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| 1 | Virus-induced gene silencing(VIGS)in Chinese narcissus and its use in functional analysis of NtMYB3显示文摘Virus-induced gene silencing(VIGS)is a natural defense mechanism of plants,which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome.At present,the VIGS system mediated by Tobacco rattle virus(TRV)has been successfully established in many plant species.However,the VIGS system in Chinese narcissus had not been reported yet.In this paper,the first use of the TRV-VIGS system in Chinese narcissus is described in detail.By injecting pTRV2-GFP into narcissus leaves,we confirmed that TRV could infect narcissus and move in narcissus plants.After the inoculation of pTRV2-Nt PDS by two methods,phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced.Besides,pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3;the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis,which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS.These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3. | Ping Zhou Jiayu Peng Minjie Zeng Lixuan Wu Yuxin Fan Lihui Zeng | 2021 | Horticultural Plant Journal2021，7，6： | 4 |
| 2 | WheatGmap:a comprehensive platform for wheat gene mapping and genomic studies显示文摘Bulked segregant analysis(BSA)is an efficient and low-cost strategy that is widely used to identify causal genes in segregating populations.BSA-based methods,such as BSA sequencing(Wenger et al.,2010),bulked segregant RNA sequencing(BSR-seq)(del Viso et al.,2012),and MutMap(Abe et al.,2012),are powerful tools that can be used for rapidly discovering genetic markers and gene mapping.Although BSA is increasingly being used in wheat(Triticum aestivum)gene mapping efforts,few user-friendly BSA tools have been developed for researchers lacking a strong bioinformatics background.Here,we developed the web-based BSA platform WheatGmap(http://gffzz4d94446f6f524399soowk6qbkvkkf6u5p.ffgz.tsg.suse.edu.cn),which integrates multiple BSA mapping models and large amounts of public data to accelerate gene cloning and functional research and facilitate resource sharing. | Lichao Zhang Chunhao Dong Zhongxu Chen Lixuan Gui Cheng Chen Danping Li Zhencheng Xie Qiang Zhang Xueying Zhang Chuan Xia Xu Liu Xiuying Kong Jirui Wang | 2021 | Molecular Plant2021，14，2： | 4 |
| 3 | A Novel Ratiometric Fluorescent Probe for Highly Sensitive and Selective Detection of β-Galactosidase in Living Cells显示文摘β-Galactosidase,a glycoside hydrolase enzyme,has been proved to be an important biomarker of cell senescence and primary ovarian cancer.Effective detection of β-galactosidase has attracted wide attention.Herein,one ratiometric fluorescent probe has been successfully synthesized for detecting the β-galactosidase in living cells.The as-prepared probe exhibits two emission peaks at 490 nm and 530 nm,respectively,and the ratio of fluorescence intensities from the two emission peaks could be utilized to monitor the β-galactosidase.This present ratiometric fluorescent probe is,therefore,very promising for effective,sensitive,and selective detection of the β-galactosidase in living cells. | Min Chen Lixuan Mu Xingxing Cao Guangwei She Wensheng Shi | 2019 | Chinese Journal of Chemistry2019，37，4： | 3 |
| 4 | A-π-D-π-A small-molecule donors with different end alkyl chains obtain different morphologies in organic solar cells显示文摘For bulk-heterojunction organic solar cells, the morphology of the blend films highly influence the exciton dissociation and charge transport process. In this work, two novel A-π-D-π-A(A represents the acceptor unit and D represents the donor unit) backbone structure small molecular electron donors based on two dimensional conjugated naphtho[1,2-b:5,6-b']dithiophene(NDT) with different end alkyl chains, named as NDT-3T-EH and NDT-3T-O, have been designed and synthesized. The photovoltaic property of NDT-3T-O-based device is better than