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| 1 | Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer featured with high intra-tumoral heterogeneity and poor prognosis. To comprehensively delineate the PDAC intra-tumoral heterogeneity and the underlying mechanism for PDAC progression, we employed single-cell RNA-seq (scRNA-seq) to acquire the transcriptomic atlas of 57,530 individual pancreatic cells from primary PDAC tumors and control pancreases, and identified diverse malignant and stromal cell types, including two ductal subtypes with abnormal and malignant gene expression profiles respectively, in PDAC. We found that the heterogenous malignant subtype was composed of several subpopulations with differential proliferative and migratory potentials. Cell trajectory analysis revealed that components of multiple tumor-related pathways and transcription factors (TFs) were differentially expressed along PDAC progression. Furthermore, we found a subset of ductal cells with unique proliferative features were associated with an inactivation state in tumor-infiltrating T cells, providing novel markers for the prediction of antitumor immune response. Together, our findings provide a valuable resource for deciphering the intra-tumoral heterogeneity in PDAC and uncover a connection between tumor intrinsic transcriptional state and T cell activation, suggesting potential biomarkers for anticancer treatment such as targeted therapy and immunotherapy. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 24 |
| 2 | Observations of atmospheric pollutants at Lhasa during 2014-2015:Pollution status and the influence of meteorological factors显示文摘Atmospheric pollutants including SO_2, NO_2, CO, O_3 and inhalable particulate matter(PM2.5 and PM10) were monitored continuously from March 2014 to February 2015 to investigate characteristics of air pollution at Lhasa, Tibetan Plateau. Species exhibited similar seasonal variations except O_3, with the peaks in winter but low valleys in summer. The maximum O_3 concentration was observed in spring, followed by summer, autumn, and winter. The positive correlation between O_3 and PM10 in spring indicated similar sources of them, and was assumed to be turbulent transport. Temperature was the dominant meteorological factor for most species in spring. High temperature accelerates O_3 photochemistry, and favors air disturbance which is conductive to dust resuspension in spring. Relative humidity(RH) and atmospheric pressure were the main meteorological factors in summer. RH showed negative correlations with species, while atmospheric pressure posed opposite situation. Wind speed(WS) was the dominant meteorological factor in autumn, the negative correlations between WS and species indicated diffusion by wind. Most species showed non-significant correlations with meteorological factors in winter, indicating the dependence of pollution on source emission rather than restriction by meteorology. Pollution weather character indicated that emissions were from biomass burning and dust suspension, and meteorological factors also played an important role. Air stream injection from the stratosphere was observed during O_3 pollution period. Air parcels from Southwest Asia were observed during air pollution period in winter. An enhancement in air pollutants such as O_3 would be expected in the future, more attention should be given to countermeasures for prevention of air pollution in the future. | Bu Duo Lulu Cui Zhenzhen Wang Rui Li Liwu Zhang Hongbo Fu Jianmin Chen Huifang Zhang A.Qiong | 2018 | Journal of Environmental Sciences2018，30，1： | 9 |
| 3 | Characterization and acid-mobilization study for typical iron- bearing clay mineral显示文摘In this study, iron speciation in five standard clay samples was characterized. Iron mobilization from these clays was then measured in acidic media. For comparison, a commercially available Arizona test dust(ATD) was also observed. The results showed that the free-Fe contents of clays were commonly lower than that of dust aerosols. The components of clays were dominant by the structural Fe held in the aluminosilicate lattice.The iron solubility of the clays were in the order of KGa-2 > SWy-2 > CCa-2 > IMt-2 > NAu-2. Based upon the M?ssbauer spectrum and transmission electron microscopy(TEM)analysis, the Fe(II) fraction and the Fe/Si ratio of clay particles changed after dissolution,suggesting the total Fe solubility depended on the Fe atom states existing within the aluminosilicate lattice. The Fe in KGa-2 and SWy-2 was most likely substituted for alkaline elements as the interlayer ions held by ionic bonds in the aluminosilicate, which are more liable to dissolution. However, the Fe in NAu-2 was more likely to be bound by strong covalent bonds in aluminosilicate mineral, which is less soluble. The much highly soluble Fe in ATD was not only linked to the dissolution of an appreciable fraction of Fe(II), but also could be attributed to the fact that the Fe bonds in the clay fraction of ATD were mainly present as ionic bonds. The TEM images showed that reacted clay particles displayed less aggregate particles, with nanoparticle aggregates and the Fe/S-rich tiny particles attached to the remains. | Zhenzhen Wang Rui Li Lulu Cui Hongbo Fu Jun Lin Jianmin Chen | 2018 | Journal of Environmental Sciences2018，30，9： | 4 |
