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| 1 | A mouse model for HBV immunotolerance and immunotherapy显示文摘一个适当小动物模型的缺乏仍然是一个主要障碍因为学习 immunotolerance 和 immunopathogenesis 由肝炎 B 病毒(HBV ) 导致了感染。在这研究,我们与 recombinant 在感染以后与持续 HBV viremia 报导一个老鼠模型带一个可复制的 HBV 染色体(AAV/HBV ) 的联系 adeno 的病毒(AAV ) 。类似于临床的 HBV 搬运人,感染 AAV/HBV 的老鼠为对 HBV 表面抗原(HBsAg ) 的抗体是 sero 否定的。有面对铝助手疫苗的常规 HBV 的免疫没能在这些老鼠对 HBV 得到有免疫力的回答。为了识别一支疫苗,那能潜在地围绕这忍耐, TLR9 收缩筋 CpG 作为一个助手被加到 HBsAg。有 HBsAg/CpG 的老鼠的种痘导致了 viremia,而且强壮的抗体生产和 T 房间回答的不仅清理。而且, DNA 复制和 HBV 的蛋白质表示显著地在 AAV/HBV-infected 鼠标的肝被减少。因此, AAV/HBV-infected 老鼠可以作为一个柔韧的模型被使用调查 HBV immunotolerance 并且为开发新奇免疫疗法根除的内在的机制 HBV 感染。 | Dan Yang Longchao Liu Danming Zhu Hua Peng Lishan Su Yang-Xin Fu Liguo Zhang | 2014 | Cellular & Molecular Immunology2014，11，1： | 25 |
| 2 | Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population显示文摘Background: Runs of homozygosity(ROHs) are homozygous segments of the genome where the two haplotypes inherited from the parents are identical. The current availability of genotypes for a very large number of single nucleotide polymorphisms(SNPs) is leading to more accurate characterization of ROHs in the whole genome. Here,we investigated the occurrence and distribution of ROHs in 3,692 Large White pigs and compared estimates of inbreeding coefficients calculated based on ROHs(FROH), homozygosity(FHOM), genomic relationship matrix(FGRM)and pedigree(FPED). Furthermore, we identified genomic regions with high ROH frequencies and annotated their candidate genes.Results: In total, 176,182 ROHs were identified from 3,569 animals, and all individuals displayed at least one ROH longer than 1 Mb. The ROHs identified were unevenly distributed on the autosomes. The highest and lowest coverages of Sus scrofa chromosomes(SSC) by ROH were on SSC14 and SSC13, respectively. The highest pairwise correlation among the different inbreeding coefficient estimates was 0.95 between FROH_totaland FHOM, while the lowest was-0.083 between FGRMand FPED. The correlations between FPEDand FROHusing four classes of ROH lengths ranged from 0.18 to 0.37 and increased with increasing ROH length, except for ROH > 10 Mb. Twelve ROH islands were located on four chromosomes(SSC1, 4, 6 and 14). These ROH islands harboured genes associated with reproduction, muscular development, fat deposition and adaptation, such as SIRT1, MYPN, SETDB1 and PSMD4.Conclusion: FROHcan be used to accurately assess individual inbreeding levels compared to other inbreeding coefficient estimators. In the absence of pedigree records, FROHcan provide an alternative to inbreeding estimates.Our findings can be used not only to effectively increase the response to selection by appropriately managing the rate of inbreeding and minimizing the negative effects of inbreeding depression but also to help detect genomic regions with an effect on traits under selection. | Liangyu Shi Ligang Wang Jiaxin Liu Tianyu Deng Hua Yan Longchao Zhang Xin Liu Hongmei Gao Xinhua Hou Lixian Wang Fuping Zhao | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 10 |
| 3 | Atomic Fe-Zn dual-metal sites for high-efficiency pH-universal oxygen reduction catalysis显示文摘An effective electrocatalyst being highly active in all pH range for oxygen reduction reaction(ORR)is crucial for energy conversion and storage devices.However,most of the high-efficiency ORR catalysis was reported in alkaline conditions.Herein,we demonstrated the preparation of atomically dispersed Fe-Zn pairs anchored on porous N-doped carbon frameworks(Fe-Zn-SA/NC),which works efficiently as ORR catalyst in the whole pH range.It achieves high half-wave potentials of 0.78,0.85 and 0.72 V in 0.1 M HClO4,0.1 M KOH and 0.1 M phosphate buffer saline(PBS)solutions,respectively,as well as respectable stability.The performances are even comparable to Pt/C.Furthermore,when