that of the NDT-3T-EH and the best efficiency reaches 7.06%, and the photovoltaic property of NDT-3T-EH reaches 6.11%. The difference in the performance should be attributed to the different morphology and phase separation resulted from the different crystallinity and aggregation ability of two donors. The results demonstrate that the optimized end alkyl chains can be used to design A-π-D-π-A backbone structure small molecular electron donors for smallmolecule organic solar cells. | Yanan Shi Chen Yang Huan Li Lixuan Liu Ruimin Zhou Wenjun Zou Zhen Wang Qiong Wu Dan Deng Jianqi Zhang Kun Lu Zhixiang Wei | 2019 | Chinese Chemical Letters2019，30，4： | 3 |
| 5 | Direct Growth of Graphene on Silicon by Metal-Free Chemical Vapor Deposition显示文摘The metal-free synthesis of graphene on singlecrystal silicon substrates, the most common commercial semiconductor, is of paramount significance for many technological applications. In this work, we report the growth of graphene directly on an upside-down placed,single-crystal silicon substrate using metal-free, ambientpressure chemical vapor deposition. By controlling the growth temperature, in-plane propagation, edge-propagation, and core-propagation, the process of graphene growth on silicon can be identified. This process produces atomically flat monolayer or bilayer graphene domains, concave bilayer graphene domains, and bulging few-layer graphene domains. This work would be a significant step toward the synthesis of large-area and layer-controlled, high-quality graphene on single-crystal silicon substrates. | Lixuan Tai Daming Zhu Xing Liu Tieying Yang Lei Wang Rui Wang Sheng Jiang Zhenhua Chen Zhongmin Xu Xiaolong Li | 2018 | Nano-Micro Letters2018，10，2： | 2 |
| 6 | INHIBITORY ROLE OF TRANSCRIPTION FACTOR COUP-TFII IN EXPRESSION OF HTERT IN HELA CELLS显示文摘To clone and identify the proteins involved in regulating the transcription of hTERT and study the role of genes in both hTERT transcription and telomerase activity. Methods The full cDNA of COUP-TFII was cloned from HeLa cDNA library by hTERT promoter-based yeast one-hybrid assay and then in-frame inserted into His-tag fusion expression vector pEK318. The His-tag COUP-TFII fusion proteins were purified by Ni-NTA chromatography. The interaction of COUP-TFII with hTERT promoter in vitro was identified by electrophoretic mobility shift assay and Footprint. The role of COUP-TFII in both hTERT transcription and telomerase activity were probed through Luciferase reporter assay, Northern blot, and TRAP-PCR ELISA. Results COUP-TFII could firmly bind to the downstream E-box and the other two binding sites in hTERT promoter. Luciferase reporter assay indicated COUP-TFII could suppress hTERT promoter activity and stable introduction of COUP-TFII into HeLa cells also decreased both endogenous hTERT transcription and telomerase activity. Conclusion The human COUP-TFII can firmly bind to hTERT promoter, and inhibit telomerase activity through decreasing hTERT transcription. It will greatly facilitate understanding of telomerase regulation in normal and cancer | QiangWang Zeng-liangBai: LiXuan LinHou BoZhang | 2004 | Chinese Medical Sciences Journal2004，19，3： | 2 |
| 7 | Distribution of phthalate esters in urban soils of subtropical city, Guangzhou, China显示文摘 | Feng Zeng Kunyan Cui Zhiyong Xie Lina Wu Danling Luo Lixuan Chen Yujun Lin Min Liu Gouxuan Sun | 2008 | Journal of Hazardous Materials2008，，2： | 2 |