| 4 | Pressure Fluctuation Generated by the Interaction of Blade and Tongue显示文摘Pressure fluctuation around the tongue has large effect on the stable operation of a centrifugal pump. In this paper, the Reynolds averaged Navier-Stokes equations(RANS) and the RNG k-epsilon turbulence model is employed to simulate the flow in a pump. The flow field in the centrifugal pump is computed for a range of flow rate. The simulation results have been compared with the experimental data and good agreement has been achieved. In order to study the interaction of the tongue with the impeller, fifteen monitor probes are evenly distributed circumferentially at three radii around the tongue. Pressure distribution is investigated at various blade positions while the blade approaches to and leaves the tongue region. Results show that pressure signal fluctuates largely around the tongue, and it is more intense near the tongue surface. At design condition, standard deviation of pressure fluctuation is the minimum. At large flow rate, the increased low pressure region at the blade trailing edge results in the increases of pressure fluctuation amplitude and pressure spectra at the monitor probes. Minimum pressure is obtained when the blade is facing to the tongue. It is found that the amplitude of pressure fluctuation strongly depends on the blade positions at large flow rate, and pressure fluctuation is caused by the relative movement between blades and tongue. At small flow rate, the rule of pressure fluctuation is mainly depending on the structure of vortex flow at blade passage exit besides the influence from the relative position between the blade and the tongue. | ZHENG Lulu DOU Hua-Shu CHEN Xiaoping ZHU Zuchao CUI Baoling | 2018 | Journal of Thermal Science2018，27，1： | 4 |
| 5 | Effects of Different Dietary Energy and Protein Levels on Meat Performance and Meat Quality of Jinghai Yellow Chickens显示文摘To investigate the effect of different dietary energy and protein levels on meat performance and meat quality of Jinghai yellow chickens,480 43-day old Jinghai yellow chickens with similar weight were randomly divided into four experimental groups: experimental group 1( protein 15%,metabolic energy 9. 95 MJ /kg),experimental group 2( protein 16%,metabolic energy 10. 95 MJ /kg),experimental group 3( protein 17%,metabolic energy 12. 65 MJ /kg) and experimental group 4( protein 18%,metabolic energy 13. 95 MJ /kg),respectively. All chickens were slaughtered at 112-day old. The breast and leg muscles of Jinghai yellow chickens were collected,to determine the slaughter performance,conventional meat quality and muscle chemical indicators. The results indicated that dressing-out percentage and eviscerated yield percentage in four experimental groups were above 87. 27% and 67. 00%,respectively; other slaughter performance indicators exhibited no significant differences among various groups( P > 0. 05); breast muscle color of hens in experimental group 4 varied significantly from that in other three groups( P < 0. 05); leg muscle color of hens in experimental group 2 varied extremely significantly from that in other three groups( P < 0. 01); water-holding capacity of breast muscles of hens in experimental group 3 was significantly higher than that in experimental group 4( P < 0. 05); thiamine content of breast muscles of cocks in experimental group 3 was significantly higher than that in experimental group 2( P < 0. 05); however,other properties exhibited no significant differences among various groups( P > 0. 05). | Jianfeng WANG Lulu CUI Kaizhou XIE Yingying SUN Lirui SUN Jianyu LIU Genxi ZHANG Guojun DAI Jinyu WANG | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
