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| 1 | Prognostic assessment of apoptotic gene polymorphisms in non-small cell lung cancer in Chinese显示文摘Apoptosis plays a key role in inhibiting tumor growth, progression and resistance to anti-tumor therapy. We hypothesized that genetic variants in apoptotic genes may affect the prognosis of lung cancer. To test this hypothesis, we selected 38 potentially functional single nucleotide polymorphisms (SNPs) from 12 genes (BAX, BCL2, BID, CASP3, CASP6, CASP7, CASP8, CASP9, CASP10, FAS, FASLG and MCL1) involved in apoptosis to assess their prognostic significance in lung cancer in a Chinese case cohort with 568 non-small cell lung cancer (NSCLC) patients. Thirty-five SNPs passing quality control underwent association analyses, 11 of which were shown to be significantly associated with NSCLC survival (P<0.05). After Cox stepwise regression analyses, 3 SNPs were independently associated with the outcome of NSCLC (BID rs8190315: P=0.003; CASP9 rs4645981: P=0.007 and FAS rs1800682: P=0.016). A favorable survival of NSCLC was significantly associated with the genotypes of BID rs8190315 AG/GG (adjusted HR=0.65, 95% CI: 0.49-0.88), CASP9 rs4645981 AA (HR=0.22, 95% CI: 0.07-0.69) and FAS rs1800682 GG (adjusted HR=0.67, 95% CI: 0.46-0.97). Time-dependent receptor operation curve (ROC) analysis revealed that the area under curve (AUC) at year 5 was significantly increased from 0.762 to 0.819 after adding the risk score of these 3 SNPs to the clinical risk score. The remaining 32 SNPs were not significantly associated with NSCLC prognosis after adjustment for these 3 SNPs. These findings indicate that BID rs8190315, CASP9 rs4645981 and FAS rs1800682 polymorphisms in the apoptotic pathway may be involved in the prognosis of NSCLC in the Chinese population. | Songyu Cao Cheng Wang Xinen Huang Juncheng Dai Lingmin Hu Yao Liu Jiaping Chen Hongxia Ma Guangfu Jin Zhibin Hu Lin Xu Hongbing Shen | 2013 | The Journal of Biomedical Research2013，27，3： | 6 |
| 2 | Bioinformatics analysis for structure and function of CPR of Plasmodium falciparum显示文摘Objective:To analyse the structure and function of NADPH-cytochrome p450 reductase(CYPOR or CPR) from Plasmodium falciparum(Pf),and to predict its’ drug target and vaccine target. Methods:The structure,function,drug target and vaccine target of CPR from Plasmodium falciparum were analyzed and predicted by bioinformatics methods.Results:PfCPR,which was older CPR,had close relationship with the CPR from other Plasmodium species,but it was distant from its hosts,such as Homo sapiens and Anopheles.PfCPR was located in the cellular nucleus of Plasmodium falciparum.335aa-352aa and 591aa - 608aa were inserted the interior side of the nuclear membrane,while 151aa-265aa was located in the nucleolus organizer regions.PfCPR had 40 function sites and 44 protein-protein binding sites in amino acid sequence.The teriary structure of laa-700aa was forcep-shaped with wings.15 segments of PfCPR had no homology with Homo sapien CPR and most were exposed on the surface of the protein.These segments had 25 protein-protein binding sites.While 13 other segments all possessed function sites. Conclusions:The evolution or genesis of Plasmodium falciparum is earlier than those of Homo sapiens.PfCPR is a possible resistance site of antimalarial drug and may involve immune evasion, which is associated with parasite of sporozoite in hepatocytes.PfCPR is unsuitable as vaccine target,but it has at least 13 ideal drug targets. | Zhigang Fan Lingmin Zhang Guogang Yan Qiang Wu Xiufeng Gan Saifeng Zhong Guifen Lin | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，2： | 3 |
