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| 1 | Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice显示文摘AIM: To explore the mechanism of the Sijunzi decoction and another Chinese herbal recipe (SRRS) based mainly on the Sijunzi decoction in treatment of gastric cancer.METHODS: A human gastric adenocarcinoma cell line SGC7901 grafted onto nude mouse was used as the animal model. The mice were divided into 3 groups, one control and the two representative experimental conditions. Ahimals in the two experimental groups received either Sijunzi decoction or SRRS over a 40-day period starting at 1st day after grafting. Control animals received saline on an identical schedule. Animals were killed 41 days after being grafted.The effect of therapy was assessed by two ways: (1)tumor size was periodically measured during the life of the animals; (2) tumor weight was determined by a electron balance immediately after the animals killed. For detection of apoptotic cells, apoptotic indices(AI) were examined by the terminal deoxynucleotidyl transferase-mediated deoxyuddine tdphosphate fluorescence nick end labeling (TUNEL) method.Morphological alterations were observed with electron microscopy. S-P immunohistochemical method was used to detect the expression of Ki-67 in xenografts. Expression of bcl-2 and p53 was semiquantitatively detected using a reverse transcriptase-polymerase chain reaction (RT-PCR)technique.RESULTS: When compared with controls, tumor growth (size and weight) was significantly inhibited by treatment with the Sijunzi decoction (P<0.05) or SRRS (P<0.01). The tumor inhibitory rate in the Sijunzi decoction group was 34.33 % and SRRS group 46.53 %. AI of human gastric cancer xenografts in nude mice was significantly increased to 16.24±3.21% using TUNEL method and 11.38±6.46 % by FACScan in the Sijunzi decoction group compared with the controls (TUNEL: 2.63±1.03 %, P<0.01; FACScan: 7.15± 1.32 %, P<0.05). SRRS group was also found a significantly increased AI by using TUNEL method and flow cytometry analysis compared with the controls (TUNEL: 13.18±3.05 %,P<0.05; FACScan: 11.58±5.71% (P<0.05). Under electron microscope, cell shrinkage, nuclear chromatin condensation,formation of membrane blebs and apoptotic bodies were frequently observed in Sijunzi decoction group and SRRS group. The average labeling index (LI) for Ki-67 in SRRS group was significantly decreased to 8.43±2.22 % compared with the control group (10.37±t.91%) (P<0.05). The average labeling index for Ki-67 in sijunzi decoction group was 7.95±2.54 % which was lower than that of the control group, but showed no significance (P=0.07). The expression level of p53 mRNA was lower in both Sijunzi decoction group and SRRS group than that in control group (P<0.05; P<0.01). The expression of bd-2 mRNA was also decreased in SRRS group compared with the control (P<0.01).CONCLUSION: The inhibition of gastric cancer cell growth in vivo by Chinese Jianpi herbs and SRRS is related to induction of the cell apoptosis which may be involved in aberrant expression of p53 and bcl-2 | Ai-Guang Zhao Hai-Lei Zhao Jin-Kun Yang Lai-Di Tang Department of Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China Xiao-Jie Jin Renji Hospital,Shanghai Second Medical University,Shanghai 200032,China | 2002 | World Journal of Gastroenterology2002，8，5： | 43 |