| 6 | Research on Copyright Protection Method of Material Genome Engineering Data Based on Zero-Watermarking显示文摘In order to effectively solve the problem of copyright protection of materials genome engineering data,this paper proposes a method for copyright protection of materials genome engineering data based on zero-watermarking technology.First,the important attribute values are selected from the materials genome engineering database;then,use the method of remainder to group the selected attribute values and extract eigenvalues;then,the eigenvalues sequence is obtained by the majority election method;finally,XOR the sequence with the actual copyright information to obtain the watermarking information and store it in the third-party authentication center.When a copyright dispute requires copyright authentication for the database to be detected.First,the zero-watermarking construction algorithm is used to obtain an eigenvalues sequence;then,this sequence is XORed with the watermarking information stored in the third-party authentication center to obtain copyright information to-be-detected.Finally,the ownership is determined by calculating the similarity between copyright information to-be-detected and copyright information that has practical significance.The experimental result shows that the zero-watermarking method proposed in this paper can effectively resist various common attacks,and can well achieve the copyright protection of material genome engineering database. | Lulu Cui Yabin Xu | 2020 | Journal on Big Data2020，2，2： | 1 |
| 7 | Fabrication and Charac- terization of A Double-Heater Based MEMS Thermal Flow Sensor 显示文摘 | Sun Jianhai Cui Dafu Zhang Lulu | 2013 | Sensors and Actuators A2013，193，： | 1 |
| 8 | Folate-conjugated pH-responsive nanocarrier designed for active tumor targeting and controlled release of doxorubicin显示文摘 | Lulu WEI Beibei LU Lin CUI Xueying PENG Jianning wu Deqiang LI Zhiyong LIU Xuhong GUO | 2017 | Frontiers of Materials Science2017，11，4： | 1 |
| 9 | Author Correction:Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma显示文摘In the initial published version of this Article,there was an error in author information. Da-Li Han (handl@big.ac.cn) is a co-first author and senior author. This correction does not affect the description of the results or the conclusions of this work. | Junya Peng Bao-Fa Sun Chuan-Yuan Chen Jia-Yi Zhou Yu-Sheng Chen Hao Chen Lulu Liu Dan Huang Jialin Jiang Guan-Shen Cui Ying Yang Wenze Wang Dan Guo Menghua Dai Junchao Guo Taiping Zhang Quan Liao Yi Liu Yong-Liang Zhao Da-Li Han Yupei Zhao Yun-Gui Yang Wenming Wu | 2019 | Cell Research2019，29，9： | 0 |
| 10 | Ultrathin origami accordion-like structure of vacancy-rich graphitized carbon nitride for enhancing CO_(2) photoreduction显示文摘Retaining the ultrathin structure of two-dimensional materials is very important for stabilizing their catalytic performances.However,aggregation and restacking are unavoidable,to some extent,due to the van der Waals interlayer interaction of two-dimensional materials.Here,we address this challenge by preparing an origami accordion structure of ultrathin twodimensional graphitized carbon nitride(oa-C_(3)N_(4))with rich vacancies.This novel structured oa-C_(3)N_(4) shows exceptional photocatalytic activity for the CO_(2) reduction reaction,which is 8.1 times that of the pristine C_(3)N_(4).The unique structure not only prevents restacking but also increases light harvesting and the density of vacancy defects,which leads to modification of the electronic structure,regulation of the CO_(2) adsorption energy,and a decrease in the energy barrier of the carbon dioxide to carboxylic acid intermediate reaction.This study provides a new avenue for the development of stable highperformance two-dimensional catalytic materials. | Guangri Jia Zhongxu Wang Ming Gong Ying Wang Lu Hua Li Yilong Dong Lulu Liu Lei Zhang Jingxiang Zhao Weitao Zheng Xiaoqiang Cui | 2023 | Carbon Energy2023，5，4： | 0 |
| 11 | LPS-induced mitochondrial DNA synthesis and release facilitate RAD50-dependent acute lung injury显示文摘Dear Editor,ATP-binding cassette(ABC)-ATPase(RAD50),together with meiotic recombination 11 homolog 1(MRE11)subunits,to form MRE11-RAD50 complex,plays important roles in recognition of double-stranded DNA(dsDNA)and initiation of consequent inflammatory cascade1.Acute lung injury(ALI)and acute respiratory destress syndrome(ARDS)are systemic uncontrolled inflammation and life-threatening.However,the function of the DNA sensor in ALI/ARDS remains poorly defined.Here we investigated functions of RAD50 using mouse primary macrophages and conditionally RAD50 knockout mice in vitro and in a lipopolysaccharide(LPS)-induced lung injury model. | Xueqin Zhan Rui Cui Xinwei Geng Jiaqian Li Yunlian Zhou Lulu He Chao Cao Chao Zhang Zhimin Chen Songmin Ying | 2021 | Signal Transduction and Targeted Therapy2021，6，4： | 0 |