assembled into a Zn-air battery,the high power density of 167.2 mWcm−2 and 120 h durability reveal the feasibility of Fe-Zn-SA/NC in real energy-related devices.Theoretical calculations demonstrate that the superior catalytic activity of Fe-Zn-SA/NC can be contributed to the lower energy barriers of ORR at the Fe-Zn-N6 centers.This work demonstrates the potential of Fe-Zn pairs as alternatives to the Pt catalysts for efficient catalytic ORR and provides new insights of dual-atom catalysts for other energy conversion related catalytic reactions. | Jie Xu Shuhua Lai Defeng Qi Min Hu Xianyun Peng Yifan Liu Wei Liu Guangzhi Hu Heng Xu Fan Li Chao Li Jia He Longchao Zhuo Jiaqiang Sun Yuan Qiu Shusheng Zhang Jun Luo Xijun Liu | 2021 | Nano Research2021，14，5： | 6 |
| 4 | Evaluation of the potential risks of heavy metal contamination in rice paddy soils around an abandoned Hg mine area in Southwest China显示文摘To assess the potential ecological and health risks of trace elements(Hg,Cd,As,Mn,Sb,Pb,Cu,Ni,Cr,and Zn),a total of 138 soil samples from rice paddies were collected during the rice harvest season in the Wanshan mining area,Guizhou Province,Southwest China.Factors of the pollution load index(PLI),geo-accumulation index(I-Geo),enrichment factor(EF),and risk index(RI)were determined.High concentrations of Hg,Sb,As,Zn,Cd,Cu,and Mn were observed in the soils.The PLI,I-Geo,and EF results all showed high levels of contamination by Hg and Sb and moderate levels of contamination by As,Pb,Zn,Cu,Cd,and Mn.There was no significant contamination from Ni and Cr.The RI was very high,with Hg as the dominant pollutant,as expected,indicating that the historical large-scale Hg mining,as well as artisanal mining,has had a significant impact on the Wanshan area.Moreover,coal combustion,manganese factories,and the use of agrochemicals by the local population could also have an impact on the soil through the introduction of heavy metal loads.To address the current state of contamination,pollutant remediation and the regulation control of the anthropogenic activities in Wanshan are urgently needed. | Bayou Adlane Zhidong Xu Xiaohang Xu Longchao Liang Jialiang Han Guangle Qiu | 2020 | Acta Geochimica2020，39，1： | 5 |
| 5 | Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing显示文摘Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still limited.Results: Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver,muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine(A-to-G) conversion. Most A-to-G sites were located in noncoding regions and generally had low editing levels. In total, 151 A-to-G sites were detected in coding regions(CDS), including 94 sites that could lead to nonsynonymous amino acid changes. We provide further evidence supporting a previous observation that pig transcriptomes are highly editable at PRE-1 elements. The number of A-to-G editing sites ranged from 4155(muscle) to 25001(brain) across the seven tissues. The expression levels of the ADAR enzymes could explain some but not all of this variation across tissues. The functional analysis of the genes with tissuespecific editing sites in each tissue revealed that RNA editing might play important roles in tissue function.Specifically, more pathways showed significant enrichment in the fat and liver than in other tissues, while no pathway was enriched in the muscle.Conclusions: This study identified a total of 74863 nonredundant RNA editing sites in seven tissues and revealed the potential importance of RNA editing in tissue function. Our findings largely extend the porcine editome and enhance our understanding of RNA editing in swine. | Yuebo Zhang Longchao Zhang Jingwei Yue Xia Wei Ligang Wang Xin Liu Hongmei Gao Xinhua Hou Fuping Zhao Hua Yan Lixian Wang | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 4 |
