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| 1 | The detection of HBV DNA with gold nanoparticle gene probes显示文摘Objective: Gold nanoparticle Hepatitis B virus (HBV) DNA probes were prepared, and their application for HBV DNA mea-surement was studied. Methods: Alkanethiol modified oligonucleotide was bound with self-made Au nanoparticles to formnanoparticle HBV DNA gene probes, through covalent binding of Au-S. By using a fluorescence-based method, the number ofthiol-derivatized, single-stranded oligonucleotides and their hybridization efficiency with complementary oligonucleotides in solutionwas determined. With the aid of Au nanoparticle-supported mercapto-modified oligonucleotides serving as detection probes, andoligonucleotides immobilized on a nylon membrane surface acting as capturing probes, HBV DNA was detected visually by sand-wich hybridization based on highly sensitive aggregation and silver staining. The modified nanoparticle HBV DNA gene probeswere also used to detect the HBV DNA extracted from serum in patients with hepatitis B. Results: Compared with bare Aunanoparticles, oligonucleotide modified nanoparticles had a higher stability in NaCl solution or under high temperature environ-ment and the absorbance peak of modified Au nanoparticles shifted from 520nm to 524nm. For Au nanoparticles, the maximaloligonucleotide surface coverage of hexaethiol 30-mer oligonucleotide was (132 ±10) oligonucleotides per nanoparticle, and thepercentage of hybridization strands on nanoparticles was (22 ±3%). Based on a two-probe sandwich hybridization/nanoparticleamplification /silver staining enhancement method, Au nanoparticle gene probes could detect as low as 10-11 mol/L composite HBVDNA molecules on a nylon membrane and the PCR products of HBV DNA visually. As made evident by transmission electron mi-croscopy, the nanoparticles assembled into large network aggregates when nanoparticle HBV DNA gene probes were applied to de-tect HBV DNA molecules in liquid. Conclusion:Our results showed that successfully prepared Au nanoparticle HBV DNA geneprobes could be used to detect HBV DNA directly. The detection-visuallized method has many advantages, including high sensitivity,simple operation and low cost. This technique has potential applications in many fields, especially in multi-gene detection chips. | Dong Xia Xiaoping Luo Qin Ning Qianghua Lu Kailun Yao Zuli Liu | 2007 | Journal of Nanjing Medical University2007，21，4： | 2 |
| 2 | Nanostructural Evolution:From One-Dimensional Tungsten Oxide Nanowires to Three-DimensionalFerberite Flowers显示文摘 | Weibing Hu Yimin Zhao Zuli Liu | 2008 | Chem Mater2008，20，17： | 1 |
| 3 | Electric properties of BiFeO3 films deposited on LaNiOa by sol-gel process 显示文摘 | Zuli Liu Hongri Liu Guihuan Du | 2006 | ApplPhys2006，100，00： | 1 |
| 4 | Electric and mag-netic properties of multiferroic (BiFe03)i^-(PbTi03)s filmsprepared by the sol-gel process 显示文摘 | Liu Hongri Liu Zuli Liu Qing | 2006 | Journal of PhysicsD : Applied Physics2006，39，6： | 1 |
| 5 | Nanostructural evolution:from one-dimensional tungsten oxide nanowires to three-dimensionalferberite flowers显示文摘 | HU Weibing ZHAO Yimin LIU Zuli | 2008 | Chem Mater2008，20，17： | 1 |
| 6 | Electric Properties of BiFeO3 Films Deposited on LaNiO3 by Sol-gel Process显示文摘 | Liu Zuli Liu Hongri Du Guihuan | 2006 | Appl Phys2006，，04： | 1 |
| 7 | Electric Properties of BiFeO3 Films Deposited on LaNiO3 by Sol-gel Process显示文摘 | Liu Zuli Liu Hongri Du Guihuan | 2006 | Journal of Applied Physics2006，100，00： | 1 |
| 8 | Electric and Magnetic Properties of Multiferroic (BiFeO3)1-x-(PbTiO3)x Films Prepared by the Sol-gel Process显示文摘 | Liu Hongri Liu Zuli Liu Qing | 2006 | J Phys D:Appl Phys2006，39，： | 1 |
| 9 | Preparation and characterization of polymer-coated core-shell structured magnetic mierobeads显示文摘 | Liu Zuli Ding Zhonghua Yao Kailun | 2003 | J Magn Magn Mater2003，265，： | 1 |