| 12 | Analysis on Irrational Decision Making of Tobacco Family Farmers from the Perspective of Optimal Scale of Efficiency显示文摘The larger the difference between the willingness scale of tobacco family farmers and the optimal scale of efficiency,the greater the degree of irrationality,and the higher the decision making risk.With the aid of DEA model,this study calculated the optimal scale of efficiency of Guiyang tobacco family farms.Using the ratio of willingness scale and efficiency optimal scale,it measured the degree of irrationality of family farmers.In addition,with the help of multiple linear regression model,it explained the irrational decision making mechanism of family farmers.Finally,it made a portrait of farmers who tend to make irrational decisions,to find specific farmers and guide them in their production and operation,reduce the risk of planting scale decision making and stabilize the sustainable development of the tobacco industry. | Ya nan SUN Lulu CUI Baohua YU | 2022 | Asian Agricultural Research2022，14，9： | 0 |
| 13 | Targeted drug delivery strategies for the treatment of rheumatoid arthritis显示文摘A chronic autoimmune disease named rheumatoid arthritis(RA)is characterized mainly by synovitis,which has high morbidity,disability and mortality rates.In addition,the aetiology and pathogenesis of RA have not been fully determined.RA is usually accompanied by persistent arthritic pain,swelling,stiffness,and functional disorders;in severe cases,it may result in cardiovascular,pulmonary,psychological and skeletal diseases(Scott et al.,2010). | Jinxia Zhang Ling Jiang Lihong Sun Ping Wang Suhui Sun Menghong Xu Lulu zhang Shumin Wang Xiaolong Liang Ligang Cui | 2021 | Science China(Life Sciences)2021，64，7： | 0 |
| 14 | Isolation of plasma from whole blood using a microfludic chip in a continuous cross-flow显示文摘A novel microfluidic chip is developed for crossflow filtration plasma from the whole blood which is carried out in a continuous manner. This microfluidic chip was made of a silicon substrate sealed with a compound cover. The silicon substrate fabricated by micro-electro-mechanical system (MEMS) technology consisted of microposts array, microchannels and reservoirs. Then the silicon substrate was characterized by Scaning Electron Microscopy (SEM). The performance of the microfluidic chip was valued by the experiments of plasma isolation. During more than one hour of continuous blood infusion through the chip, there were no problems of jamming or clogging, and the plasma selectivity of 97.78% was achieved. Due to the chip’s simple structure and control mechanism with a continuous, real time operating manner, this microfluidic chip is easily expected to be integrated into micro total analytical system (μTAS) which will create a microanalysis system for point-of-care diagnostics. | CHEN Xing CUI DaFu ZHANG LuLu | 2009 | Chinese Science Bulletin2009，54，2： | 0 |
| 15 | Paternally imprinted LATE-FLOWERING2 transcription factor contributes to paternal-excess interploidy hybridization barriers in wheat∞显示文摘Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution,and is commonly employed in wheat breeding programs.Hexaploid wheat usually serves as maternal parent because the reciprocal cross generates progeny with severe defects and poor seed germination,but the underlying mechanism is poorly understood.Here,we performed detailed analysis of phenotypic variation in endosperm between two interploidy reciprocal crosses arising from tetraploid(Triticum durum,AABB)and hexaploid wheat(Triticum aestivum,AABBDD).In the paternal‐versus the maternal‐excess cross,the timing of endosperm cellularization was delayed and starch granule accumulation in the endosperm was repressed,causing reduced germination percentage.The expression profiles of genes involved in nutrient metabolism differed strongly between these endosperm types.Furthermore,expression patterns of parental alleles were dramatically disturbed in interploidy versus intraploidy crosses,leading to increased number of imprinted genes.The endosperm‐specific TaLFL2 showed a paternally imprinted expression pattern in interploidy crosses partially due to allele‐specific DNA methylation.Paternal TaLFL2 binds to and represses a nutrient accumulation regulator TaNAC019,leading to reduced storage protein and starch accumulation during endosperm development in paternal‐excess cross,as confirmed by interploidy crosses between tetraploid wild‐type and clustered regularly interspaced palindromic repeats(CRISPR)–CRISPR‐associated protein 9 generated hexaploid mutants.These findings reveal a contribution of genomic imprinting to paternal‐excess interploidy hybridization barriers during wheat evolution history and explains why experienced breeders preferentially exploit maternal‐excess interploidy crosses in wheat breeding programs. | Guanghui Yang Man Feng Kuohai Yu Guangxian Cui Yan Zhou Lv Sun Lulu Gao Yumei Zhang Huiru Peng Yingyin Yao Zhaorong Hu Vincenzo Rossi Ive De Smet Zhongfu Ni Qixin Sun Mingming Xin | 2023 | Journal of Integrative Plant Biology2023，65，12： | 0 |