| 6 | Ice-templated porous tungsten and tungsten carbide inspired by natural wood显示文摘The structures of tungsten and tungsten carbide scaffolds play a key role in determining the properties of their infiltrated composites for multifunctional applications.However,it is challenging to construct and control the architectures by means of self-assembly in W/WC systems because of their large densities.Here we present the development of unidirectionally porous architectures,with high porosities exceeding 65 vol.%,for W and WC scaffolds which in many respects reproduce the design motif of natural wood using a direct ice-templating technique.This was achieved by adjusting the viscosities of suspensions to retard sedimentation during freezing.The processing,structural characteristics and mechanical properties of the resulting scaffolds were investigated with the correlations between them explored.Quantitative relationships were established to describe their strengths based on the mechanics of cellular solids by taking into account both inter-and intra-lamellar pores.The fracture mechanisms were also identified,especially in light of the porosity.This study extends the effectiveness of the ice-templating technique for systems with large densities or particle sizes.It further provides preforms for developing new natureinspired multifunctional materials,as represented by W/WC-Cu composites. | Yuan Zhang Guoqi Tan Da Jiao Jian Zhang Shaogang Wang Feng Liu Zengqian Liu Longchao Zhuo Zhefeng Zhang Sylvain Deville Robert O.Ritchie | 2020 | Journal of Materials Science & Technology2020，42，10： | 4 |
| 7 | Chemical characterization and sources of PM2.5 at 12-h resolution in Guiyang, China显示文摘The increasing emission of primary and gaseous precursors of secondarily formed atmospheric particulate matter due to continuing industrial development and urbanization are leading to an increased public awareness of environmental issues and human health risks in China.As part of a pilot study, 12-h integrated fine fraction particulate matter(PM_(2.5) filter samples were collected to chemically characterize and investigate the sources of ambient particulate matter in Guiyang City, Guizhou Province, southwestern China. Results showed that the 12-h integrated PM_(2.5) concentrations exhibited a daytime average of 51 ± 22 lg m^(-3)(mean ± standard deviation) with a range of 17–128 lg m^(-3) and a nighttime average of55 ± 32 lg m~(^(-3)) with a range of 4–186 lg m^(-3). The 24-h integrated PM_(2.5) concentrations varied from 15 to157 lg m~(^(-3)), with a mean value of 53 ± 25 lg m^(-3), which exceeded the 24-h PM_(2.5) standard of 35 lg m^(-3) set by USEPA, but was below the standard of 75 lg m^(-3), set by China Ministry of Environmental Protection. Energy-dispersive X-ray fluorescence spectrometry(XRF) was applied to determine PM_(2.5) chemical element concentrations. The order of concentrations of heavy metals in PM_(2.5) were iron(Fe) [ zinc(Zn) [ manganese(Mn) [ lead(Pb) [ arsenic(As) [ chromium(Cr). The total concentration of 18 chemical elements was 13 ± 2 lg m^(-3),accounting for 25% in PM_(2.5), which is comparable to other major cities in China, but much higher than cities outside of China. | Longchao Liang Na Liu Matthew S. Landis Xiaohang Xu Xinbin Feng Zhuo Chen Lihai Shang Guangle Qiu | 2018 | Acta Geochimica2018，37，2： | 4 |