| 8 | Orbital ordering and fluctuations in a kagome superconductor CsV_(3)Sb_(5)显示文摘Recently,competing electronic instabilities,including superconductivity and density-wave-like order,have been discovered in vanadium-based kagome metals AV_(3)Sb_(5)(A=K,Rb,Cs)with a nontrivial band topology.This finding stimulates considerable interest to study the interplay of these competing electronic orders and possible exotic excitations in the superconducting state.Here,we performed51V and133Cs nuclear magnetic resonance(NMR)measurements on a CsV_(3)Sb_(5)single crystal to clarify the nature of density-wave-like transition in these kagome superconductors.A first-order structural transition is unambiguously revealed below T_(s)~94 K by observing the sudden splitting of Knight shift in^(51)V NMR spectrum.Moreover,combined with^(133)Cs NMR spectrum,the present result confirms a three-dimensional structural modulation.By further analyzing the anisotropy of Knight shift and 1/T_(1)T at^(51)V nuclei,we proposed that the orbital order is the primary electronic order induced by the firstorder structural transition,which is supported by further analysis on electric field gradient at^(51)V nuclei.In addition,the evidence for possible orbital fluctuations is also revealed above T_(s).The present work sheds light on a rich orbital physics in kagome superconductors AV_(3)Sb_(5). | DianWu Song LiXuan Zheng FangHang Yu Jian Li LinPeng Nie Min Shan Dan Zhao ShunJiao Li BaoLei Kang ZhiMian Wu YanBing Zhou KuangLv Sun Kai Liu XiGang Luo ZhenYu Wang JianJun Ying XianGang Wan Tao Wu XianHui Chen | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，4： | 2 |
| 9 | TNP-ATP is Beneficial for Treatment of Neonatal HypoxiaInduced Hypomyelination and Cognitive Decline显示文摘Our previous study together with other investigations have reported that neonatal hypoxia or ischemia induces long-term cognitive impairment, at least in part through brain inflammation and hypomyelination. However, the detailed mechanisms are not fully understood.Here, we used a rodent model of neonatal hypoxia by subjecting postnatal day 0(P0) rat pups to systemic hypoxia(3.5 h). We found that neonatal hypoxia increased the glutamate content and initiated inflammatory responses at 4 h and 1 day after hypoxia, caused hypomyelination in the corpus callosum, and impaired hippocampus-dependent learning and memory when assessed 30–60 days after hypoxia. Interestingly, much of the hypoxia-induced brain damage was ameliorated by treatment with the ATP analogue 20,30-0-(2,4,6-trinitrophenyl)-adenosine 50-triphosphate(TNP-ATP; blocks all ionotropic P2X1-7 receptors),whereas treatment with pyridoxalphosphate-6-azophenyl-20,40-disulphonic acid(PPADS; inhibits P2X1-3 and P2X5-7 receptors) was less neuroprotective. Our data indicated that activation of ionotropic ATP receptors might be partially, if not fully, involved in glutamate deregulation,neuroinflammation, hypomyelination, and cognitive dysfunction after neonatal hypoxia. | Jie Xiao Yilong Huang Xia Li Longjun Li Ting Yang Lixuan Huang Ling Yang Hong Jiang Hongchun Li Fan Li | 2016 | Neuroscience Bulletin2016，32，1： | 2 |
| 10 | Bose-Einstein condensation of an ideal Bose gas trapped in any dimension 显示文摘 | Chen Lixuan Yan Zijun Li Mingzhe | 1998 | J Phys A: Math Gen1998，31，41： | 1 |
| 11 | Porous stainless steel supported magnetite crystalline membranes for hexavalent chromium removal from aqueous solutions显示文摘 | TANG Wenzhi PENG Zhichao LI Lixuan | | 0，，： | 1 |
| 12 | Recent advances in modification strategies of silicon-based lithiumion batteries显示文摘As potential alternatives to graphite,silicon(Si)and silicon oxides(SiO_(x))received a lot of attention as anode materials for lithiumion batteries owing to their relatively low working potentials,high theoretical specific capacities,and abundant resources.However,the commercialization of Si-based anodes is greatly hindered by their massive volume expansion,low conductivity,unstable solid electrolyte interface(SEI),and low initial Coulombic efficiency(ICE).Continuous endeavors have been devoted to overcoming these challenges to achieve practical usage.This review is centered on the major challenges and latest developments in the modification strategies of Si-based anodes,including structure optimization,surface/interface regulation,novel binders,and innovative design of electrolyte.Finally,outlooks and perspectives of Si-based anodes for future development are presented. | Wenlei Wang Yu Wang Lixuan Yuan Chaolin You Junwei Wu Lili Liu Jilei Ye Yunling Wu Lijun Fu | 2023 | Nano Research2023，16，3： | 1 |