| 16 | Effect of Mitochondrial Function of Ovarian Granulosa Cells on In Vitro Fertilization and Embryo Transfer Outcomes in Obese Polycystic Ovary Syndrome Patients显示文摘Objective:To investigate the effect of abnormal ovarian granulosa cell metabolism on in vitro fertilization and embryo transfer(IVF-ET)outcomes in obese polycystic ovary syndrome(PCOS)patients.Methods:Patients with PCOS who met the study criteria were screened according to the inclusion criteria.A total of 32 patients with obese PCOS were recruited into the study group,and 39 patients with non-obese PCOS were recruited into the control group.The general data(age,body mass index,and years of infertility),insulin resistance index(HOMA-IR),follicle-stimulating hormone(FSH),luteinizing hormone(LH),granulosa cell mitochondrial function,and IVF-ET outcome of patients in the study group and control group were retrospectively analyzed.Results:The differences in age and years of infertility between the study group and the control group were insignificant(P>0.05),and the body mass index(BMI)of the study group and control group was 30.5±1.24 kg/m2 and 22.3±1.12 kg/m2,respectively,in which the difference was statistically significant(P<0.05);the HOMA-IR of the study group was significantly higher than that of the control group(P<0.05);the reactive oxygen species(ROS)in the study group was significantly higher than that in the control group(P<0.05),and the ATP content in the study group was significantly lower than that in the control group(P<0.05);comparing the FSH and LH levels between the two groups,the difference was not statistically significant(P>0.05);the rate of IVF-ET failure was significantly higher in the study group than in the control group.Conclusion:PCOS is a complex endocrine disorder,and obesity is one of the independent risk factors for the development of PCOS. | Jing Wang Yana Gao Hongli Wu Gaijing Wang Jie Cui Jinjin Qin Lulu Wang Yakun Zhao Rui Li | 2023 | Proceedings of Anticancer Research2023，7，3： | 0 |
| 17 | Blood compatible heteratom-doped carbon dots for bio-imaging of human umbilical vein endothelial cells显示文摘Carbon dots have unique advantages in biological applications owing to their excellent optical prope rties.However,the biosafety evaluation of carbon dots has limitations owing to cytotoxicity in vitro,and the re is little pre-safety evaluation before in vivo and clinical applications.Whether the carbon dots are or not suitable for applications in vivo,evaluation analysis can be made based on hemolysis and changes in erythrocyte morphology.In this work,a green fluorescent N,S-doped carbon dots(N,S-CDs)were obtained by hydrothermal method,tobias acid,and m-phenylenediamine as precursors.N,S-CDs not only possessed excellent dispersibility,uniform particle size,high quantum yield(37.2%)and stable photoluminescence property but also retain their photostability and stro ng fluorescence intensity in the acid/alkaline solutions,different ionic strengths(NaCl)and under 365 nm UV illumination.Moreove r,the N,S-CDs displayed low cytotoxicity and high cellular uptake efficiency in human umbilical vein endothelial cells(HUVEC)and excellent blood compatibility to the erythrocyte.It is foreseeable that N,S-CDs could be further studied as a promising biological imaging agent in vivo. | Jian Zhong Xinmian Chen Miaoran Zhang Chaoxin Xiao Lulu Cai Waleed Ali Khan Kaixuan Yu Jiayi Cui Lin He | 2020 | Chinese Chemical Letters2020，31，3： | 0 |
| 18 | High permittivity of Ba(Ti_(1-x)Zr_(x))O_(3)-based Y5V-type nanopowders and ceramics synthesized using a one-step sol-gel method显示文摘We synthesized Ba(Ti_(1-x)Zr_(x))O_(3)-based(BTZ)nanopowders modified with Cu and Nb,and sintered ceramics using a one-step sol-gel process.Characterization using TG-DTG,XRD,SEM,and TEM analysis methods revealed that the powders formed at a nanometer scale.The process was controlled by the composition and calcining temperature.BaZrO3 formed earlier than BaTiO3 during the calcining,then a Ba(Ti_(1-x)Zr_(x))O_(3) solid solution formed incompletely,resulting in ceramics with huge permittivity and good temperature stability.Moreover,the maximum dielectric constant(εmax)first increased and then decreased as the Curie temperature(Tc)shifted to a lower temperature.When the Zr concentration was 5.4 mol%,εmax was greater than 24,000 and the dielectric loss(25℃)was only 0.005;this meets the Electronic Industries Alliance Y5V specifications.These nanopowders would permit a significant reduction in the thickness of multilayer ceramic capacitors and the huge pemittivity of the ceramics will help meet the current demand for device miniaturization in the electronics industry. | Lulu Zhang Bin Cui Rong Ma Leilei Li Yan Wang Wenqing Yan Zhuguo Chang | 2013 | Journal of Advanced Dielectrics2013，3，4： | 0 |