| 8 | Bioinspired tungsten-copper composites with Bouligand-type architectures mimicking fish scales显示文摘The microscopic Bouligand-type architectures of fish scales demonstrate a notable efficiency in enhancing the damage tolerance of materials;nevertheless,it is challenging to reproduce in metals.Here bioinspired tungsten-copper composites with different Bouligand-type architectures mimicking fish scales were fabricated by infiltrating a copper melt into woven contextures of tungsten fibers.These composites exhibit a synergetic enhancement in both strength and ductility at room temperature along with an improved resistance to high-temperature oxidization.The strengths were interpreted by adapting the classical laminate theory to incorporate the characteristics of Bouligand-type architectures.In particular,under load the tungsten fibers can reorient adaptively within the copper matrix by their straightening,stretching,interfacial sliding with the matrix,and the cooperative kinking deformation of fiber grids,representing a successful implementation of the optimizing mechanisms of the Bouligand-type architectures to enhance strength and toughness.This study may serve to promote the development of new high-performance tungsten-copper composites for applications,e.g.,as electrical contacts or heat sinks,and offer a viable approach for constructing bioinspired architectures in metallic materials. | Yuan Zhang Guoqi Tan Mingyang Zhang Qin Yu Zengqian Liu Yanyan Liu Jian Zhang Da Jiao Faheng Wang Longchao Zhuo Zhefeng Zhang Robert O.Ritchie | 2022 | Journal of Materials Science & Technology2022，，1： | 3 |
| 9 | The landscape of chromatin accessibility in skeletal muscle during embryonic development in pigs显示文摘Background:The development of skeletal muscle in pigs during the embryonic stage is precisely regulated by transcriptional mechanisms,which depend on chromatin accessibility.However,how chromatin accessibility plays a regulatory role during embryonic skeletal muscle development in pigs has not been reported.To gain insight into the landscape of chromatin accessibility and the associated genome-wide transcriptome during embryonic muscle development,we performed ATAC-seq and RNA-seq analyses of skeletal muscle from pig embryos at 45,70 and 100 days post coitus(dpc).Results:In total,21,638,35,447 and 60,181 unique regions(or peaks)were found across the embryos at 45 dpc(LW45),70 dpc(LW70)and 100 dpc(LW100),respectively.More than 91%of the peaks were annotated within−1 kb to 100 bp of transcription start sites(TSSs).First,widespread increases in specific accessible chromatin regions(ACRs)from embryos at 45 to 100 dpc suggested that the regulatory mechanisms became increasingly complicated during embryonic development.Second,the findings from integrated ATAC-seq and RNA-seq analyses showed that not only the numbers but also the intensities of ACRs could control the expression of associated genes.Moreover,the motif screening of stage-specific ACRs revealed some transcription factors that regulate muscle developmentrelated genes,such as MyoG,Mef2c,and Mef2d.Several potential transcriptional repressors,including E2F6,OTX2 and CTCF,were identified among the genes that exhibited different regulation trends between the ATAC-seq and RNA-seq data.Conclusions:This work indicates that chromatin accessibility plays an important regulatory role in the embryonic muscle development of pigs and regulates the temporal and spatial expression patterns of key genes in muscle development by influencing the binding of transcription factors.Our results contribute to a better understanding of the regulatory dynamics of genes involved in pig embryonic skeletal muscle development. | Jingwei Yue Xinhua Hou Xin Liu Ligang Wang Hongmei Gao Fuping Zhao Lijun Shi Liangyu Shi Hua Yan Tianyu Deng Jianfei Gong Lixian Wang Longchao Zhang | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 2 |
