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| 1 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 2 | Validation of an algorithm combining haemoglobin A (1c) and fasting plasma glucose for diagnosis of diabetes mellitus in UK and Australian populations 显示文摘 | Manley SE Sikaris KA Lu ZX | 2009 | Diabet Med2009，26，2： | 1 |
| 3 | An automatic diagnosis method for the knee meniscus tears in MR images显示文摘 | K(o)se C Gen(c)alio(g)lu O (S)evik U | | 0，，02： | 1 |
| 4 | The effects of work- load on respiratory variables in simulated flight: a preliminary study显示文摘 | Karavidas MK Lehrer PM Lu SE | 2010 | Biological Psychology2010，84，1： | 1 |
| 5 | Glucocorticoids enhance cytotoxicity of cisplatin via suppression of NF-{kappa}B ac- tivation in the glucocorticoid receptor-rich human cervical carcinoma cell line SiHa显示文摘 | Lu YS Yeh PY Chuang SE eta/ | 2006 | J Endoerinol2006，188，2： | 1 |
| 6 | Glucocorticoids enhance cytotoxicity of cisplatin via suppression of NF-{kappa}B activation in the glucocorticoid receptor-rich human cervical carcinoma cell line SiHa显示文摘 | Lu YS Yeh PY Chuang SE | 2006 | J Endocrinol2006，188，2： | 1 |
| 7 | Constitutively active human Notchl binds to the transcription factor CBFI and stimulates transcription through a promoter containing a CBFl-responsive element 显示文摘 | Lu FM Lux SE | 1996 | Proc Natl Acad Sci USA1996，93，11： | 1 |
| 8 | Evidence that the gran- ulocyte colony-stimulating factor (G-CSF) receptor plays a role in the pharmacokinetics of G-CSF and PegG-CSF using a G- CSF-R KO model显示文摘 | Kotto-Komea AC Fox SE Lu W | 2004 | Pharmacol Res2004，50，1： | 1 |
| 9 | Simulation experiments on secondary thermal stress of biodegraded bituminous sandstones显示文摘Secondary variation of reservoir is a hot problem in petroleum geochemistry field. Several kinds of secondary variations have taken place after the formation of bituminous sandstones in the Tarim Basin including biodegra-dation, washing, dissipation and secondary thermal stress. Biodegraded bituminous sandstones were used in the experiment. Pyrolysis experiment has been performed in a closed system, simulating secondary thermal stress with continual burial of Silurian bituminous sandstones which may cause the changes in molecular compositions, carbon isotope and physical properties of bituminous sandstones. The results are as follows: (i) Gases are mainly product during the experiment and carbon isotope of gas is lighter. (ii) Yield of C6+ hydrocarbon is relative smaller, and yielded oil is mainly light oil. (iii) Chromatogram character of biodegraded oil-sand is similar to that of saturated hydrocarbon in extracts from present-day bituminous sandstones, (iv) Porosity of bituminous sandstones gradually | GONG Se PENG Ping’an LU Yuhong XIAO Zhongyao JIA Wanglu WANG Zhenqi YU Chiling LIU Dehan LU Jialan LIU Jinzhong | 2004 | Chinese Science Bulletin2004，49，S1： | 1 |
| 10 | Clinical manifestations, complications, and treatment of brucellosis: Evaluation of 72 cases显示文摘 | KSE S SENGER SS AKKOLU G | 2014 | Turkish J of Med Sci2014，44，： | 1 |
| 11 | Validation of an algorithm combining haemoglobin A(lc) and fasting plasma glucose for diagnosis of diabetes mellitus in UK and Australian populations显示文摘 | Manley SE Sikaris KA Lu ZX | 2009 | Diabetic Medicine2009，，2： | 1 |
| 12 | Follow-up of 89 asymptomatic patients with adrenoleukodystrophy treated with Lorenzo's oil显示文摘 | Moser HW Raymond GV Lu SE | 2005 | Arch Neurol2005，62，： | 1 |
| 13 | Heart rate variability biofeed- back effects of age on heart rate variability baroreflex gain and asthma显示文摘 | Lehrer P Vaschillo E Lu SE | 2006 | Chest2006，129，： | 1 |
| 14 | Sensitivity analysis of submerged arc welding process parameters显示文摘 | Serdar Karao?lu Abdullah Se?gin | 2007 | Journal of Materials Processing Tech2007，，1： | 1 |
| 15 | Heart rate variability biofeed back effects of age on heart rate variability, barore flex gain, and asthma显示文摘 | Lehrer P Vaschilb E Lu SE | 2006 | Chest2006，129，2： | 1 |
| 16 | Chronic exposure to secoisolariciresinol diglycoside alters lignan disposition in rats显示文摘 | Rickard SE Thompsofi LU | 1998 | J Nutr1998，128，3： | 1 |
| 17 | Follow-up of 89 asymptomatic patients with adrenoleukodystrophy treated with Lorenzo's oil 显示文摘 | Moser HW Raymond GV Lu SE | 2005 | Arch Neuro12005，62，7： | 1 |
| 18 | Microglial activation and recruitment, but not proliferation, suffice to mediate neurodegeneration 显示文摘 | Rogove AD Lu W Tsirka SE | 2002 | Cell Death Differ2002，9，8： | 1 |
| 19 | Glucocorticoids enhance cytotoxicity of cisplatin via suppression of NF-kappaB activation in the glucocorticoid receptor-rich human cervical carcinoma cell line SiHa显示文摘 | Lu YS Yeh PY Chuang SE | 2006 | J Endocrinol2006，188，2： | 1 |
| 20 | Microglial activation and recruitment,but not proliferation,suffice to mediate neurodegeneration显示文摘 | Lu W Tsirka SE | 2002 | Cell Death Differ2002，9，： | 1 |
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