| 19 | Graphene quantum dots doped poly(vinyl alcohol)hybrid membranes for desalination via pervaporation显示文摘Pervaporation desalination by highly hydrophilic materials such as poly(vinyl alcohol)(PVA)based separation membrane is a burgeoning technology of late years.However,the improvement of membrane flux in pervaporation desalination has been a difficult task.Here,a novel hybrid membrane with doped graphene oxide quantum dots(GOQDs)which is rich in hydrophilic groups and small size into the matrix of PVA was prepared to improve the membrane flux.The membranes structures were described by field emission scanning electron microscopy(FESEM),atomic force microscopy(AFM),Fourier transform infrared(FT-IR),differential scanning calorimetry(DSC),thermogravimetric analysis(TGA)and X-ray diffraction(XRD).And more,Water contact angle,swelling degree,and pervaporation properties were carried out to explore the effect of GOQDs in PVA matrix.In addition,GOQDs content in the hybrid membrane,NaCl concentration,and feed temperature were investigated accordingly.Moreover,the hydrogen bonds between PVA chains were weakened by the interaction between GOQDs and PVA chains.Significantly,the hybrid membrane with optimized doped GOQDs content,200 mg·L^(-1),displays a high membrane flux of 17.09 kg·m^(-2)·h^(-1)and the salt rejection is consistently greater than 99.6%. | Yunyun Wan Lulu Yao Peng Cui | 2023 | Chinese Journal of Chemical Engineering2023，63，11： | 0 |
| 20 | Comparative transcriptional analysis and identification of hub genes associated with wing differentiation of male in Aphis gossypii显示文摘Background:Aphis gossypii Glover(Hemiptera:Aphididae),a worldwide polyphagous phloem-feeding agricultural pest,has three wing morphs(winged parthenogenetic female,gynopara,and male)in the life cycle.The exclusive males could fly from summer hosts to winter hosts,which are essential for gene exchanges of cotton aphid populations from different hosts or regions.However,the molecular mechanism of wing differentiation of male in A.gossypii remains unclear.Results:Morphological observation of male A.gossypii showed that there is no distinct difference in the external morphologies of the 1st and 2nd instar nymphs.The obvious differentiation of wing buds started in the 3rd instar nymph and was visible via naked eyes in the 4th instar nymphal stage,then adult male emerged with full wings.According to morphological dynamic changes,the development of wings in males were divided into four stages:preliminary stage(the 1st instar to 2nd instar),prophase(the 3rd instar),metaphase(the 4th instar),anaphase(the 5th instar).Results of feeding behavior monitoring via EPG(electrical penetration graph)technology indicated that although the male cotton aphids had strong desire to feed(longer duration of C 55.24%,F 5.05%and Pd waves 2.56%),its feeding efficiency to summer host cotton was low(shorter E13.56%and E2 waves 2.63%).Dynamic transcriptome analysis of male aphid at 5 different developmental periods showed that in the 3rd instar nymph,the number of up-regulated DEGs was significant increased,and time-course gene transcriptional pattern analyses results also showed that numerous genes categorized in clusters 3,5,and 8 had the highest expressed levels,which were consistent with morphological changes of wing buds.These results indicate that the 3rd instar nymph is the critical stage of wing bud differentiation in males.Furthermore,through pathway enrichment analysis of DEGs and WGCNA,it revealed that the neuroactive ligand-receptor interaction,Ras signaling pathway,dopaminergic synapse,circadian entrainment and the corresponding hub genes of PLK1,BUB1,SMC2,TUBG,ASPM,the kinesin family members(KIF23,KIF20,KIF18-19)and the novel subfamily of serine/threonine(Aurora kinase A and Aurora kinase B)probably played an important role in the critical stage of wing bud differentiation.Conclusion:This study explored morphological changes and genes transcriptional dynamics males in cotton aphid,revealed the phenomenon of low feeding efficiency of winged males on summer host cotton,and identified key signaling pathways and potential hub genes potentially involved in wing bud differentiation of male in A.gossypii. | HUANGFU Ningbo SHI Qingyu CHEN Lulu MA Xiaoyan ZHANG Kaixin LI Dongyang WANG Li ZHU Xiangzhen JI Jichao LUO Junyu CUI Jinjie | 2022 | Journal of Cotton Research2022，5，3： | 0 |
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