| 2 | Cloning and characterization of squalene synthase and cycloartenol synthase from Siraitia grosvenorii显示文摘Mogrosides and steroid saponins are tetracyclic triterpenoids found in Siraitia grosvenorii.Squalene synthase(SQS) and cycloartenol synthase(CAS) are key enzymes in triterpenoid and steroid biosynthesis.In this study,full-length cDNAs of SgSQS and SgCAS were cloned by a rapid amplification of cDNA-ends with polymerase chain reaction(RACE-PCR) approach.The SgSQS cDNA has a 1254 bp open reading frame(ORF) encoding 417 amino acids,and the SgCAS cDNA contains a 2298 bp ORF encoding 765 amino acids.Bioinformatic analysis showed that the deduced SgSQS protein has two transmembrane regions in the C-terminal.Both SgSQS and SgCAS have significantly higher levels in fruits than in other tissues,suggesting that steroids and mogrosides are competitors for the same precursors in fruits.Combined in silico prediction and subcellular localization,experiments in tobacco indicated that SgSQS was probably in the cytoplasm or on the cytoskeleton,and SgCAS was likely located in the nucleus or cytosol.These results will provide a foundation for further study of SgSQS and SgCAS gene functions in S.grosvenorii,and may facilitate improvements in mogroside content in fruit by regulating gene expression. | Huan Zhao Qi Tang Changming Mo Longhua Bai Dongping Tu Xiaojun Ma | 2017 | Acta Pharmaceutica Sinica B2017，7，2： | 10 |
| 3 | Surface ozone observations during voyages to the Arctic and Antarctic regions显示文摘Surface ozone concentration and UV-B data between 75° N and 70° S were obtained aboard the Chinese polar scientific vessel 'Xue-long' (Snow-Dragon) during the first voyage to the Arctic and the 16th to the Antarctic in 1999-2000. Analysis of these data presents that variations of the surface ozone concentration have small amplitude during voyages except the mid-latitude in the Northern Hemisphere. As a whole, average surface ozone concentration in the Northern Hemisphere is higher than that in the Southern, and high value occurred when the ship sailed close to the continents. The average diurnal variations of the surface ozone in the Northern Hemisphere are also higher compared to the southern counterparts, and high diurnal variations were found at low latitudes, and relative low level in the polar region. | Longhua Lu Lingen Bian Yanjie Cheng Changgui Lu Jie Tang | 2001 | Chinese Science Bulletin2001，46，23： | 8 |
| 4 | Nitrogen-doped graphene nanosheets as high efficient catalysts for oxygen reduction reaction显示文摘It is of great significance in exploring alternative catalysts to platinum (Pt)-based materials for oxygen reduction reaction (ORR),because this reaction is invariably involved in various fuel cells and metal-air batteries.We herein reported the nitrogen doped graphene nanosheets (NGNSs) with pore volume of as high as 3.42 m 3 /g and investigated their potential application as ORR catalysts,it was demonstrated the NGNSs featured high activity,improved kinetics and excellent long-term stability for ORR.The NGNSs were successfully used as cathode catalysts of microbial fuel cells (MFCs) and performed even better than the commercial Pt/C (Pt 10%) catalysts at the maximum power output. | CI SuQin WU YongMin ZOU JianPing TANG LongHua LUO ShengLian LI JingHong WEN ZhenHai | 2012 | Chinese Science Bulletin2012，57，23： | 7 |
| 5 | Preparation and electrochemical performance for methanol oxidation of pt/graphene nanocomposites显示文摘 | LI Yueming TANG Longhua LI Jinghong | 2009 | Electrochemistry Communications2009，11，4： | 1 |
| 6 | Biosensor based on self-assembling glucose oxidase and dendrimer-encapsulated Pt nanopartieles on earbon nanotubes for glucose detection 显示文摘 | Xu Lihuan Zhu Yihua Tang Longhua | 2007 | Electroanalysis2007，19，： | 1 |
| 7 | Preparation and electrochemical performance for methanol oxidation of pt/graphene nanocomposites显示文摘 | Yueming Li Longhua Tang Jinghong Li | 2009 | Electrochemistry Communications2009，，4： | 1 |
| 8 | A glucose biosensor based on immobilization of glucose oxidase into 3D macroporous TiO2显示文摘 | Cao Huimin Zhu Yihua Tang Longhua Yang Xiaoling Li Chunzhong | 2008 | Electroanalysis2008，20，： | 1 |
| 9 | Preparation,structure and electrochemical properties of reduced graphene sheet films显示文摘 | TANG Longhua WANG Ying LI Yueming | | 0，，17： | 1 |