| 13 | Activation of Akt/ FoxO signaling pathway contributes to induction of neu- roprotection against transient global cerebral ischemia by hypoxic pre-conditioning in adult rats 显示文摘 | Zhan Lixuan Wang Tao Li Wen | 2010 | J Neurochem2010，114，3： | 1 |
| 14 | Offshore wind energy potential in China: Under technical, spatial and economic constraints显示文摘 | Lixuan Hong Bernd M?ller | 2011 | Energy2011，，7： | 1 |
| 15 | Application of controlled- release nitrogen fertilizer decreased methane emission in transgenic rice from a paddy soil显示文摘 | Zhou Wenlin Lou yunsheng Ren Lixuan | 2014 | Water Air Soil Pollut2014，，225： | 1 |
| 16 | Occurrence and distribution of phthalate esters in riverine sediments from the Pearl River Delta region, South China显示文摘 | Hong Liu Kunyan Cui Feng Zeng Lixuan Chen Yating Cheng Huiru Li Shuocong Li Xi Zhou Fang Zhu Gangfeng Ouyang Tiangang Luan Zunxiang Zeng | 2014 | Marine Pollution Bulletin2014，，： | 1 |
| 17 | Predicting protein structural class based on multi-features fusion显示文摘 | CHEN Chao CHEN Lixuan ZOU Xiaoyong | 2008 | Journal of Theoretical Biology2008，253，： | 1 |
| 18 | Baicalin attenuates inflammation by inhibiting NF-kappaB activation in cigarette smoke induced inflammato- ry models显示文摘 | Lixuan Z Jingcheng D Wenqin Y | 2010 | Pulm Pharmacol Ther2010，23，5： | 1 |
| 19 | Risk prediction of esophageal squamous-cell carcinoma with common genetic variants and lifestyle factors in Chinese population显示文摘 | Jiang Chang Ying Huang Lixuan Wei Baoshan Ma Xiaoping Miao Yun Li Zhibin Hu Dianke Yu Weihua Jia Yu Liu Wen Tan Zhonghu He Yang Ke Tangchun Wu Hongbing Shen Yixin Zeng Chen Wu Dongxin Lin | 2013 | Carcinogenesis2013，，8： | 1 |
| 20 | Mg,Ti-base surface integrated layer and bulk doping to suppress lattice oxygen evolution of Ni-rich cathode material at a high cut-off voltage显示文摘The Nickel-rich layered cathode materials charged to 4.5 V can obtain a specific capacity of more than 200 m Ah g^(-1).However,the nickel-rich layered cathode materials suffer from the severe capacity fade during high-voltage cycling,which is related to the phase transformation and the surface sides reactions caused by the lattice oxygen evolution.Here,the simultaneous construction of a Mg,Ti-based surface integrated layer and bulk doping through Mg,Ti surface treatment could suppress the lattice oxygen evolution of Nirich material at deep charging.More importantly,Mg and Ti are co-doped into the particles surface to form an Mg_(2)TiO_(4) and Mg_(0.5–x)Ti_(2–y)(PO_(4))_(3) outer layer with Mg and Ti vacancies.In the constructed surface integrated layer,the reverse electric field in the Mg_(2)TiO_(4) effectively suppressed the outward migration of the lattice oxygen anions,while Mg_(0.5–x)Ti_(2–y)(PO_(4))_(3) outer layer with high electronic conductivity and good lithium ion conductor could effectively maintained the stability of the reaction interface during highvoltage cycling.Meanwhile,bulk Mg and Ti co-doping can mitigate the migration of Ni ions in the bulk to keep the stability of transition metal–oxygen(M-O)bond at deep charging.As a result,the NCM@MTP cathode shows excellent long cycle stability at high-voltage charging,which keep high capacity retention of 89.3%and 84.3%at 1 C after 200 and 100 cycles under room and elevated temperature of 25 and 55°C,respectively.This work provides new insights for manipulating the surface chemistry of electrode materials to suppress the lattice oxygen evolution at high charging voltage. | Fan Peng Youqi Chu Yu Li Qichang Pan Guangchang Yang Lixuan Zhang Sijiang Hu Fenghua Zheng Hongqiang Wang Qingyu Li | 2022 | Journal of Energy Chemistry2022，31，8： | 1 |
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