| 3 | 为从变形体 berghei 的氮的氧化物 synthase 和类似的蛋白质的结构和功能的生物信息学分析和预言显示文摘 <正>Objective:To search and analyze nitric oxide synthase(NOS) and similar proteins from Plasmodium berghei(Pb).Methods:The structure and function of nitric oxide synthase and similar proteins from Plasmodium berghei were analyzed and predicted by bioinformatics. Results:P6NOS were not available,but nicotinamide adenine dinucleotide 2’-phosphate reduced tetrasodium(NADPH)-cytochrome p450 reductase(CPR) were gained.PiCPR was in the nucleus of Plasmodium berghei,while 134aa-229aa domain was localize in nucleolar organizer. The amino acids sequence of P6CPR had the closest genetic relationship with Plasmodium vivax showing a 73%homology.The tertiary structure of PbCPR displayed the forcep-shape with wings,but no wings existed in the tertiary structure of its’ host,Mus musculus(Mm).137aa-200aa, 201aa-218aa,220aa-230aa,232aa-248,269aa-323aa,478aa-501aa and 592aa-606aa domains of P6CPR showed no homology with MmCPRs’,and all domains were exposed on the surface of the protein.Conclusions:NOS can’t be found in Plasmodium berghei and other Plasmodium species.PbCPR may be a possible resistance site of antimalarial drug,and the targets of antimalarial drug and vaccine.It may be also one of the mechanisms of immune evasion.This study on Plasmodium berghei may be more suitable to Plasmodium vivax.And137aa-200aa, 201aa-218aa,220aa-230aa,232aa-248,269aa-323aa,478aa-501aa and 592aa-606aa domains of PbCPR are more ideal targets of antimalarial drug and vaccine. | Zhigang Fan Gang Lv Lingmin Zhang Xiufeng Gan Qiang Wu Saifeng Zhong Guogang Yan Guifen Lin | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，1： | 2 |
| 4 | A splice variant of PGRP-LC required for expression of antimicrobial peptides in Anopheles gambiae显示文摘peptidoglycan 识别蛋白质(PGRP ) 的成员在在昆虫的免疫反应的不同表明的家庭戏必需品角色。PGRP-LC,在疟疾向量疟蚊属 gambiae 的这个家庭的七个成员之一生产了几拼接的变体。这里,我们显示出那 PGRP-LC,并且不 PGRP 家庭的另外的成员也不 Gram-negativebinding 蛋白质家庭的六个成员,在 A 在 Imd-Rel2 小径的控制下面为抗菌剂肽基因(如此的 asCEC1 和 GAM1 ) 的表示被要求。gambiae 房间线, 4a3A。PGRP-LCproduces 许多拼接能被分类进三亚群(LC1, LC2 和 LC3 ) 的变体,基于 carboxyl 终端序列。对一 LC1 亚群的 RNA 干扰导致了 CEC1 和 GAM1 的戏剧的减小。LC1a 并且到更小的程度 LC3a 的在表示上(theLC1 和 LC3 亚群的一个成员,分别地) 在房间线提高的 4a3A, CEC1 和 GAM1.These 的表示导致证明 LC1 亚群拼接变体在 A 为表示 ofCEC1 和 GAM1 是必要的。gambiae 房间排队。 | HUI LIN LINGMIN ZHANG CORALIA LUNA NGO T. HOA LIANGBIAO ZHENG | 2007 | Insect Science2007，14，3： | 2 |
| 5 | A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing显示文摘The typeⅡ prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR/Cas9) adaptive immune system is a cutting-edge genome-editing toolbox.However,its applications are still limited by its inefficient transduction.Herein,we present a novel gene vector,the zwitterionic polymer-inspired material with branched structure (ZEBRA) for efficient CRISPR/Cas9 delivery.Polo-like kinase 1 (PLK1) acts as a master regulator of mitosis and overexpresses in multiple tumor cells.The Cas9 and single guide sgRNA (sgRNA)-encoded plasmid was transduced to knockout Plk1 gene,which was expected to inhibit the expression of PLK1.Our studies demonstrated that ZEBRA enabled to transduce the CRISPR/Cas9 system with large size into the cells efficiently.The transduction with ZEBRA was cell line dependent,which showed~10-fold higher in CD44-positive cancer cell lines compared with CD44-negative ones.Furthermore,ZEBRA induced highlevel expression of Cas9 proteins by the delivery of CRISPR/Cas9 and efficient gene editing of Plk1 gene,and inhibited the tumor cell growth significantly.This zwitterionic polymerinspired material is an effective and targeted gene delivery vector and further studies are required to explore its potential in gene delivery applications. | Lingmin Zhang Langyu Yang Jionghua Huang Sheng Chen Chuangjia Huang Yinshan Lin Ao Shen ZhouYikang Zheng Wenfu Zheng Shunqing Tang | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，5： | 0 |