| 10 | Yield and quality properties of silage maize and their influencing factors in China显示文摘Silage maize(Zea mays L.) is one of the most important forages in the world, and its yield and quality properties are critical parameters for livestock production and assessment of forage values. However, relationships between its yield and quality properties and the controlling factors are not well documented. In this study, we collected 5,663 observations from 196 publications across the country to identify the relationships between yield and quality properties of silage maize and to assess the impact of management practices and climatic factors on its yield and quality in China. The average dry matter yield of silage maize was(19.98±6.93) Mg ha^(-1), and the average value of crude protein, ether extract, crude ash, crude fiber, acid detergent fiber, neutral detergent fiber, nitrogen-free extract, and relative feed value was 7.86%±1.71%, 2.53%±1.01%, 5.05%±1.66%,23.97%±6.34%, 27.62%±7.12%, 51.60%±9.85%, 59.68%±7.72%, and 131.17±31.49, respectively. In general, its nutritive value decreased as its yield increased. Increasing planting density could increase the yield but inhibit the nutritive values, while increasing fertilization could benefit the nutritive values. Geographically, the yield increased and the nutritive value decreased from warm(south) to cold(north) regions. The length of growth duration was a major controlling factor for the patterns of these properties. Our findings provide insights for police-makers to make strategy for achieving high yield and good quality of silage maize and help local people to implement better management practices. | Mengying Zhao Yinping Feng Yue Shi Haihua Shen Huifeng Hu Yongkai Luo Longchao Xu Jie Kang Aijun Xing Shaopeng Wang Jingyun Fang | 2022 | Science China(Life Sciences)2022，65，8： | 2 |
| 11 | Thermal properties and combustion characterization of nylon 6/MgAl-LDH nanocomposites via organic modification and melt intercalation显示文摘 | Longchao Du Baojun Qu Ming Zhang | 2006 | Polymer Degradation and Stability2006，，3： | 2 |
| 12 | Hierarchical ultrafine-grained/nanocystalline Al-based bulk alloy with high strength and large plasticity显示文摘 | Longchao Zhuo Enhuai Yin Hui Wang Tao Zhang | 2011 | Intermetallics2011，，： | 1 |
| 13 | Porcine methionine sulfoxide reductase B3:molecular cloning,tissue-specific expression profiles,and polymorphisms associated with ear size in Sus scrofa显示文摘Background: In Sus scrofa, methionine sulfoxide reductase B3(MSRB3) is a crucial candidate gene for ear size, and an important conformational trait of pig breeds. However, challenges in MSRB3 c DNA amplification have prevented further identification of MSRB3 allelic variants influencing pig ear size.Results: We cloned a full-length c DNA sequence of porcine MSRB3 by rapid-amplification of c DNA ends. The3,765-bp gene contained a 5'-untranslated region(UTR)(190 bp), a coding region(552 bp), and a 3'-UTR(3,016 bp) and shared 84 %, 84 %, 87 %, 86 %, and 70 % sequence identities with human, orangutan, mouse, chicken, and zebrafish,respectively. The gene encoded a 183-amino acid protein, which shared 88 %, 91 %, 89 %, 86 %, and 67 % identities with human, orangutan, mouse, chicken, and zebrafish, respectively. Tissue expression analysis using q RT-PCR revealed that MSRB3 was expressed in the heart, liver, lung, kidney, spleen, ear, muscle, fat, lymph, skeletal, and hypothalamic tissues. Three single nucleotide polymorphisms(SNPs) were identified in MSRB3: c.-735 C > T in the 5' flanking region,c.2571 T > C in the 3'-UTR, and a synonymous mutation of c.484 T > C in the coding region. The SNPs c.-735 C > T and c.2571 T > C were significantly associated with ear size in a Large White × Minzhu F2 population other than in Beijing Black pigs. Subsequently, at SNP c.-735 C > T, the m RNA of MSRB3 was significantly higher expressed in ears of individuals with the TT genotype(Minzhu) than those with CC(Large White).Conclusions: The porcine MSRB3 owned a 3,765-bp full-length c DNA sequence and was detected to express in ear tissue. Two SNPs of this gene were shown to be significantly associated with ear size in a Large White × Minzhu intercross population instead of Beijing Black pig population. What's more, the individuals with higher m RNA expression of MSRB3 have larger ear sizes. These results provide useful information for further functional analyses of MSRB3 influencing ear size in pigs. | Yuebo Zhang Jing Liang Longchao Zhang Ligang Wang Xin Liu Hua Yan Kebin Zhao Huibi Shi Tian Zhang Na Li Lei Pu Lixian Wang | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 1 |