| 10 | Preparation and electro- chemical performance for methanol oxidation of pt/grapheme nano- composites 显示文摘 | Li Yueming Tang Longhua Li Jinghong | 2009 | Electrochemistry Communications2009，11，4： | 1 |
| 11 | Application of graphene-modified electrode for selective detection of dopamine显示文摘 | Ying Wang Yueming Li Longhua Tang Jin Lu Jinghong Li | 2009 | Electrochemistry Communications2009，，4： | 1 |
| 12 | Burning rate and flame tilt characteristics of radiation - controlled rectangular hydrocarbon pool fires with cross air flows in a reduced pressure 显示文摘 | Fei Tang Longhua Hu Xiaochun Zhang et 81 | 2015 | Fuel2015，139，： | 1 |
| 13 | Appli-cation of graphene-modified electrode for selective detec-tion of dopamine显示文摘 | WANG Ying LI Yueming TANG Longhua | 2009 | Electrochemistry Communications2009，11，4： | 1 |
| 14 | SelfGassembledgrapheneGenzymehierarchicalnanostrucGturesfor electrochemical biosensing 显示文摘 | Zeng Qiong ChengJinsheng Tang Longhua etal | 2010 | AdvancedFunctionalMaterials2010，20，19： | 1 |
| 15 | Prepara- tion, structure and electrochemical properties of graphene modified electrode 显示文摘 | Tang Longhua Wang Ying Li Yueming | 2009 | Adv Funct Mater2009，19，： | 1 |
| 16 | Preparation and electrochemical performance for methanol oxidation of Pt/ graphene nanocomposites 显示文摘 | LI Yueming TANG Longhua LI Jinghong | 2009 | Electrochem Commun2009，11，4： | 1 |
| 17 | Preparation and electrochemical performance for methanol oxidation of pt/graphene nanocomposites显示文摘 | Yueming Li Longhua Tang Jinghong Li | 2009 | Electrochemistry Communications2009，，4： | 1 |
| 18 | Treatment with Parenteral Nutrition Support and Chinese Herbs in One Case of Primary Small Intestinal Lymphangiectasia显示文摘INTESTINAL lymphangiectasia (IL) is a rare disease first reported by Waldmanin in 1961.1 Since then, no more than two hundred cases have been reported. | Yan-cheng Dai,Zhi-peng Tang,Gui-tong Ma,Ping Yin,Yu-ping Gong,Wen Liu,Song Wang,Ya-li Zhang,and Xin-ying He Department of Gastroenterology,Longhua Hospital Institute of Digestive Disease,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China | 2009 | Chinese Medical Sciences Journal2009，24，4： | 0 |
| 19 | Machine Learning-Aided Data Analysis in Single-Protein Conductance Measurement with Electron Tunneling Probes显示文摘The electrical tunneling sensors have excellent potential in the next generation of single-molecule measurement and sequencing technologies due to their high sensitivity and spatial resolution capabilities.Electrical tunneling signals that have been measured at a high sampling rate may provide detailed molecular information.Despite the extraordinarily large amount of data that has been gathered,it is still difficult to correlate signal transformations with molecular processes,which creates great obstacles for signal analysis.Machine learning is an effective tool for data analysis that is currently gaining more significance.It has demonstrated promising results when used to analyze data from single-molecule electrical measurements.In order to extract meaningful information from raw measurement data,we have combined intelligent machine learning with tunneling electrical signals.For the purpose of analyzing tunneling electrical signals,we investigated the clustering approach,which is a classic algorithm in machine learning.A clustering model was built that combines the advantages of hierarchical clustering and Gaussian mixture model clustering.Additionally,customized statistical algorithms were designed.It has been proven to efficiently gather molecular information and enhance the effectiveness of data analysis. | Yuxin Yang Tao Jiang Ye Tian Biaofeng Zeng Longhua Tang | 2024 | Chinese Journal of Chemistry2024，42，1： | 0 |
| 20 | Separation of nano-colloids in soils显示文摘 | Zhiyun TANG Longhua WU Yongming LUO | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 0 |
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