| 6 | Horizontal freezing study for cross passage of river-crossing tunnel显示文摘To determine the appropriate soft foundation treatment for a river-crossing tunnel,freezing reinforcement design and technology were introduced based on the channel tunnel design and construction practice.Through finite element analysis and engineering practices,two rows of horizontal perforated freezing pipes were designed and installed on both sides of a passage for tunnel rein-forcement,which produced the thickness and strength of frozen crust that satisfied the design requirements.These information are valuable for guiding the design and construction of river-crossing tunnels in coastal areas. | Ming Zhang LingMin Wang BaoWen Wang HongBao Lin | 2011 | Research in Cold and Arid Regions2011，3，4： | 0 |
| 7 | Manufacturer Rebates in a Green Supply Chain with Hybrid Production显示文摘This paper studies two manufacturer rebate strategies in a green supply chain.The results show that:1)Irrespective of the type of rebate strategy used,channel members benefit;2)Rebates for green products improve the green level of green products;3)The more significant the slippage effects are,the more beneficial the rebate strategies are,for channel members.On this basis,the model is expanded to consider asymmetric potential market demands and asymmetric product substitution rates,respectively.The results show that the product substitution rate does not affect the strategic preference of channel members,but the potential market demand does. | Zhibing LIN Lingmin DAI Mofan CHEN | 2022 | Journal of Systems Science and Information2022，10，2： | 0 |
| 8 | Preparation and Pharmacodynamic Evaluation of Kang Zhu Gan Lu Oral Proliposome, a New Hypoglycemic Tibetan Medicine显示文摘[Objectives]To effectively solve the problem of poor oral bioavailability of total flavonoids in Kang Zhu Gan Lu(ZYZH),a new hypoglycemic Tibetan solid preparation,ZYZH oral proliposome was prepared by modern preparation method,and its hypoglycemic effect was evaluated.[Methods]ZYZH proliposome was prepared by carrier deposition method,and its particle morphology and Zeta potential were detected.Taking encapsulation efficacy as indictor,the optimal prescription and preparation process of ZYZH oral proliposome were screened by orthogonal experiment.In addition,the hypoglycemic activity of ZYZH oral liposome in alloxan-induced hyperglycemic mice was studied.[Results]The repose angle,average particle size,encapsulation efficiency,and Zeta potential of ZYZH proliposome are 34.2°,(442.5±3.5)nm,67.7%and(-47.2±3.4)mV,respectively.Compared with the model group,the fasting blood glucose level of the hyperglycemic mice significantly declined(P<0.01),and their body weight changed insignificantly after four weeks of oral administration of ZYZH proliposome(P>0.05).[Conclusions]The preparation process of ZYZH oral proliposome is characterized by simple operation,high encapsulation efficiency and good stability.The new hypoglycemic Tibetan drug ZYZH oral liposome has obvious hypoglycemic effect on alloxan-induced hyperglycemic mice. | Weini DAI Yu MAO Lingmin LIN Xinran MI | 2019 | Medicinal Plant2019，10，5： | 0 |
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