitation analysis showed that Hhex binds to the promoters of pdx1 and cdx1b genes to repress their expression. We therefore propose that Hhex, Cdx1b, Pdx1, and Sox9b form a genetic network governing the patterning and morphogenesis of the HPD and digestive tract systems in zebrafish. | Ce Gao Weidong Huang Yuqi Gao Li Jan Lo Lingfei Luo Honghui Huang Jun Chen Jinrong Peng | 2019 | Journal of Molecular Cell Biology2019，11，6： | 2 |
| 4 | Loss-of-function of zebrafish cdt1 causes retarded body growth and underdeveloped gonads resembling human Meier-Gorlin syndrome显示文摘The cell cycle consists of four distinct phases:GO/Gl,S(DNA synthesis),G2,and M(mitosis).The Gl to S transition is typified by an accumulation of 4',6-diamidino-2-phenylindole(DAPI)signal that indicates the rapid DNA synthesis initiated at special sites on DNA,generally called the Ori(origin of replication)(Alfa et al.,1989). | Yinan HE Yong WANG Yanqing ZHU Li Jan LO | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，11： | 0 |
| 5 | Nucleolar GTPase Bms1 displaces Ttf1 from RFB-sites to balance progression of rDNA transcription and replication显示文摘18S,5.8S,and 28S ribosomal RNAs(rRNAs)are cotranscribed as a pre-ribosomal RNA(pre-rRNA)from the rDNA by RNA polymerase I whose activity is vigorous during the S-phase,leading to a conflict with rDNA replication.This conflict is resolved partly by replication-fork-barrier(RFB)-sites sequences located downstream of the rDNA and RFB-binding proteins such as Ttf1.However,how Ttf1 is displaced from RFB-sites to allow replication fork progression remains elusive.Here,we reported that loss-of-function of Bms1l,a nucleolar GTPase,upregulates rDNA transcription,causes replication-fork stall,and arrests cell cycle at the S-to-G2 transition;however,the G1-to-S transition is constitutively active characterized by persisting DNA synthesis.Concomitantly,ubf,tif-IA,and taf1b marking rDNA transcription,Chk2,Rad51,and p53 marking DNA-damage response,and Rpa2,PCNA,Fen1,and Ttf1 marking replication fork stall are all highly elevated in bms1l mutants.We found that Bms1 interacts with Ttf1 in addition to Rc1l.Finally,we identified RFB-sites for zebrafish Ttf1 through chromatin immunoprecipitation sequencing and showed that Bms1 disassociates the Ttf1–RFB complex with its GTPase activity.We propose that Bms1 functions to balance rDNA transcription and replication at the S-phase through interaction with Rcl1 and Ttf1,respectively.TTF1 and Bms1 together might impose an S-phase checkpoint at the rDNA loci. | Yanqing Zhu Yong Wang Boxiang Tao Jinhua Han Hong Chen Qinfang Zhu Ling Huang Yinan He Jian Hong Yunqin Li Jun Chen Jun Huang Li Jan Lo Jinrong Peng | 2021 | Journal of Molecular Cell Biology2021，13，12： | 0 |
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