| 14 | Flammability characteristics and synergistic effect of hydrotaleite with microeneapsulated red phosphorus in halogen-free flame retardant EVA composite 显示文摘 | Longchao Du Baojun Qu Zhenjin Xu | 2006 | Polymer Degradation and Stability2006，91，： | 1 |
| 15 | Structural characterization and thermal and mechanical properties of poly (propylene carbonate)/MgAl-LDH exfoliation nano-composite via solution intercalation 显示文摘 | Longchao D Bap-Jun Q Yue-Zhong M | 2006 | Composites Science and Technology2006，66，： | 1 |
| 16 | Flammability characteristics and synergistic effect of hydrotalcite with microencapsulated red phosphorus in halogen-free flame retardant EVA composite显示文摘 | Longchao Du Baojun Qu Zhenjin Xu | 2006 | Polymer Degradafion and Stahility2006，91，： | 1 |
| 17 | Structural characterization and thermal and mechanical properties of poly( propylene carbonate) MgAI-LDH exfoliation nanocomposite via solution intercalation 显示文摘 | Du Longchao Qu Baojun Meng Yuezhong | 2006 | Composites Science and Technology2006，66，： | 1 |
| 18 | Flammability characteristics and synergistic effect of hydrotalcite with microencapsulated red phosphorus in halogen-free flame retardant EVA composite显示文摘 | Longchao Du Baojun Qu Zhengjin Xu | 2006 | Polymer Degradation and Stability2006，91，2006： | 1 |
| 19 | Flammability characteristics and synergistic effect of hydrotalcite with microencapsulated red phosphorus in halogen-free flame retardant EVA composite 显示文摘 | DU Longchao QU Baojun XU Zhenjin | 2006 | Polym Degrad Stability2006，91，5： | 1 |
| 20 | Genome-wide search for candidate genes determining vertebrae number in pigs显示文摘Longer porcine carcasses may be expected to have more vertebrae. Therefore, vertebrae number in pigs is an economically important trait. To examine the genetic basis of this trait, we genotyped 578 F_2 Large White ?Minzhu pigs using the Porcine SNP60K Bead Chip. A genome-wide association study(GWAS) identified 36 significant single nucleotide polymorphisms(SNPs) on the chromosomes SSC1(294.28–300.32 Mb) and SSC7(102.22–109.39 Mb). A 6.04-Mb region that contained all 13 significant SNPs on SSC1 also contained the gene NR6A1, previously reported to influence the number of vertebrae in pigs. However, the reported putative casual mutation of NR6A1 c.748 C > T showed no genome-wide significant association with the trait, suggesting it was not a causal mutation in our population. The remaining 23 significant SNPs on SSC7 were concentrated in a 7.17-Mb region, which was within a quantitative trait locus interval for number of vertebrae. TMED10 was the closest gene to the most significant SNP and might be a candidate.Haplotype sharing and block analysis refined the QTL to an interval of about 3 Mb containing 29 candidate genes.Of these 29 genes, the previously reported possible casual mutation of VRTN g.19034 A > C was not found to be a causal mutation in our population. Exploration of these genes via additional genetic and functional studies in mammals revealed that TGFβ3 could be a good candidate on SSC7. A mutation of TGFβ3 c.1749 G > A was detected by GWAS and could be proposed as a candidate causal mutation, or as closely linked to a causal mutation, for the number of vertebrae in pigs. | Longchao ZHANG Jingwei YUE Xin LIU Jing LIANG Kebin ZHAO Hua YAN Na LI Lei PU Yuebo ZHANG Huibi SHI Ligang WANG Lixian WANG | 2017 | Frontiers of Agricultural Science and Engineering2017，4，3： | 1 |
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