| 10 | Preparation and characterization of magnetic P (St-co-MAA-co-AM) microspheres 显示文摘 | Liu Zuli Yang Xiongbo Yao Kailun | 2006 | J Magn Magn Mater2006，302，： | 1 |
| 11 | Synthesis of magnetic nanoparticles in W/O microemulsion显示文摘 | Liu Zuli Wang Xin | 2004 | J Mater Sci2004，39，： | 1 |
| 12 | 显示文摘 | Liu Zuli Liu Hongri Du Guihuan | 2006 | J Appl Phys2006，100，4： | 1 |
| 13 | Synthesis and characterization of ultrafine well-dispersed magnetic nanoparticles 显示文摘 | Liu Zuli Wang Hanbin Lu Qianghua | 2004 | J Magn Magn Mater2004，283，： | 1 |
| 14 | 显示文摘 | Wang Guoqiang Liu Zuli | 2000 | Chinese J Huazhong Univ of Sci & Tech2000，28，7： | 1 |
| 15 | Ferroelectric properties of BiFeO3films grown by sol-gel process显示文摘 | Liu Hongri Liu Zuli Liu Qing | 2006 | Thin Solid Film2006，500，： | 1 |
| 16 | 显示文摘 | Liu Hongri Liu Zuli Liu Qing | 2006 | Thin Solid Films2006，，500： | 1 |
| 17 | Ferroelectric properties of BiFeO3films grown by sol-gel process显示文摘 | Liu Hongri Liu Zuli Liu Qing | 2006 | Thin Solid Films2006，500，： | 1 |
| 18 | The deubiquitylase UCHL3 maintains cancer stem-like properties by stabilizing the aryl hydrocarbon receptor显示文摘Cancer stem cells(CSCs)exhibit highly aggressive and metastatic features and resistance to chemotherapy and radiotherapy.Aryl hydrocarbon receptor(AhR)expression varies among non-small cell lung cancers(NSCLCs),and the mechanisms that support abnormal AhR expression in CSCs remain elusive.Here,we identified ubiquitin carboxyl terminal hydrolase L3(UCHL3),a DUB enzyme in the UCH protease family,as a bona fide deubiquitylase of the AhR in NSCLC.UCHL3 was shown to interact with,deubiquitylate,and stabilize AhR in a manner dependent on its deubiquitylation activity.Moreover,we showed that UCHL3 promotes the stem-like characteristics and potent tumorigenic capacity of NSCLC cells.UCHL3 increased AhR stability and the binding of AhR to the promoter regions of the“stemness”genes ATP-binding cassette subfamily G member 2(ABCG2),KLF4,and c-Myc.Depletion of UCHL3 markedly downregulated the“stemness”genes ABCG2,KLF4,and c-Myc,leading to the loss of selfrenewal and tumorigenesis in NSCLCs.Furthermore,the UCHL3 inhibitor TCID induced AhR degradation and exhibited significantly attenuated efficacy in NSCLC cells with stem cell-like properties.Additionally,UCHL3 was shown to indicate poor prognosis in patients with lung adenocarcinoma.In general,our results reveal that the UCHL3 deubiquitylase is pivotal for AhR protein stability and a potential target for NSCLC-targeted therapy. | Lianlian Ouyang Bin Yan Yating Liu Chao Mao Min Wang Na Liu Zuli Wang Shouping Liu Ying Shi Ling Chen Xiang Wang Yan Cheng Ya Cao Desheng Xiao Lingqiang Zhang Shuang Liu Yongguang Tao | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 1 |
| 19 | IL4I1-driven AHR signature: a new avenue for cancer therapy显示文摘Aryl hydrocarbon receptor(AHR)was considered to be an important pan-tumor therapeutic target,but small molecule inhibitors targeting AHR target gene ID01 have failed in clinical trials.The recent paper published in Cell by Opitz et al.explained the failure of previous clinical trials and identified new therapeutic targets(Fig.1). | Zuli Wang Tiansheng Li Chao Mao Wenliang Liu Yongguang Tao | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 0 |
| 20 | Reactive DC Magnetron Sputtering Deposition of Copper Nitride Thin Film显示文摘一氮化三铜薄电影被 reactiveDC (直接水流)在玻璃底层上扔磁控管在 0.5 Pa N2 部分压力和不同 substratetemperatures.The 劈啪作响同样准备的电影,与X光检查衍射描绘了,原子力量显微镜学,andX光线光电子光谱学大小,显示出 CU3N 雏晶 withanti-Re03 结构的镇静的结构和结果的电影的细微氧化。CU3N 电影的晶体结构和生长率被底层温度强烈影响。CU3N 电影的比较喜欢的水晶的取向是(111 ) 并且(200 ) 在 RT, 100 座 deg C.These 山峰在 200 deg C 和 300deg C 腐烂了仅仅 Cu (111 ) 山峰被注意。在 100 deg C 的 Cu3N 电影的生长是为生产的最佳底层温度高质量(111 ) CU3N 电影。当玻璃底层的温度增加了时,估计在当抵抗力和 CU3N 的微坚硬拍摄时,增加的 18-30 nm/min 的范围的 CU3N 电影的免职率减少了。 | Xing'ao LI Zuli LIU Kailun YAO | 2007 | Journal of Materials Science & Technology2007，23，4： | 0 |
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